Exploration of antimicrobial resistant communities in Montpellier area. Evaluation of innovative microbial indicators of anthropic pressure - Poster

National audienc

Dynamique de l’antibiorésistance dans un petit fleuve urbain en lien avec les conditions hydrologiques et hydrochimiques

International audienc

Environmental Antimicrobial Resistance in a Small Urban Mediterranean River: A Focus on Endemic Beta-Lactamases in Clinically Relevant Bacteria

International audienceAquatic ecosystems subjected to anthropic pressures are likely hotspots for emergence or dissemination of antimicrobial resistant bacteria. The city of Montpellier is located on a Mediterranean climate watershed that undergoes strong demographic pressures. The aim of the study is to explore antimicrobial resistance, particularly those of clinical concern, in urban rivers flowing in this urban area. The method developed herein to explore antimicrobial resistance is based on cultural and molecular approaches completed by hydrological, hydrogeological, climatic, and physico-chemical data. Hospital vicinity and urbanization density significantly increase cultivable bacterial community, fecal bacteria from human origin, and prevalence of β-lactamases and extended-spectrum β-lactamases encoding-genes without an increase in 16S rDNA gene abundance. A total of 22 multidrug Enterobacterales have been isolated. All Escherichia coli (n = 10) and Klebsiella pneumoniae (n = 6) isolated on a made-house media carried β-lactamases genes, blaCTX-M being the most prevalent (87%), followed by blaTEM (56%) and blaSHV (37%), 56% of these strains carrying two or three of these genes. In urban settings, water quality and infectious risk are generally linked to wastewater treatment plants effluents. This study shows that running waters in urbanized area contribute to the dissemination of antimicrobial resistance, making these environments a reservoir for resistant bacteria with important consideration

High-resolution EEG.

High-resolution EEG recording has become standard in many experimental studies on human brain function and has found its place in the routine presurgical workup of patients with focal epilepsy in several clinical centers. The main aim of high-resolution EEG is source localization with methods that have become increasingly robust and precise. However, high-resolution EEG also allows a spatial analysis of EEG and evoked potentials on the scalp level, thereby identifying topographic features of the scalp potential field. Their value in understanding the dynamics of large-scale networks of the human brain and as markers for neuropsychiatric diseases has been increasingly demonstrated. This chapter discusses the advantages and limitations of such spatial analysis methods and the information that can be gained from them. It also shows that the spatial frequency of the scalp potential field is higher than previously assumed and discusses the consequences regarding the number of channels required to properly capture these spatial frequencies

Congenital bile duct cyst (BDC) is a more indolent disease in children compared to adults, except for Todani type IV-A BDC: Results of the European multicenter study of the French Surgical Association

Aim To compare clinical presentation, operative management and short- and long-term outcomes of congenital bile duct cysts (BDC) in adults with children. Methods Retrospective multi-institutional Association Francaise de Chirurgie study of Todani types I+IVB and IVA BDC. Results During the 37-year period to 2011, 33 centers included 314 patients (98 children; 216 adults). The adult population included more high-risk patients, with more active, more frequent prior treatment (47.7% vs 11.2%; p < 0.0001), more complicated presentation (50.5% vs 35.7%; p = 0.015), more synchronous biliary cancer (11.6% vs 0%; p = 0.0118) and more major surgery (23.6% vs 2%; p < 0.0001), but this latter feature was only true for type I+IVB BDC. Compared to children, the postoperative morbidity (48.1% vs 20.4%; p < 0.0001), the need for repeat procedures and the status at follow-up were worse in adults (27% vs 8.8%; p = 0.0009). However, severe postoperative morbidity and fair or poor status at follow-up were not statistically different for type IVA BDC, irrespective of patients' age. Synchronous cancer, prior HBP surgery and Todani type IVA BDC were independent predictive factors of poor or fair long-term outcome. Conclusion BDC is a more indolent disease in children compared to adults, except for Todani type IV-A BDC