Estudio en cadáveres sobre la anatomía de la rama profunda del nervio radial y los cambios que padece en caso de simulación de fracturas proximales del radio

Objetivos: Determinar si existen diferencias en el desplazamiento con los distintos movimientos del antebrazo de la Rama Profunda del Nervio Radial (RPNR) al comparar especímenes intactos y especímenes con una fractura de radio proximal simulada. Metodología: Mediante el abordaje posterolateral de Kocher y siguiendo los planos de disección, identificar la RPNR y tomar las distancias desde el epicóndilo lateral a la RPNR en pronación, posición neutra y supinación con flexión de codo a 90º, y pronación con extensión de codo a 0º, en el total de especímenes. Después, simular una fractura de radio proximal en todos los especímenes, y tomar las mismas medidas con el objetivo de comparar el desplazamiento de la RPNR en un caso anatómico versus fractura. Además, se objetivan una serie de covariables para determinar si modifican la relación entre el desplazamiento de la RPNR y la posición del antebrazo

Evidence for an accretion origin for the outer halo globular cluster system of M31

We use a sample of newly-discovered globular clusters from the Pan-Andromeda Archaeological Survey (PAndAS) in combination with previously-catalogued objects to map the spatial distribution of globular clusters in the M31 halo. At projected radii beyond ~30 kpc, where large coherent stellar streams are readily distinguished in the field, there is a striking correlation between these features and the positions of the globular clusters. Adopting a simple Monte Carlo approach, we test the significance of this association by computing the probability that it could be due to the chance alignment of globular clusters smoothly distributed in the M31 halo. We find the likelihood of this possibility is low, below 1%, and conclude that the observed spatial coincidence between globular clusters and multiple tidal debris streams in the outer halo of M31 reflects a genuine physical association. Our results imply that the majority of the remote globular cluster system of M31 has been assembled as a consequence of the accretion of cluster-bearing satellite galaxies. This constitutes the most direct evidence to date that the outer halo globular cluster populations in some galaxies are largely accreted.Comment: Accepted for publication in ApJ Letter

Second-generation functionalized mediumchain- length polyhydroxyalkanoates: the gateway to high-value bioplastic applications

Polyhydroxyalkanoates (PHAs) are biodegradable biocompatible polyesters, which accumulate as granulesin the cytoplasm of many bacteria under unbalanced growth conditions. Medium-chain-length PHAs (mcl-PHAs), characterizedby C6-C14 branched monomer chains and typically produced by Pseudomonas species, are promising thermoelastomers,as they can be further modified by introducing functional groups in the side chains. Functionalized PHAs areobtained either by feeding structurally related substrates processed through the β-oxidation pathway, or using specificstrains able to transform sugars or glycerol into unsaturated PHA by de novo fatty-acid biosynthesis. Functionalized mcl-PHAs provide modified mechanical and thermal properties, and consequently have new processing requirements andhighly diverse potential applications in emergent fields such as biomedicine. However, process development and sampleavailability are limited due to the toxicity of some precursors and still low productivity, which hinder investigation. Conversely,improved mutant strains designed through systems biology approaches and cofeeding with low-cost substratesmay contribute to the widespread application of these biopolymers. This review focuses on recent developments in theproduction of functionalized mcl-PHAs, placing particular emphasis on strain and bioprocess design for cost-effectiveproduction. [Int Microbiol 2013; 16(1):1-15

Chemical trends in the Galactic halo from APOGEE data

Indexación: Web of Science; Scopus.The galaxy formation process in the A cold dark matter scenario can be constrained from the analysis of stars in the Milky Way's halo system. We examine the variation of chemical abundances in distant halo stars observed by the Apache Point Observatory Galactic Evolution Experiment ( APOGEE), as a function of distance from the Galactic Centre ( r) and iron abundance ([M/H]), in the range 5 less than or similar to r less than or similar to 30 kpc and - 2.5 15 kpc and [M/H] > - 1.1 (larger in the case of O, Mg, and S) with respect to the nearest halo stars. This result confirms previous claims for low-alpha stars found at larger distances. Chemical differences in elements with other nucleosynthetic origins (Ni, K, Na, and Al) are also detected. C and N do not provide reliable information about the interstellar medium from which stars formed because our sample comprises red giant branch and asymptotic giant branch stars and can experience mixing of material to their surfaces.https://academic.oup.com/mnras/article-lookup/doi/10.1093/mnras/stw286

Alkane-grown Beauveria bassiana produce mycelial pellets displaying peroxisome proliferation, oxidative stress, and cell surface alterations

