8 research outputs found
Self-interaction chromatography as a tool for optimizing conditions for membrane protein crystallization
- Author
- Publication venue
- 'International Union of Crystallography (IUCr)'
- Publication date
- 01/01/2010
- Field of study
The second virial coefficient, or B value, is a measurement of how well a protein interacts with itself in solution. These interactions can lead to protein crystallization or precipitation, depending on their strength, with a narrow range of B values (the `crystallization slot') being known to promote crystallization. A convenient method of determining the B value is by self-interaction chromatography. This paper describes how the light-harvesting complex 1-reaction centre core complex from Allochromatium vinosum yielded single straight-edged crystals after iterative cycles of self-interaction chromatography and crystallization. This process allowed the rapid screening of small molecules and detergents as crystallization additives. Here, a description is given of how self-interaction chromatography has been utilized to improve the crystallization conditions of a membrane protein
Direct measurement of protein osmotic second virial cross coefficients by cross-interaction chromatography
- Author
- Publication venue
- Cold Spring Harbor Laboratory Press
- Publication date
- Field of study
The importance of weak protein interactions, such as protein self-association, is widely recognized in a variety of biological and technological processes. Although protein self-association has been studied extensively, much less attention has been devoted to weak protein cross-association, mainly due to the difficulties in measuring weak interactions between different proteins in solution. Here a framework is presented for quantifying the osmotic second virial cross coefficient directly using a modified form of self-interaction chromatography called cross-interaction chromatography. A theoretical relationship is developed between the virial cross coefficient and the chromatographic retention using statistical mechanics. Measurements of bovine serum albumin (BSA)/lysozyme cross-association using cross-interaction chromatography agree well with the few osmometry measurements available in the literature. Lysozyme/α-chymotrypsinogen interactions were also measured over a wide range of solution conditions, and some counterintuitive trends were observed that may provide new insight into the molecular origins of weak protein interactions. The virial cross coefficients presented in this work may also provide insight into separation processes that are influenced by protein cross-interactions, such as crystallization, precipitation, and ultrafiltration
The osmotic pressure of highly concentrated monoclonal antibody solutions: Effect of solution conditions
- Author
- Publication venue
- 'Wiley'
- Publication date
- Field of study
Rapid Measurement of Protein Osmotic Second Virial Coefficients by Self-Interaction Chromatography
- Author
- Abraham M. Lenhoff
- Battistel
- Behlke
- Bonneté
- Cabezas
- Cleland
- Coen
- Connolly
- Connolly
- Curtis
- DePhillips
- Ducruix
- Durbin
- Fagerstam
- Feder
- Fornstedt
- Forsythe
- Freyssinet
- George
- Gold
- Grigsby
- Gripon
- Guo
- Haugland
- Haynes
- Haynes
- Hill
- Hirsch
- Hitscherich
- Kuehner
- Kulkarni
- McQuarrie
- Mukai
- Neal
- Neal
- Oakley
- Patro
- Peter M. Tessier
- Piazza
- Pjura
- Plant
- Porschel
- Ratnayake
- Receveur
- Rosenbaum
- Schaink
- Selo
- Smith
- Sophianopoulos
- Stanley I. Sandler
- StĂĄhlberg
- Sun
- Velev
- Zhang
- Zimm
- Publication venue
- 'Elsevier BV'
- Publication date
- Field of study
ALCOHOL-INDUCED REINFORCEMENT - DOPAMINE AND 5-HT3 RECEPTOR INTERACTIONS IN ANIMALS AND HUMANS
- Author
- Armor
- Armyr
- Barnes
- Bechara
- Blandina
- Blennerhassett
- Blum
- Bolos
- Bond
- Bone
- Buck
- Bunney
- Carboni
- Carr
- Chen
- Clemens
- Cook
- Corbett
- Corbett
- Costall
- Crow
- Davis
- Dewit
- Dietrich
- Dole
- Dolin
- Doty
- Drummond
- Edmonds
- Edwards
- Edwards
- Engel
- Engel
- Ettenberg
- Fadda
- Fratta
- Gallistel
- Gessa
- Gibson
- Gillies
- Glass
- Gorelick
- Grant
- Gudjonsson
- Haraguchi
- Hein
- Higgins
- Hsiao
- Imperato
- Imperato
- Izquidero
- Jarman
- Jasinksi
- Johnson
- Johnson
- Johnson
- Judd
- Karrenbrock
- Kent
- Kitson
- Knapp
- Koob
- Kreek
- Lipnick
- Lister
- Lorr
- Lovinger
- Mackey
- Mayfield
