Transition to Kindergarten: Parental Efficacy and Experiences During COVID-19

Previous research has highlighted the effectiveness of supporting parents in fostering children’s academic and/or social-emotional development in preparation for kindergarten. Considering the emergence of COVID-19 as a risk factor to which all children and parents have been exposed, it is essential to understand parental mental health as it relates to early childhood development. This study utilized mixed methods to examine the experiences and perspectives of parents (N = 82) whose children were transitioning into kindergarten during the fall of 2020 amidst the COVID-19 pandemic. Results indicate that parents’ knowledge/confidence in managing their child’s transition to kindergarten was inversely associated with their worry during this time and may be a meaningful target for future interventions. Implications regarding how to best support and include parents in early intervention programming prior to kindergarten entry are discussed

Post-traumatic osteoarthritis in mice following mechanical injury to the synovial joint

We investigated the spectrum of lesions characteristic of post-traumatic osteoarthritis (PTOA) across the knee joint in response to mechanical injury. We hypothesized that alteration in knee joint stability in mice reproduces molecular and structural features of PTOA that would suggest potential therapeutic targets in humans. The right knees of eight-week old male mice from two recombinant inbred lines (LGXSM-6 and LGXSM-33) were subjected to axial tibial compression. Three separate loading magnitudes were applied: 6N, 9N, and 12N. Left knees served as non-loaded controls. Mice were sacrificed at 5, 9, 14, 28, and 56 days post-loading and whole knee joint changes were assessed by histology, immunostaining, micro-CT, and magnetic resonance imaging. We observed that tibial compression disrupted joint stability by rupturing the anterior cruciate ligament (except for 6N) and instigated a cascade of temporal and topographical features of PTOA. These features included cartilage extracellular matrix loss without proteoglycan replacement, chondrocyte apoptosis at day 5, synovitis present at day 14, osteophytes, ectopic calcification, and meniscus pathology. These findings provide a plausible model and a whole-joint approach for how joint injury in humans leads to PTOA. Chondrocyte apoptosis, synovitis, and ectopic calcification appear to be targets for potential therapeutic intervention

Controls of Litter Size—Do Conclusions Drawn from Institutional Research Herds Always Have Relevance to Commercial Swine Production?

Increasing litter size in pigs has been an ongoing concern of many producers because it has the greatest impact on profitability of the swine enterprise. To study the biology of conceptus growth and survival, many models have been used by researchers. It was determined that a major component in limiting litter size results from the impacts of limitations in uterine space (i.e. uterine capacity). Placental efficiency, which is the ratio of a fetus’s weight compared with that of its placenta, has been shown to impact litter size, and is heritable. Selection for breeding animals having a high placental efficiency at term, has been shown to increase litter size. Furthermore, although piglet weight was only slightly decreased in offspring of boars and gilts selected for increased placental efficiency, placental size was profoundly reduced. This reduction in placental size was coupled with an increase in vascularity, thus nutrient and oxygen uptake by the conceptus could be accomplished over a decreased surface area of attachment to the uterine wall. Reproductive data obtained to date have been gathered largely from university swine herds that may have little relevance to commercially used US pig breeds. In contrast to the constant evaluations of physiological changes associated with increased litter size at universities, swine seed stock producers have selected for many generations simply on increased litter size and have not bothered to evaluate the resulting physiological changes associated with increased fecundity. Therefore, it was the objective of this study to investigate the reproductive characteristics of a commercially relevant swine herd in Iowa (PIC Camborough Line) at selected gestational ages. Multiparous sows (ranging from 1 to 14 parities) were slaughtered on days 25, 36, and 44 of gestation, time periods corresponding to intervals which are before, during, and after the time when uterine capacity becomes limiting. At the laboratory, the uterine horns were measured and ovulation rate was determined. Conceptuses were removed and fetal and placental weights were determined. Uterine horn length and ovulation rate did not differ between the three gestational groups. Conceptus number decreased from 15.8 ± 0.6 on day 25 to 12.9 ± 0.5 and 12.1 ± 0.4 on day 36 and day 44 (litter size in this population averages ~11.5 liveborn piglets/litter). Conceptus survival to day 25 was 60.2 ± 0.1%, which then decreased to 50.1 ± 0.1% on day 36 and 46.3 ± 0.1% on day 44. There was a positive correlation between conceptus number and ovulation rate on day 25 but by day 36 this association was lost. Conceptus number was not associated with uterine length on day 25, but by day 36 there was a positive association that remained through day 44. On all three gestation days there was a negative association between conceptus number and placental weight, but no association between conceptus number and fetal weight was observed, indicating that larger litters are comprised of conceptuses having small placentae, but the same sized fetuses. These data indicate that, compared with commonly reported values for university herds (16-18 ovulations), ovulation rate in these mixed parity production animals is extremely high, whereas conceptus survival as estimated from the number of conceptuses divided by the number of ovulations was very low. Additionally, although conceptus number was related to the ovulation rate on day 25, by day 36 the limitations of uterine size began to reduce conceptus number irrespective of ovulation rate. These data suggest that ovulation rate is not a limiting factor in litter size in this line of commercially relevant pigs. In contrast, the higher than expected ovulation rate observed in these pigs resulted in significant embryo losses and early uterine crowding. The consequences of this early conceptus crowding may have detrimental impacts on prenatal and postnatal growth rate and survival

