55 research outputs found

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    On the occurrence and crop damage of wheat blossom midges (Contarinia tritici (Kby.) and Sitodiplosis mosellana (Geh.) in Saxony-Anhalt

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    Zu den ÄhrenschĂ€dlingen am Winterweizen zĂ€hlen die Gelbe WeizengallmĂŒcke (C. tritici (Kirby)) und die Orangerote WeizengallmĂŒcke (S. mosellana (GĂ©hin)) (Holland & al., 1996). In AbhĂ€ngigkeit vom Befallsstadium der WeizenĂ€hre und der Biologie der Erreger sind folgende Schadsymptome bekannt: Verminderung der Kornzahl pro Ähre (C. tritici) bzw. geschrumpfte oder missgebildete Körner (S. mosellana) (Mölck, 2006). Der Saatgutwert wird beeintrĂ€chtigt und die BackqualitĂ€t beeinflusst (Miller & Halton, 1961). Hinsichtlich Prognose und Überwachung des Auftretens von WeizengallmĂŒcken gab es bisher keine praxistauglichen methodischen AnsĂ€tze im Bundesland Sachsen-Anhalt. Hinzu kommt, dass der derzeit verstĂ€rkt praktizierte pfluglose Anbau von Weizen nach Weizen ein höheres Befallsrisiko erwarten lĂ€sst. Mit der Identifikation von Sexualpheromonen, die als hoch attraktiv fĂŒr MĂ€nnchen von S. mosellana gelten (Gries & al., 2000), eröffnen sich nun neue Prognosemöglichkeiten. Die effektive Nutzung von Pheromonfallen zum Monitoring von GallmĂŒcken könnte somit ein erster Baustein zur Erarbeitung praxistauglicher Richtwerte darstellen. In einer zweijĂ€hrigen Testphase wurde in Zusammenarbeit mit der Landesanstalt fĂŒr Landwirtschaft, Forsten und Gartenbau geprĂŒft, ob die Pheromonfallen eine Gradationswahrscheinlichkeit der ProblemschĂ€dlinge, insbesondere in Risikofruchtfolgen signalisieren können. Die Ergebnisse sowie Daten zu weiteren ÜberwachungsansĂ€tzen werden prĂ€sentiert.Lemon wheat blossom midges (Contarinia tritici) and Orange wheat blossom midges (Sitodiplosis mosellana) (Diptera: Cecidomyiidae) belong to the most prominent insect pests in winter wheat (Holland & al., 1996). However, no practical method exists to predict or monitor the impact of these insect pests. There is also a lack of recent research on the issue particulary for Central Germany (older studies include: LĂŒbke & Wetzel, 1984, Volkmar & 1989). Consequently, this study attempts to provide new data on the occurrence, monitoring and crop damage of wheat blossom midges. It also focuses on the impact of changing agricultural conditions such as wheat-to-wheat crop rotation, reduced soil tillage or different crop variants. A systematic survey of midge occurrence and crop damage in a wheat-to-wheat crop rotation was carried out at a research field (variants: Elvis, Altos in 2005) and conventional winter wheat fields in 2006 (variant Tommi). S. mosellana were monitored by means of pheromone traps. In 2006, white traps were tested as an alternative method to collect data on S. mosellana and C. tritici. Flight activity of adult midges was monitored on 13 different dates (Tab. 1). Crop damage was evaluated on 8 dates (Tab. 2). A total of 50 spikes per variant and date were randomly selected for microscopical examination. The analysis included parameters such as number of larvae per infested seed, number of corn thrips (larvae and adults) per spike or number of infested seeds per spike. In 2005, the activity of adult S. mosellana reached its peak in the phenological growth stage of full flowering (BBCH 65-69). The activity density was higher in the Elvis variant with 260 midges per trap (monitoring until mid-flowering) as compared to the Altos variant with 89 midges per trap (monitoring until the end of flowering period). In 2006, the activity of adult S. mosellana reached its peak in BBCH 51 (beginning of heading) with 246 midges per trap (Tommi variant). In 2005 the highest abundance of larvae per ear was established for BBCH 75 with an average of 2.1 (Altos) and 1.6 (Elvis). In 2006 these numbers were significantly higher. In the Tommi variant, an average of 14.3 larvae per ear was reached during BBCH 70-73. The ratio of midge-damaged kernels was evaluated in 2005 on BBCH 80-85 with 6.3 % (Altos) and 4.4 % (Elvis), while in 2006 a ratio of 23.5 % was reached in the Tommi variant. The results suggest that the intensity of crop damage depends on the correlation of two factors. The closer the activity peak of midges correlate with the sensitive wheat growth stage of heading (BBCH 50-59), the higher the crop damage. This is highlighted by the results of 2006 in the Tommi variant. In other cases, S. mosellana reached its activity peak much later (in 2005 during BBCH 65-69, resp. in 2006 on BBCH 73-75) and the ensuing crop damage was significantly smaller. In conclusion, the results of this survey in Central Germany stress the influence of changing agricultural conditions and regional cultivation concepts. They also recommend further studies that cover several years in open habitats

    PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor

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    PEX5 acts as a cycling receptor for import of PTS1 proteins into peroxisomes and as a co-receptor for PEX7, the PTS2 receptor, but the mechanism of cargo unloading has remained obscure. Using recombinant protein domains we show PEX5 binding to the PEX14N-terminal domain (PEX14N) has no effect on the affinity of PEX5 for a PTS1 containing peptide. PEX5 can form a complex containing both recombinant PTS1 cargo and endogenous PEX7-thiolase simultaneously but isolation of the complex via the PEX14 construct resulted in an absence of thiolase, suggesting a possible role for PEX14 in the unloading of PTS2 cargos

