Terrestrial arachnids

74 p. : ill. ; 26 cm.Includes bibliographical references (p. 72-74)."Three new genera and seven new species of the arachnid order Trigonotarbida are described based on remarkably well preserved fossils from the late Middle Devonian (Givetian) of Gilboa, New York: Gilboarachne griersoni, Gelasinotarbus reticulatus, G. bonamoae, G. bifidus, G. heptops, G.? fimbriunguis, and Aculeatarbus depressus. A brief review of other known Devonian trigonotarbids is presented, and certain misconceptions about the order are rectified, including the nature of the eyes, chelicerae, claws, and abdominal segmentation. Trigonotarbida is shown by cladistic analysis to be the plesiomorphic sister-group of Araneae + Amblypygi + Uropygi + Schizomida"--P. 2

Core transcriptional regulatory circuitry in human hepatocytes

We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator–gene interactions. Autoregulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes

Core transcriptional regulatory circuitry in human hepatocytes

We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator–gene interactions. Autoregulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes

Temperature and Pressure Dependence of the Optical Absorption and Luminescence of a Defect in TlCl

The luminescence associated with an absorption band at about 3.0 eV in chalcogen-doped TlCl has been observed. The temperature and pressure coefficients of the positions of the absorption and emission peaks have been found to have "anomalous" negative signs. The temperature coefficient of the absorption peak is dominated by the effect of thermal expansion, the band shifting toward the blue as the lattice expands. The emission peak exhibits very little temperature dependence. Some arguments are presented in favor of interpreting this band as an exciton band associated with an anion vacancy (α band) instead of an F band. Lattice relaxation may cause the emission to occur at Cl2− molecule-ions near the vacancy.This article is from Physical Review 159 (1967): 687, doi:10.1103/PhysRev.159.687. Posted with permission.</p

DETECTION OF SOLAR-LIKE OSCILLATIONS, OBSERVATIONAL CONSTRAINTS, AND STELLAR MODELS FOR θ CYG, THE BRIGHTEST STAR OBSERVED BY THE KEPLER MISSION

θ Cygni is an F3 spectral type magnitude V = 4.48 main-sequence star that was the brightest star observed by the original Kepler spacecraft mission. Short-cadence (58.8 s) photometric data using a custom aperture were first obtained during Quarter 6 (2010 June–September) and subsequently in Quarters 8 and 12–17. We present analyses of solar-like oscillations based on Q6 and Q8 data, identifying angular degree l = 0, 1, and 2 modes with frequencies of 1000–2700 μHz, a large frequency separation of 83.9 ± 0.4 μHz, and maximum oscillation amplitude at frequency νmax = 1829 ± 54 μHz. We also present analyses of new ground-based spectroscopic observations, which, combined with interferometric angular diameter measurements, give Teff = 6697 ± 78 K, radius 1.49 ± 0.03 Re, [Fe/H] = −0.02 ± 0.06 dex, and log g = 4.23 ± 0.03. We calculate stellar models matching these constraints using the Yale Rotating Evolution Code and the Asteroseismic Modeling Portal. The best-fit models have masses of 1.35–1.39 Me and ages of 1.0–1.6 Gyr. θ Cyg’s Teff and log g place it cooler than the red edge of the γ Doradus instability region established from pre-Kepler ground-based observations, but just at the red edge derived from pulsation modeling. The pulsation models show γ Dor gravity modes driven by the 1 convective blocking mechanism, with frequencies of 1–3 cycles per day (11 to 33 μHz). However, gravity modes were not seen in Kepler data; one signal at 1.776 cycles per day (20.56 μHz) may be attributable to a faint, possibly background, binary

