Tumor collagenase stimulatory factor (TCSF) expression and localization in human lung and breast cancers.

Tumor cell-derived collagenase stimulatory factor (TCSF) stimulates in vitro the biosynthesis of various matrix metalloproteinases involved in tumor invasion, such as interstitial collagenase, gelatinase A, and stromelysin 1. The expression of TCSF mRNAs was studied in vivo, using in situ hybridization and Northern blotting analysis, in seven normal tissues and in 22 squamous cell carcinomas of the lung, and in seven benign proliferations and in 22 ductal carcinomas of the mammary gland. By in situ hybridization, TCSF mRNAs were detected in 40 of 44 carcinomas, in pre-invasive and invasive cancer cells of both lung and breast cancers. TCSF mRNAs and gelatinase A mRNAs were both visualized in the same areas in serial sections in breast cancers, and were expressed by different cells, tumor cells, and fibroblasts. The histological results were confirmed by Northern blot analysis, which showed a higher expression of TCSF mRNAs in cancers than in benign and normal tissues. These observations support the hypothesis that TCSF is an important factor in lung and breast tumor progression

Learning from Sesame Street: A Qualitative Analysis of Acceptance, Inclusion, and Cultural Diversity

Sesame Street first aired on public television in 1968. Sesame Street was developed by television producer Joan Ganz Cooney in partnership with educational and developmental psychologists to bridge the achievement gap in school readiness, specifically in underserved and underrepresented urban communities. This achievement gap was credited to societal racism and a lack of acculturation and racial diversity. Sesame Street was one of the first television programs, and the first children’s program, to feature an urban setting and a culturally diverse cast. During the 1940s, television programming was an almost exclusively white, Caucasian medium. The roles written for minority characters were based primarily on negative racial and cultural stereotypes. Significant research has been conducted examining the influence of Sesame Street on school readiness and Sesame Street’s contribution to cultural inclusion in society, but little research exists examining the lived experiences and changes in the perceptions of children exposed to cultural diversity through Sesame Street. A thematic analysis of participant interview responses revealed consistent, recurring themes, including the normalization of cultural diversity, empathy for others, and a sense of community connectedness. These themes reinforce the tenants of multicultural theory and social learning theory and highlight how Sesame Street normalized diversity and inclusion, which was integrated into the participant’s social understanding. The inclusive and prosocial behaviors modeled by Sesame Street’s cast had a lasting, positive effect on the participant’s social behaviors and attitudes, which they carried into adulthood

Soluble factors regulated by epithelial-mesenchymal transition mediate tumour angiogenesis and myeloid cell recruitment.

peer reviewedEpithelial-to-mesenchymal transition (EMT) programs provide cancer cells with invasive and survival capacities that might favor metastatic dissemination. Whilst signaling cascades triggering EMT have been extensively studied, the impact of EMT on the crosstalk between tumor cells and the tumor microenvironment remains elusive. We aimed to identify EMT-regulated soluble factors that facilitate the recruitment of host cells in the tumor. Our findings indicate that EMT phenotypes relate to the induction of a panel of secreted mediators, namely IL-8, IL-6, sICAM-1, PAI-1 and GM-CSF, and implicate the EMT-transcription factor Snail as a regulator of this process. We further show that EMT-derived soluble factors are pro-angiogenic in vivo (in the mouse ear sponge assay), ex vivo (in the rat aortic ring assay) and in vitro (in a chemotaxis assay). Additionally, conditioned medium from EMT-positive cells stimulates the recruitment of myeloid cells. In a bank of 40 triple-negative breast cancers, tumors presenting features of EMT were significantly more angiogenic and infiltrated by a higher quantity of myeloid cells compared to tumors with little or no EMT. Taken together, our results show that EMT programs trigger the expression of soluble mediators in cancer cells that stimulate angiogenesis and recruit myeloid cells in vivo, which might in turn favor cancer spread

Viability of the implementation of ISO/TC 228/WG 5 for beaches' certification – study case for the Praia Brava, Itajaí, Santa Catarina – Brazil

