Dust polarization in OMC-1

We present ALMA Band 7 polarization observations of the OMC-1 region of the Orion molecular cloud. We find that the polarization pattern observed in the region is likely to have been significantly altered by the radiation field of the >104 L⊙ high-mass protostar Orion Source I. In the protostar’s optically thick disc, polarization is likely to arise from dust self-scattering. In material to the south of Source I – previously identified as a region of ‘anomalous’ polarization emission – we observe a polarization geometry concentric around Source I. We demonstrate that Source I’s extreme luminosity may be sufficient to make the radiative precession time-scale shorter than the Larmor time-scale for moderately large grains (⁠>0.005−0.1μm), causing them to precess around the radiation anisotropy vector (k-RATs) rather than the magnetic field direction (B-RATs). This requires relatively unobscured emission from Source I, supporting the hypothesis that emission in this region arises from the cavity wall of the Source I outflow. This is one of the first times that evidence for k-RAT alignment has been found outside of a protostellar disc or AGB star envelope. Alternatively, the grains may remain aligned by B-RATs and trace gas infall on to the Main Ridge. Elsewhere, we largely find the magnetic field geometry to be radial around the BN/KL explosion centre, consistent with previous observations. However, in the Main Ridge, the magnetic field geometry appears to remain consistent with the larger-scale magnetic field, perhaps indicative of the ability of the dense Ridge to resist disruption by the BN/KL explosion

Mass balance of the Greenland Ice Sheet from 1992 to 2018

In recent decades, the Greenland Ice Sheet has been a major contributor to global sea-level rise1,2, and it is expected to be so in the future3. Although increases in glacier flow4–6 and surface melting7–9 have been driven by oceanic10–12 and atmospheric13,14 warming, the degree and trajectory of today’s imbalance remain uncertain. Here we compare and combine 26 individual satellite measurements of changes in the ice sheet’s volume, flow and gravitational potential to produce a reconciled estimate of its mass balance. Although the ice sheet was close to a state of balance in the 1990s, annual losses have risen since then, peaking at 335 ± 62 billion tonnes per year in 2011. In all, Greenland lost 3,800 ± 339 billion tonnes of ice between 1992 and 2018, causing the mean sea level to rise by 10.6 ± 0.9 millimetres. Using three regional climate models, we show that reduced surface mass balance has driven 1,971 ± 555 billion tonnes (52%) of the ice loss owing to increased meltwater runoff. The remaining 1,827 ± 538 billion tonnes (48%) of ice loss was due to increased glacier discharge, which rose from 41 ± 37 billion tonnes per year in the 1990s to 87 ± 25 billion tonnes per year since then. Between 2013 and 2017, the total rate of ice loss slowed to 217 ± 32 billion tonnes per year, on average, as atmospheric circulation favoured cooler conditions15 and as ocean temperatures fell at the terminus of Jakobshavn Isbræ16. Cumulative ice losses from Greenland as a whole have been close to the IPCC’s predicted rates for their high-end climate warming scenario17, which forecast an additional 50 to 120 millimetres of global sea-level rise by 2100 when compared to their central estimate

OMC-1 dust polarisation in ALMA Band 7: Diagnosing grain alignment mechanisms in the vicinity of Orion Source I

Abstract We present ALMA Band 7 polarisation observations of the OMC-1 region of the Orion molecular cloud. We find that the polarisation pattern observed in the region is likely to have been significantly altered by the radiation field of the >104 L⊙ high-mass protostar Orion Source I. In the protostar’s optically thick disc, polarisation is likely to arise from dust self-scattering. In material to the south of Source I – previously identified as a region of ‘anomalous’ polarisation emission – we observe a polarisation geometry concentric around Source I. We demonstrate that Source I’s extreme luminosity may be sufficient to make the radiative precession timescale shorter than the Larmor timescale for moderately large grains (> 0.005\,-\,0.1\, \mum), causing them to precess around the radiation anisotropy vector (k-RATs) rather than the magnetic field direction (B-RATs). This requires relatively unobscured emission from Source I, supporting the hypothesis that emission in this region arises from the cavity wall of the Source I outflow. This is one of the first times that evidence for k-RAT alignment has been found outside of a protostellar disc or AGB star envelope. Alternatively, the grains may remain aligned by B-RATs and trace gas infall on to the Main Ridge. Elsewhere, we largely find the magnetic field geometry to be radial around the BN/KL explosion centre, consistent with previous observations. However, in the Main Ridge, the magnetic field geometry appears to remain consistent with the larger-scale magnetic field, perhaps indicative of the ability of the dense Ridge to resist disruption by the BN/KL explosion