- McNair
- Meert
- Meish
- Mereu
- Merry
- Miliaressis
- Miliaressis
- Miliaressis
- Miliaressis
- Mirin
- Mithani
- Murphy
- Nakajima
- Naranjo
- Nathan
- Nathan
- Noble
- Nuotto
- Oakley
- Orford
- Overton
- Papp
- Parsian
- Paudice
- Peele
- Peters
- Pfeffer
- Phillips
- Phillips
- Pohorecky
- Pollack
- Porschel
- Prado-Alcala
- Prochaska
- Pucilowski
- Rassnick
- Roche
- Rompre
- Rompre
- Ropert
- Rousanville
- Samson
- Samson
- Samson
- Sanchez-Craig
- Saunders
- Schmitt
- Scottish Council on Alcohol
- Secretary of Health and Human Services
- Sellers
- Shefner
- Silva
- Silverstone
- Silverstone
- Skinner
- Smith
- Solomon
- Spryaki
- Spryaki
- Stark
- Stellar
- Stolerman
- Strahlendorf
- Tuck
- Udego
- Valenstein
- Van der Kooy
- Van der Kooy
- Wafford
- Waller
- Waracznyski
- Waraczynski
- Watson
- Weiss
- Willner
- Wise
- World Health Organization
- Wozniak
- Yoemans
- Yoshimoto
- Zarevics
- Publication venue
- Publication date
- 01/01/1993
- Field of study
Alcohol-induced reinforcement: Dopamine and 5-HT3 receptor interactions in animals and humans
- Author
- Armor
- Armyr
- Barnes
- Bechara
- Blandina
- Blennerhassett
- Blum
- Bolos
- Bond
- Bone
- Buck
- Bunney
- Carboni
- Carr
- Chen
- Clemens
- Cook
- Corbett
- Corbett
- Costall
- Crow
- Davis
- Dewit
- Dietrich
- Dole
- Dolin
- Doty
- Drummond
- Edmonds
- Edwards
- Edwards
- Engel
- Engel
- Ettenberg
- Fadda
- Fratta
- Gallistel
- Gessa
- Gibson
- Gillies
- Glass
- Gorelick
- Grant
- Gudjonsson
- Haraguchi
- Hein
- Higgins
- Hsiao
- Imperato
- Imperato
- Izquidero
- Jarman
- Jasinksi
- Johnson
- Johnson
- Johnson
- Judd
- Karrenbrock
- Kent
- Kitson
- Knapp
- Koob
- Kreek
- Lipnick
- Lister
- Lorr
- Lovinger
- Mackey
- Mayfield
- McNair
- Meert
- Meish
- Mereu
- Merry
- Miliaressis
- Miliaressis
- Miliaressis
- Miliaressis
- Mirin
- Mithani
- Murphy
- Nakajima
- Naranjo
- Nathan
- Nathan
- Noble
- Nuotto
- Oakley
- Orford
- Overton
- Papp
- Parsian
- Paudice
- Peele
- Peters
- Pfeffer
- Phillips
- Phillips
- Pohorecky
- Pollack
- Porschel
- Prado-Alcala
- Prochaska
- Pucilowski
- Rassnick
- Roche
- Rompre
- Rompre
- Ropert
- Rousanville
- Samson
- Samson
- Samson
- Sanchez-Craig
- Saunders
- Schmitt
- Scottish Council on Alcohol
- Secretary of Health and Human Services
- Sellers
- Shefner
- Silva
- Silverstone
- Silverstone
- Skinner
- Smith
- Solomon
- Spryaki
- Spryaki
- Stark
- Stellar
- Stolerman
- Strahlendorf
- Tuck
- Udego
- Valenstein
- Van der Kooy
- Van der Kooy
- Wafford
- Waller
- Waracznyski
- Waraczynski
- Watson
- Weiss
- Willner
- Wise
- World Health Organization
- Wozniak
- Yoemans
- Yoshimoto
- Zarevics
- Publication venue
- 'Wiley'
- Publication date
- Field of study
Tests for Anxiolytic Activity
- Author
- A AgĂĽero
- A AgĂĽero
- A Bachy
- A Bane
- A Bilkei-Gorzo
- A Bilkei-Gorzo
- A Bilkei-Gorzo
- A Bourson
- A Butler
- A Cappelli
- A Chagraoui
- A Clarke
- A Contarino
- A Czyrak
- A Célérier
- A Dumuis
- A Fernandez-Guasti
- A Fischer
- A Fleischmann
- A Fletcher
- A Fletcher
- A Frazer
- A Guidotti
- A Hausmann
- A Heest van
- A Holmes
- A Korte
- A Lecci
- A Lecci
- A Lenègre
- A McGregor
- A Newman-Tancredi
- A Newman-Tancredi
- A Pazos
- A Scriabine
- A Shekar
- A Siegel
- A Siegel
- A Siegel
- A Siegel
- A Siegel
- A Wiersma
- A Woods
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- AH Lewin
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- AP Kozinowski
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- APDW Ford
- AR Kooyman
- AR Luft
- AT Barker
- AT Woods-Kettelberger
- AV Anagnostopoulos
- B Arranz
- B Bettler
- B Bjorvatn
- B Costal
- B Costall
- B Costall
- B Costall
- B Costall
- B Costall
- B Czéh
- B Langen
- B LĂĽscher
- B Oliver
- B Olivier
- B Olivier
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- B Olivier
- B Olivier
- B Olivier
- B Olivier
- B Rocha
- B Winter
- BA Cromer
- BA McMillen
- BA McMillen
- BE McKay
- BFX Sobel
- BH Natelson
- BJ Cao
- BJ Jones
- BJ Young
- BK Koe
- BL Jacobs
- BL Roth
- BL Roth
- BM Rabin
- BPH Porschel
- BT Davies
- C Aron
- C Bonnafous
- C Brakebusch
- C Deans
- C Dugovic
- C Gardner
- C Gerald
- C Gross
- C Kleingoor
- C Knott
- C Laurent-Demir
- C Martini
- C Nath
- C Roberts
- C Routledge
- C Sanchez
- C Saucler
- C Sánchez
- C Waeber
- CA Drew
- CA Klepner
- CA Shively
- CC Beaufour
- CD Mahle
- CE Manser
- CH Lee
- CJ Grossman
