10 research outputs found
Comment to the paper : Collapse of the vortex-lattice inductance and shear modulus at the melting transition in untwinned YBaCuO, by Matl \QTR{em}{et al.}
In a recent paper, Matl et al present a high-frequency study of the complex
resistivity of a pinned vortex lattice in YBaCuO . They focus on the
inductive-to-resistive transition which is investigated as a function of
temperature at a constant field T, so that the transition is associated
with the vanishing of vortex pinning strength. To our view, their conclusions
rely on a rather brittle experimental body and the collapse of C66 results from
an involved analysis of the finite frequency corrections to .
These corrections are not necessary since the complex frequency spectrum has
been previously interpreted by the two modes model, first proposed for low Tc
materials. We think that it is more adequate to interpret the present data and
should be at least considered.Comment: 4pages tex. submitted to PR
Collapse of the vortex-lattice inductance and shear modulus at the melting transition in untwinned
The complex resistivity of the vortex lattice in an
untwinned crystal of 93-K has been measured at frequencies
from 100 kHz to 20 MHz in a 2-Tesla field ,
using a 4-probe RF transmission technique that enables continuous measurements
versus and temperature . As is increased, the inductance increases steeply to a cusp
at the melting temperature , and then undergoes a steep collapse
consistent with vanishing of the shear modulus . We discuss in detail
the separation of the vortex-lattice inductance from the `volume' inductance,
and other skin-depth effects. To analyze the spectra, we consider a weakly
disordered lattice with a low pin density. Close fits are obtained to
over 2 decades in . Values of the pinning parameter
and shear modulus obtained show that collapses by
over 4 decades at , whereas remains finite.Comment: 11 pages, 8 figures, Phys. Rev. B, in pres
Head-to-head comparison of direct-input RT-PCR and RT-LAMP against RT-qPCR on extracted RNA for rapid SARS-CoV-2 diagnostics
SUMMARYViral pandemics, such as Covid-19, pose serious threats to human societies. To control the spread of highly contagious viruses such as SARS-CoV-2, effective test-trace-isolate strategies require population-wide, systematic testing. Currently, RT-qPCR on extracted RNA is the only broadly accepted test for SARS-CoV-2 diagnostics, which bears the risk of supply chain bottlenecks, often exaggerated by dependencies on proprietary reagents. Here, we directly compare the performance of gold standard diagnostic RT-qPCR on extracted RNA to direct input RT-PCR, RT-LAMP and bead-LAMP on 384 primary patient samples collected from individuals with suspected Covid-19 infection. With a simple five minute crude sample inactivation step and one hour of total reaction time, we achieve assay sensitivities of 98% (direct RT-PCR), 93% (bead-LAMP) and 82% (RT-LAMP) for clinically relevant samples (diagnostic RT-qPCR Ct <35) and a specificity of >98%. For direct RT-PCR, our data further demonstrate a perfect agreement between real-time and end-point measurements, which allow a simple binary classification similar to the powerful visual readout of colorimetric LAMP assays. Our study provides highly sensitive and specific, easy to implement, rapid and cost-effective alternatives to diagnostic RT-qPCR tests.</jats:p
Induction Therapy With ATG Compared With Anti-IL2 Basiliximab in Low–Immunologic Risk Kidney Transplant Recipients
Effects of Low-Intensity Electromagnetic Fields on the Proliferation and Differentiation of Cultured Mouse Bone Marrow Stromal Cells
Steroid Avoidance or Withdrawal After Renal Transplantation Increases the Risk of Acute Rejection but Decreases Cardiovascular Risk. A Meta-Analysis
Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq
AbstractThe COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands of samples in parallel. SARSeq relies on next generation sequencing of multiple amplicons generated in a multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous and scalable assignment of reads to individual samples. We calibrate SARSeq on SARS-CoV-2 synthetic RNA, virions, and hundreds of human samples of various types. Robustness and sensitivity were virtually identical to quantitative RT-PCR. Double-blinded benchmarking to gold standard quantitative-RT-PCR performed by human diagnostics laboratories confirms this high sensitivity. SARSeq can be used to detect Influenza A and B viruses and human rhinovirus in parallel, and can be expanded for detection of other pathogens. Thus, SARSeq is ideally suited for differential diagnostic of infections during a pandemic.</jats:p
