This article is published in cooperation with the XIIIth International Terroir Congress November 17-18 2020, Adelaide, Australia. Guests editors: Cassandra Collins and Roberta De Bei

Currently, the greatest challenge for vine growers is to improve the yield and quality of grapes by minimizing costs and environmental impacts. This goal can be achieved through a better knowledge of vineyard spatial variability. Traditional platforms such as airborne, satellite and unmanned aerial vehicles (UAVs) solutions are useful investigation tools for vineyard site specific management. These remote sensing techniques are mainly exploited to get the Normalized Difference Vegetation Index (NDVI), which is useful for describing the morpho-vegetational characteristics of vineyards. This study was conducted in a vineyard in Tuscany (Italy) during the 2017, 2018 and 2019 seasons. Ground data were acquired to detect some agronomic variables such as yield (kg/vine), total soluble solids (TSS), and pruning weight (kg/vine). Remote sensed multispectral images acquired by UAV and Sentinel-2 (S2) satellite platform were used to assess the analysis of the vegetative variability. The UAV NDVI was extracted using both a mixed pixels approach (both vine and inter-row) and from pure canopy pixels. In addition to these UAV layers, the vine thickness was extracted. The aim of this study was to evaluate both classical Ordinary Least Square (OLS) and spatial statistical methods (Moran Index-MI and BILISA) to assess their performance in a multi-temporal comparison between satellite and ground data with UAV information. Good correlations were detected between S2 NDVI and UAV NDVI mixed pixels through both methods (R2 = 0.80 and MI = 0.75). Regarding ground data, UAV layers showed low and negative association with TSS (MI = - 0.34 was the lowest value) whereas better spatial autocorrelations with positive values were detected between UAV layers and both yield (MI ranged from 0.42 to 0.52) and pruning weight (MI ranged from 0.45 to 0.64). The spatial analysis made by MI and BILISA methodologies added more information to this study, clearly showing that both UAV and Sentinel-2 satellite allowed the vigour spatial variability within the vineyard to be detected correctly, overcoming the classical comparison methods by adding the spatial effect. MI and BILISA play a key role in identifying spatial patterns and could be successfully exploited by agricultural stakeholders

A Low-Cost and Unsupervised Image Recognition Methodology for Yield Estimation in a Vineyard

Yield prediction is a key factor to optimize vineyard management and achieve the desired grape quality. Classical yield estimation methods, which consist of manual sampling within the field on a limited number of plants before harvest, are time-consuming and frequently insufficient to obtain representative yield data. Non-invasive machine vision methods are therefore being investigated to assess and implement a rapid grape yield estimate tool. This study aimed at an automated estimation of yield in terms of cluster number and size from high resolution RGB images (20 MP) taken with a low-cost UAV platform in representative zones of the vigor variability within an experimental vineyard. The flight campaigns were conducted in different light conditions and canopy cover levels for 2017 and 2018 crop seasons. An unsupervised recognition algorithm was applied to derive cluster number and size, which was used for estimating yield per vine. The results related to the number of clusters detected in different conditions, and the weight estimation for each vigor zone are presented. The segmentation results in cluster detection showed a performance of over 85% in partially leaf removal and full ripe condition, and allowed grapevine yield to be estimated with more than 84% of accuracy several weeks before harvest. The application of innovative technologies in field-phenotyping such as UAV, high-resolution cameras and visual computing algorithms enabled a new methodology to assess yield, which can save time and provide an accurate estimate compared to the manual method

AgroShadow: A New Sentinel-2 Cloud Shadow Detection Tool for Precision Agriculture

