Changes in the secretory profile of NSCLC-associated fibroblasts after ablative radiotherapy: potential impact on angiogenesis and tumor growth

In the context of radiotherapy, collateral effects of ablative ionizing radiation (AIR) on stromal components of tumors remains understudied. In this work, cancer-associated fibroblasts (CAFs) isolated from freshly resected human lung tumors were exposed to AIR (1x18Gy) and analyzed for their release of paracrine factors. Inflammatory mediators and regulators of angiogenesis and tumor growth were analyzed by multiplex protein assays in conditioned medium (CM) from irradiated and non-irradiated CAFs. Additionally, the profile of secreted proteins was examined by proteomics. In functional assays, effects of CAF-CM on proliferative and migratory capacity of lung tumor cells (H-520/H-522) and endothelial cells (HUVECs), and on the tube-forming capacity of endothelial cells was assessed. Our data show that exposure of CAFs to ablative doses of ionizing radiation results in a) down-regulated release of angiogenic factors SDF-1, angiopoietin and thrombospondin-2; b) up-regulated release of growth factor bFGF from most donors, and c) unaffected expression-levels of HGF and inflammatory mediators IL-6, IL-8, IL-1ƒÒ and TNF-£. Conditioned medium from irradiated and control CAFs did not affect differently the proliferative or migratory capacity of tumor cells (H-520/H-522), whereas migratory capacity of endothelial HUVEC cells was partially reduced in the presence of irradiated CAF conditioned medium. Overall we conclude that AIR mediates a transformation on the secretory profile of CAFs that could influence the behavior of other cells in the tumor tissue and hence guide to some extent therapeutic outcomes. The downstream consequences of the changes observed in this study merits further investigations

The Patterns of High-Level Magnetic Activity Occurring on the Surface of V1285 Aql: The OPEA Model of Flares and DFT Models of Stellar Spots

Statistically analyzing Johnson UBVR observations of V1285 Aql during the three observing seasons, both activity level and behavior of the star are discussed in respect to obtained results. We also discuss the out-of-flare variation due to rotational modulation. Eighty-three flares were detected in the U-band observations of season 2006 . First, depending on statistical analyses using the independent samples t-test, the flares were divided into two classes as the fast and the slow flares. According to the results of the test, there is a difference of about 73 s between the flare-equivalent durations of slow and fast flares. The difference should be the difference mentioned in the theoretical models. Second, using the one-phase exponential association function, the distribution of the flare-equivalent durations versus the flare total durations was modeled. Analyzing the model, some parameters such as plateau, half-life values, mean average of the flare-equivalent durations, maximum flare rise, and total duration times are derived. The plateau value, which is an indicator of the saturation level of white-light flares, was derived as 2.421{\pm}0.058 s in this model, while half-life is computed as 201 s. Analyses showed that observed maximum value of flare total duration is 4641 s, while observed maximum flare rise time is 1817 s. According to these results, although computed energies of the flares occurring on the surface of V1285 Aql are generally lower than those of other stars, the length of its flaring loop can be higher than those of more active stars.Comment: 44 pages, 10 figures, 5 tables, 2011PASP..123..659

Analysis and modeling of high temporal resolution spectroscopic observations of flares on AD Leo

We report the results of a high temporal resolution spectroscopic monitoring of the flare star AD Leo. During 4 nights, more than 600 spectra were taken in the optical range using the Isaac Newton Telescope (INT) and the Intermediate Dispersion Spectrograph (IDS). We have observed a large number of short and weak flares occurring very frequently (flare activity > 0.71 hours-1). This is in favour of the very important role that flares can play in stellar coronal heating. The detected flares are non white-light flares and, though most of solar flares belong to this kind, very few such events had been previously observed on stars. The behaviour of different chromospheric lines (Balmer series from H_alpha to H_11, Ca II H & K, Na I D_1 & D_2, He I 4026 AA and He I D_3) has been studied in detail for a total of 14 flares. We have also estimated the physical parameters of the flaring plasma by using a procedure which assumes a simplified slab model of flares. All the obtained physical parameters are consistent with previously derived values for stellar flares, and the areas - less than 2.3% of the stellar surface - are comparable with the size inferred for other solar and stellar flares. Finally, we have studied the relationships between the physical parameters and the area, duration, maximum flux and energy released during the detected flares.Comment: Latex file with 17 pages, 11 figures. Available at http://www.ucm.es/info/Astrof/invest/actividad/actividad_pub.html Accepted for publication in: Astronomy & Astrophysics (A&A

