73 research outputs found

    Sequence analysis of the second internal Transcribed spacer (ITS2) region of rDNA for species identification of trichostrongylus nematodes isolated from domestic livestock in Iran

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    Background: Infectivity of herbivores with Trichostrongylus nematodes is widespread in many countries, having a major economic impact on breeding, survivability, and productivity of domestic livestock. This study was carried out on Trichostrongylus species isolated from domestic livestock in order to develop an easy-to-perform method for species identification. Methods: Trichostrongylus isolates were collected from sheep, goat, cattle, and buffaloes in Khuzestan Province, southwest Iran. Primary species identification was carried out based on morphological characterization of male worms. PCR amplification of ITS2-rDNA region was performed on genomic DNA and the products were sequenced. Phylogenetic analysis of the nucleotide sequence data was conducted employing Bayesian Inference approach. Consequently, a restriction fragment length polymorphism (RFLP) profile was designed to differentiate Trichostrongylus species. Results: A consensus sequence of 238 nucleotides was deposited in the GenBank for Iranian isolates of Trichostrongylus species including T. colubriformis, T. capricola, T. probolurus and T. vitrinus. The designated RFLP using restriction enzyme TasI could readily differentiate among species having different ITS2 sequence. The molecular analysis was in concordance with morphological findings. Conclusion: Phylogenetic analysis indicated a close relationship among the sequences obtained in this study and reference sequence of relevant species. ITS2-RFLP with TasI is recommended for molecular differentiation of common Trichostrongylus species

    Correction to: Isolated Human and Livestock Echinococcus granulosus Genotypes Using Real-Time PCR of cox1 Gene in Northeast Iran (Acta Parasitologica, (2019), 64, 3, (679-685), 10.2478/s11686-019-00117-w)

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    Unfortunately, the affiliation of Majid Fasihi-Harandi needs to be edited. The correct affiliation is Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

    Development of Photonic Crystal Fiber Based Gas/ Chemical Sensors

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    The development of highly-sensitive and miniaturized sensors that capable of real-time analytes detection is highly desirable. Nowadays, toxic or colorless gas detection, air pollution monitoring, harmful chemical, pressure, strain, humidity, and temperature sensors based on photonic crystal fiber (PCF) are increasing rapidly due to its compact structure, fast response and efficient light controlling capabilities. The propagating light through the PCF can be controlled by varying the structural parameters and core-cladding materials, as a result, evanescent field can be enhanced significantly which is the main component of the PCF based gas/chemical sensors. The aim of this chapter is to (1) describe the principle operation of PCF based gas/ chemical sensors, (2) discuss the important PCF properties for optical sensors, (3) extensively discuss the different types of microstructured optical fiber based gas/ chemical sensors, (4) study the effects of different core-cladding shapes, and fiber background materials on sensing performance, and (5) highlight the main challenges of PCF based gas/ chemical sensors and possible solutions

    Antidiabetic effect of actinodaphne angustifolia and profiling of bioactive metabolites using UPLC-QToF/ESI-MS method

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    Diabetes mellitus is a chronic disorder that causes elevated blood glucose levels due to a lack of insulin, either completely or partially. We investigated the antidiabetic property of Actinodaphne angustifolia in a rat model and identified the bioactive phytochemicals by using the UPLC-QTOF/ESI-MS method. The rats’ pancreatic structures, lipid profile and blood glucose were assessed after a one-week intervention. UPLC-QTOF/ESI-MS analysis was conducted to identify flavonoids and terpenoids in the leaf extract. Actinodaphne angustifolia extract markedly increased the high-density lipoprotein (HDL) (p<0.05) while reducing total cholesterol (TC), low-density lipoprotein (LDL) and blood glucose. Furthermore, the tissue architecture of pancreatic islets was also well recovered as compared to the control group. A total of 45 flavonoids and 109 terpenoid compounds were identified using UPLC-QTOF/ESI-MS-based analysis and additional studies should be undertaken to identify the potential antidiabetic agents

    Antidiabetic Effect of Actinodaphne angustifolia and Profiling of Bioactive Metabolites using UPLC-QToF/ESI-MS Method

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    Diabetes mellitus adalah gangguan kronik yang menyebabkan paras glukosa darah meningkat akibat kekurangan insulin, sama ada sepenuhnya atau sebahagian. Kami menyiasat sifat antidiabetik Actinodaphne angustifolia dalam model tikus dan mengenal pasti fitokimia bioaktif dengan menggunakan kaedah UPLC-QTOF/ ESI-MS. Struktur pankreas tikus, profil lipid dan glukosa darah dinilai selepas intervensi selama satu minggu. Analisis UPLC-QTOF/ESI-MS telah dijalankan untuk mengenal pasti flavonoid dan terpenoid dalam ekstrak daun. Ekstrak Actinodaphne angustifolia dengan ketara meningkatkan lipoprotein berketumpatan tinggi (HDL) (p<0.05) sambil mengurangkan jumlah kolesterol (TC), lipoprotein berketumpatan rendah (LDL) dan glukosa darah. Tambahan pula, struktur seni tisu pulau pankreas juga pulih dengan baik berbanding kumpulan kawalan. Sebanyak 45 flavonoid dan 109 sebatian terpenoid telah dikenal pasti menggunakan analisis berasaskan UPLC-QTOF/ESI-MS dan kajian tambahan perlu dijalankan untuk mengenal pasti agen antidiabetik yang berpotensi. Diabetes mellitus is a chronic disorder that causes elevated blood glucose levels due to a lack of insulin, either completely or partially. We investigated the antidiabetic property of Actinodaphne angustifolia in a rat model and identified the bioactive phytochemicals by using the UPLC-QTOF/ESI-MS method. The rats’ pancreatic structures, lipid profile and blood glucose were assessed after a one-week intervention. UPLC-QTOF/ESI-MS analysis was conducted to identify flavonoids and terpenoids in the leaf extract. Actinodaphne angustifolia extract markedly increased the high-density lipoprotein (HDL) (p<0.05) while reducing total cholesterol (TC), low-density lipoprotein (LDL) and blood glucose. Furthermore, the tissue architecture of pancreatic islets was also well recovered as compared to the control group. A total of 45 flavonoids and 109 terpenoid compounds were identified using UPLC-QTOF/ESI-MS-based analysis and additional studies should be undertaken to identify the potential antidiabetic agents

