The analysis of physicochemical characteristics of pig farm seepage and its possible impact on the receiving natural environment

Pig farm seepage poses an environmental risk, considering that seepage can be generally applied on land without appropriate agronomic criteria or may accidentally spill on the natural environment. These environmental risks include increasing oxygen demand, nutrient loading of water-bodies, promoting toxic and algal blooms eutrophication, thus, leading to a destabilized environment. This research was conducted to determine the impact that the pig farm seepage may have the receiving environment based on the analyses of the physicochemical parameters of the adjacent environments. Wastewater and soil samples were collected between the periods of March 2013 to August 2013 and wastewater was analyzed for pH, temperature, Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Dissolved Solids (TDS), salinity, turbidity, Dissolved Oxygen (DO), NO3, NO2, and PO4 3−. The results for wastewater samples for BOD (163 mg/L to 3350 mg/L), TDS (0.77 g/L to 6.48 mg/L), COD (210 mg/L to 9400 mg/L), and NO3 (55 mg/L to 1680 mg/L), were higher than the maximum permissible limits. Results of soil samples for TDS (0.01g/L to 0.88 g/L), COD (40 mg/L to 304 mg/L), NO3 (32.5 mg/L to 475 mg/L), and NO2 (7.35 mg/L to 255 mg/L) were also higher than recommended limits. The results revealed that the seepage from pig farm degraded the natural environment by causing eutrophication, promote toxic and algal blooms, increase oxygen demand and thus destabilize the homeostatic balance of the receiving environment.Keywords: Physicochemical parameters, pollution, soil, wastewater, seepage, pig farm, environmen

Country-Specific Interactions Among Private Investment Economic Growth And Poverty Level In Three Selected Sub-Saharan African Countries. (1985-2010).

This study specifically examines the relations among private investment, economic growth and poverty level in Nigeria and its two neighbouring sub-Saharan African (SSA) countries of Benin Republic and Cameroon between the periods 1985 and 2010. The study employed Vector Error Correction Model using data extracted from the World Development Indicators. The study revealed that the relationships among private investment, economic growth, and poverty level did not follow expected pattern in the three countries. The results in Benin Republic show that increase in private investment and reduction in poverty level rather than increase real GDP growth, reduced real GDP growth overtime while the results obtained for Cameroon and Nigeria show that increase in private investment increased poverty level and reduction in poverty level reduced private participation in business in Cameroon and Nigeria. The study suggests a weak relation between private investment and economic growth or poverty level in the three economies.The study therefore recommends measures such as macroeconomic stability and adequate legal system that will ensure proper take off of private investment to boost economic growth and reduce poverty level in these three economies. Keywords: Private investment, public investment, economic growth, poverty and Vector Error Correction

Community composition and functions of endophytic bacteria of Bt maize

We investigated the potential effects of genetic modification of Bt maize on the community composition and functions of bacterial endophytes associated with transgenic maize (Bt MON 810) in comparison with its isogenic parental line at two developmental stages. Bacterial isolates were obtained from transgenic (Bt) and non-transgenic (non-Bt) maize at 50- and 90-day-old developmental stages. Isolated bacterial endophytes were screened for their capabilities in phosphate solubilisation, nitrogen fixation, production of antifungal metabolites and production of indole acetic acid. After molecular identification, 60 isolates were obtained and clustered into 19 and 18 operational taxonomic units from 50- and 90-day-old maize, respectively. The isolates belonged to the genera Bacillus, Pantoea, Serratia, Yersinia, Enterobacter, Pseudomonas, Acinetobacter and Stenotrophomonas. Functional attributes and diversity of the isolated endophytes at both developmental stages were not significantly different for both maize varieties. However, functional attributes were significantly affected by plant growth stage. Isolates from younger plants were more efficient producers of indole acetic acid, but exhibited little or no capabilities for nitrogen fixation, phosphate solubilisation and antifungal activity in both maize genotypes. Based on these outcomes, Bt modification in maize does not seem to affect the community composition or functional attributes of bacterial endophytes. Significance: • Bt modification in maize does not affect the ecological guild or functional attributes of cultivable bacterial endophytes

Assessing the impact of rice cultivation and off-season period on dynamics of soil enzyme activities and bacterial communities in two agro-ecological regions of Mozambique