The entomopathogenic fungus Beauveria bassiana is able to grow on insect cuticle18 hydrocarbons as the sole carbon source, inducing several enzymes involved in alkane19 assimilation and concomitantly increasing virulence against insect hosts. In this study, we20 describe some physiological and molecular processes implicated in growth, nutritional21 stress response, and cellular alterations found in alkane-grown fungi. The fungal cytology22 was investigated using light and transmission electron microscopy (TEM) while the surface23 topography was examined using atomic force microscopy (AFM). Fungal hydrophobicity24 was also measured on the cell surface. Additionally, the expression pattern of several genes25 associated with oxidative stress, peroxisome biogenesis, and hydrophobicity were analysed26 by qPCR. We found a novel type of growth in alkane-cultured B. bassiana similar to27 mycelial pellets described in other alkane-free fungi, which were able to germinate and28 produce viable conidia in media without a carbon source and to be pathogenic against29 larvae of the beetles Tenebrio molitor and Tribolium castaneum. Optical microscopy and30 TEM showed that pellets were formed by hyphae cumulates with high peroxidase activity,31 exhibiting peroxisome proliferation and an apparent surface thickening. Alkane-grown32 conidia appeared to be more hydrophobic and cell surfaces displayed different topography33 than glucose-grown cells, as it was observed by AFM. We also found a significant34 induction in several genes encoding for peroxins, catalases, superoxide dismutases, and35 hydrophobins. These results show that both morphological and metabolic changes are36 triggered in mycelial pellets derived from alkane-grown B. bassiana.Fil: Huarte Bonnet, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Santos Da Paixao, Flavia Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Ponce, Juan C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Santana, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Prieto, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentin

Validez estructural de las pruebas de evaluación de realización cognitiva

En este artículo se discuten las contri buciones del Análisis Fa c t o rial Confi rm at o rio a la evaluación de los modelos estru c t u rales de las aptitudes. Se contrasta el ajuste a va rios modelos fa c t o riales altern at ivos de los resultados obtenidos en una mu e s t ra de jóvenes portugueses de enseñanza media y superior (N=849), con doce pru ebas que evalúan tres procesos (comprensión, ra zonamiento y pensamiento dive rgente), mediante ítems de cuat ro contenidos (verbal, fi g u rat ivo ab s t racto, espacial y nu m é rico). Un modelo de seis fa c t o res, que re fleja la importancia conjunta de los procesos cog n i t ivos y de los contenidos, parece el más adecuado. En este contexto, se afi rma que es necesario revisar de nu evo la oposición tradicional ent re modelos jerárquicos y taxonómicos, puesto que ambos tipos son complementarios.Structural Validity of the Provas de Avaliação da Realização Cognitiva. In this article the authors analise the contribution of confirmatory factor analysis in the evaluation of structural models of cognitive abilities. Alternative models are tested in a sample of high school and college students (N=849), in a battery of 12 tests that evaluate three processes (comprehension, reasoning and divergent thinking) considering four itens contents (verbal, figural-abstract, spatial and numerical). A model with six factors, that reflects the variance of the cognitive processes involved and the content of the tasks appears to be the most adjusted. In this context, it is concluded that it may be necessary to re-analyse the traditional dichotomy between hierarchical and taxonomic models of cognitive abilities, once both are seen as complementary

Observational constraints for Lithium depletion before the RGB

Precise Li abundances are determined for 54 giant stars mostly evolving across the Hertzsprung gap. We combine these data with rotational velocity and with information related to the deepening of the convective zone of the stars to analyse their link to Li dilution in the referred spectral region. A sudden decline in Li abundance paralleling the one already established in rotation is quite clear. Following similar results for other stellar luminosity classes and spectral regions, there is no linear relation between Li abundance and rotation, in spite of the fact that most of the fast rotators present high Li content. The effects of convection in driving the Li dilution is also quite clear. Stars with high Li content are mostly those with an undeveloped convective zone, whereas stars with a developed convective zone present clear sign of Li dilution.Comment: 5 pages, 4 figures. accepted for publicatio

