Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility.

Localization of the N-methyl-D-aspartate type glutamate receptor (NMDAR) to dendritic spines is essential for excitatory synaptic transmission and plasticity. Rather than remaining trapped at synaptic sites, NMDA receptors undergo constant cycling into and out of the postsynaptic density. Receptor movement is constrained by protein-protein interactions with both the intracellular and extracellular domains of the NMDAR. The role of extracellular interactions on the mobility of the NMDAR is poorly understood. Here we demonstrate that the positive surface charge of the hinge region of the N-terminal domain in the GluN1 subunit of the NMDAR is required to maintain NMDARs at dendritic spine synapses and mediates the direct extracellular interaction with a negatively charged phospho-tyrosine on the receptor tyrosine kinase EphB2. Loss of the EphB-NMDAR interaction by either mutating GluN1 or knocking down endogenous EphB2 increases NMDAR mobility. These findings begin to define a mechanism for extracellular interactions mediated by charged domains

SCADA and related technologies

Presented at SCADA and related technologies for irrigation district modernization, II: a USCID water management conference held on June 6-9, 2007 in Denver, Colorado.Northern Water (Northern Colorado Water Conservancy District) conducted field demonstrations and comparisons of flow monitoring equipment at 18 canal and ditch sites in the lower South Platter River Basin during the 2006 irrigation season. Equipment included data loggers from 8 different manufacturers, 16 different models of water level sensors from 12 manufacturers, and 4 different types of telemetry from 7 manufacturers. The data loggers that were demonstrated included four models of single-sensor with integrated data logger, four models of programmable multi-sensor data logger, and one model of basic, low-cost data logger without telemetry. Relative equipment costs for each data logger system are summarized in Table 6. The water level sensors tested included submersible pressure transducers, optical shaft encoders, ultrasonic distance sensors, bubbler level sensor, float and pulley with potentiometer, buoyancy sensor, and a laser distance sensor. Bench checks of sensor calibrations were accomplished by Northern Water staff before field installation, and again at the end of the irrigation season. Observed sensor accuracy was compared to that expected from manufacturer specifications. The telemetry systems tested in the field included license-free spread-spectrum radios from four manufacturers, licensed radio modems in the 450 MHz range, satellite radio modems to a web server, and cdma modems with static IP addresses. Increased mast height and high gain directional antenna improved radio telemetry as expected. Additionally, operational files were utilized to document telemetry performance when available. The purpose and intent of the equipment demonstration and comparison was not to identify a single best data logger, sensor, and/or telemetry system. Each has different features and strengths, as well as varying costs. For each specific flow monitoring application, different equipment may be preferred or better suited than other equipment. However, the 2006 demonstration and comparison should provide a reference point for those seeking to become more knowledgeable in equipment selection while avoiding unpleasant surprises

Late Quaternary uplift along the North America-Caribbean plate boundary: Evidence from the sea level record of Guantanamo Bay, Cuba

The tectonic setting of the North America-Caribbean plate boundary has been studied intensively, but some aspects are still poorly understood, particularly along the Oriente fault zone. Guantanamo Bay, southern Cuba, is considered to be on a coastline that is under a transpressive tectonic regime along this zone, and is hypothesized to have a low uplift rate. We tested this by studying emergent reef terrace deposits around the bay. Reef elevations in the protected, inner part of the bay are ~11e12 m and outercoast, wave-cut benches are as high as ~14 m. Uranium-series analyses of corals yield ages ranging from ~133 ka to ~119 ka, correlating this reef to the peak of the last interglacial period, marine isotope stage (MIS) 5.5. Assuming a span of possible paleo-sea levels at the time of the last interglacial period yields long-term tectonic uplift rates of 0.02e0.11 m/ka, supporting the hypothesis that the tectonic uplift rate is low. Nevertheless, on the eastern and southern coasts of Cuba, east and west of Guantanamo Bay, there are flights of multiple marine terraces, at higher elevations, that could record a higher rate of uplift, implying that Guantanamo Bay may be anomalous. Southern Cuba is considered to have experienced a measurable but modest effect from glacial isostatic adjustment (GIA) processes. Thus, with a low uplift rate, Guantanamo Bay should show no evidence of emergent marine terraces dating to the ~100 ka (MIS 5.3) or ~80 ka (MIS 5.1) sea stands and results of the present study support this