Soil ecosystem perturbation due to agronomic practices can negatively impact soil productivity by altering the diversity and function of soil health determinants. Currently, the influence of rice cultivation and off-season periods on the dynamics of soil health determinants is unclear. Therefore, soil enzyme activities (EAs) and bacterial community compositions in rice-cultivated fields at postharvest (PH) and after a 5-month off-season period (5mR), and fallow-fields (5-years-fallow, 5YF; 10-years-fallow, 10YF and/or one-year-fallow, 1YF) were assessed in two agroecological regions of Mozambique. EAs were mostly higher in fallow fields than in PH, with significant (p < 0.05) differences detected for -glucosidase and acid phosphatase activities. Only -glucosidase activity was significantly (p < 0.05) different between PH and 5mR, suggesting that -glucosidase is responsive in the short-term. Bacterial diversity was highest in rice-cultivated soil and correlated with NO3 , NH4 + and electrical conductivity. Differentially abundant genera, such as Agromyces, Bacillus, Desulfuromonas, Gaiella, Lysobacter, Micromonospora, Norcadiodes, Rubrobacter, Solirubrobacter and Sphingomonas were mostly associated with fallow and 5mR fields, suggesting either negative effects of rice cultivation or the fallow period aided their recovery. Overall, rice cultivation and chemical parameters influenced certain EAs and shaped bacterial communities. Furthermore, the 5-month off-season period facilitates nutrient recovery and proliferation of plant-growth-promoting bacteriainfo:eu-repo/semantics/publishedVersio

Assessing the mycotoxicological risk from consumption of complementary foods by infants and young children in Nigeria

This study assessed, for the first time, the mycotoxicological risks from consumption of complementary foods by infants and young children in Nigeria. Molds belonging to Aspergillus aculeatinus, A. flavus, A. luchuensis, A. tubingensis, A. welwitschiae and Geotrichum candidum were recovered from the complementary foods. Twenty-eight major mycotoxins and derivatives, and another 109 microbial metabolites including chloramphenicol (a bacterial metabolite), were quantified in 137 food samples by LC-MS/MS. Aflatoxins and fumonisins co-contaminated 42% of the cereal- and nut-based food samples, at mean concentrations exceeding the EU limits of 0.1 and 200 μg/kg set for processed baby foods by 300 and six times, respectively. Milk contained mainly beauvericin, chloramphenicol and zearalenone. The trichothecenes, T-2 and HT-2 toxins, were quantified only in infant formula and at levels three times above the EU indicative level of 15 μg/kg for baby food. Chronic exposure estimate to carcinogenic aflatoxin was high causing low margin of exposure (MOE). Exposures to other mycotoxins either exceeded the established reference values by several fold or revealed low MOEs, pointing to important health risks in this highly vulnerable population. The observed mycotoxin mixtures may further increase risks of adverse health outcomes of exposure; this warrants urgent advocacy and regulatory interventions.</p

Analysis of squat shear wall with different dimensions and position of opening under different type of static loads

Shear walls are usually used in high-rise building or building on high frequency of wind area as the structural element to restrain lateral forces. Openings are created on the shear wall for the architecture, ventilation or mechanical and electrical purposes. With the existence of the opening, the strength of the wall is reduced by the reduction in concrete area and the discontinuity of the reinforcement due to opening which may lead to structural failure. The main objective is to study the effect of size and position of the opening towards the structural behavior of the shear wall under different type of static loads. The analysis is done using the software ANSYS12.0. The samples are SW1, SW2, SW3, SW4, SW5, SW6, SW7 and SW8 where SW1 is solid shear wall while SW2, SW3, SW4, SW5, SW6, SW7 and SW8 are shear walls with different size and position of opening. They are analyzed by using 2 different types of loads which are uniformly distributed axial load and uniformly lateral load. From the same magnitude of loads applied towards the shear walls, they are compared by the cracking pattern and the stress distribution. Under both axial and lateral loads, it shows a significance results that the shear wall with greater opening size shows less efficiency. The position of opening further from the support shows a more significance effect towards the strength of the wall from axial load but opposite from the lateral load. Besides, the closer the position of the shear wall to the load, the less efficient it is. As a conclusion from the results, the most suitable position of the opening on the shear wall is further from the support and the loads and it shows that there is a significance effect even from a smallest opening

High-Throughput Sequence Analyses of Bacterial Communities and Multi-Mycotoxin Profiling During Processing of Different Formulations of Kunu, a Traditional Fermented Beverage

