Approximation spectrale de matrices issues d opérateurs discrétisés

Cette thèse considère la solution numérique d'un problème aux valeurs propres de grandes dimensions, dans lequel l'opérateur est dérivé d'un problème de transfert radiatif. Ainsi, cette thèse étudie l'utilisation de matrices hiérarchiques, une représentation efficace de tableaux, très intéressante pour une utilisation avec des problèmes de grandes dimensions. Les matrices sont des représentations hiérarchiques de structures de données efficaces pour les matrices denses, l'idée de base étant la division d'une matrice en une hiérarchie de blocs et l approximation de certains blocs par une matrice de petite caractéristique. Son utilisation permet de diminuer la mémoire nécessaire tout en réduisant les coûts informatiques. L'application de l'utilisation de matrices hiérarchique est analysée dans le contexte de la solution numérique d'un problème aux valeurs propres de grandes dimensions résultant de la discrétisation d'un opérateur intégral. L'opérateur est de convolution et est défini par la première fonction exponentielle intégrale, donc faiblement singulière. Pour le calcul informatique, nous avons accès à HLIB (Hierarchical matrices LIBrary) qui fournit des routines pour la construction de la structure hiérarchique des matrices et des algorithmes pour les opérations approximative avec ces matrices. Nous incorporons certaines routines comme la multiplication matrice-vecteur ou la decomposition LU, en SLEPc (Hierarchical matrices LIBrary) pour explorer les algorithmes existants afin de résoudre les problèmes de valeur propre. Nous développons aussi des expressions analytiques pour l'approximation des noyaux dégénérés utilisés dans la thèse et déduire ainsi les limites supérieures d'erreur pour ces approximations. Les résultats numériques obtenus avec d'autres techniques pour résoudre le problème en question sont utilisés pour la comparaison avec ceux obtenus avec la nouvelle technique, illustrant l'efficacité de ce dernierIn this thesis, we consider the numerical solution of a large eigenvalue problem in which the integral operator comes from a radiative transfer problem. It is considered the use of hierarchical matrices, an efficient data-sparse representation of matrices, especially useful for large dimensional problems. It consists on low-rank subblocks leading to low memory requirements as well as cheap computational costs. We discuss the use of the hierarchical matrix technique in the numerical solution of a large scale eigenvalue problem arising from a finite rank discretization of an integral operator. The operator is of convolution type, it is defined through the first exponential-integral function and hence it is weakly singular. We access HLIB (Hierarchical matrices LIBrary) that provides, among others, routines for the construction of hierarchical matrix structures and arithmetic algorithms to perform approximative matrix operations. Moreover, it is incorporated the matrix-vector multiply routines from HLIB, as well as LU factorization for preconditioning, into SLEPc (Scalable Library for Eigenvalue Problem Computations) in order to exploit the available algorithms to solve eigenvalue problems. It is also developed analytical expressions for the approximate degenerate kernels and deducted error upper bounds for these approximations. The numerical results obtained with other approaches to solve the problem are used to compare with the ones obtained with this technique, illustrating the efficiency of the techniques developed and implemented in this workST ETIENNE-Bib. électronique (422189901) / SudocSudocFranceF

New insights into a bacterial metabolic and detoxifying association responsible for the mineralization of the thiocarbamate herbicide molinate

A novel pathway of molinate mineralization promoted by a defined mixed culture composed of five bacteria (named ON1 to ON5) was proposed previously. Evidence was obtained of a metabolic association between Gulosibacter molinativorax ON4T, capable of molinate breakdown, and the remaining bacteria. In the present study, the role of each isolate in that metabolic association was further explored and the possible synergistic effect of all the bacterial isolates for the stability of the mixed culture is discussed. The cleavage of the molinate thioester bond, whether occurring under aerobic or anaerobic conditions, releases ethanethiol (S-ethyl moiety) and an azepane moiety derivative, identified as azepane-1-carboxylic acid. This azepane moiety is degraded, in the presence of oxygen, by Pseudomonas strains ON1 and ON3 and G. molinativorax ON4T. Ethanethiol, which inhibits G. molinativorax ON4T, is consumed by Pseudomonas strain ON1 and Stenotrophomonas maltophilia ON2. Although a two-member mixed culture of G. molinativorax ON4T and Pseudomonas strain ON1 was able to promote the aerobic mineralization of molinate, after 20 successive transfers of the five-member mixed culture in mineral medium with molinate, none of these isolates were lost. The results obtained indicate that the whole mixed culture may have a higher fitness than the two-member culture, even when the basic degradative and cross-protection functions are assured

Photocatalytic ozonation of urban wastewater and surface water using immobilized TiO2 with LEDs: Micropollutants, antibiotic resistance genes and estrogenic activity

