STUDI EKSPLANATORI FAKTOR-FAKTOR YANG MEMPENGARUHI MINAT BELI VASELINE MEN FACE WASH DI SURABAYA

Abstrak - Penelitian ini bertujuan untuk mengetahui pengaruh dari Consumer Values pada Attitude Toward Behavior serta pengaruh Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, dan Past Experience sehingga mempengaruhi Purchase Intention Vaseline MEN Face Wash di Surabaya. Penelitian ini merupakan replikasi penelitian oleh Kim, H.Y., Chung, Jae-Eun (2011) dengan judul “Consumer purchase intention for organic personal care products”, sekaligus modifikasi dari penelitian oleh Ting Chi,. Peter K. (2011) dengan judul “Understanding consumer perceived value of casual sportswear: An empirical study”. Pada penelitian ini terdapat 10 variabel dengan 31 indikator. Jumlah sampel yang diambil sebesar 205 responden dengan kriteria spesifik yaitu berjenis kelamin pria, berusia 18-40 tahun, pernah menggunakan produk Vaseline MEN face wash, melakukan pembelian produk Vaseline MEN face wash dengan dana pribadi, pernah menggunakan produk perawatan pribadi selama 3 bulan terakhir. Metode pengolahan data menggunakan Structural Equation Modeling dengan software Lisrel 8.80. Hasil yang didapat dalam penelitian ini serupa dengan penelitian Kim et al., (2011). Temuan dari penelitian menunjukkan bahwa 4 elemen consumer values yaitu price, social, emotional, quality berpengaruh positif terhadap Attitude Toward Behavior. Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, dan Past Experiences berpengaruh positif terhadap Purchase Intention produk Vaseline MEN Face Wash di Surabaya. Kata Kunci: Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, Past Experiences, Purchase Intention, Structural Equation Model ABSTRACT - This study was aimed at identifying the influence of Consumer Values to Attitude Toward Behavior and Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, and Past Experience . The study was a replication of the previous one conducted by Kim, H.Y., Chung, Jae-Eun (2011) entitled “Consumer purchase intention for organic personal care products” also modification of the previous one conducted by Ting Chi,. Peter K. (2011) entitled “Understanding consumer perceived value of casual sportswear: An empirical study”. There were 10 variables with 31 indicators. The samples were taken from 205 respondents who had specific criteria, i.e., Men, 10-40 years old, and having used Vaseline MEN face wash, bougth with his own money, used personal care products the last three months. The data was analyzed using Structural Equation Modeling by means of Lisrel 8.80. The results of this study similar from those of Kim et al., (2011). This study found out that there was 4 elements of Consumer Value, i.e., price, social, emotional, quality significant influence among Attitude Toward Behavior. Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, and Past Experiences significant influence among Purchase Intention of Vaseline MEN face wash in Surabaya. Key words: Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, Past Experiences, Purchase Intention, Structural Equation Mode

