Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture

Milk fat depression in cows fed high-grain diets has been related to an increase in the concentration of trans-10 C-18:1 and trans-10, cis-12 conjugated linoleic acid (CLA) in milk. These fatty acids (FA) are produced as a result of the alteration in rumen biohydrogenation of dietary unsaturated FA. Because a reduction in ruminal pH is usually observed when high-concentrate diets are fed, the main cause that determines the alteration in the biohydrogenation pathways is not clear. The effect of pH (6.4 vs. 5.6) and dietary forage to concentrate ratios (F:C; 70:30 F:C vs. 30:70 F:C) on rumen microbial fermentation, effluent FA profile, and DNA concentration of bacteria involved in lipolysis and biohydrogenation processes were investigated in a continuous culture trial. The dual-flow continuous culture consisted of 2 periods of 8 d (5 d for adaptation and 3 d for sampling), with a 2 x 2 factorial arrangement of treatments. Samples from solid and liquid mixed effluents were taken for determination of total N, ammonia-N, and volatile fatty acid concentrations, and the remainder of the sample was lyophilized. Dry samples were analyzed for dry matter, ash, neutral and acid detergent fiber, FA, and purine contents. The pH 5.6 reduced organic matter and fiber digestibility, ammonia-N concentration and flow, and crude protein degradation, and increased nonammonia and dietary N flows. The pH 5.6 decreased the flow of C-18:0, trans-11 C-18:1 and cis-9, trans-11 CLA, and increased the flow of trans-10 C-18:1, C18:2n-6, C18:3n-3, trans-11, cis-15 C-18:2 and trans-10, cis-12 CLA in the 1 h after feeding effluent. The pH 5.6 reduced Anaerovibrio lipolytica (32.7 vs. 72.1 pg/10 ng of total DNA) and Butyrivibrio fibrisolvens vaccenic acid subgroup (588 vs. 1,394 pg/10 ng of total DNA) DNA concentrations. The high-concentrate diet increased organic matter and fiber digestibility, nonammonia and bacterial N flows, and reduced ammonia-N concentration and flow. The high-concentrate diet reduced trans-11 C-18:1 and trans-10 C-18:1, and increased C18:2n-6, C18:3n-3 and trans-10, cis-12 CLA proportions in the 1 h after feeding effluent. The increase observed in trans-10, cis-12 CLA proportion in the 1 h after feeding effluent due to the high-concentrate diet was smaller that that observed at pH 5.6. Results indicate that the pH is the main cause of the accumulation of trans-10 C-18:1 and trans-10, cis-12 CLA in the effluent, but the trans-10, cis-12 CLA proportion can be also affected by high levels of concentrate in the diet

Noise-induced phase transitions: Effects of the noises' statistics and spectrum

The local, uncorrelated multiplicative noises driving a second-order, purely noise-induced, ordering phase transition (NIPT) were assumed to be Gaussian and white in the model of [Phys. Rev. Lett. \textbf{73}, 3395 (1994)]. The potential scientific and technological interest of this phenomenon calls for a study of the effects of the noises' statistics and spectrum. This task is facilitated if these noises are dynamically generated by means of stochastic differential equations (SDE) driven by white noises. One such case is that of Ornstein--Uhlenbeck noises which are stationary, with Gaussian pdf and a variance reduced by the self-correlation time (\tau), and whose effect on the NIPT phase diagram has been studied some time ago. Another such case is when the stationary pdf is a (colored) Tsallis' (q)--\emph{Gaussian} which, being a \emph{fat-tail} distribution for (q>1) and a \emph{compact-support} one for (q<1), allows for a controlled exploration of the effects of the departure from Gaussian statistics. As done before with stochastic resonance and other phenomena, we now exploit this tool to study--within a simple mean-field approximation and with an emphasis on the \emph{order parameter} and the ``\emph{susceptibility}''--the combined effect on NIPT of the noises' statistics and spectrum. Even for relatively small (\tau), it is shown that whereas fat-tail noise distributions ((q>1)) counteract the effect of self-correlation, compact-support ones ((q<1)) enhance it. Also, an interesting effect on the susceptibility is seen in the last case.Comment: 6 pages, 10 figures, uses aipproc.cls, aip-8s.clo and aipxfm.sty. To appear in AIP Conference Proceedings. Invited talk at MEDYFINOL'06 (XV Conference on Nonequilibrium Statistical Mechanics and Nonlinear Physics

