22 research outputs found
Simultaneous Estimation of Berberine and Quercetin in Pathydi Kada Formulation by Hptlc
- Publication venue
- ASSOC ADVANCEMENT ZOOLOGY , AZADANAGAR COLONY RUSTAMPUR, GORAKHPUR, INDIA, 273001
- Publication date
- 03/11/2023
- Field of study
A sensitive, selective and precise thin-layer chromatographic method has been developed and validated for the analysis of Berberine and Quercetin in Pathydi Kada laboratory prepared and Market formulation. Separation and quantification were achieved by TLC using mobile phase of Ethyl acetate: Methanol: Glacial acetic acid (6: 4: 0.5) v/v/v (Rf 0.2 & 0.84 for Berberine and Quercetin respectively) on precoated silica gel 60F254 aluminum plates and determination was carried out at 254 and 366 nm for berberine and quercetin respectively. The calibration curve was linear in the concentration range of 4-12 μg spot-1. The method was validated for precision, repeatability and accuracy. The proposed method was found to be simple, precise, specific, sensitive and accurate for the quantification of Berberine and Quercetin. This is the first TLC report for the simultaneous estimation of Berberine and Quercetin in Pathydi Kada formulation and may be useful for the routine quality control
Resistive Switching Memory Properties of Electrodeposited Cu2O Thin Films
- Author
- Publication venue
- 'Sumy State University'
- Publication date
- 01/01/2020
- Field of study
The Cu2O thin film was developed using an electrodeposition approach for resistive memory application. The impact of the deposition voltage (1V, 2V, 3V, and 4V) on resistive switching (RS)/memristive properties of Cu2O thin films was studied. The XRD spectrum reveals that deposited Cu2O has a cubic crystal structure. The bipolar RS in Al/Cu2O/FTO device was clearly observed during the current-voltage (I-V) measurement. The basic memristive properties were calculated from I-V data. The charge transport studies suggested that the SCLC mechanism was responsible for device conduction, and RS was due to filamentary effect. The result suggested that the electrodeposition technique is useful to fabricate a memristive device for various applications
Robust estimation of bacterial cell count from optical density
- Author
- Aarnio Tiu
- Aaron Leow Chung Yong
- Abbas Anser
- Abduraimova Aiganym
- Abraham Doris
- Acar Beliz Leyla
- Acosta Joaquin
- Adler Nina
- Adulyanukosol Natthawut
- Agena Ejouan
- Agena Ethan
- Aggarwal Priya
- Aguirre Adriana Lizeth Rubio
- Ahlgren Christopher
- Ahuja Janvi
- Aizik Dror
- Akbary Mina
- Akinfenwa Akinwumi
- Akingbesote Ngozi D.
- Akova Umut
- Aldo Ferdinan
- Alexopoulos Ioannis
- Allen Lucy
- Alonso Alejandro
- Altarawneh Dina
- Alvarado Andres Benjamin Sanchez
- Ambre Sanika
- Ameziane Annissa
- Amheine Khadija
- Amin Ihya Fakhrurizal
- Amir Sayeda Sakina
- Amstel Chiel van
- An Yongyan
- Anand Nithishwer Mouroug
- Ananthakrishnan Sathvik
- Anderson Karl
- Angel Sagi
- Annem Vidhya
- Anson Chung Tsun Ho
- Anwar Mariam
- Appel Alana
- Araya Lucas
- Armstrong Alexander
- Arora Neha
- Arora Vasu
- Arruda Beck
- Ashwin F Amal Jude
- Astapenka Artur
- Astles Ben
- Astorino Gabe
- Aubry Celine
- Avileli Rebecca
- Azad Mahnaz Sabeta
- Babar Rehmat
- Babu Priyanka
- Badalli Turan
- Badash Lior
- Baichman-Kass Amichai
- Baldwin Geoff S.