Kunu is a traditional fermented single or mixed cereals-based beverage popularly consumed in many parts of West Africa. Presently, the bacterial community and mycotoxin contamination profiles during processing of various kunu formulations have never been comprehensively studied. This study, therefore, investigated the bacterial community and multi-mycotoxin dynamics during the processing of three kunu formulations using high-throughput sequence analysis of partial 16S rRNA gene (hypervariable V3-V4 region) and liquid chromatography tandem mass spectrometry (LC-MS/MS), respectively. A total of 2,303 operational taxonomic units (OTUs) were obtained across six processing stages in all three kunu formulations. Principal coordinate analysis biplots of the Bray-Curtis dissimilarity between bacterial communities revealed the combined influences of formulations and processing steps. Taxonomically, OTUs spanned 13 phyla and 486 genera. Firmicutes (phylum) dominated (relative abundance) most of the processing stages, while Proteobacteria dominated the rest of the stages. Lactobacillus (genus taxa level) dominated most processing stages and the final product (kunu) of two formulations, whereas Clostridium sensu stricto (cluster 1) dominated kunu of one formulation, constituting a novel observation. We further identified Acetobacter, Propionibacterium, Gluconacetobacter, and Gluconobacter previously not associated with kunu processing. Shared phylotypes between all communities were dominated by lactic acid bacteria including species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, and Weissella. Other shared phylotypes included notable acetic acid bacteria and potential human enteric pathogens. Ten mycotoxins [3-Nitropropionic acid, aflatoxicol, aflatoxin B1 (AFB1), AFB2, AFM1, alternariol (AOH), alternariolmethylether (AME), beauvericin (BEAU), citrinin, and moniliformin] were quantified at varying concentrations in ingredients for kunu processing. Except for AOH, AME, and BEAU that were retained at minimal levels of &lt; 2 μg/kg in the final product, most mycotoxins in the ingredients were not detectable after processing. In particular, mycotoxin levels were substantially reduced by fermentation, although simple dilution and sieving also contributed to mycotoxin reduction. This study reinforces the perception of kunu as a rich source of bacteria with beneficial attributes to consumer health, and provides in-depth understanding of the microbiology of kunu processing, as well as information on mycotoxin contamination and reduction during this process. These findings may aid the development of starter culture technology for safe and quality kunu production

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Image_5_Soil nutrient management influences diversity, community association and functional structure of rhizosphere bacteriome under vegetable crop production.JPEG

IntroductionRhizosphere bacterial communities play a crucial role in promoting plant and soil ecosystem health and productivity. They also have great potential as key indicators of soil health in agroecosystems. Various environmental factors affect soil parameters, which have been demonstrated to influence soil microbial growth and activities. Thus, this study investigated how rhizosphere bacterial community structure and functions are affected by agronomic practices such as organic and conventional fertiliser application and plant species types.MethodsRhizosphere soil of vegetable crops cultivated under organic and conventional fertilisers in different farms was analysed using high-throughput sequencing of the 16S rRNA gene and co-occurrence network pattern among bacterial species. The functional structure was analysed with PICRUSt2 pipeline.ResultsOverall, rhizosphere bacterial communities varied in response to fertiliser type, with soil physicochemical parameters, including NH4, PO4, pH and moisture content largely driving the variations across the farms. Organic farms had a higher diversity richness and more unique amplicon sequence variants than conventional farms. Bacterial community structure in multivariate space was highly differentiated across the farms and between organic and conventional farms. Co-occurrence network patterns showed community segmentation for both farms, with keystone taxa more prevalent in organic than conventional farms.DiscussionModule hub composition and identity varied, signifying differences in keystone taxa across the farms and positive correlations between changes in microbial composition and ecosystem functions. The organic farms comprised functionally versatile communities characterised by plant growth-promoting keystone genera, such as Agromyces, Bacillus and Nocardioides. The results revealed that organic fertilisers support high functional diversity and stronger interactions within the rhizosphere bacterial community. This study provided useful information about the overall changes in soil microbial dynamics and how the changes influence ecosystem functioning under different soil nutrient management and agronomic practices.</p

Image_2_Soil nutrient management influences diversity, community association and functional structure of rhizosphere bacteriome under vegetable crop production.JPEG

IntroductionRhizosphere bacterial communities play a crucial role in promoting plant and soil ecosystem health and productivity. They also have great potential as key indicators of soil health in agroecosystems. Various environmental factors affect soil parameters, which have been demonstrated to influence soil microbial growth and activities. Thus, this study investigated how rhizosphere bacterial community structure and functions are affected by agronomic practices such as organic and conventional fertiliser application and plant species types.MethodsRhizosphere soil of vegetable crops cultivated under organic and conventional fertilisers in different farms was analysed using high-throughput sequencing of the 16S rRNA gene and co-occurrence network pattern among bacterial species. The functional structure was analysed with PICRUSt2 pipeline.ResultsOverall, rhizosphere bacterial communities varied in response to fertiliser type, with soil physicochemical parameters, including NH4, PO4, pH and moisture content largely driving the variations across the farms. Organic farms had a higher diversity richness and more unique amplicon sequence variants than conventional farms. Bacterial community structure in multivariate space was highly differentiated across the farms and between organic and conventional farms. Co-occurrence network patterns showed community segmentation for both farms, with keystone taxa more prevalent in organic than conventional farms.DiscussionModule hub composition and identity varied, signifying differences in keystone taxa across the farms and positive correlations between changes in microbial composition and ecosystem functions. The organic farms comprised functionally versatile communities characterised by plant growth-promoting keystone genera, such as Agromyces, Bacillus and Nocardioides. The results revealed that organic fertilisers support high functional diversity and stronger interactions within the rhizosphere bacterial community. This study provided useful information about the overall changes in soil microbial dynamics and how the changes influence ecosystem functioning under different soil nutrient management and agronomic practices.</p