Location of CD39+ T cell subpopulations within tumors predict differential outcomes in non-small cell lung cancer

Purpose An improved mechanistic understanding of immunosuppressive pathways in non-small cell lung cancer (NSCLC) is important to develop novel diagnostic and therapeutic approaches. Here, we investigate the prognostic significance of the ectonucleotidases CD39 and CD73 in NSCLC.Experimental design The expression and localization of CD39, CD73 and CD103 was digitally quantified in a cohort of 162 early treatment naïve NSCLC patients using multiplex-immunofluorescence and related to patient outcome. Expression among different cell-populations was assessed via flow cytometry. Targeted RNA-Seq was performed on CD4+ and CD8+ T cells from digested NSCLC tumor tissue and single-cell RNA-Seq data was analyzed to investigate the functional significance of CD39+ T cell populations.Results We demonstrate that flow cytometry of early untreated NSCLC patients shows an upregulation of CD39 expression in the tumor tissue among natural killer (NK) cells, fibroblasts and T cells. CD73 expression is mainly found among fibroblasts and Epcam+cells in the tumor tissue. Multiplex Immunofluorescence in a cohort of 162 early untreated NSCLC patients demonstrates that CD39 expression is mainly localized in the tumor stroma while CD73 expression is equally distributed between tumor nest and stroma, and high expression of CD39 and CD73 in the tumor stroma is associated with poor recurrence-free survival (RFS) at 5 years. Additionally, we find that CD8+T cells located in the tumor nest express CD103 and the density of CD39+CD103+CD8+ T cells in the tumor nest predicts improved RFS at 5 years. Targeted RNA-Seq shows that the tumor microenvironment of NSCLC upregulates regulatory pathways in CD4+ T cells and exhaustion in CD8+ T cells, and analysis of a single cell RNA sequencing dataset shows that CD39+CD4+ cells are enriched in Treg signature gene-sets, and CD39+CD103+ cytotoxic T lymphocyte show gene signatures indicative of an exhausted cytotoxic phenotype with upregulated expression of CXCL13.Conclusions Knowledge of patterns of distribution and location are required to understand the prognostic impact of CD39+ T cell populations in NSCLC. This study provides an improved understanding of spatial and functional characteristics of CD39+ T cells and their significance to patient outcome

Location of CD39+ T cell sub-populations within tumours predict differential outcomes in non-small cell lung cancer

Nanostring nCounter targeted RNA Seq. data of CD4+ and CD8+ T cells isolated from human non-cancerous lung tissue and non-small cell lung cancer tumour tissue as shown in Koppensteiner et al. (in submission), "Location of CD39+ T cell sub-populations within tumours predict differential outcomes in non-small cell lung cancer". An improved mechanistic understanding of immunosuppressive pathways in NSCLC is important to develop novel diagnostic and therapeutic approaches. Here, we investigate the prognostic significance of the ectonucleotidases CD39 and CD73 in NSCLC. The expression and localisation of CD39, CD73 and CD103 was digitally quantified in a cohort of 162 early treatment naïve NSCLC patients using multiplex-immunofluorescence and related to patient outcome. Expression amongst different cell-populations was assessed via flow cytometry. Targeted RNA-Seq was performed on CD4+ and CD8+ T cells from digested NSCLC tumour tissue and single-cell RNA-Seq data was analysed to investigate the functional significance of CD39+ T cell populations. We demonstrate that flow cytometry of early untreated NSCLC patients shows an upregulation of CD39 expression in the tumour tissue amongst NK cells, fibroblasts and T cells. CD73 expression is mainly found amongst fibroblasts and Epcam+ cells in the tumour tissue. Multiplex Immunofluorescence in a cohort of 162 early untreated NSCLC patients demonstrates that CD39 expression is mainly localised in the tumour stroma while CD73 expression is equally distributed between tumour nest and stroma, and high expression of CD39 and CD73 in the tumour stroma is associated with poor RFS at 5 years. Additionally, we find that CD8+ T cells located in the tumour nest express CD103 and the density of CD39+CD103+CD8+ T cells in the tumour nest predicts improved RFS at 5 years. Targeted RNA-Seq shows that the TME of NSCLC upregulates regulatory pathways in CD4+ T cells and exhaustion in CD8+ T cells, and analysis of a single cell RNA sequencing dataset shows that CD39+CD4+ cells are enriched in Treg signature gene-sets, and CD39+CD103+ CTL show gene signatures indicative of an exhausted cytotoxic phenotype with upregulated expression of CXCL13. Knowledge of patterns of distribution and location are required to understand the prognostic impact of CD39+ T cell populations in NSCLC. This study provides an improved understanding of spatial and functional characteristics of CD39+ T cells and their significance to patient outcome