    Spatial Distribution and Molecular Identification of Leishmania Species from Endemic Foci of South-Eastern Iran

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    Background: Cutaneous leishmaniasis constitutes a major public health problem in many parts of the world including Iran. The primary objective of this study was to identify Leishmania species in endemic districts of Kerman Province, south-eastern Iran. Methods: This study was conducted by random sampling as cross- sectional descriptive between 2008 and 2010. Overall, 203 skin scraping smears were taken from the patients. Nested –PCR was performed to amplify variable minicircle fragments of Leishmania kDNA. Results: Bam was the most infected district (71.1%), followed by Kerman (14.7%), Jiroft (5.4%), Baft (2.7%), Sirjan (1.6%), Shahr-e Babak (1.5%) and others (3.0%). L. tropica was the most common species identified (194 cases, 95.6%), while L. major was found in only 9 cases (4.4%). Of 203 identified patients, all species in Bam (l07 cases), Kerman (32 cases), Jiroft (l6 cases) and Shahr-e- Babak (l1 cases) were detected as L. tropica, whereas infected subjects in Baft and Sirjan showed L. tropica or L. major. Characterization of Leishmania species resulted in generation of 750 bp and 560 bp fragments, corresponding to those of L. tropica and L. major, respectively. Conclusion: L. tropica is the main species (95.6%) caused ACL in endemic areas of Kerman Province; however L. major is present in low level (4.4%)

    Suppression of PGE2 production via disruption of MAPK phosphorylation by unsymmetrical dicarbonyl curcumin derivatives

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    Curcumin is an important molecule found in turmeric plants and has been reported to exhibit some profound anti-inflammatory activities by interacting with several important molecular targets found in the mitogen-activated protein kinase and NF-κβ pathways. As part of our continuing effort to search for new anti-inflammatory agents with better in vitro and in vivo efficacies, we have synthesized a series of new unsymmetrical dicarbonyl curcumin derivatives and tested their effects on prostaglandin E2 secretion level in interferon-γ/lipopolysaccharide-activated macrophage cells. Among those, five compounds exhibited remarkable suppression on prostaglandin E2 production with IC50 values ranging from 0.87 to 18.41 µM. The most potent compound 17f was found to down-regulate the expression of cyclooxygenase-2 mRNA suggesting that this series of compounds could possibly target the mitogen-activated protein kinase signal transduction pathway. Whilst the compound did not affect the expression of the conventional mitogen-activated protein kinases, the results suggest that it could disrupt the phosphorylation and activation of the proteins particularly the c-Jun N-terminal kinases. Finally, the binding interactions were examined using the molecular docking and dynamics simulation approaches

    Predicting the environmental suitability for onchocerciasis in Africa as an aid to elimination planning

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    Recent evidence suggests that, in some foci, elimination of onchocerciasis from Africa may be feasible with mass drug administration (MDA) of ivermectin. To achieve continental elimination of transmission, mapping surveys will need to be conducted across all implementation units (IUs) for which endemicity status is currently unknown. Using boosted regression tree models with optimised hyperparameter selection, we estimated environmental suitability for onchocerciasis at the 5 × 5-km resolution across Africa. In order to classify IUs that include locations that are environmentally suitable, we used receiver operating characteristic (ROC) analysis to identify an optimal threshold for suitability concordant with locations where onchocerciasis has been previously detected. This threshold value was then used to classify IUs (more suitable or less suitable) based on the location within the IU with the largest mean prediction. Mean estimates of environmental suitability suggest large areas across West and Central Africa, as well as focal areas of East Africa, are suitable for onchocerciasis transmission, consistent with the presence of current control and elimination of transmission efforts. The ROC analysis identified a mean environmental suitability index of 0.71 as a threshold to classify based on the location with the largest mean prediction within the IU. Of the IUs considered for mapping surveys, 50.2% exceed this threshold for suitability in at least one 5×5-km location. The formidable scale of data collection required to map onchocerciasis endemicity across the African continent presents an opportunity to use spatial data to identify areas likely to be suitable for onchocerciasis transmission. National onchocerciasis elimination programmes may wish to consider prioritising these IUs for mapping surveys as human resources, laboratory capacity, and programmatic schedules may constrain survey implementation, and possibly delaying MDA initiation in areas that would ultimately qualify
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