Pontos de vista sobre percepção e ação no treinamento do ator: Viewpoints em questão

Abordagem do treinamento Viewpoints (Pontos de Vista), organizado pela diretora americana Anne Bogart, enquanto alternativa aos modelos tradicionais de atuação e direção teatral. Estudo sobre percepção e relação corpo-ambiente no treinamento do ator e bailarino a partir de ações físicas e vocais. Análise de experiências realizadas no grupo de pesquisa O corpomente em cena: as ações físicas do ator/bailarino baseadas na percepção e consciência do estado do corpo no espaço e tempo propostos pelo Viewpoints

Vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration

The contribution of decameric vanadate species to vanadate toxic effects in cardiac muscle was studied following an intravenous administration of a decavanadate solution (1 mM total vanadium) in Sparus aurata. Although decameric vanadate is unstable in the assay medium, it decomposes with a half-life time of 16 allowing studying its effects not only in vitro but also in vivo. After 1, 6 and 12 h upon decavanadate administration the increase of vanadium in blood plasma, red blood cells and in cardiac mitochondria and cytosol is not affected in comparison to the administration of a metavanadate solution containing labile oxovanadates. Cardiac tissue lipid peroxidation increases up to 20%, 1, 6 and 12 h after metavanadate administration, whilst for decavanadate no effects were observed except 1 h after treatment (+20%). Metavanadate administration clearly differs from decavanadate by enhancing, 12 h after exposure, mitochondrial superoxide dismutase (SOD) activity (+115%) and not affecting catalase (CAT) activity whereas decavanadate increases SOD activity by 20% and decreases ( 55%) mitochondrial CAT activity. At early times of exposure, 1 and 6 h, the only effect observed upon decavanadate administration was the increase by 20% of SOD activity. In conclusion, decavanadate has a different response pattern of lipid peroxidation and oxidative stress markers, in spite of the same vanadium distribution in cardiac cells observed after decavanadate and metavanadate administration. It is suggested that once formed decameric vanadate species has a different reactivity than vanadate, thus, pointing out that the differential contribution of vanadium oligomers should be taken into account to rationalize in vivo vanadate toxicity

Cambios en la composición de la captura y de la captura accesoria y en diversidad específica en una pesquería con nasas camaroneras semi-flotantes en tres ecosistemas insulares del Atlántico oriental sujetos a distinta presión antrópica

Composition and bycatch of semi-floating shrimp-trap fisheries (SSTF) were compared among areas with different levels of anthropogenic alteration of marine ecosystems. The three areas selected were Madeira, the Canary Islands and Cape Verde. Mean species richness and diversity of the SSTF did not show significant differences among areas. The dominant species in catches of the SSTF for all regions studied was the main target species, Plesionika edwardsii, which accounted for 96.0% of the catch in Cape Verde, 75.8% in Madeira and 59.1% in the Canary Islands. Targeted pandalid shrimps accounted for more than 96.8% of total catches for all areas combined. Numbers of non-target species caught were 18 (Madeira), 14 (Canary Islands) and 16 (Cape Verde), of which 13 (Madeira), 8 (Canary Islands) and 11 (Cape Verde) were always discarded. Bycatch accounted for 0.5% (Madeira), 0.7% (Canary Islands) and 3.1% (Cape Verde) in numbers. Shark species accounted for 0.11% of all individuals caught. A total of 5 species in Madeira, 6 in the Canary Islands and 4 in Cape Verde, accounting for 0.2% to 0.8% of total catches, were not landed due to the small size of individuals or low numbers of individuals caught (self-consumption). The present results suggest that the selectivity of traps for the main target species, P. edwardsii, in SSTF changes due to changes in species dominance, which are probably linked to the degree of human fishing exploitation of the marine ecosystems in each area.Se comparó la composición de las capturas y la captura accesoria de la pesquería de camarones con nasas semi-flotantes (SSTF) entre áreas con diferentes niveles de alteración de sus ecosistemas marinos por la acción humana. Se seleccionaron tres áreas: Madeira, Canarias y Cabo Verde. La riqueza de especies y la diversidad de la pesquería no mostraron diferencias significativas entre áreas. La especie dominante en las capturas de la SSTF fue la principal especie objetivo, Plesionika edwardsii, en las tres regiones estudiadas, variando desde 96.0% (Cabo Verde) hasta 59.1% (Canarias), con 75.8% en Madeira. Los camarones pandálidos objetivo de la pesca representaron más del 96.8% de la captura total en el conjunto de las tres áreas. El número de especies no-objetivo capturadas fue 18 (Madeira), 14 (Canarias) y 16 (Cabo Verde), de las cuales siempre fueron descartadas 13 especies en Madeira, 8 en Canarias y 11 en Cabo Verde. El porcentaje de individuos (en número) de las capturas accesorias en relación con las capturas totales representó el 0.5% en Madeira, 0.7% en Canarias y 3.1% en Cabo Verde. Las especies de tiburones representaron solo el 0.11% de los individuos capturados. Cinco especies en Madeira, seis en Canarias y cuatro en Cabo Verde representaron entre 0.2 y 0.8% del total de las capturas no desembarcadas debido a su pequeño tamaño o al bajo número de individuos capturados (auto consumo). Los resultados obtenidos sugieren que las nasas de la SSTF cambian sus características de selectividad con respecto a la especie objetivo principal, P. edwardsii, debido a cambios relativos entre las especies dominantes, lo que probablemente está relacionado con la explotación pesquera de los ecosistemas marinos en cada área

