10 research outputs found
Direct conversion of glycerol into formic acid via water stable Pd(II) catalyzed oxidative carbon–carbon bond cleavage
Conversion of saccharides into formic acid using hydrogen peroxide and a recyclable palladium( ii
Toward Implementation of Quorum Sensing Autoinducers as Biomarkers for Infectious Disease States
The opportunistic bacterial pathogen Pseudomonas
aeruginosa causes chronic lung infections in cystic
fibrosis (CF) patients. Importantly, virulence factor expression and
biofilm formation in P. aeruginosa is
coordinated by quorum sensing (QS) and one of the key QS signaling
molecules is 3-oxo-C<sub>12</sub>-HSL. Remarkably, a tetramic acid,
(C<sub>12</sub>-TA), with antibacterial properties is formed spontaneously
from 3-oxo-C<sub>12</sub>-HSL under physiological conditions. Seeking
to better understand this relationship, we sought to investigate whether
3-oxo-C<sub>12</sub>-HSL and C<sub>12</sub>-TA may be contributing
factors to the overall pathogenicity of P. aeruginosa in CF individuals and if their detection and quantitation in sputum
samples might be used as an indicator to assess disease states and
monitor therapy success in CF patients. To this end, 3-oxo-C<sub>12</sub>-HSL and C<sub>12</sub>-TA concentrations were initially analyzed
in P. aeruginosa flow cell biofilms
using liquid chromatography coupled with mass spectrometry (LC–MS).
A liquid chromatography tandem mass spectrometry (LC–MS/MS)-based
method was then developed and validated for their detection and quantification
in the sputa of CF patients. To the best of our knowledge, this is
the first report to show the presence of both the quorum sensing molecule
(3-oxo-C<sub>12</sub>-HSL) and its rearranged product (C<sub>12</sub>-TA) in human clinical samples such as sputum. A total of 47 sputum
samples from 20 CF and 2 non-CF individuals were analyzed. 3-Oxo-C<sub>12</sub>-HSL was detected and quantified in 45 samples with concentrations
ranging from 20 to >1000 nM; C<sub>12</sub>-TA was found in 14
samples
(13–900 nM). On the basis of our findings, quorum sensing autoinducers
merit further investigation as biomarkers for infectious disease states