Photometric Variability in the Ultracool Dwarf BRI 0021-0214: Possible Evidence for Dust Clouds

We report CCD photometric monitoring of the nonemission ultracool dwarf BRI 0021-0214 (M9.5) obtained during 10 nights in 1995 November and 4 nights in 1996 August, with CCD cameras at 1 m class telescopes on the observatories of the Canary Islands. We present differential photometry of BRI 0021-0214, and we report significant variability in the I-band light curve obtained in 1995. A periodogram analysis finds a strong peak at a period of 0.84 day. This modulation appears to be transient because it is present in the 1995 data but not in the 1996 data. We also find a possible period of 0.20 day, which appears to be present in both the 1995 and 1996 datasets. However, we do not find any periodicity close to the rotation period expected from the spectroscopic rotational broadening (< 0.14 day). BRI 0021-0214 is a very inactive object, with extremely low levels of Halpha and X-ray emission. Thus, it is unlikely that magnetically induced cool spots can account for the photometric variability. The photometric variability of BRI 0021-0214 could be explained by the presence of an active meteorology that leads to inhomogeneous clouds on the surface. The lack of photometric modulation at the expected rotational period suggests that the pattern of surface features may be more complicated than previously anticipated.Comment: Accepted for publication in ApJ. 26 pages, 13 figures include

Proton tracking in a high-granularity Digital Tracking Calorimeter for proton CT purposes

Radiation therapy with protons as of today utilizes information from x-ray CT in order to estimate the proton stopping power of the traversed tissue in a patient. The conversion from x-ray attenuation to proton stopping power in tissue introduces range uncertainties of the order of 2-3% of the range, uncertainties that are contributing to an increase of the necessary planning margins added to the target volume in a patient. Imaging methods and modalities, such as Dual Energy CT and proton CT, have come into consideration in the pursuit of obtaining an as good as possible estimate of the proton stopping power. In this study, a Digital Tracking Calorimeter is benchmarked for proof-of-concept for proton CT purposes. The Digital Tracking Calorimeteris applied for reconstruction of the tracks and energies of individual high energy protons. The presented prototype forms the basis for a proton CT system using a single technology for tracking and calorimetry. This advantage simplifies the setup and reduces the cost of a proton CT system assembly, and it is a unique feature of the Digital Tracking Calorimeter. Data from the AGORFIRM beamline at KVI-CART in Groningen in the Netherlands and Monte Carlo simulation results are used to in order to develop a tracking algorithm for the estimation of the residual ranges of a high number of concurrent proton tracks. The range of the individual protons can at present be estimated with a resolution of 4%. The readout system for this prototype is able to handle an effective proton frequency of 1 MHz by using 500 concurrent proton tracks in each readout frame, which is at the high end range of present similar prototypes. A future further optimized prototype will enable a high-speed and more accurate determination of the ranges of individual protons in a therapeutic beam.Comment: 21 pages, 8 figure

The Chromospheric Activity and Ages of M Dwarf Stars in Wide Binary Systems

We investigate the relationship between age and chromospheric activity for 139 M dwarf stars in wide binary systems with white dwarf companions. The age of each system is determined from the cooling age of its white dwarf component. The current limit for activity-age relations found for M dwarfs in open clusters is 4 Gyr. Our unique approach to finding ages for M stars allows for the exploration of this relationship at ages older than 4 Gyr. The general trend of stars remaining active for a longer time at later spectral type is confirmed. However, our larger sample and greater age range reveals additional complexity in assigning age based on activity alone. We find that M dwarfs in wide binaries older than 4 Gyr depart from the log-linear relation for clusters and are found to have activity at magnitudes, colors and masses which are brighter, bluer and more massive than predicted by the cluster relation. In addition to our activity-age results, we present the measured radial velocities and complete space motions for 161 white dwarf stars in wide binaries.Comment: 22 pages including 9 figures and 5 tables. Accepted for publication in The Astronomical Journa