Increased CD3+, CD8+, or FoxP3+ T lymphocyte infiltrations are associated with the pathogenesis of colorectal cancer but not with the overall survival of patients

Tumor-infiltrating lymphocytes include heterogeneous populations of T lymphocytes that play crucial roles in the tumor immune response; importantly, their presence in the tumor tissue may predict clinical outcomes. Therefore, we herein studied the prognostic significance of the presence and location of CD3+, CD8+, and FoxP3+ T lymphocytes in colorectal cancer samples. In the intratumor analysis, our data did not reveal any association between lymphocyte infiltrations with clinical or pathological data. However, in the tumor margins, we found that the presence of high infiltrations of CD3+, CD8+, or FoxP3+ T lymphocytes were associated with TNM stages I-II (p = 0.021, p = 0.022, and p = 0.012, respectively) and absence of lymph node metastases (p = 0.010, p = 0.003, and p = 0.004, respectively). Despite these associations with good prognostic indicators, we were not able to find any statistically significant alterations in the overall survival of the patients, even though high infiltrations of FoxP3+ T lymphocytes in the tumor margins resulted in an increased overall survival of 14 months. Taken together, these data show that the presence of CD3+, CD8+, or FoxP3+T lymphocyte infiltrates in the tumor margins are associated with the pathogenesis of CRC, but only high Foxp3+ T lymphocyte infiltrations in the tumor invasive margins are inclined to indicate favorable prognosis.This work was supported by National funds through the Foundation for Science and Technology (FCT)—projects PTDC/MED-ONC/28658/2017, UIDB/50026/2020, and UIDP/50026/2020; and by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) projects NORTE-01-0145-FEDER-000013 and NORTE-01-0145-FEDER-000023. AMB was supported by the FCT fellowship SFRH/BD/120371/2016 and ET by the FCT investigator grant IF/01390/2014 and Estímulo Individual ao Emprego Científico CEECIND/03070/2020

E a qualidade de vida? Estudo dos efeitos de um programa holístico de reabilitação neuropsicológica

