Kartlegging av forurensningstilstanden i Meråker gruvefelt

Avrenning fra kisgruvene i Meråkerfeltet påvirker fortsatt vannkvaliteten i vassdragene i nedbørfeltet selv 100 år etter at gruvedriften opphørte. Største forurensningskilde i området er Lillefjell gruve som bidrar med ca. 80 % av kobbertilførslene til Stjørdalselva som er ca. 3 tonn på årsbasis. Gilsåa og Dalåa er mest belastet med tungmetaller, men vassdragsreguleringen som er foretatt, kan ha bidratt til en forverret vannkvalitet i Torsbjørka nedenfor inntaket til kraftverket. Undersøkelser av bunnfaunaen viser klare påvirkninger i Dalåa, mens det var en naturlig bunnfauna i nedre del av Torsbjørka og i Stjørdalselva. Burforsøk med ørret viste en høy dødelighet i Gilsåa/Dalåa. Det ble også påvist at ørreten i Torsbjørka var metallbelastet

Sink i ferskvann - kjemi, tilførsler og biologiske effekter

Det er laget en kunnskapsstatus for betydningen av sink i ferskvann med særlig vekt på tilførsler fra gruveavrenning. I tillegg ble det utført en undersøkelse av biologiske effekter i et vassdrag. Sveselva, med sink-belastning, men få andre metaller. Alle levende organismer har behov for sink, men det er begrensede kunnskaper om mekanismene bak giftigheten til metallet. Effekt-grenser for akvatiske organismer varier fra 10 til over 1000 µg/l. Blant de mest følsomme organismene er enkelte alger, planktoniske krepsdyr og utviklingsstadier hos fisk. I Sveselva var det tilsynelatende effekter på vegetasjon (begroing) allerede ved 20-30 µg/l, men det er usikkert om dette var det reelle belastningsnivået. Både vegetasjon og bunndyr var klart påvirket på stasjoner med konsentrasjoner i området 250-670 µg/l. Kadmium og sink akkumulerte i gjellene til utplassert dammusling på alle stasjoner nedenfor tilførslene og førte til noe forhøyede konsentrasjoner av metallotionein. Dammusling med høye metall-nivåer i gjellene filtrerte mindre alger og hadde høyere respirasjon enn dammusling med lavere metall-nivåer i gjellene. Resultatene viser at det er viktig å benytte flere mål for biologiske effekter i overvåking av effektene av metall-forurensning og å integrere kjemisk og biologisk overvåking

Ecosystem effects of thermal manipulation of a whole lake, Lake Breisjøen, southern Norway (THERMOS project)

International audienceWe conducted a 3-year artificial deepening of the thermocline in the dimictic Lake Breisjøen, southern Norway, by means of a large submerged propeller. An adjacent lake served as untreated reference. The manipulation increased thermocline depth from 6 to 20 m, caused a significant increase in the heat content, and delayed ice-on by about 20 days. There were only minor changes in water chemistry. Concentrations of sulphate declined, perhaps due to greater reduction of sulphate at the sediment-water interface. Concentrations of particulate carbon and nitrogen decreased, perhaps due to increased sedimentation velocity. Water transparency increased. There was no significant change in concentration of phosphorus, the growth-limiting nutrient. There were few significant changes in principal biological components. Phytoplankton biomass and productivity did not change, although the chlorophyll-a concentration showed a small decrease. Phytoplankton species richness increased, and the species composition shifted. Growth of periphyton increased. There was no change in the macrophyte community. The manipulation did not affect the zooplankton biodiversity, but caused a significant shift in the relative abundance (measured as biomass) in the two major copepod species. The manipulation did not affect the individual density, but appeared to have changed the vertical distribution of zoobenthos. Fish populations were not affected. The lake is oligotrophic and clearwater and the manipulation did not change the supply of phosphorus, and thus there were only minor changes in lake chemistry and biology. Effects might be larger in eutrophic and dystrophic lakes in which internal processes are stronger

Nitrate stable isotopes and major ions in snow and ice samples from four Svalbard sites

Increasing reactive nitrogen (N-r) deposition in the Arctic may adversely impact N-limited ecosystems. To investigate atmospheric transport of N-r to Svalbard, Norwegian Arctic, snow and firn samples were collected from glaciers and analysed to define spatial and temporal variations (1 10 years) in major ion concentrations and the stable isotope composition (delta N-15 and delta O-18) of nitrate (NO3-) across the archipelago. The delta N-15(NO3-) and delta O-18(NO3-) averaged -4 parts per thousand and 67 parts per thousand in seasonal snow (2010-11) and -9 parts per thousand and 74 parts per thousand in firn accumulated over the decade 2001-2011. East-west zonal gradients were observed across the archipelago for some major ions (non-sea salt sulphate and magnesium) and also for delta N-15(NO3-) and delta O-18(NO3-) in snow, which suggests a different origin for air masses arriving in different sectors of Svalbard. We propose that snowfall associated with long-distance air mass transport over the Arctic Ocean inherits relatively low delta N-15(NO3-) due to in-transport N isotope fractionation. In contrast, faster air mass transport from the north-west Atlantic or northern Europe results in snowfall with higher delta N-15(NO3-) because in-transport fractionation of N is then time-limited

