Chemical Composition and Bioactive Antioxidants Obtained by Microwave-Assisted Extraction of Cyperus esculentus L. By-products: A Valorization Approach

Tiger nut is highly appreciated in the Mediterranean basin by the large number of nutritional advantages offered by a beverage, called “horchata,” which is directly obtained from the tuber of Cyperus esculentus L. However, the current tiger nut harvesting and processing practices generate a large number of residues, mainly a solid by-product after processing and the plant that remains spread out in the fields. In this work the plant residues have been fully characterized to get a clear picture of the possibilities for its valorization to generate products with high added value. Several analytical techniques have been applied to obtain data to assess the real possibilities of these residues in advanced applications in the food, packaging and nutrition sectors. Results on the compositional and elemental analysis, monosaccharide composition, phenolic concentration, and antioxidant capacity were obtained from the dry powder (DP). The high content of α-cellulose (47.2 ± 1.8%) in DP could open new possibilities for these residues as raw material in the production of cellulose nanoentities. Many essential minerals with nutritional interest (Na, Mg, Ca, Mn, Fe, Cu, and Zn) and free sugars (xylose, arabinose, glucose, and galacturonic acid) were identified in the DP making it an interesting source of valuable nutrients. The total carbohydrate content was 171 ± 31 mg gdm–1. In addition, microwave-assisted extraction (MAE) was used to obtain extracts rich in polyphenolic compounds. A Box–Behnken design (BBD) was used, and the optimal extraction conditions predicted by the model were 80°C, 18 min, ethanol concentration 40% (v/v), and solvent volume 77 mL, showing an extraction yield of 2.27 ± 0.09%, TPC value was 136 ± 3 mgGAE 100 gdm–1 and antioxidant capacity by the ABTS method was 8.41 ± 0.09 μmoltrolox gdm–1. Other assays (FRAP and DPPH) were also tested, confirming the high antioxidant capacity of DP extracts. Some polyphenols were identified and quantified: p-coumaric (7.67 ± 0.16 mg 100 gdm–1), ferulic (4.07 ± 0.01 mg 100 gdm–1), sinapinic (0.50 ± 0.01 mg 100 gdm–1) and cinnamic acids (1.10 ± 0.03 mg 100 gdm–1), 4-hydroxybenzaldehyde (1.28 ± 0.06 mg 100 gdm–1), luteolin (1.03 ± 0.01 mg 100 gdm–1), and naringenin (0.60 ± 0.01 mg 100 gdm–1). It can be concluded that C. esculentus L. residues obtained from the tiger nut harvesting and horchata processing could be an important source of high value compounds with potential uses in different industrial sectors, while limiting the environmental hazards associated with the current agricultural practices.This study was supported by the Spanish Ministry of Science and Innovation (Refs. PID2020-116496RB-C21 and PDC2021-121345-C21)

Recent Trends in the Analysis of Chemical Contaminants in Beverages

Chemical contaminants should not be present in beverages for human consumption, but could eventually be ingested by consumers as they may appear naturally from the environment or be produced by anthropogenic sources. These contaminants could belong to many different chemical sources, including heavy metals, amines, bisphenols, phthalates, pesticides, perfluorinated compounds, inks, ethyl carbamate, and others. It is well known that these hazardous chemicals in beverages can represent a severe threat by the potential risk of generating diseases to humans if no strict quality control is applied during beverages processing. This review compiles the most updated knowledge of the presence of potential contaminants in various types of beverages (both alcoholic and non-alcoholic), as well as in their containers, to prevent undesired migration. Special attention is given to the extraction and pre-concentration techniques applied to these samples, as well as to the analytical techniques necessary for the determination of chemicals with a potential contaminant effect. Finally, an overview of the current legislation is carried out, as well as future trends of research in this field.Authors would like to thank Spanish Ministry of Science, Innovation and Universities (MAT2017-84909-C2-1-R) and Generalitat Valenciana (IDIFEDER/2018/007) by their support in this work

Emociones en la intención de compra del turista: el vino, la visita a la bodega y una noticia

El enoturismo ha servido a las bodegas para vender vino y desarrollar sus marcas, pero tienen un desconocimiento acerca del grado en el que las emociones producidas por el propio vino y la visita a la bodega, así como de las noticias sobre el vino influyen en la compra de dicho vino por el turista. Mediante la aplicación de ecuaciones estructurales a una muestra de 600 turistas se compara la capacidad explicativa de las emociones producidas por el vino, la visita a la bodega y una noticia sobre vino en la intención de compra de vino. Los resultados demuestran la mayor importancia de las emociones producidas por el vino, seguida de las producidas por la visita y la noticia genera escasa influencia en la intención de compra. Finalmente, se muestran relevantes implicaciones para la gestión y futuras investigaciones.Wine tourism has served the wineries to sell wine and develop their brands, but they have a lack of knowledge about the degree to which the emotions produced by the wine itself and the visit to the winery, as well as the news about wine influence the purchase of such wine by the tourist. Through the application of structural equations to a sample of 600 tourists is compared the explanatory capacity of the emotions produced by the wine, the visit to the winery and a news about wine in the intention to buy wine. The results show the greater importance of the emotions produced by the wine, followed by those by the visit and the news generates lower influence on the intention to buy. Finally, relevant implications for management and future research are shown