- Baltensperger Oliver Andreas
- Bao Guanke
- Bao Minyue
- Baronaite Ugne
- Barrat Leon Michael
- Barrington Seth
- Barshap Alon
- Bartzoka Natalia
- Basu Anubhav
- Basulto Cynthia
- Bath Scott
- Battina Rohith
- Bauersachs Daniel
- Beal Jacob
- Beale Renee
- Beard Eleanor
- Beck Selina
- Becker Evan
- Benavides Samantha Ayde Pena
- Bennis Nicole
- Bete Anna
- Bhatt Darshak
- Bhowmick Shilpa
- Bian Weixin
- Bijman Eline Yafele
- Bindt Felix
- Blas Karla Soto
- Bo Tang
- Boada Yadira
- Boer Meine
- Bohner Ariel
- Borovko Irina
- Bott Amie
- Bouwmeester Susan
- Bowman Kyle
- Boxem Mike
- Boxuan Cheng
- Boyce Kira
- Boyer Jess
- Brasas Valentas
- Brewer Mathew
- Briand William
- Brink Matilda
- Browne Emily
- Buckley-Taylor Russell
- Budhan Stephanie
- Buller Lisa
- Burckhardt Nele
- Burgland Zachary
- Burns Frank
- Burridge Matt
- Burud Hanna Nedreberg
- Buson Felipe Xavier
- Butterfield Tom
- Byagathvalli Gaurav
- Byatt Gabriel
- Byun Sumin
- Caballero Amalia
- Cai Fu
- Cai Niangui
- Caldwell Sarah
- Camacho Yazmin
- Camille Monteil
- Campbell Robert
- Cao Qinhao
- Cao Yixin
- Cao Yuze
- Carrillo Leo
- Carter Jonah
- Casas Alexis
- Castillo Nora Esther Torres
- Castillo Sandra
- Cave John
- Chai Ruochen
- Chamiec-Case Elizabeth
- Chan Janice
- Chan Yuan-Yao
- Chandramouli Priyanka
- Chang Annabel
- Chang Catherine
- Chang Kevin
- Chang Thomas
- Chang Ting-Chen
- Chang Yu-Ci
- Chapman Tyler
- Chater Dounia
- Chau Anthony
- Chavez Benjamin
- Chavez Francisco
- Chehade Ibrahim
- Chen Carmen
- Chen Chih-Chiang
- Chen Ching-Wei
- Chen Janice
- Chen Junhong
- Chen Junyang
- Chen Junyu
- Chen Pei-Hong
- Chen Ryan
- Chen Weixuan
- Chen Yan
- Chen Yi-Cian
- Chen Yifei
- Chen Zhuoyang
- Cheng Cheng
- Cheng Haolun
- Cheng Hsuan
- Cheng Yu-Hong
- Cheng Yu-Hsuan
- Chenxi Liang
- Cherupalla Arjun
- Chi Jordi
- Chi Shumei
- Chiang Jason
- Chiang Woody
- Ching Lai Tsz
- Ching Lam Kai
- Ching Law Hiu
- Ching Luk Hau
- Chinnathambi Sathivel
- Cho Sungwoo
- Choi Lee Sze
- Choi Youngeun
- Chou Ryan
- Chou Ryan
- Chu Wai Yan
- Chua Yah Tse Sabrina
- Chuang Yi-Chien
- Chulkova Nadezhda
- Chun Cheung Man
- Chun Emily
- Chun Ng Tsz
- Cifuentes Rodrigo Hernandez
- Clapper Jude
- Clapper Jude
- Clark Brittany
- Clemente Borja Sanchez
- Clerc Valerian
- Clodomir Christina
- Coates Ryan
- Cohen Katherine
- Colant Noelle
- Coleman Sarah
- Colgan Cian
- Cone Eli
- Corre Christophe
- Costa Ben
- Cott Rachel Van
- Craw Darilyn
- Crispim Larissa de Souza
- Crone Michael Andrew
- Crooijmans Marjolein
- Crosby Laurel Constanti
- Crowley Peter
- Cruz Frida
- Cubukcu Hande
- Culfogienis Dylan
- Czekster Clarissa
- Dai Leijie
- Dalangin Rochelin
- Daniel Benjamin
- Daniels Lea
- Das Neves Laura
- Das Diptatanu
- Davis Christina
- De Silva Nimaya
- Deb Shalini S.
