Chickpea rhizobia symbiosis genes are highly conserved across multiple Mesorhizobium species

ABSTRACT Chickpea has been considered as a restrictive host for nodulation by rhizobia. However, recent studies have reported that several Mesorhizobium species may effectively nodulate chickpea. With the purpose of investigating the evolutionary relationships between these different species with the ability of nodulating the same host, we analysed 21 Portuguese chickpea rhizobial isolates. Symbiosis genes nifH and nodC were sequenced and used for phylogenetic studies. Symbiotic effectiveness was determined to evaluate its relationship with symbiosis genes. The comparison of 16S rRNA gene-based phylogeny with the phylogenies based on symbiosis genes revealed evidence of lateral transfer of symbiosis genes across different species. Chickpea is confirmed as a nonpromiscuous host. Although chickpea is nodulated by many different species, they share common symbiosis genes, suggesting recognition of only a few Nod factors by chickpea. Our results suggest that sequencing of nifH or nodC genes can be used for rapid detection of chickpea mesorhizobia

Museology 4.0. Real-world example

[EN] This document will address the practical application in real use cases of Virtual Reality, Augmented Reality and other avant-garde interactive and immersive technologies, both in the context of Museum 4.0 and in spaces of heritage interest such as sites, monumental cities or xacobeas routes. It will be explained, with real and recent examples, how Xoia Extending Reality addresses the new technological needs of the Museum 4.0, with the aim of improving the interpretation, study, dissemination and promotion of our History and Heritage through the use of the new ICT, especially Augmented Reality and Virtual Reality, focusing on the user experience and the quality of what is disclosed. For this, success stories developed by Xoia are described, such as the exhibition coordinated by the “Xunta de Galicia”, "Galicia, un relato no mundo", the augmented reality tourist experience "Viaxando polo Pontedeume medieval", developed for the city council of Pontedeume in a context of promotion of the Saint James Way, among other projects to enhance heritage, culture and tourism, developed with city councils, universities and / or other institutions, innovatively linking tourism, technology and culture in order to disseminate in an innovative way and enhancing learning, reaching an increasingly digital audience. Finally, practical examples of technological solutions applied to the context of COVID -19 will be cited.[ES] En la presente comunicación se va a abordar la aplicación práctica en casos de uso reales de la Realidad Virtual, Aumentada y otras tecnologías interactivas e inmersivas de vanguardia, tanto en el contexto de Museo 4.0 como en espacios de interés patrimonial como pueden ser yacimientos, ciudades monumentales o rutas xacobeas. Se explicará, con ejemplos reales y recientes como Xoia Extending Reality afronta las nuevas necesidades tecnológicas del Museo 4.0, con el objetivo de mejorar la interpretación, estudio, divulgación y promoción de nuestra Historia y Patrimonio mediante el empleo de las NTIC, especialmente Realidad Aumentada y Virtual, centrándose en la experiencia de usuario y en la calidad de lo divulgado. Para ello se describen casos de éxito desarrollados por Xoia en el ámbito museístico como la exposición coordinada por la Xunta de Galicia, “Galicia un relato no mundo” o la experiencia turística de Realidad Aumentada “Viaxando polo Pontedeume medieval”, desarrollada para el ayuntamiento de Pontedeume en un contexto de promoción del camino de Santiago, entre otros proyectos de puesta en valor patrimonial, cultural y turística, desarrollados con ayuntamientos, universidades y/o otras instituciones, vinculando de forma innovadora el turismo, tecnología y cultura con la finalidad de divulgar de forma novedosa y potenciando el aprendizaje, logrando llegar a un público cada vez más digital. Por último se citará ejemplos prácticos de soluciones tecnológicas aplicadas al contexto del Covid -19.González Rivas, A.; Vazquez Dios, X. (2022). Museología 4.0. Un ejemplo Real. En CIMED21 - I Congreso internacional de museos y estrategias digitales. Editorial Universitat Politècnica de València. 201-218. https://doi.org/10.4995/CIMED21.2021.1265620121

Economia brasileira: transição para uma Economia Verde?

