10 research outputs found

    Solvent-selective routing for centrifugally automated solid-phase purification of RNA

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    The final publication is available at Springer via https://doi.org/10.1007/s10404-014-1477-9.We present a disc-based module for rotationally controlled solid-phase purification of RNA from cell lysate. To this end, multi-stage routing of a sequence of aqueous and organic liquids into designated waste and elution reservoirs is implemented by a network of strategically placed, solvent-selective composite valves. Using a bead-based stationary phase at the entrance of the router, we show that total RNA is purified with high integrity from cultured MCF7 and T47D cell lines, human leucocytes and Haemophilus influenzae cell lysates. Furthermore, we demonstrate the broad applicability of the device through the in vitro amplification of RNA purified on-disc using RT-PCR and NASBA. Our novel router will be at the pivot of a forthcoming, fully integrated and automated sample preparation system for RNA-based analysis.Peer reviewe

    Centrifugal Flow Control

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    Sharkeye: Real-Time Autonomous Personal Shark Alerting via Aerial Surveillance

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    While aerial shark spotting has been a standard practice for beach safety for decades, new technologies offer enhanced opportunities, ranging from drones/unmanned aerial vehicles (UAVs) that provide new viewing capabilities, to new apps that provide beachgoers with up-to-date risk analysis before entering the water. This report describes the Sharkeye platform, a first-of-its-kind project to demonstrate personal shark alerting for beachgoers in the water and on land, leveraging innovative UAV image collection, cloud-hosted machine learning detection algorithms, and reporting via smart wearables. To execute, our team developed a novel detection algorithm trained via machine learning based on aerial footage of real sharks and rays collected at local beaches, hosted and deployed the algorithm in the cloud, and integrated push alerts to beachgoers in the water via a shark app to run on smartwatches. The project was successfully trialed in the field in Kiama, Australia, with over 350 detection events recorded, followed by the alerting of multiple smartwatches simultaneously both on land and in the water, and with analysis capable of detecting shark analogues, rays, and surfers in average beach conditions, and all based on ~1 h of training data in total. Additional demonstrations showed potential of the system to enable lifeguard-swimmer communication, and the ability to create a network on demand to enable the platform. Our system was developed to provide swimmers and surfers with immediate information via smart apps, empowering lifeguards/lifesavers and beachgoers to prevent unwanted encounters with wildlife before it happens

    The use of polybutene for controlling the flow of liquids in centrifugal microfluidic systems

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    The field of centrifugal microfluidics has evolved over the last several decades to allow implementation of complex biological and chemical assays on Lab-on-Disc (LOD) platforms. Present study describes the use of polymer polybutene for tuning hydrophobic siphons and for liquid volume definition on a centrifugal microfluidic platform. Both the siphon tuning and the volume definition steps are carried out by generating negative pressure that results from the volume expansion caused by the transfer of polybutene from a dedicated chamber into a secondary reservoir via a connecting siphon. The hydrophobic valve of the chamber that holds polybutene bursts open at specific angular velocities that depend on the height and density of the liquid column. Thus, the parameters of siphon activation can be adjusted by tuning the burst angular velocity of the valve that is driven by filling the tuning reservoir with a specific volume of polybutene. The same disc construction can be utilized to provide volume definition functionality to transfer liquids from one reservoir to another reservoir in as many fractions as there are immiscible liquids of different densities in the tuning chamber. The presented work also demonstrates the use of polybutene in sealing fluidic chambers to improve heating efficiency and to minimize evaporation during thermal cycling required for applications such as PCR amplification. Finally, the use of polybutene as a stationary liquid phase in droplet production on a spinning disc is demonstrated.close0

    Centrifugal microfluidic platforms: advanced unit operations and applications

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    Do vardenafil and tadalafil have advantages over sildenafil in the treatment of erectile dysfunction?

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    Erectile dysfunction (ED) affects up to 50% of men between the ages of 40 and 70 years of age. Sildenafil, vardenafil and tadalafil have all been shown to be similarly effective in the treatment of men with ED of vary etiologies, to have similar adverse effects profiles, and to improve quality-of-life by similar amounts. As these phosphodiesterase 5 (PDE5) inhibitors all increase the hypotensive effects of nitrates, they are not suitable for use in patients taking nitrates for the treatment of ischaemic heart disease. All three inhibitors must be used with caution in patients taking alpha(1)-adrenoceptors antagonists for benign prostatic hyperplasia. Although nonarteritic anterior ischaemic neuropathy has been reported in some users of the PDE5 inhibitors, there is no conclusive evidence that PDE5 inhibitors cause this rare effect. Tadalafil has a longer half-life than sildenafil or vardenafil, and a longer duration of action than sildenafil and vardenafil. Most preference studies have shown tadalafil to be preferred, but there are serious limitations to some of these studies. One approach to treatment is to give each patient a short- and long-acting agent, and for individuals to decide their preference
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