The standards by which beach management systems are efficiently managed are best achieved through the application of strict guidelines and requirements, such as those that will be necessary to achieve ISO/TC 228/WG 5 certification. Standards such as these will aid in providing quality services for beachgoers. It is possible verify that the requirements of a standard are met by inserting them into an evaluation matrix that is adapted from the Model for Performance Evaluation of Integrated Coastal Management. Thus, we can ascertain the efficiency of the current management system. This paper focused on the perception of 11 institutions that are involved directly in local tourism in one Brazilian beach, Praia Brava, for which the effectiveness of applied management systems was checked. This beach was used as a particular case study for proving the inadequate management of Brazilian coastal environments.The standards by which beach management systems are efficiently managed are best achieved through the application of strict guidelines and requirements, such as those that will be necessary to achieve ISO/TC 228/WG 5 certification. Standards such as these will aid in providing quality services for beachgoers. It is possible verify that the requirements of a standard are met by inserting them into an evaluation matrix that is adapted from the Model for Performance Evaluation of Integrated Coastal Management. Thus, we can ascertain the efficiency of the current management system. This paper focused on the perception of 11 institutions that are involved directly in local tourism in one Brazilian beach, Praia Brava, for which the effectiveness of applied management systems was checked. This beach was used as a particular case study for proving the inadequate management of Brazilian coastal environments.

The Effect of Storage Temperature and Time on The Quality of Spray Dried Egg Powder

Dehydration is a good approach for egg preservation. However, dried egg products may still suffer from deterioration if stored in an abused temperature environment or prolonged times. Storage conditions can induce undesirable reactions and loss of functionality and quality. The objective of this study was to evaluate the physicochemical characteristics and functionality of spray dried egg yolk powders, which included plain whole egg (PLWE), free flow yolk (FFY), enzyme modified yolk (EMY), and free flow whole egg (FFWE) stored at 4 °C, 25 °C, 43 °C or 54 °C, over a 2-month period; and provide useful suggestions to maximize quality when used in the food industry. Functionality and physicochemical tests included solubility index, emulsifying stability, viscosity and heat stability of emulsions, free fatty acid levels, lipid hydro peroxides, color change, and morphological appearance. Solubility Index results indicated that FFWE was the most soluble sample followed by PLWE, EMY, and FFY. Emulsion stability results revealed that EMY formed the most stable emulsion followed by FFWE, FFY, and PLWE. Free fatty acid levels indicated that EMY deteriorated faster, followed by FFWE, FFY, and PLWE. Lipid hydro-peroxides were most abundant in EMY followed by FFY, PLWE, and FFWE. Total color change was highest for FFY, followed by PLWE, EMY, and FFWE. All samples passed the heat test after 1 month at storage but failed after 2 months of storage at all temperatures. The morphology of the egg powder particles revealed that high processing temperatures increased porosity which resulted in greater oxidation and storage time induce agglomeration of the egg powders. Overall, physicochemical changes found during the storage of dried egg powders were more evident at 43 °C or 54 °C. Lipid oxidation was responsible for the decrease in quality of dried egg powders. Therefore, mostly effective approaches are required to prevent oxidation reactions. Adjusting the storage temperature and humidity, and using an effective packaging method with an oxygen barrier is strongly suggested

Fundamental reassessment of the taxonomy of five Normapolles pollen genera

National audienceDiagnoses of the Normapolles pollen genera Hungaropollis,Krutzschipollis, Longanulipollis, Oculopollis and Trudopollis and56 of their species are emended and many specimens illustrated inorder to provide a basis for more successful and consistent identificationthan has been possible hitherto. These taxa were recoveredfrom palynological preparations of selected samples from Late Cretaceousdeposits in Europe, southern Sweden and eastern USA.As indicated on an accompanying range chart the majority are encounteredin Santonian and/or Campanian deposits. Eleven of thespecies described have been transferred from other genera and aretherefore in new combinations, namely: Hungaropollis granulatus(Kedves et Herngreen), Longanulipollis coronatiformis (Góczánet Siegl-Farkas), L. orbicularis (Góczán), L. ornatus (Kedves etDiniz), L. parvoculus (Góczán), L. skarbyae (Kedves et Diniz),Oculopollis artifex (Weyland et Krieger), O. rector (Pflug), O.triceps (Skarby), Trudopollis cuneolis (Góczán et Siegl-Farkas),and T. spinulosus (Skarby). Six are new: Hungaropollis pinguis,Krutzschipollis cucullus, K. immanis, Longanulipollis amabilis, L.lobus and Oculopollis viriosus. Thirteen genera are regarded assynonyms of Hungaropollis (Aveiropollenites and Romeinipollenites),Longanulipollis (Coronatipollis, Intercalaripollis, Portaepollenitesand Verruoculopollis), Oculopollis (Druggipollenites, Pseudoculopollisand Semioculopollis) and Trudopollis (Cuneipollis,Felderipollenites, Hofkeripollenites and Kriegeripollenites) respectively.As a result of some of these nomenclatural changes 14 otherspecies are in new generic combinations but are not otherwise considered.Seven morphotypes are identified in open nomenclatureand four placed in comparison