Este artigo visa considerar duas questões. Primeiro, discute-se se o que a Economia Verde tenta propor é um conteúdo “mais do mesmo” acerca das discussões ambientais desde a década de 70 do último século, em que as proposições sobre o desenvolvimento sustentável foram sua maior expressão, mas agora com um novo apelo: a crise ambiental é inevitável e, ao invés de ela ser necessariamente um problema econômico, pode vir a ser uma oportunidade de mercado. Nessa perspectiva, a segunda questão é analisada a partir de dados e informações relacionados ao comportamento do setor produtivo brasileiro, indagando-se se a economia brasileira segue o caminho de uma Economia Verde. Os dados e perspectivas dos investimentos que eram planejados até 2015 não permitem afirmar que a economia brasileira caminha na direção de uma Economia Verde, ou se segue a marcha de uma trajetória para isso. Palavras-chave: Economia Verde; economia brasileira; oportunidades econômicasTÍTULO EM INGLÊSBrazilian economy: transition to a Green Economy?AbstractThis article aims at considering two questions. First, we discuss whether the Green Economy is not attempting to propose a content that is "more of the same" about the environmental discussions that have been taking place since the 1970s, of which the propositions of sustainable development were the greatest expression, but now with a new appeal: the environmental crisis is inevitable and instead of necessarily being an economic problem, could end up being a market opportunity. In this perspective, the second question is examined based on data and information concerning the behavior of the Brazilian productive sector, in which we question if the Brazilian economy follows the path of a Green Economy. The data and prospects of the investments that were planned until  2015 do not allow us to claim that the Brazilian economy is moving towards a Green Economy or that it is following the trend of a transition to it. Keywords: Green Economy; Brazilian economy; economic opportunitiesClassificação JEL: O44, Q56Artigo recebido em dez. 2013 e aceito para publicação em set. 2015

Effects of temperature and photoperiod on daily activity rhythms of Lutzomyia longipalpis (Diptera: Psychodidae)

Made available in DSpace on 2015-05-04T17:07:27Z (GMT). No. of bitstreams: 2 license.txt: 1914 bytes, checksum: 7d48279ffeed55da8dfe2f8e81f3b81f (MD5) gustavo_rivasetal_IOC_2014.pdf: 728382 bytes, checksum: f0dd4d5e2dd3f6987745ff64d4383f08 (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM)/CNPq. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM)/CNPq. Rio de Janeiro, RJ, Brasil.Background: Insect vectors have been established as models in Chronobiology for many decades, and recent studies have demonstrated a close relationship between the circadian clock machinery, daily rhythms of activity and vectorial capacity. Lutzomyia longipalpis, the primary vector of Leishmania (Leishmania) infantum in the New World, is reported to have crepuscular/nocturnal activity in the wild. However, most of these studies applied hourly CDC trap captures, which is a good indicative of L. longipalpis behaviour, but has limited accuracy due to the inability to record the daily activity of a single insect during consecutive days. In addition, very little is known about the activity pattern of L. longipalpis under seasonal variations of average temperature and day length in controlled laboratory conditions. Methods: We recorded the locomotor activity of L. longipalpis males under different artificial regimes of temperature and photoperiod. First, in order to test the effects of temperature on the activity, sandflies were submitted to regimes of light/dark cycles similar to the equinox photoperiod (LD 12:12) combined with different constant temperatures (20°C, 25°C and 30°C). In addition, we recorded sandfly locomotor activity under a mild constant temperature (25°C with different day length regimes: 8 hours, 12 hours and 16 hours). Results: L. longipalpis exhibited more activity at night, initiating dusk-related activity (onset time) at higher rather than lower temperatures. In parallel, changes of photoperiod affected anticipation as well as all the patterns of activity (onset, peak and offset time). However, under LD 16:08, sandflies presented the earliest values of maximum peak and offset times, contrary to other regimes. Conclusions: Herein, we showed that light and temperature modulate L. longipalpis behaviour under controlled laboratory conditions, suggesting that sandflies might use environmental information to sustain their crepuscular/ nocturnal activity, as well as other important aspects as mating and host-seeking at appropriate times in different seasons. Our results depict previously unappreciated aspects of the L. longipalpis daily rhythms of activity that might have important epidemiological implications

