Contribution of agronomy to land management issues - A Comparison of five interdisciplinary PhD theses

An introductory literature review highlights the growing attention within the processes taking place at farming region and landscape scale beside the classical spatial scales at cultivated/experimental plot level. This recent evolution in agronomy finds its origin in newly emerging land management issues. Meanwhile, geography and other disciplines are stressing the need for a greater integration of multifunctional agricultural activities into the decision-making processes at the various levels of land management, such as provinces, municipalities or watersheds. This requires also that studies on farmland management include explicitly the different environmental and social contexts influencing farming activities. In this paper we aim to analyse how recent agronomic oriented research are facing and supporting various land management issues. We have compared five interdisciplinary PhD theses examining their definitions and methods of analysis for: the farming system, the local land management issues at stake, the spatial scale selected for the study, the stakeholders' involvement and the interaction with other disciplines. Common issues which emerged from this comparison are delivery of agro-environmental services, sustainable land management and landscape conservation. Multiple spatial levels were considered, which included at least one administrative unit of policy decision/implementation. Consequently, the explicit (re)definition of some agronomic concepts and methods was needed. Regarding the interdisciplinary framework, the theses have stressed the interactions among agronomy, geography and ecology. All theses aimed at delivering tools for decision-making support, mainly in the form of cartography. Nevertheless the participation of local stakeholders was generally included as a final step; herewith the settings of stakeholders' involvement were various. In conclusion, we discuss how the produced knowledge has enhanced the land management issues in local planning tools. On these bases, we stress finally the issues at stake to strengthen the roles and contributions of agronomic oriented education and research to agricultural land management and development. (Résumé d'auteur

Relationship between Exposure to Vector Bites and Antibody Responses to Mosquito Salivary Gland Extracts

Mosquito-borne diseases are major health problems worldwide. Serological responses to mosquito saliva proteins may be useful in estimating individual exposure to bites from mosquitoes transmitting these diseases. However, the relationships between the levels of these IgG responses and mosquito density as well as IgG response specificity at the genus and/or species level need to be clarified prior to develop new immunological markers to assess human/vector contact. To this end, a kinetic study of antibody levels against several mosquito salivary gland extracts from southeastern French individuals living in three areas with distinct ecological environments and, by implication, distinct Aedes caspius mosquito densities were compared using ELISA. A positive association was observed between the average levels of IgG responses against Ae. caspius salivary gland extracts and spatial Ae. caspius densities. Additionally, the average level of IgG responses increased significantly during the peak exposure to Ae. caspius at each site and returned to baseline four months later, suggesting short-lived IgG responses. The species-specificity of IgG antibody responses was determined by testing antibody responses to salivary gland extracts from Cx. pipiens, a mosquito that is present at these three sites at different density levels, and from two other Aedes species not present in the study area (Ae. aegypti and Ae. albopictus). The IgG responses observed against these mosquito salivary gland extracts contrasted with those observed against Ae. caspius salivary gland extracts, supporting the existence of species-specific serological responses. By considering different populations and densities of mosquitoes linked to environmental factors, this study shows, for the first time, that specific IgG antibody responses against Ae. caspius salivary gland extracts may be related to the seasonal and geographical variations in Ae. caspius density. Characterisation of such immunological-markers may allow the evaluation of the effectiveness of vector-control strategies or estimation of the risk of vector-borne disease transmission

Impact of Sonic Hedgehog-dependent sphenoid bone defect on craniofacial growth

International audienceObjectivesThe main objective of this study was to evaluate how an apparently minor anomaly of the sphenoid bone, observed in a haploinsufficient mouse model for Sonic Hedgehog (Shh), affects the growth of the adult craniofacial region. This study aims to provide valuable information to orthodontists when making decisions regarding individuals carrying SHH mutation.Materials and MethodsThe skulls of embryonic, juvenile and adult mice of two genotypes (Shh heterozygous and wild type) were examined and measured using landmark‐based linear dimensions. Additionally, we analysed the clinical characteristics of a group of patients and their relatives with SHH gene mutations.ResultsIn the viable Shh+/− mouse model, bred on a C57BL/6J background, we noted the presence of a persistent foramen at the midline of the basisphenoid bone. This particular anomaly was attributed to the existence of an ectopic pituitary gland. We discovered that this anomaly led to premature closure of the intrasphenoidal synchondrosis and contributed to craniofacial deformities in adult mice, including a longitudinally shortened skull base. This developmental anomaly is reminiscent of that commonly observed in human holoprosencephaly, a disorder resulting from a deficiency in SHH activity. However, sphenoid morphogenesis is not currently monitored in individuals carrying SHH mutations.ConclusionHaploinsufficiency of Shh leads to isolated craniofacial skeletal hypoplasia in adult mouse. This finding highlights the importance of radiographic monitoring of the skull base in all individuals with SHH gene mutations