Remote sensing for precision agriculture has been strongly fostered by the launches of the European Space Agency Sentinel-2 optical imaging constellation, enabling both academic and private services for redirecting farmers towards a more productive and sustainable management of the agroecosystems. As well as the freely and open access policy adopted by the European Space Agency (ESA), software and tools are also available for data processing and deeper analysis. Nowadays, a bottleneck in this valuable chain is represented by the difficulty in shadow identification of Sentinel-2 data that, for precision agriculture applications, results in a tedious problem. To overcome the issue, we present a simplified tool, AgroShadow, to gain full advantage from Sentinel-2 products and solve the trade-off between omission errors of Sen2Cor (the algorithm used by the ESA) and commission errors of MAJA (the algorithm used by Centre National d'Etudes Spatiales/Deutsches Zentrum für Luft- und Raumfahrt, CNES/DLR). AgroShadow was tested and compared against Sen2Cor and MAJA in 33 Sentinel 2A-B scenes, covering the whole of 2020 and in 18 different scenarios of the whole Italian country at farming scale. AgroShadow returned the lowest error and the highest accuracy and F-score, while precision, recall, specificity, and false positive rates were always similar to the best scores which alternately were returned by Sen2Cor or MAJA

Retrieving soil moisture in rainfed and irrigated fields using Sentinel-2 observations and a modified OPTRAM approach

Abstract Surface soil water content plays an important role in driving the exchange of latent and sensible heat between the atmosphere and land surface through transpiration and evaporation processes, regulating key physiological processes affecting plants growth. Given the high impact of water scarcity on yields, and of irrigated agriculture on the overall withdrawal rate of freshwater, it is important to define models that help to improve water resources management for agricultural purposes, and to optimize rainfed crop yield. Recent advances in satellite-based remote sensing have led to valuable solutions to estimate soil water content based on microwave or optical/thermal-infrared data. This study aims at improving soil water content estimation at high spatial and temporal resolution, by means of the Optical Trapezoid Model (OPTRAM) driven by Copernicus Sentinel-2 data. Two different model variations were considered, based on linear and nonlinear parameters constraints, and validated against in situ soil water content measurements made with time domain reflectometry (TDR) on irrigated maize in central Italy and on rainfed maize and pasture in northern Italy. For the first site the non-linear model shows a better correlation between measured and estimated soil water content values (r = 0.80) compared to the linear model (r = 0.73). In both cases the modeled soil moisture tends to overestimate the measured values at medium to high water content level, while both models underestimate soil moisture at low water content level. Estimated versus measured normalized surface soil water for rainfed pasture plots from nonlinear OPTRAM parametrized based on irrigated maize parameterization (SIM1), and site-specific parametrization for rainfed pasture (SIM2), indicate that both models (SIM1 and SIM2) are comparable for rotational grazing pasture (RMSEsim1 = 0.0581 vs. RMSEsim2 = 0.0485 cm3 cm-3) and the continuous grazing pasture (RMSEsim1 = 0.0485 vs. RMSEsim2 = 0.0602 cm3 cm-3), while for the rainfed maize plots SIM1 shows lower RMSE (average for all plots RMSE = 0.0542 cm3 cm-3) compared to the site-specific calibration model (SIM2 – average for all plots RMSE = 0.0645 cm3 cm-3). Finally, OPTRAM estimations are close to in situ measurement values while Surface Soil Moisture at 1 km (SSM1 km) tends to underestimate the measurements during maize crop growing season. Soil moisture retrieval from high-resolution Sentinel-2 optical images allows water stress conditions to be effectively mapped, supporting decision making in irrigation scheduling and other crop management

An Integrated Approach for the Monitoring of Brain and Autonomic Response of Children with Autism Spectrum Disorders during Treatment by Wearable Technologies