Despesas com gestão ambiental nos estados da Amazônia Legal

Os governos, mediante legislações específicas, preveem e destinam recursos financeiros para a gestão ambiental. Considerando isto e a necessidade de verificar como esse recurso é alocado, o presente artigo teve por objetivo analisar o comportamento das despesas com gestão ambiental no orçamento dos estados da Amazônia Legal entre os anos de 2005 a 2015, identificando como cada estado designou e aplicou recursos para a gestão ambiental dentre as suas subfunções. Utilizou-se pesquisa documental-descritiva, de cunho quantitativo; e dados de despesas totais e de despesas com gestão ambiental, na forma de despesa liquidada, extraídas do site do Tesouro Nacional. Os estados do Mato Grosso, Pará, Amazonas e Acre são os que mais destinaram recursos para a área ambiental. Quanto à participação da despesa com gestão ambiental nos orçamentos governamentais, observou-se que a gestão ambiental correspondeu a menos de 1% dos orçamentos dos estados da Amazônia Legal no período analisad

Levels of Cognitive Congruence between Managers and Team Members' Perceptions of Cooperation at Work

Much of the research on the psychological dynamics of performance teams suffers from the following limitations: consideration of only one theoretical framework and analysis of just one perspective (e.g., manager-coach or team member). To address these shortcomings, this study used a Global Cooperation concept that synthesized five psychological frameworks: coordination, cohesion, cooperation, integration, and identification. The objective of this study was to examine the level of congruence-symmetry between the two perspectives and the tendency for managers-coaches and team members to reduce cognitive dissonance in the perception of global cooperation. To this end, 108 managers-coaches and members of performance teams were studied (range: 23−60 years old) using a Cooperative Workteam Questionnaire (CWQ). Results revealed that the greatest amount of asymmetry was observed in Global Cooperation and Emotional Cooperation, while less asymmetry was found in Personal Growth, and good congruence-fit in Conditioned Cooperation. Results are discussed in terms of their theoretical meaning and practical implications for interventions on performance teams

Adrenomedullin : a marker of impaired hemodynamics, organ dysfunction, and poor prognosis in cardiogenic shock

Background: The clinical CardShock risk score, including baseline lactate levels, was recently shown to facilitate risk stratification in patients with cardiogenic shock (CS). As based on baseline parameters, however, it may not reflect the change in mortality risk in response to initial therapies. Adrenomedullin is a prognostic biomarker in several cardiovascular diseases and was recently shown to associate with hemodynamic instability in patients with septic shock. The aim of our study was to evaluate the prognostic value and association with hemodynamic parameters of bioactive adrenomedullin (bio-ADM) in patients with CS. Methods: CardShock was a prospective, observational, European multinational cohort study of CS. In this sub-analysis, serial plasma bio-ADM and arterial blood lactate measurements were collected from 178 patients during the first 10 days after detection of CS. Results: Both bio-ADM and lactate were higher in 90-day non-survivors compared to survivors at all time points (P <0.05 for all). Lactate showed good prognostic value during the initial 24 h (AUC 0.78 at admission and 0.76 at 24 h). Subsequently, lactate returned normal ( 55.7 pg/mL) at 48 h compared to those with low bio-ADM levels (49.1 vs. 22.6%, P = 0.001). High levels of bio-ADM were associated with impaired cardiac index, mean arterial pressure, central venous pressure, and systolic pulmonary artery pressure during the study period. Furthermore, high levels of bio-ADM at 48 to 96 h were related to persistently impaired cardiac and end-organ function. Conclusions: Bio-ADM is a valuable prognosticator and marker of impaired hemodynamics in CS patients. High levels of bio-ADM may show shock refractoriness and developing end-organ dysfunction and thus help to guide therapeutic approach in patients with CS.Peer reviewe