STUDI EKSPLANATORI FAKTOR-FAKTOR YANG MEMPENGARUHI MINAT BELI VASELINE MEN FACE WASH DI SURABAYA

Abstrak - Penelitian ini bertujuan untuk mengetahui pengaruh dari Consumer Values pada Attitude Toward Behavior serta pengaruh Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, dan Past Experience sehingga mempengaruhi Purchase Intention Vaseline MEN Face Wash di Surabaya. Penelitian ini merupakan replikasi penelitian oleh Kim, H.Y., Chung, Jae-Eun (2011) dengan judul “Consumer purchase intention for organic personal care products”, sekaligus modifikasi dari penelitian oleh Ting Chi,. Peter K. (2011) dengan judul “Understanding consumer perceived value of casual sportswear: An empirical study”. Pada penelitian ini terdapat 10 variabel dengan 31 indikator. Jumlah sampel yang diambil sebesar 205 responden dengan kriteria spesifik yaitu berjenis kelamin pria, berusia 18-40 tahun, pernah menggunakan produk Vaseline MEN face wash, melakukan pembelian produk Vaseline MEN face wash dengan dana pribadi, pernah menggunakan produk perawatan pribadi selama 3 bulan terakhir. Metode pengolahan data menggunakan Structural Equation Modeling dengan software Lisrel 8.80. Hasil yang didapat dalam penelitian ini serupa dengan penelitian Kim et al., (2011). Temuan dari penelitian menunjukkan bahwa 4 elemen consumer values yaitu price, social, emotional, quality berpengaruh positif terhadap Attitude Toward Behavior. Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, dan Past Experiences berpengaruh positif terhadap Purchase Intention produk Vaseline MEN Face Wash di Surabaya. Kata Kunci: Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, Past Experiences, Purchase Intention, Structural Equation Model ABSTRACT - This study was aimed at identifying the influence of Consumer Values to Attitude Toward Behavior and Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, and Past Experience . The study was a replication of the previous one conducted by Kim, H.Y., Chung, Jae-Eun (2011) entitled “Consumer purchase intention for organic personal care products” also modification of the previous one conducted by Ting Chi,. Peter K. (2011) entitled “Understanding consumer perceived value of casual sportswear: An empirical study”. There were 10 variables with 31 indicators. The samples were taken from 205 respondents who had specific criteria, i.e., Men, 10-40 years old, and having used Vaseline MEN face wash, bougth with his own money, used personal care products the last three months. The data was analyzed using Structural Equation Modeling by means of Lisrel 8.80. The results of this study similar from those of Kim et al., (2011). This study found out that there was 4 elements of Consumer Value, i.e., price, social, emotional, quality significant influence among Attitude Toward Behavior. Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, and Past Experiences significant influence among Purchase Intention of Vaseline MEN face wash in Surabaya. Key words: Attitude Toward Behavior, Perceived Behavioral Control, Subjective Norms, Past Experiences, Purchase Intention, Structural Equation Mode

A novel regulatory circuit specifies cell fate in the Arabidopsis root epidermis

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75165/1/j.1399-3054.2006.00606.x.pd

Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis

The CAPRICE (CPC)-like MYB gene family encodes R3-type MYB transcription factors in Arabidopsis. There are six additional CPC-like MYB sequences in the Arabidopsis genome, including TRYPTICHON (TRY), ENHANCER OF TRY AND CPC1 and 2 (ETC1 and ETC2), ENHANCER OF TRY AND CPC3/CPC-LIKE MYB3 (ETC3/CPL3), and TRICHOMELESS1 and 2 (TCL1 and TCL2). We independently identified CPC-LIKE MYB4 (CPL4), which was found to be identical to TCL2. RT-PCR analysis showed that CPL4 is strongly expressed in shoots, including true leaves, but not in roots. Promoter-GUS analyses indicated that CPL4 is specifically expressed in leaf blades. Although CPC expression was repressed in 35S::ETC1, 35S::ETC2 and 35S::CPL3 backgrounds, CPL4 expression was not affected by ETC1, ETC2 or CPL3 over-expression. Notably, several chimeric transcripts may result from inter-genic alternative splicing of CPL4 and ETC2, two tandemly repeated genes on chromosome II. At least two chimeric transcripts named CPL4-α and CPL4-β are expected to encode complete CPC-like MYB proteins

Distinct relationships between GLABRA2 and single-repeat R3 MYB transcription factors in the regulation of trichome and root hair patterning in Arabidopsis

• The patterning of epidermal cell types in Arabidopsis is an excellent model for studying the molecular basis of cell specification. Trichome and root hair formation is controlled by a transcriptional activator complex that induces the homeobox gene GLABRA2 (GL2) and some single-repeat R3 MYB genes (single MYB ). However, it remains unclear how the actions of GL2 and single MYBs are coordinated to regulate epidermal patterning. • GL2 is thought to act downstream of single MYBs to regulate trichome and root hair development. In order to test this hypothesis genetically, double and higher order mutants between gl2 and single myb were generated. • In these mutants, the glabrous phenotypes observed in the gl2 single mutants were partially recovered, suggesting that single MYBs may not act solely through GL2 to regulate trichome development. On the other hand, double and higher order mutants between gl2 and single myb phenocopied the root hair phenotype of gl2 single mutants, suggesting that GL2 and single MYBs act in a common pathway to regulate root hair patterning. • These findings reveal distinct relationships between GL2 and single MYBs in the regulation of trichome vs root hair development, and provide new insights into the molecular mechanism of epidermal patterning.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/78641/1/j.1469-8137.2009.03067.x.pd

