Detection of equine atypical myopathy-associated hypoglycin A in plant material: Optimisation and validation of a novel LC-MS based method without derivatisation

Hypoglycin A (HGA) toxicity, following ingestion of material from certain plants, is linked to an acquired multiple acyl-CoA dehydrogenase deficiency known as atypical myopathy, a commonly fatal form of equine rhabdomyolysis seen worldwide. Whilst some plants are known to contain this toxin, little is known about its function or the mechanisms that lead to varied HGA concentrations between plants. Consequently, reliable tools to detect this amino acid in plant samples are needed. Analytical methods for HGA detection have previously been validated for the food industry, however, these techniques rely on chemical derivatisation to obtain accurate results at low HGA concentrations. In this work, we describe and validate a novel method, without need for chemical derivatisation (accuracy = 84–94%; precision = 3–16%; reproducibility = 3–6%; mean linear range R2 = 0.999). The current limit of quantitation for HGA in plant material was halved (from 1μg/g in previous studies) to 0.5μg/g. The method was tested in Acer pseudoplatanus material and other tree and plant species. We confirm that A. pseudoplatanus is most likely the only source of HGA in trees found within European pastures

General Non-equilibrium Theory of Colloid Dynamics

A non-equilibrium extension of Onsager's canonical theory of thermal fluctuations is employed to derive a self-consistent theory for the description of the statistical properties of the instantaneous local concentration profile n(r,t) of a colloidal liquid in terms of the coupled time evolution equations of its mean value n(r,t) and of the covariance {\sigma}(r,r';t) \equiv of its fluctuations {\delta}n(r, t) = n(r, t) - n(r, t). These two coarse-grained equations involve a local mobility function b(r, t) which, in its turn, is written in terms of the memory function of the two-time correlation function C(r, r' ; t, t') \equiv <{\delta}n(r, t){\delta}n(r',t')>. For given effective interactions between colloidal particles and applied external fields, the resulting self-consistent theory is aimed at describing the evolution of a strongly correlated colloidal liquid from an initial state with arbitrary mean and covariance n^0(r) and {\sigma}^0(r,r') towards its equilibrium state characterized by the equilibrium local concentration profile n^(eq)(r) and equilibrium covariance {\sigma}^(eq)(r,r'). This theory also provides a general theoretical framework to describe irreversible processes associated with dynamic arrest transitions, such as aging, and the effects of spatial heterogeneities

Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection.

Tuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active TB patients and their household contacts who were either infected (LTBI) or uninfected (EMI-TB Discovery Cohort, Pontevedra Region, Spain). A TMT (Tamdem mass tags) 10plex-based quantitative proteomics study was performed in quintuplicate containing a total of 15 individual serum samples per group. Peptides were analyzed in an LC-Orbitrap Elite platform, and raw data were processed using Proteome Discoverer 2.1. A total of 418 proteins were quantified. The specific protein signature of active TB patients was characterized by an accumulation of proteins related to complement activation, inflammation and modulation of immune response and also by a decrease of a small subset of proteins, including apolipoprotein A and serotransferrin, indicating the importance of lipid transport and iron assimilation in the progression of the disease. This signature was verified by the targeted measurement of selected candidates in a second cohort (EMI-TB Verification Cohort, Maputo Region, Mozambique) by ELISA and nephelometry techniques. These findings will aid our understanding of the complex metabolic processes associated with TB progression from LTBI to active disease

Validation of a Net Active Debris Removal Simulator within Parabolic Flight Experiment

Currently space debris is recognized as a major risk for space missions. In this frame it is involved the Patender project (Net parametric characterization and parabolic flight). The goal of this ESA funded activity (ending by March 2014) is to develop a confident mean to further investigate, develop and validate the concept of using nets for actively removing space debris of different characteristics. The net simulator will be validated in a parabolic flight experiment where microgravity conditions can be reached during some few tens of seconds. Different net shapes (pyramidal/planar) will be launched using a pneumatic-based dedicated mechanism in order to simulate the capture of a large space debris. High-speed motion cameras will record the experiment in order to allow the 3D reconstruction of the deployment and wrapping around the target phases and the validation of the software simulator