A reabilitação após lesão cerebral adquirida (LCA) pretende minimizar os impactos dos défices na funcionalidade do indivíduo promovendo a sua Qualidade de Vida (Qvd). O objetivo deste estudo é avaliar o impacto de um programa holístico de reabilitação neuropsicológica (PHRN) nas dimensões da Qvd. Participaram 20 sujeitos com LCA, média de idades 30 anos (DP=7.62). Os participantes foram avaliados pré e pós intervenção, com intervalo de seis meses utilizando-se o Quality Of Life after Brain Injury. Para a análise usamos o t-test amostras emparelhadas, com p corrigido para comparações múltiplas. Verifica-se uma avaliação mais satisfatória após a intervenção em todas as dimensões: total [t(19)=-4.59, p =0.001], social [t(19)=-1.29, p =0.001], emocional [t(19)=-1.85, p =0.001] e físico [t(19)=-1.778, p =0.01). Os resultados sugerem que o PHRN promove melhorias em todas as dimensões da Qdv.info:eu-repo/semantics/publishedVersio

Clinical manifestations and treatment of mucopolysaccharidosis type I patients in Latin America as compared with the rest of the world

Background Mucopolysaccharidosis I (MPS I) comprises a spectrum of clinical manifestations and is divided into three phenotypes reflecting clinical severity: Hurler, Hurler-Scheie, and Scheie syndromes. There may be important variations in clinical manifestations of this genetic disease in patients residing in different regions of the world.Methods Using data from the MPS I Registry (as of September 2009), we evaluated patients from Latin America (n=118) compared with patients from the rest of the world [ROW (n=727)].Results Phenotype distribution differed among patients in Latin America compared to ROW(Hurler 31 vs. 62%, Hurler-Scheie 36 vs. 21%, Scheie 10 vs. 11%, and unknown 22 vs. 6%). the frequency of certain symptoms, such as cardiac valve abnormalities, sleep impairment, and joint contractures, also differed between Latin America and ROW for some phenotypes. Median age at MPS I diagnosis was earlier in the ROW than Latin America for all phenotypes, and age at first treatment for Hurler and Hurler-Scheie patients was also earlier in the ROW. Hurler patients in Latin America showed a gap of 3.1 years between median ages of diagnosis and first treatment compared to only 0.5 years in the ROW. Treatment allocation in Latin America compared to ROW was as follows: enzyme replacement therapy (ERT) only, 80 vs. 45%; hematopoietic stem cell transplantation (HSCT) only, 0.9 vs. 27%; both ERT and HSCT, 0 vs. 16%; and neither treatment, 19 vs. 13%.Conclusion These data highlight important differences in MPS I patients between Latin America and ROW in terms of phenotypic distribution, clinical manifestations, and treatment practices.MPS I Registry team at Genzyme CorporationHosp Nacl Pediat JP Garrahan, Unidad Errores Congenitos Metab, Buenos Aires, DF, ArgentinaHosp Especialidades UMAE 25, Monterrey, MexicoGenzyme Corp, Latin Amer Grp, Registry Program, Rio de Janeiro, BrazilUniv Rosario, Fdn Univ Ciencias Salud, Bogota, ColombiaUniv Valparaiso, Fac Med, Neurol Infantil Programa Formac Neuropediat, Valparaiso, ChileUniv Chile, INTA, Lab Genet & Enfermedades Metab, Santiago, ChileUniversidade Federal de São Paulo, Ctr Referencia Erros Inatos Metab, São Paulo, BrazilGenzyme Corp, Latin Amer Grp, Compassionate Use Program, Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Ctr Referencia Erros Inatos Metab, São Paulo, BrazilWeb of Scienc

Impacto de um programa holístico de reabilitação neuropsicológica na qualidade de vida de pessoas com lesão cerebral adquirida