TRF2 inhibition triggers apoptosis and reduces tumourigenicity of human melanoma cells

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RNA Viral Communities Are Structured by Host Plant Phylogeny in Oak and Conifer Leaves

Wild plants can suffer devastating diseases, experience asymptomatic persistent infections, and serve as reservoirs for viruses of agricultural crops; however, we have a limited understanding of the natural plant virosphere. To access representatives of locally and globally distinct wild plants and investigate their viral diversity, we extracted and sequenced double-stranded RNA from leaves from 16 healthy oak and conifer trees in the University of California–Davis Arboretum (Davis, CA, U.S.A.). From de novo assemblies, we recovered 389 RNA-dependent RNA polymerase gene sequences from 384 putative viral species, and identified 580 putative viral contigs via virus prediction software followed by manual confirmation of virus annotation. Based on similarity to known viruses, most recovered viruses were predicted to infect plants or fungi, with the highest diversity and abundance observed in the Totiviridae and Mitoviridae families. Phyllosphere viral community composition differed significantly by host plant phylogeny, suggesting the potential for host-specific viromes. The phyllosphere viral community of one oak tree differed substantially from other oak viral communities and contained a greater proportion of putative mycoviral sequences, potentially due to the tree's more advanced senescence at the time of sampling. These results suggest that oak and conifer trees harbor a vast diversity of viruses with as-yet-unknown roles in plant health and phyllosphere microbial ecology. [Graphic: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license

Disrupted hypothalamo-pituitary axis in association with reduced SHH underlies the pathogenesis of NOTCH-deficiency

International audienceContext: In human, Sonic Hedgehog, SHH, haploinsufficiency is the predominant cause of holoprosencephaly, a structural malformation of the forebrain midline characterised by phenotypic heterogeneity and incomplete penetrance. The NOTCH signalling pathway has recently been associated with holoprosencephaly, in humans, but the precise mechanism involving NOTCH signalling during early brain development remains unknown.Objective: The aim of this study was to evaluate the relationship between SHH and NOTCH signalling in order to determine the mechanism by which NOTCH dysfunction could cause midline malformations of the forebrain.Design: In this study, we have used a chemical inhibition approach in the chick model and a genetic approach in the mouse model. We reported results obtained from clinical diagnosis of a cohort composed of 141 holoprosencephaly patients.Results: We demonstrated that inhibition of NOTCH signalling in chick embryos as well as in mouse embryos induces a specific downregulation of SHH in the anterior hypothalamus. Our data in the mouse also revealed that the pituitary gland was the most sensitive tissue to Shh insufficiency and that haploinsufficiency of the SHH and NOTCH signalling pathways synergized to produce a malformed pituitary gland. Analysis of a large holoprosencephaly cohort revealed that some patients possessed multiple heterozygous mutations in several regulators of both pathways.Conclusions: These results provided new insights into molecular mechanisms underlying the extreme phenotypic variability observed in human holoprosencephaly. They showed how haploinsufficiency of the SHH and NOTCH activity could contribute to specific congenital hypopituitarism that was associated with a sella turcica defect

The mangroves in Guadeloupe: a suspended ecosystem under anthropic pressures

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Targeted High-throughput Sequencing for Hematological Malignancies: A GBMHM Survey of Practice and Cost Evaluation in France

The objective of this study was to assess the clinical impact and financial costs of next-generation sequencing (NGS) in 5 categories of pediatric and adult hematological cancers. NGS prescriptions were prospectively collected from 26 laboratories, with varied technical and reporting practice (all or only significant targets). Impact was defined by the identification of (1) an actionable mutation, (2) a mutation with prognostic and/or theranostic value, and/or (3) a mutation allowing nosological refinement, reported by local investigators. A microcosting study was undertaken in 4 laboratories, identifying the types and volumes of resources required for each procedural step. Individual index prescriptions for 3961 patients were available for impact analysis on the management of myeloid disorders (two thirds) and, mainly mature B, lymphoid disorders (one third). NGS results were considered to impact the management for 73.4% of prescriptions: useful for evaluation of prognostic risk in 34.9% and necessary for treatment adaptation (actionable) in 19.6%, but having no immediate individual therapeutic impact in 18.9%. The average overall cost per sample was 191 € for the restricted mature lymphoid amplicon panel. Capture panel costs varied from 369 € to 513 €. Unit costs varied from 0.5 € to 5.7 € per kb sequenced, from 3.6 € to 11.3 € per target gene/hot-spot sequenced and from 4.3 € to 73.8 € per target gene/hot-spot reported. Comparable costs for the Amplicon panels were 5–8 € per kb and 10.5–14.7 € per target gene/hot-spot sequenced and reported, demonstrating comparable costs with greater informativity/flexibility for capture strategies. Sustainable funding of precision medicine requires a transparent discussion of its impact on care pathways and its financial aspects