Valorization of Agro-Industrial Wastes by Ultrasound-Assisted Extraction as a Source of Proteins, Antioxidants and Cutin: A Cascade Approach

The use of agro-industrial wastes to obtain compounds with a high added-value is increasing in the last few years in accordance with the circular economy concept. In this work, a cascade extraction approach was developed based on ultrasound-assisted extraction (UAE) for tomato, watermelon, and apple peel wastes. The protein and antioxidant compounds were obtained during the first extraction step (NaOH 3 wt.%, 98.6 W, 100% amplitude, 6.48 W/cm2, 6 min). The watermelon peels (WP) showed higher proteins and total phenolic contents (857 ± 1 mg BSA/g extract and 107.2 ± 0.2 mg GAE/100 g dm, respectively), whereas the highest antioxidant activity was obtained for apple peels (1559 ± 20 µmol TE/100 g dm, 1767 ± 5 µmol TE/100 g dm, and 902 ± 16 µmol TE/100 g dm for ABTS, FRAP and DPPH assays, respectively). The remaining residue obtained from the first extraction was subsequently extracted to obtain cutin (ethanol 40 wt.%, 58 W, 100% amplitude, 2 W/cm2, 17 min, 1/80 g/mL, pH 2.5). The morphological studies confirmed the great efficiency of UAE in damaging the vegetal cell walls. WP showed a higher non-hydrolysable cutin content (55 wt.% of the initial cutin). A different monomers’ profile was obtained for the cutin composition by GC-MS, with the cutin from tomato and apple peels being rich in polyhydroxy fatty acids whereas the cutin extracted from WP was mainly based on unsaturated fatty acids. All of the cutin samples showed an initial degradation temperature higher than 200 °C, presenting an excellent thermal stability. The strategy followed in this work has proved to be an effective valorization methodology with a high scaling-up potential for applications in the food, pharmaceutical, nutraceutical, cosmetics and biopolymer sectors.This research was funded by the Bio-Based Industries Joint Undertaking under the European Union Horizon 2020 research program (BBI-H2020), ECOFUNCO project, grant number G.A 837863

Nuevas estrategias de aprendizaje para trabajos fin de grado en Ciencia de los Alimentos

Se ha observado un déficit importante en la formación práctica de los estudiantes que deciden llevar a cabo su Trabajo Fin de Grado en el área de Química de los Alimentos. Se propone el desarrollo de nuevas estrategias para que estos alumnos puedan adquirir una formación básica sobre el trabajo en laboratorio químico durante los primeros días de su dedicación al Trabajo Fin de Grado. Para ello se propone el desarrollo de nuevas herramientas basadas en medios audiovisuales y utilización de los medios de que se dispone en la web 2.0 de la UA, moodle y redes sociales para la preparación de nuevos materiales docentes que permitan el aprendizaje de los alumnos implicados en esta tarea. Estos métodos innovadores son validados por los propios alumnos mediante encuestas, pruebas de laboratorio y seguimiento permanente por parte del profesorado con una interacción constante con los alumnos que han decidido acometer su Trabajo Fin de grado en Química de los Alimentos y que deben acreditar un conocimiento mínimo de las cuestiones básicas de la Química así como en la forma de trabajar con seguridad en los laboratorios

Nuevas estrategias de aprendizaje para trabajos fin de grado en Ciencia de los Alimentos

Se ha observado un déficit importante en la formación práctica de los estudiantes que deciden llevar a cabo su Trabajo Fin de Grado en el área de Química de los Alimentos. Se propone el desarrollo de nuevas estrategias para que estos alumnos puedan adquirir una formación básica sobre el trabajo en laboratorio químico durante los primeros días de su dedicación al Trabajo Fin de Grado. Para ello se propone el desarrollo de nuevas herramientas basadas en medios audiovisuales y utilización de los medios de que se dispone en la web 2.0 de la UA, moodle y redes sociales para la preparación de nuevos materiales docentes que permitan el aprendizaje de los alumnos implicados en esta tarea. Estos métodos innovadores son validados por los propios alumnos mediante encuestas, pruebas de laboratorio y seguimiento permanente por parte del profesorado con una interacción constante con los alumnos que han decidido acometer su Trabajo Fin de grado en Química de los Alimentos y que deben acreditar un conocimiento mínimo de las cuestiones básicas de la Química así como en la forma de trabajar con seguridad en los laboratorios

