Cortical development associated with conjugation of Paramecium

The cortical cytoskeleton of Paramecium is characterized by a complex, polarized and asymmetrical organization. In order to analyse the cortical development of Paramecium tetraurelia during the sexual process of conjugation, different antibodies have been used to follow the development of five cortical components: basal bodies, ciliary rootlets, outer lattice, epiplasm and cytospindle. This study demonstrates that the cortex of Paramecium undergoes an elaborate development process following conjugation. Some of the cortical structures that are not renewed at any other moment of the cell cycle, are resorbed after conjugation and replaced by other newly formed ones. The reorganization of the cortical components occurs according to different morphogenetic waves that spread over the cell surface. The oral system and the preoral suture line act as morphogenetic epicentres.Junta de Andalucía y Departamento General de Investigación Científica y Técnica (DGICYT) PB91/062

La gestión del talento humano y la capacitación del personal en la Municipalidad provincial de Cajatambo, 2021

La investigación tuvo como objetivo principal, determinar la relación que existe entre la Gestión del Talento Humano y la Capacitación de Personal en la Municipalidad Provincial de Cajatambo, 2021. El estudio es de tipo aplicada, de diseño no experimental de corte transversal y de enfoque cuantitativo. La población está conformada por 32 trabajadores que laboran en la institución del área de administración, la técnica utilizada fue la encuesta cuyos cuestionarios estuvieron conformados por 20 ítems de la primera variable y 20 ítems para la segunda variable, para la confiabilidad de los instrumentos se utilizó el alfa de Cronbach con valores de 0.930 y 0.962 para cada variable respectivamente. En conclusión, mediante la prueba de Rho de Spearman se tuvo un coeficiente de correlación de 0.671 y una sigma bilateral de 0,000 por lo tanto, al ser menor al nivel de probabilidad de 0.05, se aceptó la hipótesis general, detallando que la Gestión del Talento Humano se relaciona significativamente con la Capacitación del Personal

Divergencias del alumnado y del profesorado universitario sobre las dificultades para aplicar la evaluación formativa

La evaluación formativa supone un claro cambio en la forma de plantear la evaluación en la docencia universitaria. Su aplicación no está exenta de cierta controversia, y sobre todo de dificultades, que hacen que alumnado y profesorado tengan distintas perspectivas. El objetivo del presente estudio ha sido comprobar las divergencias del alumnado y del profesorado cuando se diseña y se ponen en práctica sistemas de evaluación formativa. Se ha utilizado un cuestionario para el alumnado (N=3013) y los informes elaborados por el profesorado (N=46) aplicados durante el curso 2012-13. Los resultados muestran que la evaluación formativa es bastante exigente para el alumnado, aunque también muestran una alta satisfacción del alumnado con la misma, particularmente con la calificación. Un inconveniente que refleja el alumnado parece ser la acumulación de trabajo al final del proceso, lo cual resulta paradójico y requiere de una investigación más profunda en el futuro. El profesorado también muestra una alta satisfacción con este tipo de evaluación, aunque tengan una mayor carga de trabajo. Se observan escasas discrepancias entre los dos colectivos (alumnado y profesorado). En el estudio se proponen recomendaciones de puesta en práctica en cuanto al diseño y la planificación para investigaciones futuras. Las limitaciones se observan en cuanto a la población participante en el estudio, con el fin de que puedan generalizarse los resultados.Formative Assessment shows a clear change in how to approach the evaluation of university teaching. The application of Formative Assessment is not without some controversy, and above all difficulties due to students and teachers having different perspectives. The objective of the present study was to check the differences of students and teachers when formative assessment systems are designed and put into practice. A questionnaire was used for students (N = 3013) and a report prepared by the teachers (N = 46). Both were taken from the academic year 2012-13. The results show that formative assessment is quite demanding for students, but also that students are highly satisfied with it, particularly with grades. A problem that the students reflect seems to be the accumulation of work at the end of the process, which is paradoxical and will requires further research in the future. Teachers also show a high satisfaction with this type of assessment, even if they have a greater workload. There are few differences between the two groups (students and teachers). The study proposes recommendations for implementation in terms of design and planning for future research. The limitations of the research can be seen in terms of participating in the study population, so the results can be generalized

Association of Right Breast and Kidney Malignant Tumors. A Case Report. 2008

El cáncer renal de células granulares es un tumor que aparece con frecuencia en nuestra área, pero, pero encontrarlo asociado a células  ductales de mama es bastante  extraño. Se hace un reporte de caso de una paciente de 53 años quien fue diagnosticada con ambos tipos de tumores, confirmado histológicamente  en la parte derecha del cuerpo y con un período de ocho años entre ellos. Nada similar se encontrará en la literatura nacional e internacional consultada.Renal granular cells cancer is a frequently tumor encountered in our area, but to find it associated to breast ductal cells cancer is quite rare. We report a case of a 53 year old female patient, who was diagnosed with both kinds of tumors, histologically confirmed; on the rigth side of the body and with a period between them of 8 years. Nothing similar was found in the national and international consulted literature