Autism Spectrum Disorders (ASD) are associated with physiological abnormalities, which are likely to contribute to the core symptoms of the condition. Wearable technologies can provide data in a semi-naturalistic setting, overcoming the limitations given by the constrained situations in which physiological signals are usually acquired. In this study an integrated system based on wearable technologies for the acquisition and analysis of neurophysiological and autonomic parameters during treatment is proposed and an application on five children with ASD is presented. Signals were acquired during a therapeutic session based on an imitation protocol in ASD children. Data were analyzed with the aim of extracting quantitative EEG (QEEG) features from EEG signals as well as heart rate and heart rate variability (HRV) from ECG. The system allowed evidencing changes in neurophysiological and autonomic response from the state of disengagement to the state of engagement of the children, evidencing a cognitive involvement in the children in the tasks proposed. The high grade of acceptability of the monitoring platform is promising for further development and implementation of the tool. In particular if the results of this feasibility study would be confirmed in a larger sample of subjects, the system proposed could be adopted in more naturalistic paradigms that allow real world stimuli to be incorporated into EEG/psychophysiological studies for the monitoring of the effect of the treatment and for the implementation of more individualized therapeutic programs

A machine-learning based bio-psycho-social model for the prediction of non-obstructive and obstructive coronary artery disease

Background: Mechanisms of myocardial ischemia in obstructive and non-obstructive coronary artery disease (CAD), and the interplay between clinical, functional, biological and psycho-social features, are still far to be fully elucidated. Objectives: To develop a machine-learning (ML) model for the supervised prediction of obstructive versus non-obstructive CAD. Methods: From the EVA study, we analysed adults hospitalized for IHD undergoing conventional coronary angiography (CCA). Non-obstructive CAD was defined by a stenosis < 50% in one or more vessels. Baseline clinical and psycho-socio-cultural characteristics were used for computing a Rockwood and Mitnitski frailty index, and a gender score according to GENESIS-PRAXY methodology. Serum concentration of inflammatory cytokines was measured with a multiplex flow cytometry assay. Through an XGBoost classifier combined with an explainable artificial intelligence tool (SHAP), we identified the most influential features in discriminating obstructive versus non-obstructive CAD. Results: Among the overall EVA cohort (n = 509), 311 individuals (mean age 67 ± 11 years, 38% females; 67% obstructive CAD) with complete data were analysed. The ML-based model (83% accuracy and 87% precision) showed that while obstructive CAD was associated with higher frailty index, older age and a cytokine signature characterized by IL-1β, IL-12p70 and IL-33, non-obstructive CAD was associated with a higher gender score (i.e., social characteristics traditionally ascribed to women) and with a cytokine signature characterized by IL-18, IL-8, IL-23. Conclusions: Integrating clinical, biological, and psycho-social features, we have optimized a sex- and gender-unbiased model that discriminates obstructive and non-obstructive CAD. Further mechanistic studies will shed light on the biological plausibility of these associations. Clinical trial registration: NCT02737982

Clinical features and outcomes of elderly hospitalised patients with chronic obstructive pulmonary disease, heart failure or both

Background and objective: Chronic obstructive pulmonary disease (COPD) and heart failure (HF) mutually increase the risk of being present in the same patient, especially if older. Whether or not this coexistence may be associated with a worse prognosis is debated. Therefore, employing data derived from the REPOSI register, we evaluated the clinical features and outcomes in a population of elderly patients admitted to internal medicine wards and having COPD, HF or COPD + HF. Methods: We measured socio-demographic and anthropometric characteristics, severity and prevalence of comorbidities, clinical and laboratory features during hospitalization, mood disorders, functional independence, drug prescriptions and discharge destination. The primary study outcome was the risk of death. Results: We considered 2,343 elderly hospitalized patients (median age 81 years), of whom 1,154 (49%) had COPD, 813 (35%) HF, and 376 (16%) COPD + HF. Patients with COPD + HF had different characteristics than those with COPD or HF, such as a higher prevalence of previous hospitalizations, comorbidities (especially chronic kidney disease), higher respiratory rate at admission and number of prescribed drugs. Patients with COPD + HF (hazard ratio HR 1.74, 95% confidence intervals CI 1.16-2.61) and patients with dementia (HR 1.75, 95% CI 1.06-2.90) had a higher risk of death at one year. The Kaplan-Meier curves showed a higher mortality risk in the group of patients with COPD + HF for all causes (p = 0.010), respiratory causes (p = 0.006), cardiovascular causes (p = 0.046) and respiratory plus cardiovascular causes (p = 0.009). Conclusion: In this real-life cohort of hospitalized elderly patients, the coexistence of COPD and HF significantly worsened prognosis at one year. This finding may help to better define the care needs of this population

