Wissenschaftliche Sammlung UNESCO-Biosphärenreservat Rhön – ein Modell für die Biosphärenreservate in Deutschland : Überlegungen zur Übertragbarkeit

Die Wissenschaftliche Sammlung UNESCO-Biosphärenreservat Rhön wird auf der Grundlage eines detaillierten Förderantrags seit dem Jahr 2004 für einen Zeitraum von etwa drei Jahren aus dem Innovationsbudget des Hessischen Ministeriums für Wissenschaft und Kunst als Projekt gefördert. Ein im Projektantrag bereits formulierter wichtiger Aspekt in der Zielsetzung des Vorhabens ist es, am Ende der Projektlaufzeit die Erkenntnisse, Erfahrungen und Verläufe als modellhaft im Hinblick auf die Möglichkeiten einer Übertragbarkeit auf andere Biosphärenreservate zur Diskussion zu stellen. Mit diesem Beitrag wollen wir eine solche Debatte anregen und wünschen uns entsprechende Resonanz

The genome of herpesvirus saimiri C488 which is capable of transforming human T cells

AbstractHerpesvirus saimiri (HVS), the rhadinovirus prototype, is apathogenic in the persistently infected natural host, the squirrel monkey, but causes acute T cell leukemia in other New World primate species. In contrast to subgroups A and B, only strains of HVS subgroup C such as C488 are capable of transforming primary human T cells to stable antigen-independent growth in culture. Here, we report the complete 155-kb genome sequence of the transformation-competent HVS strain C488. The A+T-rich unique L-DNA of 113,027 bp encodes at least 77 open reading frames and 5 URNAs. In addition to the viral oncogenes stp and tip, only a few genes including the transactivator orf50 and the glycoprotein orf51 are highly divergent. In a series of new primary HVS isolates, the subgroup-specific divergence of the orf50/orf51 alleles was studied. In these new isolates, the orf50/orf51 alleles of the respective subgroup segregate with the stp and/or tip oncogene alleles, which are essential for transformation

Evaluating the practicability of commercial food-scanners for non-destructive quality assessment of tomato fruit

The assessment  of  tomato  fruit  quality  depends  on  a  variety  of  extrinsic and intrinsic quality parameters such as color, firmness and sugar content. Conventional measurement methods of these quality parameters  are  time  consuming,  require  various  measurement  de-vices,  and  in  case  of  intrinsic  quality,  involve  destructive  measurements. Latest research focused on the non-destructive determination of these parameters by using spectroscopic measurements. The goal of  this  study  was  to  evaluate  the  capability  of  three  commercially  available  portable  and  miniaturized  VIS /NIR spectrometers, so called food-scanners, in predicting various tomato quality attributes in a non-destructive way. Additionally, this study evaluated the software provided by manufacturers for building of prediction models by comparing the results derived from those software tools to state-of-the-art software for multivariate analysis. Evaluation of food-scanner spectra resulted in prediction models of high accuracy (r² > 0.90) for tomato fruit firmness, dry matter, total soluble solids and color values L*, a* and h°. Prediction models computed with manufacturer’s soft-ware showed similar accuracy to those derived from state-of-the-art evaluation software. Results of this study illustrate the great potential of  commercial  food-scanners  for  non-destructive  quality  measurement.  Further  important  features  of  food-scanners  with  respect  to  the application along the fresh produce supply chain are addressed

Indoor-Vertical Farming: Consistent further development of protected cultivation

Der Klimawandel und die zunehmende weltweite Urbanisierung treiben den Ausbau von geschützten Produktionssystemen an. Allerdings bleibt die Abhängigkeit gegenüber äußeren Witterungsbedingungen bestehen. Indoor Vertical Farming hingegen verfolgt die vollständige Unabhängigkeit von äußeren Bedingungen, mit dem Ziel einer hochgenauen Steuerung aller Kulturparameter. Gerade bezogen auf den voranschreitenden Klimawandel und die Notwendigkeit zur ressourcenschonenden Produktion ergeben sich deutliche Vorteile durch die ganzjährige, vom Standort unabhängige Produktion von Pflanzen und Rohstoffen. Die Komplexität in der optimalen Vernetzung der Systeme Pflanze-Technik bietet intensive Entwicklungsmöglichkeiten für Digitalisierung und interdisziplinäre Zusammenarbeit.Climate change and increasing global urbanization accelerate the expansion of protected cultivation systems. However, certain dependences to external weather conditions remain even in modern greenhouses. Indoor vertical farming, on the other hand, pursues complete independence from external weather conditions with the aim for highly accurate control of all crop parameters. Particularly with regard to the advancing climate change and the need for sustainable resource consumption, there are clear advantages due to the year-round and independent cultivation of plants and raw materials under optimal conditions. The complexity in the optimal networking of the plant-technology systems offers intensive development opportunities for digitization and interdisciplinary collaboration

An Equine Model for Vaccination against a Hepacivirus: Insights into Host Responses to E2 Recombinant Protein Vaccination and Subsequent Equine Hepacivirus Inoculation