The HASHTAG project : the first submillimeter images of the Andromeda galaxy from the ground

Observing nearby galaxies with submillimeter telescopes on the ground has two major challenges. First, the brightness is significantly reduced at long submillimeter wavelengths compared to the brightness at the peak of the dust emission. Second, it is necessary to use a high-pass spatial filter to remove atmospheric noise on large angular scales, which has the unwelcome side effect of also removing the galaxy's large-scale structure. We have developed a technique for producing high-resolution submillimeter images of galaxies of large angular size by using the telescope on the ground to determine the small-scale structure (the large Fourier components) and a space telescope (Herschel or Planck) to determine the large-scale structure (the small Fourier components). Using this technique, we are carrying out the HARP and SCUBA-2 High Resolution Terahertz Andromeda Galaxy Survey (HASHTAG), an international Large Program on the James Clerk Maxwell Telescope, with one aim being to produce the first high-fidelity high-resolution submillimeter images of Andromeda. In this paper, we describe the survey, the method we have developed for combining the space-based and ground-based data, and we present the first HASHTAG images of Andromeda at 450 and 850 mu m. We also have created a method to predict the CO(J = 3-2) line flux across M31, which contaminates the 850 mu m band. We find that while normally the contamination is below our sensitivity limit, it can be significant (up to 28%) in a few of the brightest regions of the 10 kpc ring. We therefore also provide images with the predicted line emission removed

Mass balance of the Greenland Ice Sheet from 1992 to 2018

The Greenland Ice Sheet has been a major contributor to global sea-level rise in recent decades1,2, and it is expected to continue to be so3. Although increases in glacier flow4,5,6 and surface melting7,8,9 have been driven by oceanic10,11,12 and atmospheric13,14 warming, the magnitude and trajectory of the ice sheet’s mass imbalance remain uncertain. Here we compare and combine 26 individual satellite measurements of changes in the ice sheet’s volume, flow and gravitational potential to produce a reconciled estimate of its mass balance. The ice sheet was close to a state of balance in the 1990s, but annual losses have risen since then, peaking at 345 ± 66 billion tonnes per year in 2011. In all, Greenland lost 3,902 ± 342 billion tonnes of ice between 1992 and 2018, causing the mean sea level to rise by 10.8 ± 0.9 millimetres. Using three regional climate models, we show that the reduced surface mass balance has driven 1,964 ± 565 billion tonnes (50.3 per cent) of the ice loss owing to increased meltwater runoff. The remaining 1,938 ± 541 billion tonnes (49.7 per cent) of ice loss was due to increased glacier dynamical imbalance, which rose from 46 ± 37 billion tonnes per year in the 1990s to 87 ± 25 billion tonnes per year since then. The total rate of ice loss slowed to 222 ± 30 billion tonnes per year between 2013 and 2017, on average, as atmospheric circulation favoured cooler conditions15 and ocean temperatures fell at the terminus of Jakobshavn Isbræ16. Cumulative ice losses from Greenland as a whole have been close to the rates predicted by the Intergovernmental Panel on Climate Change for their high-end climate warming scenario17, which forecast an additional 70 to 130 millimetres of global sea-level rise by 2100 compared with their central estimate