Phenolic characterization of aging wine lees: correlation with antioxidant activities

Aging wine lees are water-wastes produced during the wine aging inside wood barrels that can be considered as alternative sources of bioactive compounds. Phenolic characterization and antioxidant activity (AA) measurements of wines lees solid-liquid extracts have been undertaken on a dry extract (DE) basis. Solvents with different polarities (water, methanol, ethanol, two hydroalcoholic mixtures and acetone) were used. Total phenolic (TPC) and total flavonoid contents (TFC) were determined. The mixture of 75:25(v/v) EtOH:H2O showed the highest values with 254mgGAE/gDE and 146mgCATE/gDE respectively. HORAC, HOSC and FRAP were used to determine the AA of the extracts being also highest for the mixture of 75:25(v/v) EtOH:H2O (4,690 µmolCAE/gDE, 4,527 µmolTE/gDE and 2,197 µmolTE/gDE, respectively). For ORAC method, methanol extract showed the best value with 2,771µmolTE /gDE. Correlations between TPC, TFC, phenolic compounds and AA were determined. Most relevant compounds contributing to AA were identified using data from mass spectrometry, being mainly anthocyanins.2019-04-0

Microwave and ultrasound pre-treatments to enhance anthocynins extraction from different wine lees.

Wine lees are rich in anthocyanins (AC), natural colorants with health promoting properties. The extraction kinetics of AC from different wine lees in conventional solid-liquid extraction were studied for the first time. The influence of parameters such as temperature, solid-liquid ratio (RS-L) and type of solvent (hydro-alcoholic mixtures) was also studied. Furthermore, microwaves (MW) and ultrasounds (US) were used as pre-treatments (a prior step to the conventional extraction) in order to increase AC yield. Maximum extraction yield (2.78 mgMALVIDIN-EQUIVALENTS/gDRY-LEES) was achieved after 15 minutes at 25ºC, with a RS-L of 1/10 (g/mL) and with a 50%vol. ethanol mixture. When MW were used AC extraction yield was doubled (6.20 mgMALVIDIN-EQUIVALENTS/gDRY-LEES) and the required time to achieve a constant yield was reduced (from 15 min to 90s). Meanwhile, US only shortened extraction time in less proportion (from 15 to 5 min). Putative identification of main extract compounds was performed by LC/MS-MS.2019-09-012019-09-0

Instrumentos de evaluación : uso y competencia del profesorado universitario en su aplicación

En los últimos años se está produciendo en la Educación Superior un cambio de modelo contrapuesto al tradicional, orientado fundamentalmente hacia la acumulación de conocimientos por parte del alumno, lo cual demanda al profesorado el uso de nuevas metodologías y sistemas de evaluación. La finalidad de este trabajo fue conocer los instrumentos de evaluación que utiliza el profesorado de actividad física y el nivel de competencia que considera tener cuando los implementa. Se diseñó un cuestionario que fue cumplimentado por 345 profesores universitarios. Los resultados indicaron que el profesorado aplica una amplia gama de herramientas de evaluación que permiten potenciar diferentes competencias de los alumnos. También se encontraron correlaciones positivas entre la frecuencia de uso y competencia del profesorado y entre el uso de instrumentos coherentes con procesos formativos. Finalmente, destaca el uso de instrumentos más competenciales en estudios de magisterio y la formación psico-pedagógica del profesora o marca diferencias positivasIn recent years, a change of educational model been taking place in higher education, one opposed to traditional models and oriented fundamentally toward knowledge accumulation by students. This requires teaching staff to use new methodologies and evaluation systems. The purpose of this study is to understand the evaluation instruments used by P.E. teachers and the level of aptitude that they consider themselves to have when implementing them. A questionnaire was designed and completed by 345 university professors. The results suggest that teachers apply a wide range of assessment tools that allow them to enhance different student skills. We also found positive correlations between the frequency of use and competence of the professor and between the use of coherent instruments and training processes. Finally, this work highlights the use of increasingly competency-based instruments in teaching studies and the psycho-pedagogical training of teachers, which indicates positive difference

Contribution of Phenotypic Heterogeneity to plant colonization by Pseudomonas syringae

