Planar Crack Approach to Evaluate the Flexural Strength of Fiber-Reinforced Concrete Sections

[Abstract] : This article describes a model based on concepts of Fracture Mechanics to evaluate the flexural strength of fiber-reinforced concrete (FRC) sections. The model covers the need by structural engineers to have tools that allow, in a simple way, the designing of FRC sections and avoiding complex calculations through finite elements. It consists of an analytical method that models FRC post-cracking behavior with a cohesive linear softening law (σ − w). We use a compatibility equation based on the planar crack hypothesis, i.e., the assumption that the crack surfaces remain plane throughout the fracture process, which was recently proven true using digital image correlation. Non-cracked concrete bulk follows a stress–strain law (σ − ε) combined with the Bernoulli–Navier assumption. We define a brittleness number derived from non-dimensional analyses, which includes the beam size and the softening characteristics. We show that this parameter is key to determining the FRC flexural strength, characterizing fiber-reinforced concrete, and reproducing the size-effect of sections in flexure. Moreover, we propose an expression to calculate the flexural strength of FRC as a function of the cited brittleness number. The model also gives the ratio between the residual strength in service conditions and the flexural strength. Model results show a good agreement with tests in the scientific literature. Finally, we also analyze the brittle–ductile transition in FRC sections.Spanish Ministry of Science, Innovation and Universities through grant DIN-2018-009940. Moreover, PID2019-110928RB-C31 and RTC-2017-6736-3 from the same funder, and grant SBPLY/19/180501/000220 from the Junta de Comunidades de Castilla-La Mancha Spain

Global overview of the management of acute cholecystitis during the COVID-19 pandemic (CHOLECOVID study)

Background: This study provides a global overview of the management of patients with acute cholecystitis during the initial phase of the COVID-19 pandemic. Methods: CHOLECOVID is an international, multicentre, observational comparative study of patients admitted to hospital with acute cholecystitis during the COVID-19 pandemic. Data on management were collected for a 2-month study interval coincident with the WHO declaration of the SARS-CoV-2 pandemic and compared with an equivalent pre-pandemic time interval. Mediation analysis examined the influence of SARS-COV-2 infection on 30-day mortality. Results: This study collected data on 9783 patients with acute cholecystitis admitted to 247 hospitals across the world. The pandemic was associated with reduced availability of surgical workforce and operating facilities globally, a significant shift to worse severity of disease, and increased use of conservative management. There was a reduction (both absolute and proportionate) in the number of patients undergoing cholecystectomy from 3095 patients (56.2 per cent) pre-pandemic to 1998 patients (46.2 per cent) during the pandemic but there was no difference in 30-day all-cause mortality after cholecystectomy comparing the pre-pandemic interval with the pandemic (13 patients (0.4 per cent) pre-pandemic to 13 patients (0.6 per cent) pandemic; P = 0.355). In mediation analysis, an admission with acute cholecystitis during the pandemic was associated with a non-significant increased risk of death (OR 1.29, 95 per cent c.i. 0.93 to 1.79, P = 0.121). Conclusion: CHOLECOVID provides a unique overview of the treatment of patients with cholecystitis across the globe during the first months of the SARS-CoV-2 pandemic. The study highlights the need for system resilience in retention of elective surgical activity. Cholecystectomy was associated with a low risk of mortality and deferral of treatment results in an increase in avoidable morbidity that represents the non-COVID cost of this pandemic

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Planar Crack Approach to Evaluate the Flexural Strength of Fiber-Reinforced Concrete Sections

This article describes a model based on concepts of Fracture Mechanics to evaluate the flexural strength of fiber-reinforced concrete (FRC) sections. The model covers the need by structural engineers to have tools that allow, in a simple way, the designing of FRC sections and avoiding complex calculations through finite elements. It consists of an analytical method that models FRC post-cracking behavior with a cohesive linear softening law (&sigma; &minus; w). We use a compatibility equation based on the planar crack hypothesis, i.e., the assumption that the crack surfaces remain plane throughout the fracture process, which was recently proven true using digital image correlation. Non-cracked concrete bulk follows a stress&ndash;strain law (&sigma; &minus; &epsilon;) combined with the Bernoulli&ndash;Navier assumption. We define a brittleness number derived from non-dimensional analyses, which includes the beam size and the softening characteristics. We show that this parameter is key to determining the FRC flexural strength, characterizing fiber-reinforced concrete, and reproducing the size-effect of sections in flexure. Moreover, we propose an expression to calculate the flexural strength of FRC as a function of the cited brittleness number. The model also gives the ratio between the residual strength in service conditions and the flexural strength. Model results show a good agreement with tests in the scientific literature. Finally, we also analyze the brittle&ndash;ductile transition in FRC sections

JORNADAS DE INVESTIGACIÓN DE LA FACULTAD DE INGENIERÍA.JIFI-2006

JIFI 200

Immobilization of lipid substrates: application on phospholipase A2 determination

The purpose of the study was to assess a fluorimetric assay for the determination of total phospholipase A2 (PLA2) activity in biological samples introducing the innovation of immobilized substrates on crosslinked polymeric membranes. The immobilized C12-NBD-PtdCho, a fluorescent analogue of phosphatidylcholine, exhibited excellent stability for 3 months at 4 °C and was not desorbed in the aqueous reaction mixture during analysis. The limit of detection was 0.5 pmol FA (0.2 pg) and the linear part of the response curve extended from 1 up to 190 nmol FA/h/mL sample. The intra- and inter-day relative standard deviations (%RSD), were ≤6 and ≤9 %, respectively. Statistical comparison with other fluorescent methods showed excellent correlation and agreement. Semiempirical calculations showed a fair amount of electrostatic interaction between the NBD-labeled substrate and the crosslinked polyvinyl alcohol with the styryl pyridinium residues (PVA-SbQ) material, from the plane of which, the sn-2 acyl chain of the phospholipid stands out and is accessible by PLA2. Atomic Force Microscopy revealed morphological alterations of the immobilized substrate after the reaction with PLA2. Mass spectrometry showed that only C12-NBD-FA, the PLA2 hydrolysis product, was detected in the reaction mixture, indicating that PLA2 recognizes PVA-SbQ/C12-NBD-PtdCho as a surface to perform catalysis.</p

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.

In 1992 we started assembling an ordered library of cosmid clones from chromosome XIV of the yeast Saccharomyces cerevisiae. At that time, only 49 genes were known to be located on this chromosome and we estimated that 80% to 90% of its genes were yet to be discovered. In 1993, a team of 20 European laboratories began the systematic sequence analysis of chromosome XIV. The completed and intensively checked final sequence of 784,328 base pairs was released in April, 1996. Substantial parts had been published before or had previously been made available on request. The sequence contained 419 known or presumptive protein-coding genes, including two pseudogenes and three retrotransposons, 14 tRNA genes, and three small nuclear RNA genes. For 116 (30%) protein-coding sequences, one or more structural homologues were identified elsewhere in the yeast genome. Half of them belong to duplicated groups of 6-14 loosely linked genes, in most cases with conserved gene order and orientation (relaxed interchromosomal synteny). We have considered the possible evolutionary origins of this unexpected feature of yeast genome organization.Journal ArticleResearch Support, Non-U.S. Gov'tSCOPUS: ar.jinfo:eu-repo/semantics/publishe