Classification of the relative positions between a small ellipsoid and an elliptic paraboloid

©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/. This version of the article Brozos-Vázquez, M., Pereira-Sáez, M. J., Souto-Salorio, M. J., & Tarrío-Tobar, A. D. (2019). “Classification of the relative positions between a small ellipsoid and an elliptic paraboloid” has been accepted for publication in Computer Aided Geometric Design, 72, 34–48. The Version of Record is available online at https://doi.org/10.1016/j.cagd.2019.05.002.[Abstract]: We classify all the relative positions between an ellipsoid and an elliptic paraboloid when the ellipsoid is small in comparison with the paraboloid (small meaning that the two surfaces cannot be tangent at two points simultaneously when one is moved with respect to the other). This provides an easy way to detect contact between the two surfaces by a direct analysis of the coefficients of a fourth degree polynomial.The authors wish to thank the referees for extremely valuable comments and suggestions, which were essential to improve the final version of the paper. Supported by Projects ED431F 2017/03, TIN2017-85160-C2-1-R, MTM2016-75897-P and MTM2016-78647-P (AEI/FEDER, UE).Xunta de Galicia; ED431F 2017/0

The Nup84 complex coordinates the DNA damage response to warrant genome integrity

DNA lesions interfere with cellular processes such as transcription and replication and need to be adequately resolved to warrant genome integrity. Beyond their primary role in molecule transport, nuclear pore complexes (NPCs) function in other processes such as transcription, nuclear organization and DNA double strand break (DSB) repair. Here we found that the removal of UV-induced DNA lesions by nucleotide excision repair (NER) is compromised in the absence of the Nup84 nuclear pore component. Importantly, nup84Δ cells show an exacerbated sensitivity to UV in early S phase and delayed replication fork progression, suggesting that unrepaired spontaneous DNA lesions persist during S phase. In addition, nup84Δ cells are defective in the repair of replication-born DSBs by sister chromatid recombination (SCR) and rely on post-replicative repair functions for normal proliferation, indicating dysfunctions in the cellular pathways that enable replication on damaged DNA templates. Altogether, our data reveal a central role of the NPC in the DNA damage response to facilitate replication progression through damaged DNA templates by promoting efficient NER and SCR and preventing chromosomal rearrangements.European Research Council ERC2014 AdG669898 TARLOOPMinisterio de Economía y Competitividad BFU2016-75058-PJunta de Andalucía PA12-BIO123

Systematic review of population-based bladder cancer registries : How criteria heterogeneity affects the comparison of incidences

The population-based registry of bladder cancer (BC) raises specific problems intrinsic to the tumor, as the inclusion of noninfiltrating, potentially malignant and multiple tumors. We performed a systematic review (PRISMA guidelines) of population-based BC registries to obtain information on their geographic areas involved, last dates of real incidence of BC, and rules coding used in BC for uncertain behavior, in situ and multiple tumors. Using MEDLINE and Google Scholar, we identified scientific publications of in the last 10 years in English or Spanish, whether they were related to a national or international cancer registry, provided information on registry rules, and provided data on the incidence of BC. After the first screening, a total of 194 references were obtained. After a second analysis, three registries were selected: International Agency for Research on Cancer (IARC) is a world registry providing real incidence of BC in the period 2008-2012. Surveillance, Epidemiology, and End Results (SEER) Program registered incidence until 2017 in more than 90% of the US population. Spanish Network of Cancer Registries (REDECAN) unifies 14 Spanish registries (27.4% of the population) with real incidence data from 2010 to 2015. The coding and inclusion rules have been modified, but currently, most registries include BC in situ and uncertain behavior tumors. Whenever a new case occurs 36 months after a previous diagnosis, SEER registers those as multiple incident cancers in the same location, while IARC and REDECAN only allow one cancer per location during the lifespan of the patient. Comparison of the incidence of BC among different population-based cancer registries is prone to bias due to the methodological differences regarding the inclusion of carcinomas in situ, indeterminate, and multiple tumors. A good cancer registry could provide better surveillance strategies for BC patients. The bladder cancer registry is complex due to the peculiarities of urothelial tumors. The heterogeneity in the rules to define carcinomas in situ, indeterminate and multiple tumors interfere with the comparability of incidence of bladder cancer

Analysis of the traditional methods of education and learning of the collective sports in the primary education and innovative didactic proposals