Equine hepacivirus (EqHV) is the closest known genetic homologue of hepatitis C virus. An effective prophylactic vaccine is currently not available for either of these hepaciviruses. The equine as potential surrogate model for hepacivirus vaccine studies was investigated, while equine host responses following vaccination with EqHV E2 recombinant protein and subsequent EqHV inoculation were elucidated. Four ponies received prime and booster vaccinations (recombinant protein, adjuvant) four weeks apart (day −55 and −27). Two control ponies received adjuvant only. Ponies were inoculated with EqHV RNA-positive plasma on day 0. Blood samples and liver biopsies were collected over 26 weeks (day −70 to +112). Serum analyses included detection of EqHV RNA, isotypes of E2-specific immunoglobulin G (IgG), nonstructural protein 3-specific IgG, haematology, serum biochemistry, and metabolomics. Liver tissue analyses included EqHV RNA detection, RNA sequencing, histopathology, immunohistochemistry, and fluorescent in situ hybridization. Al-though vaccination did not result in complete protective immunity against experimental EqHV inoculation, the majority of vaccinated ponies cleared the serum EqHV RNA earlier than the control ponies. The majority of vaccinated ponies appeared to recover from the EqHV-associated liver insult earlier than the control ponies. The equine model shows promise as a surrogate model for future hepacivirus vaccine research

An Equine Model for Vaccination against a Hepacivirus: Insights into Host Responses to E2 Recombinant Protein Vaccination and Subsequent Equine Hepacivirus Inoculation

Equine hepacivirus (EqHV) is the closest known genetic homologue of hepatitis C virus. An effective prophylactic vaccine is currently not available for either of these hepaciviruses. The equine as potential surrogate model for hepacivirus vaccine studies was investigated, while equine host responses following vaccination with EqHV E2 recombinant protein and subsequent EqHV inoculation were elucidated. Four ponies received prime and booster vaccinations (recombinant protein, adjuvant) four weeks apart (day −55 and −27). Two control ponies received adjuvant only. Ponies were inoculated with EqHV RNA-positive plasma on day 0. Blood samples and liver biopsies were collected over 26 weeks (day −70 to +112). Serum analyses included detection of EqHV RNA, isotypes of E2-specific immunoglobulin G (IgG), nonstructural protein 3-specific IgG, haematology, serum biochemistry, and metabolomics. Liver tissue analyses included EqHV RNA detection, RNA sequencing, histopathology, immunohistochemistry, and fluorescent in situ hybridization. Al-though vaccination did not result in complete protective immunity against experimental EqHV inoculation, the majority of vaccinated ponies cleared the serum EqHV RNA earlier than the control ponies. The majority of vaccinated ponies appeared to recover from the EqHV-associated liver insult earlier than the control ponies. The equine model shows promise as a surrogate model for future hepacivirus vaccine research

Genome Sequencing of SHH Medulloblastoma Predicts Genotype-Related Response to Smoothened Inhibition

SummarySmoothened (SMO) inhibitors recently entered clinical trials for sonic-hedgehog-driven medulloblastoma (SHH-MB). Clinical response is highly variable. To understand the mechanism(s) of primary resistance and identify pathways cooperating with aberrant SHH signaling, we sequenced and profiled a large cohort of SHH-MBs (n = 133). SHH pathway mutations involved PTCH1 (across all age groups), SUFU (infants, including germline), and SMO (adults). Children >3 years old harbored an excess of downstream MYCN and GLI2 amplifications and frequent TP53 mutations, often in the germline, all of which were rare in infants and adults. Functional assays in different SHH-MB xenograft models demonstrated that SHH-MBs harboring a PTCH1 mutation were responsive to SMO inhibition, whereas tumors harboring an SUFU mutation or MYCN amplification were primarily resistant

The Maunder minimum (1645-1715) was indeed a grand minimum: a reassessment of multiple datasets

Aims. Although the time of the Maunder minimum (1645–1715) is widely known as a period of extremely low solar activity, it is still being debated whether solar activity during that period might have been moderate or even higher than the current solar cycle (number 24). We have revisited all existing evidence and datasets, both direct and indirect, to assess the level of solar activity during the Maunder minimum. Methods. We discuss the East Asian naked-eye sunspot observations, the telescopic solar observations, the fraction of sunspot active days, the latitudinal extent of sunspot positions, auroral sightings at high latitudes, cosmogenic radionuclide data as well as solar eclipse observations for that period. We also consider peculiar features of the Sun (very strong hemispheric asymmetry of the sunspot location, unusual differential rotation and the lack of the K-corona) that imply a special mode of solar activity during the Maunder minimum. Results. The level of solar activity during the Maunder minimum is reassessed on the basis of all available datasets. Conclusions. We conclude that solar activity was indeed at an exceptionally low level during the Maunder minimum. Although the exact level is still unclear, it was definitely lower than during the Dalton minimum of around 1800 and significantly below that of the current solar cycle #24. Claims of a moderate-to-high level of solar activity during the Maunder minimum are rejected with a high confidence level

Uncovering the Molecular Machinery of the Human Spindle—An Integration of Wet and Dry Systems Biology

The mitotic spindle is an essential molecular machine involved in cell division, whose composition has been studied extensively by detailed cellular biology, high-throughput proteomics, and RNA interference experiments. However, because of its dynamic organization and complex regulation it is difficult to obtain a complete description of its molecular composition. We have implemented an integrated computational approach to characterize novel human spindle components and have analysed in detail the individual candidates predicted to be spindle proteins, as well as the network of predicted relations connecting known and putative spindle proteins. The subsequent experimental validation of a number of predicted novel proteins confirmed not only their association with the spindle apparatus but also their role in mitosis. We found that 75% of our tested proteins are localizing to the spindle apparatus compared to a success rate of 35% when expert knowledge alone was used. We compare our results to the previously published MitoCheck study and see that our approach does validate some findings by this consortium. Further, we predict so-called “hidden spindle hub”, proteins whose network of interactions is still poorly characterised by experimental means and which are thought to influence the functionality of the mitotic spindle on a large scale. Our analyses suggest that we are still far from knowing the complete repertoire of functionally important components of the human spindle network. Combining integrated bio-computational approaches and single gene experimental follow-ups could be key to exploring the still hidden regions of the human spindle system