Relative Abundance of Transcripts (RATs):Identifying differential isoform abundance from RNA-seq [version 1; referees: 1 approved, 2 approved with reservations]

The biological importance of changes in RNA expression is reflected by the wide variety of tools available to characterise these changes from RNA-seq data. Several tools exist for detecting differential transcript isoform usage (DTU) from aligned or assembled RNA-seq data, but few exist for DTU detection from alignment-free RNA-seq quantifications. We present the RATs, an R package that identifies DTU transcriptome-wide directly from transcript abundance estimates. RATs is unique in applying bootstrapping to estimate the reliability of detected DTU events and shows good performance at all replication levels (median false positive fraction < 0.05). We compare RATs to two existing DTU tools, DRIM-Seq & SUPPA2, using two publicly available simulated RNA-seq datasets and a published human RNA-seq dataset, in which 248 genes have been previously identified as displaying significant DTU. RATs with default threshold values on the simulated Human data has a sensitivity of 0.55, a Matthews correlation coefficient of 0.71 and a false discovery rate (FDR) of 0.04, outperforming both other tools. Applying the same thresholds for SUPPA2 results in a higher sensitivity (0.61) but poorer FDR performance (0.33). RATs and DRIM-seq use different methods for measuring DTU effect-sizes complicating the comparison of results between these tools, however, for a likelihood-ratio threshold of 30, DRIM-Seq has similar FDR performance to RATs (0.06), but worse sensitivity (0.47). These differences persist for the simulated drosophila dataset. On the published human RNA-seq dataset the greatest agreement between the tools tested is 53%, observed between RATs and SUPPA2. The bootstrapping quality filter in RATs is responsible for removing the majority of DTU events called by SUPPA2 that are not reported by RATs. All methods, including the previously published qRT-PCR of three of the 248 detected DTU events, were found to be sensitive to annotation differences between Ensembl v60 and v87

Parental health limitations, caregiving and loneliness among women with widowed parents: longitudinal eveidence from France

We investigate how daughters’ feelings of loneliness are impacted when widowed parents develop health limitations, and when daughters take on personal care tasks in response. Using longitudinal data from daughters of widowed parents drawn from the French Family and Intergenerational Relationships Study (ERFI, 1485 observations nested in 557 daughters), we assess (a) whether health limitations of widowed parents are associated with daughters’ feelings of loneliness regardless of whether or not daughters provide personal care and (b) whether there is an effect of care provision on loneliness that cannot be explained by parental health limitations. Fixed effect regression analyses show that widowed parents’ health limitations were associated with raised feelings of loneliness among their daughters. No significant additional effect of providing personal care to a widowed parent was found. Prior research on the impact of health limitations of older parents on the lives of their adult–children has focused mostly on issues related to informal caregiving. Our findings suggest that more attention to the psychosocial impact of parental health limitations—net of actual caregiving—on adult children’s lives is warranted

The Tetraodon nigroviridis reference transcriptome: Developmental transition, length retention and microsynteny of long non-coding RNAs in a compact vertebrate genome

Pufferfish such as fugu and tetraodon carry the smallest genomes among all vertebrates and are ideal for studying genome evolution. However, comparative genomics using these species is hindered by the poor annotation of their genomes. We performed RNA sequencing during key stages of maternal to zygotic transition of Tetraodon nigroviridis and report its first developmental transcriptome. We assembled 61,033 transcripts (23,837 loci) representing 80% of the annotated gene models and 3816 novel coding transcripts from 2667 loci. We demonstrate the similarities of gene expression profiles between pufferfish and zebrafish during maternal to zygotic transition and annotated 1120 long non-coding RNAs (lncRNAs) many of which differentially expressed during development. The promoters for 60% of the assembled transcripts result validated by CAGE-seq. Despite the extreme compaction of the tetraodon genome and the dramatic loss of transposons, the length of lncRNA exons remain comparable to that of other vertebrates and a small set of lncRNAs appears enriched for transposable elements suggesting a selective pressure acting on lncRNAs length and composition. Finally, a set of lncRNAs are microsyntenic between teleost and vertebrates, which indicates potential regulatory interactions between lncRNAs and their flanking coding genes. Our work provides a fundamental molecular resource for vertebrate comparative genomics and embryogenesis studies