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- CK Nielsen
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- D Ben-Shachar
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- D Emmanouil
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- E Costa
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- E Drews
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- G Bignami
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- G Bonanno
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- I Artais
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- I Geller
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- K Beaumont
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- L Angelis
- L Cook
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- T Tsutsumi
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- T Yanagita
- T Zyss
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- TH Hijzen
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- TJJ Zelthof
- TJJ Zelthof
- TM DeLorey
- TM Robinson
- TP Blackburn
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- U Rudolph
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Tests for Anxiolytic Activity
- Author
- A AgĂĽero
- A AgĂĽero
- A Bachy
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- A Bali
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- A Bilkei-Gorzo
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- BJ Jones
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- Borsini F Brambilla A, Cesana R, Donetti A
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- Costall B Domeney AM, Naylor RJ, Tyers MB
- Costall B Hendrie CA, Kelly ME, Naylor RJ
- Costall B Kelley ME, Naylor RJ, Onaivi ES
- Costall B Kelly ME, Onaivi ES, Naylor RJ
- Costall B Naylor RJ, Tyers MB
- Costall B Naylor RT, Tyers MB
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- Fleischmann
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- GP Ryan
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- GR Martin
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- Griebel G Simiand J, Steinberg R, Jung M, Gully D, Roger P, Geslin M, Scatton B, Maffrand JP, Soubrié P
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- H Araki
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- H Frielingsdorf
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- H Ito
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- H Möhler
- H Qian
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- J Bormann
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- JB Malick
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- JB Patel
- JB Patel
- JC Bachiega
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- JC Watkins
- JD Weissman
- JE Araujo De
- JE Barrett
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- JI Koenig
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- JV Brady
- JV Selkirk
- JW Bastian
- JW Clitherow
- JW Kebabian
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- K Cambon
- K Chen
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- K Kato
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- K Kudo
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- K Njung’e
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- Keck ME Welt T, Post A, MĂĽller MB, Toschi N, Wigger A, Landgraf R, Holsboer F, Engelmann M
- Kennett GA Wood MD, Bright F, Cilia J, Piper DC, Gager T, Thomas D, Baxter GS, Forbes LT, Ham P, Blackburn TP
- KG Lloyd
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- KHM McAllister
- KJ Stanhope
- KJ Watling
- KL Woodall
- KM Wilcox
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- L Prado de Carvalho
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- L Rydelek-Fitzgerald
- L Segu
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- L Uphouse
- L Vega De la
- L Wischhof
- LA Lebel
- LA Parker
- LA Parker
- LC Iorio
- LC Schenberg
- Leurs R Blandina P, Tedford C, Timmerman H
- LF McNicholas
- LH Conti
- Lippa AS PristiHa A, Nash BA, Greenblatt EN
- LJ Dreshfield
- LJ Steward
- LK Heisler
- LL Melo
- LM Gaster
- Lowry OH Rosebrough NJ, Farr AL, Randall RJ
- LP Baenninger
- LR Fitzpatrick
- LS Araujo
- LS Crnic
- LW Lim
- M Ahern
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- TR Insel
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- Publication venue
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