Control of Transcription by Cell Size

Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. Here we show that ploidy-associated changes in gene expression reflect transcriptional adjustment to a larger cell size, implicating cellular geometry as a key parameter in gene regulation. Using RNA-seq, we identified genes whose expression was altered in a tetraploid as compared with the isogenic haploid. A significant fraction of these genes encode cell surface proteins, suggesting an effect of the enlarged cell size on the differential regulation of these genes. To test this hypothesis, we examined expression of these genes in haploid mutants that also produce enlarged size. Surprisingly, many genes differentially regulated in the tetraploid are identically regulated in the enlarged haploids, and the magnitude of change in gene expression correlates with the degree of size enlargement. These results indicate a causal relationship between cell size and transcription, with a size-sensing mechanism that alters transcription in response to size. The genes responding to cell size are enriched for those regulated by two mitogen-activated protein kinase pathways, and components in those pathways were found to mediate size-dependent gene regulation. Transcriptional adjustment to enlarged cell size could underlie other cellular changes associated with polyploidy. The causal relationship between cell size and transcription suggests that cell size homeostasis serves a regulatory role in transcriptome maintenance.National Institutes of Health (U.S.) (grant GM035010)National Institutes of Health (U.S.) (grant GM040266

Prostanoid receptor EP1 and Cox-2 in injured human nerves and a rat model of nerve injury: a time-course study

BACKGROUND: Recent studies show that inflammatory processes may contribute to neuropathic pain. Cyclooxygenase-2 (Cox-2) is an inducible enzyme responsible for production of prostanoids, which may sensitise sensory neurones via the EP1 receptor. We have recently reported that while macrophages infiltrate injured nerves within days of injury, they express increased Cox-2-immunoreactivity (Cox-2-IR) from 2 to 3 weeks after injury. We have now investigated the time course of EP1 and Cox-2 changes in injured human nerves and dorsal root ganglia (DRG), and the chronic constriction nerve injury (CCI) model in the rat. METHODS: Tissue sections were immunostained with specific antibodies to EP1, Cox-2, CD68 (human macrophage marker) or OX42 (rat microglial marker), and neurofilaments (NF), prior to image analysis, from the following: human brachial plexus nerves (21 to 196 days post-injury), painful neuromas (9 days to 12 years post-injury), avulsion injured DRG, control nerves and DRG, and rat CCI model tissues. EP1 and NF-immunoreactive nerve fibres were quantified by image analysis. RESULTS: EP1:NF ratio was significantly increased in human brachial plexus nerve fibres, both proximal and distal to injury, in comparison with uninjured nerves. Sensory neurones in injured human DRG showed a significant acute increase of EP1-IR intensity. While there was a rapid increase in EP1-fibres and CD-68 positive macrophages, Cox-2 increase was apparent later, but was persistent in human painful neuromas for years. A similar time-course of changes was found in the rat CCI model with the above markers, both in the injured nerves and ipsilateral dorsal spinal cord. CONCLUSION: Different stages of infiltration and activation of macrophages may be observed in the peripheral and central nervous system following peripheral nerve injury. EP1 receptor level increase in sensory neurones, and macrophage infiltration, appears to precede increased Cox-2 expression by macrophages. However, other methods for detecting Cox-2 levels and activity are required. EP1 antagonists may show therapeutic effects in acute and chronic neuropathic pain, in addition to inflammatory pain

Achieving Education for Sustainable Development (ESD) in Early Childhood Education Through Critical Reflection in Transformative Learning

The central role of education in creating a more sustainable future has been already recognized by educators and policy-makers alike. This chapter argues that this can only be truly achieved through the efforts of teachers in implementing an “education of a different kind,” a general educational shift that seeks to encompass a converging transformation of the priorities and mindsets of education professionals. In this regard, the professional preparation of teachers, as the leading actors in shaping children’s learning processes, and their continuous professional development are vital considerations for Education for Sustainable Development (ESD) to be successfully achieved. Linking transformative learning and ESD has emerged as a distinct and useful pedagogy because they both support the process of critically examining habits of mind, then revising these habits and acting upon the revised point of view. This study aims to describe and evaluate the potential of transformative learning in innovating mainstream education toward sustainability by focusing on the role of critical reflection in a capacity building research project realized in Turkey. The data was gathered from 24 early childhood educators using a mixed-method research design involving learning diaries, a learning activities survey, and follow-up interviews. This chapter identified content, context, and application method of the in-service training as factors that have contributed to the reflective practices of the participants. In addition, presenting the implications regarding the individual differences in how learners engage in critical reflection practices, this research offers a framework for a content- and process-based approach derived from Mezirow’s conception of critical reflection