Stochastic Resonance: influence of a f−κf^{-\kappa} noise spectrum

Here, in order to study \textit{stochastic resonance} (SR) in a double-well potential when the noise source has a spectral density of the form $f^{-\kappa}$ with varying $\kappa$, we have extended a procedure, introduced by Kaulakys et al (Phys. Rev. E \textbf{70}, 020101 (2004)). In order to have an analytical understanding of the results, we have obtained an effective Markovian approximation, that allows us to make a systematic study of the effect of such kind of noises on the SR phenomenon. The comparison of numerical and analytical results shows an excellent qualitative agreement indicating that the effective Markovian approximation is able to correctly describe the general trends.Comment: 11 pages, 6 figures, submitted to Euro.Phys.J.

Increased intestinal carbonate precipitate abundance in the sea bream (Sparus aurata L.) in response to ocean acidification

Marine fish contribute to the carbon cycle by producing mineralized intestinal precipitates generated as by-products of their osmoregulation. Here we aimed at characterizing the control of epithelial bicarbonate secretion and intestinal precipitate presence in the gilthead sea bream in response to predicted near future increases of environmental CO2. Our results demonstrate that hypercapnia (950 and 1800 μatm CO2) elicits higher intestine epithelial HCO3- secretion ex vivo and a subsequent parallel increase of intestinal precipitate presence in vivo when compared to present values (440 μatm CO2). Intestinal gene expression analysis in response to environmental hypercapnia revealed the up-regulation of transporters involved in the intestinal bicarbonate secretion cascade such as the basolateral sodium bicarbonate co-transporter slc4a4, and the apical anion transporters slc26a3 and slc26a6 of sea bream. In addition, other genes involved in intestinal ion uptake linked to water absorption such as the apical nkcc2 and aquaporin 1b expression, indicating that hypercapnia influences different levels of intestinal physiology. Taken together the current results are consistent with an intestinal physiological response leading to higher bicarbonate secretion in the intestine of the sea bream paralleled by increased luminal carbonate precipitate abundance and the main related transporters in response to ocean acidification.Agência financiadora Fundacao para a Ciencia e a Tecnologia (FCT) SFRH/BD/113363/2015 PTDC/MAR-BIO/3034/2014 Fundacao para a Ciencia e a Tecnologia (FCT) UID/Multi/04326/2019 Ministry of Science and Higher Education, Polandinfo:eu-repo/semantics/publishedVersio

ApuA, a multifunctional x-glucan-degrading enzyme of Streptococcus suis, mediates adhesion to porcine epithelium and mucus

We have identified apuA in Streptococcus suis, which encodes a bifunctional amylopullulanase with conserved -amylase and pullulanase substrate-binding domains and catalytic motifs. ApuA exhibited properties typical of a Gram-positive surface protein, with a putative signal sequence and LPKTGE cell-wall-anchoring motif. A recombinant protein containing the predicted N-terminal -amylase domain of ApuA was shown to have -(1,4) glycosidic activity. Additionally, an apuA mutant of S. suis lacked the pullulanase -(1,6) glycosidic activity detected in a cell-surface protein extract of wild-type S. suis. ApuA was required for normal growth in complex medium containing pullulan as the major carbon source, suggesting that this enzyme plays a role in nutrient acquisition in vivo via the degradation of glycogen and food-derived starch in the nasopharyngeal and oral cavities. ApuA was shown to promote adhesion to porcine epithelium and mucus in vitro, highlighting a link between carbohydrate utilization and the ability of S. suis to colonize and infect the host

Spectroscopic Signatures of Electronic Excitations in Raman Scattering in Thin Films of Rhombohedral Graphite