Transcriptome Analysis of Arabidopsis Wild-Type and gl3–sst sim Trichomes Identifies Four Additional Genes Required for Trichome Development

Transcriptome analyses have been performed on mature trichomes isolated from wild-type Arabidopsis leaves and on leaf trichomes isolated from the gl3–sst sim double mutant, which exhibit many attributes of immature trichomes. The mature trichome profile contained many highly expressed genes involved in cell wall synthesis, protein turnover, and abiotic stress response. The most highly expressed genes in the gl3–sst sim profile encoded ribosomal proteins and other proteins involved in translation. Comparative analyses showed that all but one of the genes encoding transcription factors previously found to be important for trichome formation, and many other trichome-important genes, were preferentially expressed in gl3–sst sim trichomes. The analysis of genes preferentially expressed in gl3–sst sim led to the identification of four additional genes required for normal trichome development. One of these was the HDG2 gene, which is a member of the HD–ZIP IV transcription factor gene family. Mutations in this gene did not alter trichome expansion, but did alter mature trichome cell walls. Mutations in BLT resulted in a loss of trichome branch formation. The relationship between blt and the phenotypically identical mutant, sti, was explored. Mutations in PEL3, which was previously shown to be required for development of the leaf cuticle, resulted in the occasional tangling of expanding trichomes. Mutations in another gene encoding a protein with an unknown function altered trichome branch formation

MUM ENHANCERS are important for seed coat mucilage production and mucilage secretory cell differentiation in Arabidopsis thaliana

Pollination triggers not only embryo development but also the differentiation of the ovule integuments to form a specialized seed coat. The mucilage secretory cells of the Arabidopsis thaliana seed coat undergo a complex differentiation process in which cell growth is followed by the synthesis and secretion of pectinaceous mucilage. A number of genes have been identified affecting mucilage secretory cell differentiation, including MUCILAGE-MODIFIED4 (MUM4). mum4 mutants produce a reduced amount of mucilage and cloning of MUM4 revealed that it encodes a UDP-L-rhamnose synthase that is developmentally up-regulated to provide rhamnose for mucilage pectin synthesis. To identify additional genes acting in mucilage synthesis and secretion, a screen for enhancers of the mum4 phenotype was performed. Eight mum enhancers (men) have been identified, two of which result from defects in known mucilage secretory cell genes (MUM2 and MYB61). Our results show that, in a mum4 background, mutations in MEN1, MEN4, and MEN5 lead to further reductions in mucilage compared to mum4 single mutants, suggesting that they are involved in mucilage synthesis or secretion. Conversely, mutations in MEN2 and MEN6 appear to affect mucilage release rather than quantity. With the exception of men4, whose single mutant exhibits reduced mucilage, none of these genes have a single mutant phenotype, suggesting that they would not have been identified outside the compromised mum4 background

A Gene Regulatory Network for Root Epidermis Cell Differentiation in Arabidopsis

The root epidermis of Arabidopsis provides an exceptional model for studying the molecular basis of cell fate and differentiation. To obtain a systems-level view of root epidermal cell differentiation, we used a genome-wide transcriptome approach to define and organize a large set of genes into a transcriptional regulatory network. Using cell fate mutants that produce only one of the two epidermal cell types, together with fluorescence-activated cell-sorting to preferentially analyze the root epidermis transcriptome, we identified 1,582 genes differentially expressed in the root-hair or non-hair cell types, including a set of 208 “core” root epidermal genes. The organization of the core genes into a network was accomplished by using 17 distinct root epidermis mutants and 2 hormone treatments to perturb the system and assess the effects on each gene's transcript accumulation. In addition, temporal gene expression information from a developmental time series dataset and predicted gene associations derived from a Bayesian modeling approach were used to aid the positioning of genes within the network. Further, a detailed functional analysis of likely bHLH regulatory genes within the network, including MYC1, bHLH54, bHLH66, and bHLH82, showed that three distinct subfamilies of bHLH proteins participate in root epidermis development in a stage-specific manner. The integration of genetic, genomic, and computational analyses provides a new view of the composition, architecture, and logic of the root epidermal transcriptional network, and it demonstrates the utility of a comprehensive systems approach for dissecting a complex regulatory network