Antibacterial activity testing methods for hydrophobic patterned surfaces

One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of which ISO 22916 is the standard. To assess the performance of different testing methodologies to analyse the antibacterial activity of hydrophobic surface patterned plastics as part of a Horizon 2020 European research project. Four different testing methods were used to study the antibacterial activity of a patterned film, including the ISO 22916 standard, the immersion method, the touch-transfer inoculation method, and the swab inoculation method, this latter developed specifically for this project. The non-realistic test conditions of the ISO 22916 standard showed this method to be non-appropriate in the study of hydrophobic patterned surfaces. The immersion method also showed no differences between patterned films and smooth controls due to the lack of attachment of testing bacteria on both surfaces. The antibacterial activity of films could be demonstrated by the touch-transfer and the swab inoculation methods, that more precisely mimicked the way of high-touch surfaces contamination, and showed to be the best methodologies to test the antibacterial activity of patterned hydrophobic surfaces. A new ISO standard would be desirable as the reference method to study the antibacterial behaviour of patterned surfaces.The present study was carried out as part of a European project called “Antimicrobial FLEXible POLymers for its use in hospital environments” (FLEXPOL Grant agreement No. 721062) funded by Horizon 2020 Framework Programme for Research and Innovation (2014-2020)

NLRP3 inflammasome activation and symptom burden in KRAS-mutated CMML patients is reverted by IL-1 blocking therapy

Chronic myelomonocytic leukemia (CMML) is frequently associated with mutations in the rat sarcoma gene (RAS), leading to worse prognosis. RAS mutations result in active RAS-GTP proteins, favoring myeloid cell proliferation and survival and inducing the NLRP3 inflammasome together with the apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), which promote caspase-1 activation and interleukin (IL)-1(3 release. Here, we report, in a cohort of CMML patients with mutations in KRAS, a constitutive activation of the NLRP3 inflammasome in monocytes, evidenced by ASC oligomerization and IL-1(3 release, as well as a specific inflammatory cytokine signature. Treatment of a CMML patient with a KRASG12D mutation using the IL-1 receptor blocker anakinra inhibits NLRP3 inflammasome activation, reduces monocyte count, and improves the patient's clinical status, enabling a stem cell transplant. This reveals a basal inflammasome activation in RAS-mutated CMML patients and suggests potential therapeutic applications of NLRP3 and IL-1 blockers

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Valorization of Agro-Industrial Wastes by Ultrasound-Assisted Extraction as a Source of Proteins, Antioxidants and Cutin: A Cascade Approach

The use of agro-industrial wastes to obtain compounds with a high added-value is increasing in the last few years in accordance with the circular economy concept. In this work, a cascade extraction approach was developed based on ultrasound-assisted extraction (UAE) for tomato, watermelon, and apple peel wastes. The protein and antioxidant compounds were obtained during the first extraction step (NaOH 3 wt.%, 98.6 W, 100% amplitude, 6.48 W/cm2, 6 min). The watermelon peels (WP) showed higher proteins and total phenolic contents (857 &plusmn; 1 mg BSA/g extract and 107.2 &plusmn; 0.2 mg GAE/100 g dm, respectively), whereas the highest antioxidant activity was obtained for apple peels (1559 &plusmn; 20 &micro;mol TE/100 g dm, 1767 &plusmn; 5 &micro;mol TE/100 g dm, and 902 &plusmn; 16 &micro;mol TE/100 g dm for ABTS, FRAP and DPPH assays, respectively). The remaining residue obtained from the first extraction was subsequently extracted to obtain cutin (ethanol 40 wt.%, 58 W, 100% amplitude, 2 W/cm2, 17 min, 1/80 g/mL, pH 2.5). The morphological studies confirmed the great efficiency of UAE in damaging the vegetal cell walls. WP showed a higher non-hydrolysable cutin content (55 wt.% of the initial cutin). A different monomers&rsquo; profile was obtained for the cutin composition by GC-MS, with the cutin from tomato and apple peels being rich in polyhydroxy fatty acids whereas the cutin extracted from WP was mainly based on unsaturated fatty acids. All of the cutin samples showed an initial degradation temperature higher than 200 &deg;C, presenting an excellent thermal stability. The strategy followed in this work has proved to be an effective valorization methodology with a high scaling-up potential for applications in the food, pharmaceutical, nutraceutical, cosmetics and biopolymer sectors