The Determination of induction and differentiation in grape vines

The induction and differentiation of 8-year-old Alphonse Lavallee and Sultana grape vines were studied.Defoliation methods enabled us to determine the induction time in grape vines as in other fruit species.Induction and differentiation in the tested varieties were not connected with temporary growth cessation; on the contrary, process took place during the most intensive growth.A correlation was found between the number of leaves and induction period. 18-21 leaves above the examined buds were needed in bot-h varieties to complete the induction.The leaf area needed for induction in a bud of Sultana was lYe times larger than that needed for Alphonse. The efficiency of the leaves of Alphonse to induce differentiation was thus greater.The primordia ,development from induction to detection under the microscope (differentiation) was connected with a constant vegetative development. The time needed for this development was determined by the growth rate of the variety (18 days in Sultana, 14 days in Alphonse).The translocation of materials inducing differentiation from the base of the shoot upwar,ds has not been proved in our work.In Alphonse a lag period of two days was found for the differentiation of each bud along the cane

Analysis of water consumption of various grapevine cultivars

The efficiency of water use (transpiration ratio) was investigated in various cultivars of grapevine by gravimetric and gasometric methods. Results of gravimetric measurements showed positive correlation between water use efficiency and vigor. Regression lines of the transpiration ratio on the final dry weight differed significantly in their curves and their distance from the coordinate axes. Statistical analysis showed that the quantitative effect of vigor on the water use efficiency was smaller in the cultivar Muscat Hamburg than in Sultanina and Queen of the Vineyards. Covariance analysis showed that Sultanina was a less efficient water user than Queen of the Vineyards for plants with the same vigor. Gasometric methods did not reveal any significant differences between the cultivars, probably due to the elimination of interference and boundary layer resistance effect

Computational Physics on Graphics Processing Units

The use of graphics processing units for scientific computations is an emerging strategy that can significantly speed up various different algorithms. In this review, we discuss advances made in the field of computational physics, focusing on classical molecular dynamics, and on quantum simulations for electronic structure calculations using the density functional theory, wave function techniques, and quantum field theory.Comment: Proceedings of the 11th International Conference, PARA 2012, Helsinki, Finland, June 10-13, 201

Salmonella and tomatoes

Outbreak information linking fresh tomato fruit to illnesses is reviewed in this chapter. While tomato fruit appear to support substantial proliferation of certain serovars of Salmonella enterica, detection of this pathogen in tomato plants prior to harvest is rare, and reports of Salmonella existence in tomato fruit still attached to field-grown plants are virtually non-existent. The bacterium is sensitive to UV and can be outcompeted by the native phytomicrobiota, which may explain its absence in field-grown crops. However, the persistence of certain serovars in fields and ponds of certain production areas is noted. Together with evidence of bacteria becoming internalized in tomato fruit during crop development likely through natural apertures, the presence of S. enterica in and around production fields suggests that an unusual weather event could lead to Salmonella contamination of fruit prior to harvest. The bacterium appears physiologically adaptive toward proliferation in tomato fruit. Once inside tomatoes, Salmonella is capable of sensing the availability of nutrients and physiological state of the fruit and differentially regulates specific genes. However, because Salmonella is an efficient nutrient scavenger, removal of multiple metabolic and regulatory genes was required to reduce its fitness within the fruit. Plants do not appear to recognize human enterics as pathogens, and their defenses treat them as endophytes

Factors associated with diversity, quantity and zoonotic potential of ectoparasites on urban mice and voles

Wild rodents are important hosts for tick larvae but co-infestations with other mites and insects are largely neglected. Small rodents were trapped at four study sites in Berlin, Germany, to quantify their ectoparasite diversity. Host-specific, spatial and temporal occurrence of ectoparasites was determined to assess their influence on direct and indirect zoonotic risk due to mice and voles in an urban agglomeration. Rodent-associated arthropods were diverse, including 63 species observed on six host species with an overall prevalence of 99%. The tick Ixodes ricinus was the most prevalent species, found on 56% of the rodents. The trapping location clearly affected the presence of different rodent species and, therefore, the occurrence of particular host-specific parasites. In Berlin, fewer temporary and periodic parasite species as well as non-parasitic species (fleas, chiggers and nidicolous Gamasina) were detected than reported from rural areas. In addition, abundance of parasites with low host-specificity (ticks, fleas and chiggers) apparently decreased with increasing landscape fragmentation associated with a gradient of urbanisation. In contrast, stationary ectoparasites, closely adapted to the rodent host, such as the fur mites Myobiidae and Listrophoridae, were most abundant at the two urban sites. A direct zoonotic risk of infection for people may only be posed by Nosopsyllus fasciatus fleas, which were prevalent even in the city centre. More importantly, peridomestic rodents clearly supported the life cycle of ticks in the city as hosts for their subadult stages. In addition to trapping location, season, host species, body condition and host sex, infestation with fleas, gamasid Laelapidae mites and prostigmatic Myobiidae mites were associated with significantly altered abundance of I. ricinus larvae on mice and voles. Whether this is caused by predation, grooming behaviour or interaction with the host immune system is unclear. The present study constitutes a basis to identify interactions and vector function of rodent-associated arthropods and their potential impact on zoonotic diseases

Study on avifauna and species richness in Karanja-Sohol wildlife sanctuary, (MS) India

The present study was carried out during a bird race organized in Karanja-Sohol Wildlife sanctuary Karanja, district Washim Maharashtra, India. The objective of this study was to assess the avifauna of wildlife sanctuaries. Due to the geological and ecological complexity of the area, it has become imperative to conduct research on the region's avian diversity. It is challenging to conduct this assessment, as the wildlife sanctuary is divided into numerous patches by the state highway running through it. Sanctuary is surrounded by numerous villages and agricultural land, which creates human interference and livestock grazing. This checklist will serve as a baseline for further study, as there is no published checklist of this area. In the present investigation, we reported a total of 151 bird species during the exhaustive survey by the authors and accompanied volunteers during the census organized by the wildlife department. The observed species of birds belong to 55 families and 17 orders. They also recorded their residential and IUCN red data status

DNA sequence-specific recognition by a transcriptional regulator requires indirect readout of A-tracts

The bacteriophage Ø29 transcriptional regulator p4 binds to promoters of different intrinsic activities. The p4–DNA complex contains two identical protomers that make similar interactions with the target sequence 5′-AACTTTTT-15 bp-AAAATGTT-3′. To define how the various elements in the target sequence contribute to p4's affinity, we studied p4 binding to a series of mutated binding sites. The binding specificity depends critically on base pairs of the target sequence through both direct as well as indirect readout. There is only one specific contact between a base and an amino acid residue; other contacts take place with the phosphate backbone. Alteration of direct amino acid–base contacts, or mutation of non-contacted A·T base pairs at A-tracts abolished binding. We generated three 5 ns molecular dynamics (MD) simulations to investigate the basis for the p4–DNA complex specificity. Recognition is controlled by the protein and depends on DNA dynamic properties. MD results on protein–DNA contacts and the divergence of p4 affinity to modified binding sites reveal an inherent asymmetry, which is required for p4-specific binding and may be crucial for transcription regulation