Analyse de maillages surfaciques par construction et comparaison de modèles moyens et par décomposition par graphes s’appuyant sur les courbures discrètes : application à l’étude de la cornée humaine

Réalisé en cotutelle avec Aix Marseille Université.Cette thèse se découpe en trois parties. Les deux premières portent sur le développement de méthodes pour la construction de modèles géométriques moyens et pour la comparaison de modèles. Ces approches sont appliquées à la cornée humaine pour l’élaboration d’atlas et pour l’étude biométrique robuste. La troisième partie porte sur une méthode générique d'extraction d'informations dans un maillage en s'appuyant sur des propriétés différentielles discrètes afin de construire une structure par graphe permettant l'extraction de caractéristiques par une description sémantique. Les atlas anatomiques conventionnels (papier ou CD-ROM) sont limités par le fait qu'ils montrent généralement l'anatomie d'un seul individu qui ne représente pas nécessairement bien la population dont il est issu. Afin de remédier aux limitations des atlas conventionnels, nous proposons dans la première partie d’élaborer un atlas numérique 3D contenant les caractéristiques moyennes et les variabilités de la morphologie d'un organe, plus particulièrement de la cornée humaine. Plusieurs problématiques sont abordées, telles que la construction d'une cornée moyenne et la comparaison de cornées. Il existe à ce jour peu d'études ayant ces objectifs car la mise en correspondance de surfaces cornéennes est une problématique non triviale. En plus d'aider à développer une meilleure connaissance de l'anatomie cornéenne, la modélisation 3D de la cornée normale permet de détecter tout écart significatif par rapport à la "normale" permettant un diagnostic précoce de pathologies ou anomalies de la forme de la cornée. La seconde partie a pour objectif de développer une méthode pour reconnaître une surface parmi un groupe de surfaces à l’aide de leurs acquisitions 3D respectives, dans le cadre d’une application de biométrie sur la cornée. L’idée est de quantifier la différence entre chaque surface et une surface donnée, et de déterminer un seuil permettant la reconnaissance. Ce seuil est dépendant des variations normales au sein d’un même sujet, et du bruit inhérent à l’acquisition. Les surfaces sont rognées et trouées de façon imprévisible, de plus il n’y a pas de point de mise en correspondance commun aux surfaces. Deux méthodes complémentaires sont proposées. La première consiste à calculer le volume entre les surfaces après avoir effectué un recalage, et à utiliser ce volume comme un critère de similarité. La seconde approche s’appuie sur une décomposition en harmoniques sphériques en utilisant les coefficients comme des descripteurs de forme, qui permettront de comparer deux surfaces. Des résultats sont présentés pour chaque méthode en les comparant à la méthode la plus récemment décrite dans la littérature, les avantages et inconvénients de chacune sont détaillés. Une méthodologie en cascade utilisant ces deux méthodes afin de combiner les avantages de chacune est aussi proposée. La troisième et dernière partie porte sur une nouvelle méthode de décomposition en graphes de maillages 3D triangulés. Nous utilisons des cartes de courbures discrètes comme descripteur de forme afin de découper le maillage traité en huit différentes catégorie de carreaux (ou peak, ridge, saddle ridge, minimal, saddle valley, valley, pit et flat). Ensuite, un graphe d'adjacence est construit avec un nœud pour chaque carreau. Toutes les catégories de carreaux ne pouvant pas être adjacentes dans un contexte continu, des jonctions intermédiaires sont ajoutées afin d'assurer une cohérence continue entre les zones. Ces graphes sont utilisés pour extraire des caractéristiques géométriques décrites par des motifs (ou patterns), ce qui permet de détecter des régions spécifiques dans un modèle 3D, ou des motifs récurrents. Cette méthode de décomposition étant générique, elle peut être appliquée à de nombreux domaines où il est question d’analyser des modèles géométriques, en particulier dans le contexte de la cornée.This thesis comprises three parts. The first two parts concern the development of methods for the construction of mean geometric models and for model comparison. These approaches are applied to the human cornea for the construction of atlases and a robust biometric study. The third part focuses on a generic method for the extraction of information in a mesh. This approach is based on discrete differential properties for building a graph structure to extract features using a semantic description. Conventional anatomical atlases (paper or CD-ROM) are limited by the fact they generally show the anatomy of a single individual who does not necessarily represent the population from which they originate. To address the limitations of conventional atlases, we propose in the first part of this thesis to construct a 3D digital atlas containing the average characteristics and variability of the morphology of an organ, especially that of the human cornea. Several issues are addressed, such as the construction of an average cornea and the comparison of corneas. Currently, there are few studies with these objectives because the matching of corneal surfaces is a non-trivial problem. In addition to help to develop a better understanding of the corneal anatomy, 3D models of normal corneas can be used to detect any significant deviation from the norm, thereby allowing for an early diagnosis of diseases or abnormalities using the shape of the cornea. The second part of this thesis aims to develop a method for recognizing a surface from a group of surfaces using their 3D acquisitions in a biometric application pertinent to the cornea. The concept behind this method is to quantify the difference between each surface and a given surface and to determine the threshold for recognition. This threshold depends on normal variations within the same subject and noise due to the acquisition system. The surfaces are randomly trimmed and pierced ; moreover, there is no common landmark on the surfaces. Two complementary methods are proposed. The first method consists of the computation of the volume between the surfaces after performing geometrical matching and the use of this volume as a criterion of similarity. The second approach is based on a decomposition of the surfaces into spherical harmonics using the coefficients as shape descriptors to compare the two surfaces. Each result of the proposed methods is compared to the most recent method described in the literature, with the benefits and disadvantages of each one described in detail. A cascading methodology using both methods to combine the advantages of each method is also proposed. The third and final part of this thesis focuses on a new method for decomposing 3D triangulated meshes into graphs. We use discrete curvature maps as the shape descriptor to split the mesh in eight different categories (peak, ridge, saddle ridge, minimal, saddle valley, valley, pit and flat). Next, an adjacency graph is built with a node for each patch. Because all categories of patches cannot be adjacent in a continuous context, intermediate junctions are added to ensure the continuous consistency between patches. These graphs are used to extract geometric characteristics described by patterns that allow for the detection of specific regions in a 3D model or recurrent characteristics. This decomposition method, being generic, can be used in many applications to analyze geometric models, especially in the context of the cornea

