Соціальний туризм в Україні: проблеми та перспективи розвитку

Метою роботи є дослідження сегментації соціального туризму, розгляд існуючих сегментів соціального туризму в Україні, визначення проблем та перспектив розвитку

Роль сечової кислоти в механізмах кардіоінотропних ефектів бальнеотерапевтичного комплексу курорту Трускавець

В русле концепции оо эндогенной мочевой кислоте как одном из звеньев механизма действия биоактивной воды Нафтуся на функциональные системы организма проанализированы совместные изменения под влиянием бальнеотерапии на курорте Трускавець уровня урикемии и сократительной активности миокарда. Выявлены три типа детерминации урикемией инотропии: ураторто-, уратинверс- и уратнезависимый типы, реализация которых обуславливается констелляцией 22 исходных параметров вегетативной регуляции, велоэргометрии, электролитного и липидного обменов, гемодинамики, а также антропометрии.It is detected three types determination of inotropy by plasma level of uric acid: uratorthodependent, uratinversdependent and uratindependent types. It is selected constellation of initial parameters of vegetative regulation, veloergometry, haemodynamic, antropometry, electrolythic and lipid exchange conditionizes these types

Крымский юридический - от командных курсов РКМ к институту МВД: этапы становления и развития (1921-2006 гг.)

Новизна данной статьи состоит в первую очередь, в постановке самой проблемы и решении ряда конкретных исследовательских задач, касающихся организационного построения и деятельности учебного заведения системы Министерства внутренних дел (МВД) Украины в Крыму. В представленной работе, на основе архивных документов и материалов, значительная часть которых вводится в научный оборот впервые, автором исследуются различные стороны исторического развития Крымского юридического института Харьковского национального университета внутренних дел.Новизна даної статті полягає в першу чергу, в постановці самої проблеми і рішенні ряду конкретних дослідницьких задач, що стосуються організаційної побудови і діяльності учбового закладу системи Міністерства внутрішніх справ (МВС) України в Криму. У представленій роботі, на основі архівних документів і матеріалів, значна частина яких вводиться в науковий оборот вперше, автором досліджуються різні сторони історичного розвитку Кримського юридичного інституту Харківського національного університету внутрішніх справ

Effect of ochratoxin A on the intestinal mucosa and mucosa-associated lymphoid tissues in broiler chickens

The immunotoxic effect of ochratoxin A (OTA) on the intestinal mucosa-associated lymphoid tissue and its cytotoxic action on the intestinal epithelium were studied in broiler chickens experimentally treated with the toxin. From the 7th day of life, 80 male broiler chickens (Ross 308) were randomly divided into four groups of 20 birds each. The three experimental groups (E1-3) were treated with OTA for 28 days (E1: 50 μg/kg body weight [bw]/day; E2: 20 μg/kg bw/day; E3: 1 μg/kg bw/day) and the fourth group served as control. Histological examination of the intestinal mucosa and immunohistochemical staining for identification of CD4+, CD8+, TCR1 and TCR2 lymphocytes in the duodenum, jejunum and ileocaecal junction were performed, and CD4+/CD8+ and TCR1/TCR2 ratios were calculated. OTA toxicity resulted in decreased body weight gain, poorer feed conversion ratio, lower leukocyte and lymphocyte count, and altered intestinal mucosa architecture. After 14 days of exposure to OTA, immunohistochemistry showed a significant reduction of the lymphocyte population in the intestinal epithelium and the lamina propria. After 28 days of exposure, an increase in the CD4+ and CD8+ values in both the duodenum and jejunum of chickens in Groups E1 and E2 was observed, but the TCR1 and TCR2 lymphocyte counts showed a significant reduction. No significant changes were observed in Group E3. The results indicate that OTA induced a decrease in leukocyte and lymphocyte counts and was cytotoxic to the intestinal epithelium and the mucosa-associated lymphoid tissue, altering the intestinal barrier and increasing susceptibility to various associated diseases

A broader role for AmyR in Aspergillus niger: regulation of the utilisation of d-glucose or d-galactose containing oligo- and polysaccharides

AmyR is commonly considered a regulator of starch degradation whose activity is induced by the presence of maltose, the disaccharide building block of starch. In this study, we demonstrate that the role of AmyR extends beyond starch degradation. Enzyme activity assays, genes expression analysis and growth profiling on d-glucose- and d-galactose-containing oligo- and polysaccharides showed that AmyR regulates the expression of some of the Aspergillus niger genes encoding α- and β-glucosidases, α- and β- galactosidases, as well as genes encoding α-amlyases and glucoamylases. In addition, we provide evidence that d-glucose or a metabolic product thereof may be the inducer of the AmyR system in A. niger and not maltose, as is commonly assumed

Expression of drug efflux transporters in poultry tissues.

Multidrug resistance 1 (MDR1) and multidrug resistance-associated protein 2 (MRP2) are two prominent members of the super-family of ATP-binding cassette (ABC) transporters that carry a wide range of substrates across biological membranes, using ATP as energy source. The level of expression of these efflux transporters in different tissues has hitherto been studied mainly in mammals, and only P-glycoprotein (P-gp), the product of the MDR1 gene, has been described in chickens as of yet. The aim of this study was to describe the levels of expression of MDR1 and MRP2 mRNAs in different tissues of chickens, as these transporters play an important role in the absorption, distribution and excretion of drugs and toxins. In the gastro-intestinal tract, the highest levels of MDR1 mRNA expression were found in the small intestines, followed by the colon, whereas lower levels were found in the crop, proventriculus and the caeca. High MDR1 expression was also measured in the excretory organs such as liver, kidney and lungs. In contrast to rodents and humans, relatively low levels were found in the adrenals and in the immature sex organs such as testicles and ovaries. MRP2 mRNA expression was high in the liver, kidneys, duodenum and the jejunum, but expression was low in the ileum as well as in the lungs. No MRP2 expression could be detected in the other organs tested. Comparing the findings in chickens with previously published data, in particular those from humans and rodents, an unexpected high degree of similarity in the expression pattern of MDR1 and MRP2 mRNAs was apparent