Promoting higher added value to a finfish species rejected to sea

332 páginas.-- José Ramón Fuertes ... et al.This project aimed to the development of the research and the technology necessary to promote higher added value to fishing activity. This is to be achieved by obtaining profit from a finfish species (“Rockcod”, Patagonotothen spp.) not known to consumers and currently discarded by the EU fishing fleet operating in the South West Atlantic, in order to supply the EU seafood industry with a good quality raw material for human food manufacturing. Use of this species, caught as a by-catch in the existing fisheries targeting hakes and cephalopods, should also increase the profitability of the fleet, contribute to maintaining employment and help to counterbalance the negative effects of fishing activity and discards in the ecosystem. The main scientific-technological objectives and expected achievements were the following: - Description of the fisheries - Improved knowledge of the biology of the species - Biomass assessment - Estimation of catches and discards - Analysis of the spatial and temporal distribution of the resource. Fishery forecasting and testing - Sensorial, Microbiological, Nutritional and Biochemical Evaluation of Rock cod - Development of the technical modifications on board commercial vessels - Development of new processed products from frozen Rock codContract number Q5CR-2002-71709Peer reviewe

Self-care and therapeutic education in hypertension. An integrative review

Objetivo: El objetivo de este estudio fue revisar la literatura reciente relacionada a la modificación de hábitos de vida relacionados con la alimentación o el ejercicio físico para conseguir una mejor evolución de la enfermedad y conocer las mejores medidas para permitir una elevada tasa de cumplimiento de las recomendaciones. Método: Se realizó una búsqueda bibliográfica en las bases de datos PudMed, Lilacs, Scielo y Enfispo en Marzo de 2013. El límite temporal fue de 5 años. Se buscaron las palabras clave en título, abstract y como descriptores. Resultados: Se obtuvieron un total de 1050 artículos, de los cuales 978 (93%) fueron excluidos. El principal motivo de exclusión fue el año de publicación, anterior a 2008. PubMed fue la base de datos con mayor número de artículos, seguida de Lilacs. Los artículos de carácter descriptivo fueron los más predominantes con un total de 35 artículos recuperados (56.45%). Conclusiones: La dieta DASH se ofrece como una posible alternativa para el desarrollo de intervenciones relacionadas con la alimentación en pacientes hipertensos. Respecto al ejercicio físico, el programa CIMEQ parece que muestra beneficios en el control de las cifras de TA. No se han encontrado estrategias solidas que garanticen la adherencia a las recomendaciones terapéuticas relacionadas con el ejercicio físico y la alimentación. Parece necesario desarrollar investigaciones de calidad que ayuden a conocer cuáles son las mejores estrategias en educación terapéutica en pacientes con hipertensión.Objectives: The aim of this study was to review the recent literature related to the modification of lifestyle habits related to diet or exercise to get a better evolution of the disease and to know the best measures to allow a high rate of compliance with the recommendations. Method: A bibliographical search was realized in the databases PudMed, Lilacs, Scielo and Enfispo in March, 2013.The temporary limit was 5 years. The key words were looked up in title, abstract and as describers. Results: There were obtained a total of 1050 articles, from which 978 (93%) were excluded. The main motive of exclusion was the year of publication, previous of 2008. Pubmed was the database with major number of articles, followed by Lilacs. The articles of descriptive character were the most predominant with a total of 35articles recovered (56.45%). Conclusions: The DASH diet offers as a possible alternative for the development of interventions related to the feeding in hypertense patients. With regard to the physical exercise, the program CIMEQ seems that it shows benefits in the control of the numbers of TA. Solid strategies that guarantee the adherence to the therapeutic recommendations related to the physical exercise and the feeding were not found. It seems to be necessary to develop quality researches that help to know which are the best strategies in therapeutic education in patients with hypertension

Serendipitous discovery of RR Lyrae stars in the Leo V ultra-faint galaxy

During the analysis of RR Lyrae stars discovered in the High cadence Transient Survey (HiTS) taken with the Dark Energy Camera at the 4-m telescope at Cerro Tololo Inter-American Observatory, we found a group of three very distant, fundamental mode pulsator RR Lyrae (type ab). The location of these stars agrees with them belonging to the Leo V ultra-faint satellite galaxy, for which no variable stars have been reported to date. The heliocentric distance derived for Leo V based on these stars is 173 +/- 5 kpc. The pulsational properties (amplitudes and periods) of these stars locate them within the locus of the Oosterhoff II group, similar to most other ultra-faint galaxies with known RR Lyrae stars. This serendipitous discovery shows that distant RR Lyrae stars may be used to search for unknown faint stellar systems in the outskirts of the Milky Way.Comment: Accepted in ApJ Letter