In bacterial clonal populations, cell to cell differences can be originated by the response to different stimuli present in the environment. However, the sources of variation may not always be directly correlated with stimuli. In some cases, these differences are merely a consequence of the noise in gene expression or in others, a programmed event under genetic or epigenetic control. The presence of different phenotypes can allow some individuals to survive sudden environmental changes (risk-spreading) and can also lead to the division of labour between individuals. The relevance of this process has been demonstrated in Salmonella and other human pathogens for the expression of virulence genes and has been linked to the establishment of a successful infection. However, little is known about the importance of this process in the colonization of the plant tissue. In the phytopathogenic bacteria Pseudomonas syringae we have demonstrated that the T3SS show phenotypic heterogeneity during the colonization of the plant. We have also established that flagella is expressed and displays phenotypic heterogeneity during colonization of the apoplast. These processes are counter-regulated. Nonetheless, all possible combinations for T3SS and flagella expression are formed within the apoplastic population, including T3SSON/FlagellaON and T3SSOFF/FlagellaOFF bacteria. We show that expression and function of these virulence-relevant loci impact bacterial fitness and describe how plant defences modulate their expression at the population level. All these observations support the notion that the phenotypic heterogeneity is a relevant process for the adaptation of P. syringae to the plant host.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Polyphenol-Rich Extracts Obtained from Winemaking Waste Streams as Natural Ingredients with Cosmeceutical Potential

Producción CientíficaPhenolics present in grapes have been explored as cosmeceutical principles, due to their antioxidant activity and ability to inhibit enzymes relevant for skin ageing. The winemaking process generates large amounts of waste, and the recovery of bioactive compounds from residues and their further incorporation in cosmetics represents a promising market opportunity for wine producers and may contribute to a sustainable development of the sector. The extracts obtained from grape marc and wine lees, using solid–liquid (SL) extraction with and without microwave (MW) pretreatment of the raw material, were characterized in terms of antioxidant activity through chemical (ORAC/HOSC/HORAC) and cell-based (keratinocytes—HaCaT; fibroblasts—HFF) assays. Furthermore, their inhibitory capacity towards specific enzymes involved in skin ageing (elastase; MMP-1; tyrosinase) was evaluated. The total phenolic and anthocyanin contents were determined by colorimetric assays, and HPLC–DAD–MS/MS was performed to identify the main compounds. The MW pretreatment prior to conventional SL extraction led to overall better outcomes. The red wine lees extracts presented the highest phenolic content (3 to 6-fold higher than grape marc extracts) and exhibited the highest antioxidant capacity, being also the most effective inhibitors of elastase, MMP-1 and tyrosinase. The results support that winemaking waste streams are valuable sources of natural ingredients with the potential for cosmeceutical applications.European project WineSense (project FP7-386 MC-IAPP)Junta de Castilla y León (project VA040U16)Ministerio de Educación y Formación Profesional (fellowship FPU13/04678)Portuguese Fundação para a Ciência e Tecnologia (FCT) (grants PEst-OE/EQB/LA0004/2011 and UID/Multi/04462/2013)iNOVA4Health (grant UID/Multi/04462/2013

Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain

BACKGROUND Despite great effort and investment incurred over decades to control bovine tuberculosis (bTB), it is still one of the most important zoonotic diseases in many areas of the world. Test-and-slaughter strategies, the basis of most bTB eradication programs carried out worldwide, have demonstrated its usefulness in the control of the disease. However, in certain countries, eradication has not been achieved due in part to limitations of currently available diagnostic tests. In this study, results of in-vivo and post-mortem diagnostic tests performed on 3,614 animals from 152 bTB-infected cattle herds (beef, dairy, and bullfighting) detected in 2007-2010 in the region of Castilla y León, Spain, were analyzed to identify factors associated with positive bacteriological results in cattle that were non-reactors to the single intradermal tuberculin test, to the interferon-gamma (IFN-γ) assay, or to both tests applied in parallel (Test negative/Culture + animals, T-/C+). The association of individual factors (age, productive type, and number of herd-tests performed since the disclosure of the outbreak) with the bacteriology outcome (positive/negative) was analyzed using a mixed multivariate logistic regression model. RESULTS The proportion of non-reactors with a positive post-mortem result ranged from 24.3% in the case of the SIT test to 12.9% (IFN-γ with 0.05 threshold) and 11.9% (95% CI 9.9-11.4%) using both tests in parallel. Older (>4.5 years) and bullfighting cattle were associated with increased odds of confirmed bTB infection by bacteriology, whereas dairy cattle showed a significantly lower risk. Ancillary use of IFN-γ assay reduced the proportion of T-/C + animals in high risk groups. CONCLUSIONS These results demonstrate the likelihood of positive bacteriological results in non-reactor cattle is influenced by individual epidemiological factors of tested animals. Increased surveillance on non-reactors with an increased probability of being false negative could be helpful to avoid bTB persistence, particularly in chronically infected herds. These findings may aid in the development of effective strategies for eradication of bTB in Spain