Beta-Blocker Use in Older Hospitalized Patients Affected by Heart Failure and Chronic Obstructive Pulmonary Disease: An Italian Survey From the REPOSI Register

Beta (β)-blockers (BB) are useful in reducing morbidity and mortality in patients with heart failure (HF) and concomitant chronic obstructive pulmonary disease (COPD). Nevertheless, the use of BBs could induce bronchoconstriction due to β2-blockade. For this reason, both the ESC and GOLD guidelines strongly suggest the use of selective β1-BB in patients with HF and COPD. However, low adherence to guidelines was observed in multiple clinical settings. The aim of the study was to investigate the BBs use in older patients affected by HF and COPD, recorded in the REPOSI register. Of 942 patients affected by HF, 47.1% were treated with BBs. The use of BBs was significantly lower in patients with HF and COPD than in patients affected by HF alone, both at admission and at discharge (admission, 36.9% vs. 51.3%; discharge, 38.0% vs. 51.7%). In addition, no further BB users were found at discharge. The probability to being treated with a BB was significantly lower in patients with HF also affected by COPD (adj. OR, 95% CI: 0.50, 0.37-0.67), while the diagnosis of COPD was not associated with the choice of selective β1-BB (adj. OR, 95% CI: 1.33, 0.76-2.34). Despite clear recommendations by clinical guidelines, a significant underuse of BBs was also observed after hospital discharge. In COPD affected patients, physicians unreasonably reject BBs use, rather than choosing a β1-BB. The expected improvement of the BB prescriptions after hospitalization was not observed. A multidisciplinary approach among hospital physicians, general practitioners, and pharmacologists should be carried out for better drug management and adherence to guideline recommendations

Clinical features and outcomes of elderly hospitalised patients with chronic obstructive pulmonary disease, heart failure or both

Background and objective: Chronic obstructive pulmonary disease (COPD) and heart failure (HF) mutually increase the risk of being present in the same patient, especially if older. Whether or not this coexistence may be associated with a worse prognosis is debated. Therefore, employing data derived from the REPOSI register, we evaluated the clinical features and outcomes in a population of elderly patients admitted to internal medicine wards and having COPD, HF or COPD + HF. Methods: We measured socio-demographic and anthropometric characteristics, severity and prevalence of comorbidities, clinical and laboratory features during hospitalization, mood disorders, functional independence, drug prescriptions and discharge destination. The primary study outcome was the risk of death. Results: We considered 2,343 elderly hospitalized patients (median age 81 years), of whom 1,154 (49%) had COPD, 813 (35%) HF, and 376 (16%) COPD + HF. Patients with COPD + HF had different characteristics than those with COPD or HF, such as a higher prevalence of previous hospitalizations, comorbidities (especially chronic kidney disease), higher respiratory rate at admission and number of prescribed drugs. Patients with COPD + HF (hazard ratio HR 1.74, 95% confidence intervals CI 1.16-2.61) and patients with dementia (HR 1.75, 95% CI 1.06-2.90) had a higher risk of death at one year. The Kaplan-Meier curves showed a higher mortality risk in the group of patients with COPD + HF for all causes (p = 0.010), respiratory causes (p = 0.006), cardiovascular causes (p = 0.046) and respiratory plus cardiovascular causes (p = 0.009). Conclusion: In this real-life cohort of hospitalized elderly patients, the coexistence of COPD and HF significantly worsened prognosis at one year. This finding may help to better define the care needs of this population