Painting: Process

Catalog for the exhibition Painting: Process held at the Seton Hall University Walsh Gallery, January 14 - February 15, 2008. Curated by Jackie Bekiaris and Maria Silvestri. Includes an essay by Jackie Bekiaris and Maria Silvestri. Includes color illustrations

Homing endonuclease I-TevIII: dimerization as a means to a double-strand break

Homing endonucleases are unusual enzymes, capable of recognizing lengthy DNA sequences and cleaving site-specifically within genomes. Many homing endonucleases are encoded within group I introns, and such enzymes promote the mobility reactions of these introns. Phage T4 has three group I introns, within the td, nrdB and nrdD genes. The td and nrdD introns are mobile, whereas the nrdB intron is not. Phage RB3 is a close relative of T4 and has a lengthier nrdB intron. Here, we describe I-TevIII, the H–N–H endonuclease encoded by the RB3 nrdB intron. In contrast to previous reports, we demonstrate that this intron is mobile, and that this mobility is dependent on I-TevIII, which generates 2-nt 3′ extensions. The enzyme has a distinct catalytic domain, which contains the H–N–H motif, and DNA-binding domain, which contains two zinc fingers required for interaction with the DNA substrate. Most importantly, I-TevIII, unlike the H–N–H endonucleases described so far, makes a double-strand break on the DNA homing site by acting as a dimer. Through deletion analysis, the dimerization interface was mapped to the DNA-binding domain. The unusual propensity of I-TevIII to dimerize to achieve cleavage of both DNA strands underscores the versatility of the H–N–H enzyme family

Reliability of dynamic contrast-enhanced magnetic resonance imaging data in primary brain tumours: a comparison of Tofts and shutter speed models

Purpose To investigate the robustness of pharmacokinetic modelling of DCE-MRI brain tumour data and to ascertain reliable perfusion parameters through a model selection process and a stability test. Methods DCE-MRI data of 14 patients with primary brain tumours were analysed using the Tofts model (TM), the extended Tofts model (ETM), the shutter speed model (SSM) and the extended shutter speed model (ESSM). A no-effect model (NEM) was implemented to assess overfitting of data by the other models. For each lesion, the Akaike Information Criteria (AIC) was used to build a 3D model selection map. The variability of each pharmacokinetic parameter extracted from this map was assessed with a noise propagation procedure, resulting in voxel-wise distributions of the coefficient of variation (CV). Results The model selection map over all patients showed NEM had the best fit in 35.5% of voxels, followed by ETM (32%), TM (28.2%), SSM (4.3%) and ESSM (<0.1%). In analysing the reliability of Ktrans, when considering regions with a CV<20%, ≈25% of voxels were found to be stable across all patients. The remaining 75% of voxels were considered unreliable. Conclusions The majority of studies quantifying DCE-MRI data in brain tumours only consider a single model and whole-tumour statistics for the output parameters. Appropriate model selection, considering tissue biology and its effects on blood brain barrier permeability and exchange conditions, together with an analysis on the reliability and stability of the calculated parameters, is critical in processing robust brain tumour DCE-MRI data

Supporting physical activity through co-production in people with severe mental ill health (SPACES): protocol for a randomised controlled feasibility trial.