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    The expression of epidermal growth factor and transforming growth factor-α mRNA in the small intestine of suckling rats: organ culture study

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    AbstractEpidermal growth factor (EGF) and transforming growth factor-α (TGF-α) are associated with regulation of various gastrointestinal functions. In order to better understand their role in developing small intestine EGF, TGF-α and EGF-R steady-state mRNA levels and transcript stability were determined. Reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed that intestinal TGF-α mRNA levels were 10-fold higher in comparison with EGF mRNA. The primary intestinal culture technique was used to evaluate mRNA stability. The stability of TGF-α mRNA was remarkably lower than the stability of EGF mRNA. High levels of TGF-α mRNA accompanied by high degradation rate of this mRNA suggested a rapid turnover of intestinal TGF-α mRNA

    Independent and Opposing Roles For Btk and Lyn in B and Myeloid Signaling Pathways

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    Transphosphorylation by Src family kinases is required for the activation of Bruton's tyrosine kinase (Btk). Differences in the phenotypes of Btk−/− and lyn−/− mice suggest that these kinases may also have independent or opposing functions. B cell development and function were examined in Btk−/−lyn−/− mice to better understand the functional interaction of Btk and Lyn in vivo. The antigen-independent phase of B lymphopoiesis was normal in Btk−/−lyn−/− mice. However, Btk−/−lyn−/− animals had a more severe immunodeficiency than Btk−/− mice. B cell numbers and response to T cell–dependent antigens were reduced. Btk and Lyn therefore play independent or partially redundant roles in the maintenance and function of peripheral B cells. Autoimmunity, hypersensitivity to B cell receptor (BCR) cross-linking, and splenomegaly caused by myeloerythroid hyperplasia were alleviated by Btk deficiency in lyn−/− mice. A transgene expressing Btk at ∌25% of endogenous levels (Btklo) was crossed onto Btk−/− and Btk−/−lyn−/− backgrounds to demonstrate that Btk is limiting for BCR signaling in the presence but not in the absence of Lyn. These observations indicate that the net outcome of Lyn function in vivo is to inhibit Btk-dependent pathways in B and myeloid cells, and that Btklo mice are a useful sensitized system to identify regulatory components of Btk signaling pathways

    Depth of maximum of air-shower profiles at the Pierre Auger Observatory : II. Composition implications

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    Using the data taken at the Pierre Auger Observatory between December 2004 and December 2012, we have examined the implications of the distributions of depths of atmospheric shower maximum (Xmax), using a hybrid technique, for composition and hadronic interaction models. We do this by fitting the distributions with predictions from a variety of hadronic interaction models for variations in the composition of the primary cosmic rays and examining the quality of the fit. Regardless of what interaction model is assumed, we find that our data are not well described by a mix of protons and iron nuclei over most of the energy range. Acceptable fits can be obtained when intermediate masses are included, and when this is done consistent results for the proton and iron-nuclei contributions can be found using the available models. We observe a strong energy dependence of the resulting proton fractions, and find no support from any of the models for a significant contribution from iron nuclei. However, we also observe a significant disagreement between the models with respect to the relative contributions of the intermediate components.La lista completa de autores que integran el documento puede consultarse en el archivo.Facultad de Ciencias Exacta

    Depth of maximum of air-shower profiles at the Pierre Auger Observatory : II. Composition implications

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    Using the data taken at the Pierre Auger Observatory between December 2004 and December 2012, we have examined the implications of the distributions of depths of atmospheric shower maximum (Xmax), using a hybrid technique, for composition and hadronic interaction models. We do this by fitting the distributions with predictions from a variety of hadronic interaction models for variations in the composition of the primary cosmic rays and examining the quality of the fit. Regardless of what interaction model is assumed, we find that our data are not well described by a mix of protons and iron nuclei over most of the energy range. Acceptable fits can be obtained when intermediate masses are included, and when this is done consistent results for the proton and iron-nuclei contributions can be found using the available models. We observe a strong energy dependence of the resulting proton fractions, and find no support from any of the models for a significant contribution from iron nuclei. However, we also observe a significant disagreement between the models with respect to the relative contributions of the intermediate components.La lista completa de autores que integran el documento puede consultarse en el archivo.Facultad de Ciencias Exacta

    Spectral Calibration of the Fluorescence Telescopes of the Pierre Auger Observatory

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    We present a novel method to measure precisely the relative spectral response of the fluorescence telescopes of the Pierre Auger Observatory. We used a portable light source based on a xenon flasher and a monochromator to measure the relative spectral efficiencies of eight telescopes in steps of 5 nm from 280 nm to 440 nm. Each point in a scan had approximately 2 nm FWHM out of the monochromator. Different sets of telescopes in the observatory have different optical components, and the eight telescopes measured represent two each of the four combinations of components represented in the observatory. We made an end-to-end measurement of the response from different combinations of optical components, and the monochromator setup allowed for more precise and complete measurements than our previous multi-wavelength calibrations. We find an overall uncertainty in the calibration of the spectral response of most of the telescopes of 1.5% for all wavelengths; the six oldest telescopes have larger overall uncertainties of about 2.2%. We also report changes in physics measurables due to the change in calibration, which are generally small.Peer Reviewe
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