Characterizing early cancer biology through multiplexed and multiscale super-resolution microscopy technique development

This thesis work highlights technique development and emerging applications for improving super-resolution microscopy (SRM) throughput, analysis and applications in studying early cancer biology

Automation in Leather Making

Content: In most of the tanneries, water and chemicals are added manually in the tanning drum and pH of the float / leather is adjusted. Addition of correct amount of process recipe are necessary for better processing of the hides and minimizing wastage of utility, thereby controlling pollution load in effluent. However, fugitive-emission from process and drains accumulate ammonia, hydrogen-sulphide and volatile organic compounds which contribute bad odor in tannery as well as in wastewater-treatment premises causing problems to occupational health & safety of workers. Both local and supervisory control stations are employed to monitor and accurately manage the unit operations. The objective of this work is to produce consistent quality of leathers and to provide a healthy environment through automatic dosing and odor abatement system. Therefore the entire process control operation is integrated to operate through PLCs with following five modules: i) Water addition module ii) Chemical preparation and dosing system iii) pH monitoring and float recycle system iv) Drum rotation module v) Odor reduction module. In the integrated system, critical and bulk chemicals are stored in bulk storage tanks and are drawn into the load cell (LC) as per process sequence or recipe for feeding into the drums through auxiliary tanks. The float-recycle system helps to remix & heat the float where a pH electrode is housed to monitor pH online. The pH monitoring system adjusts addition of critical chemicals that indicates automatic end point. The contaminated air inside the tannery is sucked and passed through blower and then through bio-filter. The filtration process is based on the principle that VOCs (in the order of 50-200 ppm) and odors can be biologically treated by naturally occurring microbes. The control parameters monitored are: moisture in the bed and uniformity of media (contaminated air or process liquor). The humidity and temperature of inlet media is controlled and contact time with microbes is 10-30 secs. Moisture is controlled to maintain microbial population. A lead in laboratory scale has been developed to measure process variables (PV) considering their spatial distribution in two dimensions. Spatial distribution of process variables inside hides (across cross section) may provide accurate measurement of through reconstruction of image and data driven models using artificial intelligence tools. Computational intelligence is developed for updation of model parameters as that can be used for direct estimation of PV Take-Away: 1. Cleaner production is provided through automation of dosing & pH monitoring using PLC in indegeneous way 2. Pollution Load in exit stream and odor-gas emmision are minimized 3. Artificial Intelligence and data analytics techniques are used in Leather makin

CP asymmetry in B→ϕKSB \to \phi K_S in a general two-Higgs-doublet model with fourth-generation quarks

We discuss the time-dependent CP asymmetry of decay $B \to \phi K_S$ in an extension of the Standard Model with both two Higgs doublets and additional fourth-generation quarks. We show that although the Standard Model with two-Higgs-doublet and the Standard model with fourth generation quarks alone are not likely to largely change the effective $\sin 2 \beta$ from the decay of $B \to \phi K_S$, the model with both additional Higgs doublet and fourth-generation quarks can easily account for the possible large negative value of $\sin 2 \beta$ without conflicting with other experimental constraints. In this model, additional large CP violating effects may arise from the flavor changing Yukawa interactions between neutral Higgs bosons and the heavy fourth generation down type quark, which can modify the QCD penguin contributions. With the constraints obtained from $b \to s \bar{s} s$ processes such as $B \to X_s \gamma$ and $\Delta m_{B_s^0}$, this model can lead to the effective $\sin 2 \beta$ to be as large as $- 0.4$ in the CP asymmetry of $B \to \phi K_S$.Comment: 13 pages, 5 figures, references added, to appear in Eur.Phys.J.

Evidence for an Excess of Soft Photons in Hadronic Decays of Z^0

Soft photons inside hadronic jets converted in front of the DELPHI main tracker (TPC) in events of qqbar disintegrations of the Z^0 were studied in the kinematic range 0.2 < E_gamma < 1 GeV and transverse momentum with respect to the closest jet direction p_T < 80 MeV/c. A clear excess of photons in the experimental data as compared to the Monte Carlo predictions is observed. This excess (uncorrected for the photon detection efficiency) is (1.17 +/- 0.06 +/- 0.27) x 10^{-3} gamma/jet in the specified kinematic region, while the expected level of the inner hadronic bremsstrahlung (which is not included in the Monte Carlo) is (0.340 +/- 0.001 +/- 0.038) x 10^{-3} gamma/jet. The ratio of the excess to the predicted bremsstrahlung rate is then (3.4 +/- 0.2 +/- 0.8), which is similar in strength to the anomalous soft photon signal observed in fixed target experiments with hadronic beams.Comment: 37 pages, 9 figures, Accepted by Eur. Phys. J.

Search for composite and exotic fermions at LEP 2

A search for unstable heavy fermions with the DELPHI detector at LEP is reported. Sequential and non-canonical leptons, as well as excited leptons and quarks, are considered. The data analysed correspond to an integrated luminosity of about 48 pb^{-1} at an e^+e^- centre-of-mass energy of 183 GeV and about 20 pb^{-1} equally shared between the centre-of-mass energies of 172 GeV and 161 GeV. The search for pair-produced new leptons establishes 95% confidence level mass limits in the region between 70 GeV/c^2 and 90 GeV/c^2, depending on the channel. The search for singly produced excited leptons and quarks establishes upper limits on the ratio of the coupling of the excited fermio

Energy dependence of Cronin momentum in saturation model for p+Ap+A and A+AA+A collisions

We calculate $\sqrt{s}$ dependence of Cronin momentum for $p+A$ and $A+A$ collisions in saturation model. We show that this dependence is consistent with expectation from formula which was obtained using simple dimentional consideration. This can be used to test validity of saturation model (and distinguish among its variants) and measure $x$ dependence of saturation momentum from experimental data.Comment: LaTeX2e, 12 pages, 8 figure

A Precise Measurement of the Tau Lifetime

The tau lepton lifetime has been measured with the e+e- -> tau+tau- events collected by the DELPHI detector at LEP in the years 1991-1995. Three different methods have been exploited, using both one-prong and three-prong tau decay channels. Two measurements have been made using events in which both taus decay to a single charged particle. Combining these measurements gave tau_tau (1 prong) = 291.8 +/- 2.3 (stat) +/- 1.5 (sys) fs. A third measurement using taus which decayed to three charged particles yielded tau_tau (3 prong) = 288.6 +/- 2.4 (stat) +/- 1.3 (sys) fs. These were combined with previous DELPHI results to measure the tau lifetime, using the full LEP1 data sample, to be tau_tau = 290.9 +/- 1.4 (stat) +/- 1.0 (sys) fs.Comment: 27 pages, 7 figure

Search for charginos in e+e- interactions at sqrt(s) = 189 GeV

An update of the searches for charginos and gravitinos is presented, based on a data sample corresponding to the 158 pb^{-1} recorded by the DELPHI detector in 1998, at a centre-of-mass energy of 189 GeV. No evidence for a signal was found. The lower mass limits are 4-5 GeV/c^2 higher than those obtained at a centre-of-mass energy of 183 GeV. The (\mu,M_2) MSSM domain excluded by combining the chargino searches with neutralino searches at the Z resonance implies a limit on the mass of the lightest neutralino which, for a heavy sneutrino, is constrained to be above 31.0 GeV/c^2 for tan(beta) \geq 1.Comment: 22 pages, 8 figure