Photocatalytic ozonation was employed for the first time in continuous mode with TiO2-coated glass Raschig rings and light emitting diodes (LEDs) to treat urban wastewater as well as surface water collected from the supply area of a drinking water treatment plant (DWTP). Different levels of contamination and types of contaminants were considered in this work, including chemical priority substances (PSs) and contaminants of emerging concern (CECs), as well as potential human opportunistic antibiotic resistant bacteria and their genes (ARB&ARG). Photocatalytic ozonation was more effective than single ozonation (or even than TiO2 catalytic ozonation) in the degradation of typical reaction by-products (such as oxalic acid), and more effective than photocatalysis to remove the parent micropollutants determined in urban wastewater. In fact, only fluoxetine, clarithromycin, erythromycin and 17-alpha-ethinylestradiol (EE2) were detected after photocatalytic ozonation, by using solid-phase extraction (SPE) pre-concentration and LC-MS/MS analysis. In surface water, this treatment allowed the removal of all determined micropollutants to levels below the limit of detection (0.01-0.20 ng L(-1)). The efficiency of this process was then assessed based on the capacity to remove different groups of cultivable microorganisms and housekeeping (16S rRNA) and antibiotic resistance or related genes (intI1, blaTEM, qnrS, sul1). Photocatalytic ozonation was observed to efficiently remove microorganisms and ARGs. Although after storage total heterotrophic and ARB (to ciprofloxacin, gentamicin, meropenem), fungi, and the genes 16S rRNA and intI1, increased to values close to the pre-treatment levels, the ARGs (blaTEM, qnrS and sul1) were reduced to levels below/close to the quantification limit even after 3-days storage of treated surface water or wastewater. Yeast estrogen screen (YES), thiazolyl blue tetrazolium reduction (MTT) and lactate dehydrogenase (LDH) assays were also performed before and after photocatalytic ozonation to evaluate the potential estrogenic activity, the cellular metabolic activity and the cell viability. Compounds with estrogenic effects and significant differences concerning cell viability were not observed in any case. A slight cytotoxicity was only detected for Caco-2 and hCMEC/D3 cell lines after treatment of the urban wastewater, but not for L929 fibroblasts.info:eu-repo/semantics/acceptedVersio

Chronic kidney patients in hemodialysis: a study on the mode of psychosocial theory of Roy

Objective: To identify the adaptive problems of the psychosocial mode of the Roy model in chronic kidney patients on hemodialysis. Methods: Cross-sectional study with 178 patients from a dialysis center in the northeast of Brazil. Results: Three adaptive problems were identified, two of the self-concept: sexual dysfunction and low self-esteem; and one of the paper performance mode: paper failure. CONCLUSIONS: Adaptive flaws in the role, sexual dysfunction and low self-esteem refer to the presence of a conical disease and an invasive treatment, since these conditions directly affect the individual's lifestyle

Population genomics of Bombus terrestris reveals high but unstructured genetic diversity in a potential glacial refugium

Ongoing climate change is expected to cause an increase in temperature and a reduction of precipitation levels in the Mediterranean region, which might cause changes in many species distributions. These effects negatively influence species gene pools, decreasing genetic variability and adaptive potential. Here, we use mitochondrial DNA and RADseq to analyse population genetic structure and genetic diversity of the bumblebee species Bombus terrestris (subspecies Bombus terrestris lusitanicus), in the Iberian Peninsula. Although this subspecies shows a panmictic pattern of population structure across Iberia and beyond, we found differentiation between subspecies B. t. lusitanicus and B. t. africanus, probably caused by the existence of barriers to gene flow between Iberia and North Africa. Furthermore, the results revealed that the Iberian Peninsula harbours a large fraction of B. terrestris intraspecific genetic variation, with the highest number of mitochondrial haplotypes found when compared with any other region in Europe studied so far, suggesting a potential role for the Iberian Peninsula as a glacial refugium. Our findings strengthen the idea that Iberia is a very important source of diversity for the global genetic pool of this species, because rare alleles might play a role in population resilience against human- or climate-mediated changes.info:eu-repo/semantics/publishedVersio

Vivência do profissional fisioterapeuta no diagnóstico da hanseníase: um relato de experiência

Objective: consisted of understanding the view of the professional physiotherapist in the diagnosis of Hansen’s disease through dermatoneurological evaluation. Method: the study is descriptive, observational, of a qualitative character developed in a Health Unit in Belém / PA. The experience was carried out by the students of the Physiotherapy course at a State University of Pará, during the months of September and October 2017. A technical visit was made in order to get to know the Basic Health Unit, as well as the multidisciplinary work provided to the patient leprosy, emphasizing the role of the professional physiotherapist in the diagnosis of leprosy. Results and impacts: the main aspects highlighted in the interview were: quantity of positives to Hansen’s basil, perspective of cure, attendance in treatment, impacts on the level of experience as a physical therapist, among others. The district of Marambaira had a more expressive notification compared to the other neighborhoods, in 2016, of 27 positive cases, according to the Brazilian Institute of Research and Mapping and Information Technology at the Service of SUS. Conclusion: the experience of the physical therapist in the diagnosis of leprosy, expanded the knowledge of academics through information about guidelines and prevention of Hansen’s disease. Therefore, further studies on the interaction of health professionals, their experiences, with undergraduate students are fundamental.Objetivo: consistiu em compreender a visão do profissional fisioterapeuta no diagnóstico da Hanseníase por meio da avaliação dermatoneurológica. Método: o estudo é descritivo, observacional, de caráter qualitativo desenvolvido em uma Unidade de Saúde de Belém/PA. A vivência foi efetivada pelos acadêmicos do curso de Fisioterapia de uma Universidade Estadual do Pará, durante os meses de setembro e outubro de 2017. Foi realizada uma visita técnica a fim de conhecer a Unidade Básica de Saúde, bem como a atuação multidisciplinar prestada ao paciente hanseniano, enfatizando o papel do profissional fisioterapeuta no diagnóstico da Hanseníase. Resultados e impactos: os principais aspectos destacados na entrevista foram: quantidade de positivos ao basilo de Hansen, perspectiva de cura, assiduidade no tratamento, impactos a nível de vivência como profissional fisioterapeuta dentre outros. O bairro da Marambaira teve uma notificação mais expressiva comparado aos demais bairros, no ano de 2016, de 27 casos positivos, de acorod com o Instituto Brasileiro de Pesquisa e Mapeamento e Tecnologia da Informação a Serviço do SUS. Conclusão: a vivência do profissional fisioterapeuta no diagnóstico da hanseníase, ampliou o conhecimento dos acadêmicos por meio de informações acerca de orientações e prevenções da Hanseníase. Sendo assim, fundamental mais estudos sobre a interação dos profissionais de saúde, suas vivências, com os alunos de graduação.