Change of Measure for Bayesian Field Inversion with Hierarchical Hyperparameters Sampling

This paper proposes an effective treatment of hyperparameters in the Bayesian inference of a scalar field from indirect observations. Obtaining the joint posterior distribution of the field and its hyperparameters is challenging. The infinite dimensionality of the field requires a finite parametrization that usually involves hyperparameters to reflect the limited prior knowledge. In the present work, we consider a Karhunen-Lo{\`e}ve (KL) decomposition for the random field and hyperparameters to account for the lack of prior knowledge of its autocovariance function. The hyperparameters must be inferred. To efficiently sample jointly the KL coordinates of the field and the autocovariance hyperparameters, we introduce a change of measure to reformulate the joint posterior distribution into a hierarchical Bayesian form. The likelihood depends only on the field's coordinates in a fixed KL basis, with a prior conditioned on the hyperparameters. We exploit this structure to derive an efficient Markov Chain Monte Carlo (MCMC) sampling scheme based on an adapted Metropolis-Hasting algorithm. We rely on surrogate models (Polynomial Chaos expansions) of the forward model predictions to further accelerate the MCMC sampling. A first application to a transient diffusion problem shows that our method is consistent with other approaches based on a change of coordinates (Sraj et al., 2016). A second application to a seismic traveltime tomography highlights the importance of inferring the hyperparameters

Phospho-mTOR in non-tumour and tumour bladder urothelium : pattern of expression and impact on urothelial bladder cancer patients

Urothelial bladder carcinoma (UBC) is heterogeneous in its pathology and clinical behaviour. Evaluation of prognostic and predictive biomarkers is necessary, in order to produce personalised treatment options. The present study used immunohistochemistry to evaluate UBC sections containing tumour and non-tumour areas from 76 patients, for the detection of p-mTOR, CD31 and D2-40 (blood and lymphatic vessels identification, respectively). Of the non-tumour and tumour sections, 36 and 20% were scored positive for p-mTOR expression, respectively. Immunoexpression was observed in umbrella cells from non-tumour urothelium, in all cell layers from non-muscle-invasive (NMI) tumours (including expression in superficial cells), and in spots of cells from muscle-invasive (MI) tumours. Positive expression decreased from non-tumour to tumour urothelium, and from pT1/pTis to pT3/pT4 tumours; however, the few pT3/pT4 positive cases had worse survival rates, with 5-year disease-free survival being significantly lower. Angiogenesis occurrence was impaired in pT3/pT4 tumours that did not express p-mTOR. In conclusion, p-mTOR expression in non-tumour umbrella cells is likely a reflection of their metabolic plasticity, and extension to the inner layers of the urothelium in NMI tumours is consistent with an enhanced malignant potential. The expression in cell spots in a few MI tumours and absence of expression in the remaining tumours is intriguing and requires further research. Additional studies regarding the up- and downstream effectors of the mTOR pathway should be conducte