Rhombohedral graphite features peculiar electronic properties, including persistence of low-energy surface bands of a topological nature. Here, we study the contribution of electron-hole excitations towards inelastic light scattering in thin films of rhombohedral graphite. We show that, in contrast to the featureless electron-hole contribution towards Raman spectrum of graphitic films with Bernal stacking, the inelastic light scattering accompanied by electron-hole excitations in crystals with rhombohedral stacking produces distinct features in the Raman signal which can be used both to identify the stacking and to determine the number of layers in the film.Comment: 15 pages in preprint format, 4 figures, accepted versio

Is UV laser ablation a suitable tool for geochemical analysis of organic rich source materials?

Abstract not available

Absolute reliability and concurrent validity of hand held dynamometry and isokinetic dynamometry in the hip, knee and ankle joint: Systematic review and meta-analysis

Indexación: Scopus.The purpose of the study is to establish absolute reliability and concurrent validity between hand-held dynamometers (HHDs) and isokinetic dynamometers (IDs) in lower extremity peak torque assessment. Medline, Embase, CINAHL databases were searched for studies related to psychometric properties in muscle dynamometry. Studies considering standard error of measurement SEM (%) or limit of agreement LOA (%) expressed as percentage of the mean, were considered to establish absolute reliability while studies using intra-class correlation coefficient (ICC) were considered to establish concurrent validity between dynamometers. In total, 17 studies were included in the meta-analysis. The COSMIN checklist classified them between fair and poor. Using HHDs, knee extension LOA (%) was 33.59%, 95% confidence interval (CI) 23.91 to 43.26 and ankle plantar flexion LOA (%) was 48.87%, CI 35.19 to 62.56. Using IDs, hip adduction and extension; knee flexion and extension; and ankle dorsiflexion showed LOA (%) under 15%. Lower hip, knee, and ankle LOA (%) were obtained using an ID compared to HHD. ICC between devices ranged between 0.62, CI (0.37 to 0.87) for ankle dorsiflexion to 0.94, IC (0.91to 0.98) for hip adduction. Very high correlation were found for hip adductors and hip flexors and moderate correlations for knee flexors/extensors and ankle plantar/dorsiflexors.https://www.degruyter.com/view/j/med.2017.12.issue-1/med-2017-0052/med-2017-0052.xm

Fuzzy Inference System for VOLT/VAR control in distribution substations in isolated power systems

This paper presents a fuzzy inference system for voltage/reactive power control in distribution substations. The purpose is go forward to automation distribution and its implementation in isolated power systems where control capabilities are limited and it is common using the same applications as in continental power systems. This means that lot of functionalities do not apply and computational burden generates high response times. A fuzzy controller, with logic guidelines embedded based upon heuristic rules resulting from operators at dispatch control center past experience, has been designed. Working as an on-line tool, it has been tested under real conditions and it has managed the operation during a whole day in a distribution substation. Within the limits of control capabilities of the system, the controller maintained successfully an acceptable voltage profile, power factor values over 0,98 and it has ostensibly improved the performance given by an optimal power flow based automation system

Meta-model Pruning

Large and complex meta-models such as those of Uml and its profiles are growing due to modelling and inter-operability needs of numerous\ud stakeholders. The complexity of such meta-models has led to coining\ud of the term meta-muddle. Individual users often exercise only a small\ud view of a meta-muddle for tasks ranging from model creation to construction\ud of model transformations. What is the effective meta-model that represents\ud this view? We present a flexible meta-model pruning algorithm and\ud tool to extract effective meta-models from a meta-muddle. We use\ud the notion of model typing for meta-models to verify that the algorithm\ud generates a super-type of the large meta-model representing the meta-muddle.\ud This implies that all programs written using the effective meta-model\ud will work for the meta-muddle hence preserving backward compatibility.\ud All instances of the effective meta-model are also instances of the\ud meta-muddle. We illustrate how pruning the original Uml metamodel\ud produces different effective meta-models