Validity of a reading comprehension test for portuguese students

The purpose of this work was to collect construct and criterion-related evidence of validity for a reading comprehension test (TCL - Teste de Compreensão da Leitura) with three vertically scaled forms, designed to assess students from second, third and fourth grade. Method: Two studies were conducted. In the fi rst (n = 1,229), a confi rmatory factor analysis was performed to analyse the test dimensionality. In the second (n= 402), concurrent and predictive evidence of validity was analysed using correlations between TCL, other reading tests and academic achievement. Results: Confi rmatory factor analysis results supported a one-factor structure. Correlation coeffi cients with other reading tests were low to moderate and statistically signifi cant. The TCL forms were shown to be good predictors of students’ reading comprehension as assessed by teachers and of the National Exams of Portuguese Language results. Conclusions: Present results provide empirical evidence for the validity of the TCL forms.Validez de un test de comprensión lectora para alumnos portugueses. Antecedentes: el objetivo de este trabajo fue recoger evidencia de validez de constructo y de criterio para un test de comprensión lectora (TCL- Teste de Compreensão da Leitura) con tres versiones escaladas verticalmente para evaluar alumnos portugueses de segundo, tercero y cuarto cursos de Primaria. Método: se efectuaron dos estudios. En el primero (n= 1,229) se analizó la dimensionalidad de la prueba recurriendo al análisis factorial confi rmatorio. En el segundo (n= 402) se proporcionan datos sobre evidencia de validez concurrente y predictiva, analizando las correlaciones entre los resultados en TCL, los resultados en otras pruebas de lectura y los resultados académicos. Resultados: los análisis factoriales confi rmatorios revelaron que el modelo de un factor se ajusta a los datos. Se obtuvieron coefi cientes de correlación bajos a moderados y estadísticamente signifi cativos entre las puntuaciones en el TCL y en otros tests de lectura. Las puntuaciones en TCL predijeron las puntuaciones obtenidas por los alumnos en los exámenes nacionales de lengua portuguesa y en las competencias de comprensión evaluadas por los maestros. Conclusiones: estos resultados proporcionan evidencia empírica para la validez de las versiones de TCL.This research was supported by Fundação para a Ciência e Tecnologia, grant SFRH/BD/39980/2007 and CIEC – Research Centre on Child Studies, UM (FCT R&D 317

Melatonin treatment decreases c-fos expression in a headache model induced by capsaicin

The aim of the present work was to analyze c-fos response within the trigeminal nucleus caudalis (TNC) of pinealectomized rats and animals that received intraperitoneal melatonin, after intracisternal infusion of capsaicin, used to induce intracranial trigeminovascular stimulation. Experimental groups consisted of animals that received vehicle solution (saline-ethanol-Tween 80, 8:1:1, diluted 1:50) only (VEI, n = 5); animals that received capsaicin solution (200 nM) only (CAP, n = 6); animals submitted to pinealectomy (PX, n = 5); sham-operated animals (SH, n = 5); animals submitted to pinealectomy followed by capsaicin stimulation (200 nM) after 15 days (PX + CAP, n = 7); and animals that received capsaicin solution (200 nM) and intraperitoneal melatonin (10 mg/kg) (CAP + MEL, n = 5). Control rats, receiving vehicle in the cisterna magna, showed a small number of c-fos-positive cells in the TNC (layer I/II) as well as the sham-operated and pinealectomized rats, when compared to animals stimulated by capsaicin. On the other hand, pinealectomized rats, which received capsaicin, presented the highest number of c-fos-positive cells. Animals receiving capsaicin and melatonin treatment had similar expression of the vehicle group. Our data provide experimental evidence to support the role of melatonin and pineal gland in the pathophysiology of neurovascular headaches.Universidade Federal de São Paulo, Escola Paulista Med, Dept Neurol Neurocirurgia, São Paulo, BrazilHosp Israelita Albert Einstein, São Paulo, BrazilUniv São Paulo, Inst Ciencias Biomed, Dept Fisiol Biofis, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Neurol Neurocirurgia, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, São Paulo, BrazilWeb of Scienc

The Metallicity Distribution Functions of SEGUE G and K dwarfs: Constraints for Disk Chemical Evolution and Formation

We present the metallicity distribution function (MDF) for 24,270 G and 16,847 K dwarfs at distances from 0.2 to 2.3 kpc from the Galactic plane, based on spectroscopy from the Sloan Extension for Galactic Understanding and Exploration (SEGUE) survey. This stellar sample is significantly larger in both number and volume than previous spectroscopic analyses, which were limited to the solar vicinity, making it ideal for comparison with local volume-limited samples and Galactic models. For the first time, we have corrected the MDF for the various observational biases introduced by the SEGUE target selection strategy. The SEGUE sample is particularly notable for K dwarfs, which are too faint to examine spectroscopically far from the solar neighborhood. The MDF of both spectral types becomes more metal-poor with increasing |Z|, which reflects the transition from a sample with small [alpha/Fe] values at small heights to one with enhanced [alpha/Fe] above 1 kpc. Comparison of our SEGUE distributions to those of two different Milky Way models reveals that both are more metal-rich than our observed distributions at all heights above the plane. Our unbiased observations of G and K dwarfs provide valuable constraints over the |Z|-height range of the Milky Way disk for chemical and dynamical Galaxy evolution models, previously only calibrated to the solar neighborhood, with particular utility for thin- and thick-disk formation models.Comment: 70 pages, 25 figures, 7 tables. Accepted by The Astrophysical Journa