A detailed study of the rise phase of a long duration X-ray flare in the young star TWA 11B

We analyzed a long duration flare observed in a serendipitous XMM-Newton detection of the M star CD-39 7717B (TWA 11B), member of the young stellar association TW Hya (~ 8 Myr). Only the rise phase (with a duration of ~ 35 ks) and possibly the flare peak were observed. We took advantage of the high count-rate of the X-ray source to carry out a detailed analysis of its spectrum during the whole exposure. After a careful analysis, we interpreted the rise phase as resulting from the ignition of a first group of loops (event A) which triggered a subsequent two-ribbon flare (event B). Event A was analyzed using a single-loop model, while a two-ribbon model was applied for event B. Loop semi-lengths of ~ 4 R* were obtained. Such large structures had been previously observed in very young stellar objects (~ 1 - 4 Myr). This is the first time that they have been inferred in a slightly more evolved star. The fluorescent iron emission line at 6.4 keV was detected during event B. Since TWA 11B seems to have no disk, the most plausible explanation found for its presence in the X-ray spectrum of this star is collisional - or photo- ionization. As far as we are concerned, this is only the third clear detection of Fe photospheric fluorescence in stars other than the Sun.Comment: Accepted for publication in ApJ. 15 pages, 9 figure

Low-Mass Dwarf Template Spectra from the Sloan Digital Sky Survey

We present template spectra of low-mass (M0-L0) dwarfs derived from over 4,000 Sloan Digital Sky Survey (SDSS) spectra. These composite spectra are suitable for use as medium-resolution (R ~ 1,800) radial velocity standards. We report mean spectral properties (molecular bandhead strengths,equivalent widths) and use the templates to investigate the effects of magnetic activity and metallicity on the spectroscopic and photometric properties of low-mass stars.Comment: 16 pages, 11 Figures, accepted to the Astronomical Journal, low-resolution figures included. Full-resolution images can be found at http://www.astro.washington.edu/bochansk/lowmass.pd

Anomaly in the stability limit of liquid helium 3

We propose that the liquid-gas spinodal line of helium 3 reaches a minimum at 0.4 K. This feature is supported by our cavitation measurements. We also show that it is consistent with extrapolations of sound velocity measurements. Speedy [J. Phys. Chem. 86, 3002 (1982)] previously proposed this peculiar behavior for the spinodal of water and related it to a change in sign of the expansion coefficient alpha, i. e. a line of density maxima. Helium 3 exhibits such a line at positive pressure. We consider its extrapolation to negative pressure. Our discussion raises fundamental questions about the sign of alpha in a Fermi liquid along its spinodal.Comment: 5 pages, 3 figure

New periodic variable stars coincident with ROSAT sources discovered using SuperWASP

We present optical lightcurves of 428 periodic variable stars coincident with ROSAT X-ray sources, detected using the first run of the SuperWASP photometric survey. Only 68 of these were previously recognised as periodic variables. A further 30 of these objects are previously known pre-main sequence stars, for which we detect a modulation period for the first time. Amongst the newly identified periodic variables, many appear to be close eclipsing binaries, their X-ray emission is presumably the result of RS CVn type behaviour. Others are probably BY Dra stars, pre-main sequence stars and other rapid rotators displaying enhanced coronal activity. A number of previously catalogued pulsating variables (RR Lyr stars and Cepheids) coincident with X-ray sources are also seen, but we show hat these are likely to be misclassifications. We identify four objects which are probable low mass eclipsing binary stars, based on their very red colour and light curve morphology