After acquired brain injury (ABI) rehabilitation aims to increase the individual’s functionality promoting their Quality of Life (QoL). The main goal of this study was to evaluate the impact of a holistic neuropsychological rehabilitation program (HNRP) on quality of life dimensions. Twenty subjects with ABI participated, mean age 30 years (SD=7.62).Participants were assessed pre and post intervention, with a six months interval, using Quality of Life After Brain Injury (QOLIBRI) A paired-sample t-test was conducted for data analyzes, with corrected p for multiple comparisons. Significant results after intervention were observed at all dimensions: total [t(19) = -4.59, p < .001], cognitive [t(19) = -2.31, p = .003], self [t(19) =-5.76, p < .001], DLAs (daily life activities) [t(19) = -3.18, p = .001], social [t(19) = -1.29, p = .001], emotional [t(19) = -1.85, p = .001], except on physical [t(19) = -1.78, p = .01]. Results suggest that intervention, after ABI, with the HNRP improves patients’ QoL.A reabilitação após lesão cerebral adquirida (LCA) pretende aumentar a funcionalidade do indivíduo, promovendo a sua Qualidade de Vida (QdV). O objetivo deste estudo é avaliar o impacto de um programa holístico de reabilitação neuropsicológica (PHRN) nas dimensões da QdV. Participaram 20 pessoas com LCA, média de idades 30 anos (DP = 7.62). Os participantes foram avaliados pré e pós intervenção, com intervalo de seis meses, utilizando-se o Quality of Life After Brain Injury (QOLIBRI). Para a análise usamos o t-test para amostras emparelhadas, com p corrigido para comparações múltiplas. Verificam-se resultados significativos após a intervenção em todas as dimensões: total [t(19) = -4.59, p < .001], cognitiva [t(19) = -2.31, p = .003], self [t(19) =-5.76, p < .001], AVD (atividades de vida diária) [t(19) = -3.18, p = .001], social [t(19) = -1.29, p = .001], emocional [t(19) = -1.85, p = .001], com exceção da dimensão físico [t(19) = -1.78, p = .01]. Os resultados sugerem que a intervenção com o PHRN promove a QdV das pessoas com LCA

Anti-SARS-CoV-2 inactivated vaccine in patients with ANCA-associated vasculitis: Immunogenicity, safety, antibody decay and the booster dose

Objective: To evaluate inactivated CoronaVac prime vaccination, antibody decay, booster dose, and safety in ANCA-Associated Vasculitis (AAV) patients. Methods: Fifty-three AAV patients and 106 Controls (CG) received CoronaVac on days: D0 (first dose), D28(second dose), and D210 (booster dose, 32 AAV: 32 CG). The primary outcome was immunogenicity after the second vaccine dose (day 69) assessed by Seroconversion Rates (SC) of anti-SARS-CoV-2 S1/S2 IgG and Neutralizing Antibodies (NAb). Secondary outcomes were safety, immunogenicity (D28/D240), 6-months antibody decay (D210) and the booster dose response (D240). Results: At D69 SC (65.1% vs. 96.8%, p = 0.0001), GMT (21.3 UA/mL vs. 67.7 UA/mL, p < 0.001) and NAb- positivity (53.7% vs. 80.6%, p = 0.001) were moderate but lower in naïve-AAV patients than CG. Patients without SC used more often IS (93.3% vs. 53.3%, p = 0.015), mycophenolate mofetil (20% vs. 0%, p = 0.037) and prednisone (60.0% vs. 28.6%, p = 0.057) than seroconverted. NAb negativity in AAV patients was associated with prednisone treatment (57.9% vs. 18.2%, p = 0.015) and IS (84.2% vs. 55.0%, p = 0.046). Logistic regression analysis models showed that only prednisone was associated with lower seroconversion (OR = 0.2, 0,95% CI 0.05‒0.86, p = 0.030) and with lower NAb positivity (OR = 0.2, 0,95% CI 0.05‒0.88, p = 0.034). After six months (D69‒D210) a decrease in IgG positivity occurred in 32 AAV patients (15.7%, p = 0.074) and 32 CG (18.7%, p = 0.041). For the NAb positivity, the 6-month decrease was not significant (p = 0.114) whereas a major reduction occurred for CG (p < 0.001). A booster dose (D240) resulted in an increment in IgG-positivity (21.9%, p = 0.023) and NAb-positivity (34.4%, p = 0.006) in AAV patients. No moderate/severe adverse events attributable to the vaccine were observed. Conclusion: This study provides novel data on the excellent safety and moderate immunogenicity of CoronaVac in AAV patients. A six-month mild antibody waning was observed with a good response to the booster dose, although levels remained lower than CG (CoronavRheum-NCT04754698)