[Resumen] La metodología empleada en la enseñanza de los deportes colectivos en los centros educativos está desactualizada. El profesorado implicado no valora la evolución que este ámbito sufrió en las últimas décadas y sigue echando mano de los procesos educativos más tradicionales provocando grandes fracasos en el aprendizaje de dichos deportes. Este trabajo realiza una profunda revisión bibliográfica y elabora una unidad didáctica para un aula de sexto de educación primaria. Esta intervención educativa se basa en las técnicas de enseñanza y aprendizaje de los modelos comprensivos, cognitivos, integrados y constructivistas[Abstract] The methodology employed in the education of the collective sports in the educational centres is not updated. The professors involved do not value the evolution that this field suffered in the last decades and follow resorting to the educational processes but traditional causing big failures in the learning of these sports. This work realises a deep bibliographic review and elaborates a didactic unit for a classroom of sixth of primary education. This educational intervention bases in the technicians of education an learning of the understanding models, cognitive, integrated and consturctivis

Results on spatio-temporal estimation of temperature and soil moisture in la Colmena (Paraguay)

This paper presents results on spatio-temporal estimation of agricultural soil variables using a novel methodology, called kinky inference. The methodology is applied to the temperature and soil moisture estimation in a bell pepper (Capsicum annuum) crop in La Colmena, Paraguay. Data is collected with in-situ devices equipped with sensors and endowed with Communication isture estimation in a bell pepper (Capsicum annuum) crop in La Colmena, Paraguay. Data is collected with in-situ devices equipped with sensors and endowed with communication capabilities to send the information to the cloud. The proposed methodology is able to learn from the received data, and produces reliable estimations for any spatial and temporal locations

Synthesis and Characterization of Elongated-Shaped Silver Nanoparticles as a Biocompatible Anisotropic SERS Probe for Intracellular Imaging: Theoretical Modeling and Experimental Verification

Progress in the field of biocompatible SERS nanoparticles has promising prospects for biomedical applications. In this work, we have developed a biocompatible Raman probe by combining anisotropic silver nanoparticles with the dye rhodamine 6G followed by subsequent coating with bovine serum albumin. This nanosystem presents strong SERS capabilities in the near infrared (NIR) with a very high (2.7 × 107) analytical enhancement factor. Theoretical calculations reveal the effects of the electromagnetic and chemical mechanisms in the observed SERS effect for this nanosystem. Finite element method (FEM) calculations showed a considerable near field enhancement in NIR. Using density functional quantum chemical calculations, the chemical enhancement mechanism of rhodamine 6G by interaction with the nanoparticles was probed, allowing us to calculate spectra that closely reproduce the experimental results. The nanosystem was tested in cell culture experiments, showing cell internalization and also proving to be completely biocompatible, as no cell death was observed. Using a NIR laser, SERS signals could be detected even from inside cells, proving the applicability of this nanosystem as a biocompatible SERS probe.España, Regional Ministry of Economy, Junta de Andalucía, P07-FQM-02595 (to CC), P10-FQM-06615 (to JMOM), P10-CTS-6928 (to DP) and PAIDI2020 Program (FQM319 to RFM and CTS677 to DP)Junta de Andalucía, PI-0070/2008 (to PZ) and PI-0068/2008 (to DP

Diseño de un sistema de control de inventario en el restaurante Casa del Café, en la ciudad de Managua en el año 2001

Tesis (Ingeniero Industrial)--Universidad Americana, Managua, 2002La presente tesis diseña un sistema de control de inventario en el restaurante Casa del Café, conociendo y controlando las existencias de cada producto en cada bodega de almacenamiento o área, aumentando la eficiencia en las operaciones y la satisfacción de los clientes, disminuyendo los costos de inventario aplicando las diferentes herramientas ingenieriles adquiridas en el desarrollo de nuestra formación profesional

Exclusive polymerases repairing DNA breaks the same magics from bacteria to man

Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 28-10-201

Modification of betanodavirus virulence by substitutions in the 3’ terminal region of RNA2

Betanodaviruses have bi-segmented positive-sense RNA genomes, consisting of RNAs 1 and 2. For some members of the related genus alphanodavirus, the 3' terminal 50 nucleotides (nt) of RNA2, including a predicted stem-loop structure (3'SL), are essential for replication. We investigate the possible existence and role of a similar structure in a reassortant betanodavirus strain (RGNNV/SJNNV). In this study, we developed three recombinant strains containing nucleotide changes at positions 1408 and 1412. Predictive models showed stem-loop structures involving nt 1398–1421 of the natural reassortant whereas this structure is modified in the recombinant viruses harbouring point mutations r1408 and r1408– 1412, but not in r1412. Results obtained from infectivity assays showed differences between the reference strains and the mutants in both RNA1 and RNA2 synthesis. Moreover, an imbalance between the synthesis of both segments was demonstrated, mainly with the double mutant. All these results suggest an interaction between RNA1 and the 3' non-coding regions (3¢NCR) of RNA2. In addition, the significant attenuation of the virulence for Senegalese sole and the delayed replication of r1408–1412 in brain tissues may point to an interaction of RNA2 with host cellular proteinsThis work was supported by grant AGL2014-54532-C2-2-R from the Ministerio de Innovación y Competitividad (Spain), co-funded by FEDERS