Background Severe mental ill health (SMI) includes schizophrenia, bipolar disorder and schizoaffective disorder and is associated with premature deaths when compared to people without SMI. Over 70% of those deaths are attributed to preventable health conditions, which have the potential to be positively affected by the adoption of healthy behaviours, such as physical activity. People with SMI are generally less active than those without and face unique barriers to being physically active. Physical activity interventions for those with SMI demonstrate promise, however, there are important questions remaining about the potential feasibility and acceptability of a physical activity intervention embedded within existing NHS pathways. Method This is a two-arm multi-site randomised controlled feasibility trial, assessing the feasibility and acceptability of a co-produced physical activity intervention for a full-scale trial across geographically dispersed NHS mental health trusts in England. Participants will be randomly allocated via block, 1:1 randomisation, into either the intervention arm or the usual care arm. The usual care arm will continue to receive usual care throughout the trial, whilst the intervention arm will receive usual care plus the offer of a weekly, 18-week, physical activity intervention comprising walking and indoor activity sessions and community taster sessions. Another main component of the intervention includes one-to-one support. The primary outcome is to investigate the feasibility and acceptability of the intervention and to scale it up to a full-scale trial, using a short proforma provided to all intervention participants at follow-up, qualitative interviews with approximately 15 intervention participants and 5 interventions delivery staff, and data on intervention uptake, attendance, and attrition. Usual care data will also include recruitment and follow-up retention. Secondary outcome measures include physical activity and sedentary behaviours, body mass index, depression, anxiety, health-related quality of life, healthcare resource use, and adverse events. Outcome measures will be taken at baseline, three, and six-months post randomisation. Discussion This study will determine if the physical activity intervention is feasible and acceptable to both participants receiving the intervention and NHS staff who deliver it. Results will inform the design of a larger randomised controlled trial assessing the clinical and cost effectiveness of the intervention. Trial registration ISRCTN: ISRCTN83877229. Registered on 09.09.2022

Advancing understanding of affect labeling with dynamic causal modeling

Mechanistic understandings of forms of incidental emotion regulation have implications for basic and translational research in the affective sciences. In this study we applied Dynamic Causal Modeling (DCM) for fMRI to a common paradigm of labeling facial affect to elucidate prefrontal to subcortical influences. Four brain regions were used to model affect labeling, including right ventrolateral prefrontal cortex (vlPFC), amygdala and Broca’s area. 64 models were compared, for each of 45 healthy subjects. Family level inference split the model space to a likely driving input and Bayesian Model Selection within the winning family of 32 models revealed a strong pattern of endogenous network connectivity. Modulatory effects of labeling were most prominently observed following Bayesian Model Averaging, with the dampening influence on amygdala originating from Broca’s area but much more strongly from right vlPFC. These results solidify and extend previous correlation and regression-based estimations of negative corticolimbic coupling

Ballet injuries: injury incidence and severity over 1 year.

STUDY DESIGN: Prospective, descriptive single-cohort study. OBJECTIVE: To assess the incidence and severity of injuries to a professional ballet company over 1 year. METHODS: Data for an elite-level ballet company of 52 professional dancers were collected by an in-house medical team using a time-loss injury definition. RESULTS: A total of 355 injuries were recorded, with an overall injury incidence of 4.4 injuries per 1000 hours (female, 4.1; male, 4.8; P>.05) and a mean of 6.8 injuries per dancer (female, 6.3; male, 7.3; P>.05). Mean injury severity was 7 days (female, 4; male, 9; P.05); mean severity of injury was 3 days for females and 9 days for males (P<.05). The percentage of traumatic injuries was 32% for females and 40% for males (P<.05); the corresponding severity was 6 and 10 days, respectively (P<.05). CONCLUSION: The relatively high number of injuries reported and the resulting loss of dance time support the need to introduce interventions to reduce the risk of injury in professional dancers