Late Quaternary uplift along the North America-Caribbean plate boundary: Evidence from the sea level record of Guantanamo Bay, Cuba

The tectonic setting of the North America-Caribbean plate boundary has been studied intensively, but some aspects are still poorly understood, particularly along the Oriente fault zone. Guantanamo Bay, southern Cuba, is considered to be on a coastline that is under a transpressive tectonic regime along this zone, and is hypothesized to have a low uplift rate. We tested this by studying emergent reef terrace deposits around the bay. Reef elevations in the protected, inner part of the bay are ~11e12 m and outercoast, wave-cut benches are as high as ~14 m. Uranium-series analyses of corals yield ages ranging from ~133 ka to ~119 ka, correlating this reef to the peak of the last interglacial period, marine isotope stage (MIS) 5.5. Assuming a span of possible paleo-sea levels at the time of the last interglacial period yields long-term tectonic uplift rates of 0.02e0.11 m/ka, supporting the hypothesis that the tectonic uplift rate is low. Nevertheless, on the eastern and southern coasts of Cuba, east and west of Guantanamo Bay, there are flights of multiple marine terraces, at higher elevations, that could record a higher rate of uplift, implying that Guantanamo Bay may be anomalous. Southern Cuba is considered to have experienced a measurable but modest effect from glacial isostatic adjustment (GIA) processes. Thus, with a low uplift rate, Guantanamo Bay should show no evidence of emergent marine terraces dating to the ~100 ka (MIS 5.3) or ~80 ka (MIS 5.1) sea stands and results of the present study support this

Sea-level records at ~80 ka from tectonically stable platforms: Florida and Bermuda

Studies from tectonically active coasts on New Guinea and Barbados have suggested that sea level at ~80 ka was significantly lower than present, whereas data from the Atlantic and Pacific coasts of North America indicate an ~80 ka sea level close to that of the present. We determined ages of corals from a shallow submerged reef off the Florida Keys and an emergent marine deposit on Bermuda. Both localities are on tectonically stable platforms distant from plate boundaries. Uranium-series ages show that corals at both localities grew during the ~80 ka sea-level highstand, and geologic data show that sea level at that time was no lower than 7–9 m below present (Florida) and may have been 1–2 m above present (Bermuda). The ice-volume discrepancy of the 80 ka sea-level estimates is greater than the volume of the Greenland or West Antarctic ice sheets. Comparison of our ages with high-latitude insolation values indicates that the sea-level stand near the present at ~80 ka could have been orbitally forced

Preparation and In Vitro Release of Drug-Loaded Microparticlesfor Oral Delivery Using Wholegrain Sorghum Kafirin Protein

Kafirin microparticles have been proposed as an oral nutraceutical and drug delivery system. This study investigates microparticles formed with kafirin extracted from white and raw versus cooked red sorghum grains as an oral delivery system. Targeted delivery to the colon would be beneficial for medication such as prednisolone, which is used in the management of inflammatory bowel disease. Therefore, prednisolone was loaded into microparticles of kafirin from the different sources using phase separation. Differences were observed in the protein content, in vitro protein digestibility, and protein electrophoretic profile of the various sources of sorghum grains, kafirin extracts, and kafirin microparticles. For all of the formulations, the majority of the loaded prednisolone was not released in in vitro conditions simulating the upper gastrointestinal tract, indicating that most of the encapsulated drug could reach the target area of the lower gastrointestinal tract. This suggests that these kafirin microparticles may have potential as a colon-targeted nutraceutical and drug delivery system