- Deepak Kumar S.
- Deibel Emilie
- Dejoie Julianne
- Dejoux Alice
- DeKloe Jackson
- Del Toro Mariano
- Delalez Nicolas
- Delgadillo Sabrina
- Delhomme Jean
- Demchuk Aubrey
- Deng Nian Ruei
- Deng Rui
- Denter Jennifer
- Deshmukh-Reeves Ed
- Despres Philippe C.
- Devi Velvizhi
- Dhaliwal Gurpreet
- Diaz Fernanda
- Ding Shizhi
- Ding Yiming
- Ding Zhipeng
- Dingyue Tang
- Dirven Yvette
- Dissmann Jil-Sophie
- Do Stephanie
- Dogra Arushi
- Dominguez Viana Isabel Perez
- Dong Dongyang
- Dong Haitao
- Dong Jason
- Dong Shan
- Dong Shan
- Dong Shan
- Dong Shangzhi
- Dong Shangzhi
- Doyle David
- Doyle Shannon
- Dube Alexandre
- Dubuc Emilien
- Dunstan Izzy
- Dusseaux Theodore A.
- Dvorkin Steve
- Edelmayer Marco
- Ehrenworth Amy
- Eidloth Franziska
- Eijkens Bruno
- Eilers Keno
- El Moubayed Yorgo
- Elcin Evrim
- Elias Noel
- Elikan Max
- Ellermeier Craig
- Elliott Hannah
- Elsa Yeung Hoi Lam
- Emilov Kristian
- Espinoza Diego
- Evans Rhys
- Faidh Mohammed
- Falgenhauer Elisabeth
- Fan Catherine
- Fang Chengzhu
- Fang Choco
- Fang Xiangyi
- Farhi Einan
- Farias Sebastian
- Farny Natalie
- Farny Natalie G.
- Farrell Jennifer
- Fassler Jan
- Faurdal David
- Faustova Ilona
- Feng Xinhua
- Fernandez Cesar Ibrahym Rodriguez
- Fierro Daniela Olono
- Figueroa Ana Cristina
- Figueroa Jose Arnulfo Juarez
- Fikry Clara
- Filzen Rachael
- Fischer Matthew
- Fitzgerald Kevin
- Fitzgerald Liza
- Flores Vinicius
- Foda Asmaa
- Fong Yuhui Deborah
- Forman Victor
- Frank Stefanie
- Frankel Eva
- Freire Paulo J. C.
- Friedman Yonatan
- Frohlich Lisa
- Fu Wenhan
- Fu Yousi
- Fuentes Ernie
- Fulton Leah
- Fung Ip Ning
- Fung Yam Shing
- Gabrielli Jacopo
- Gahagan Maddison
- Gaitt Tobias
- Gaizauskaite Aukse
- Gallegos Isabella
- Gamoneda Diego
- Gandhi Rishi
- Gao Cecily
- Garavito Paula
- Garcia Chrismar
- Garcia David
- Garrido Delia
- Garulli Elisa
- Gaspar Camila
- Gaudi Alex
- Ge Gao
- Ge Yubin
- George Stephan
- Gerard Antoine
- Gershater Markus
- Ghosh Souvik
- Giannopoulos Alexandros Dimitriou
- Giannoula Yvoni
- Gilman Daniel Barber James
- Glockner Eleanor
- Goenka Anushya
- Goetz Marley
- Gogineni Namrata
- Gokul [NO FIRST NAME IN SCOPUS]
- Gonzalo Gonzalo Saiz
- Goodson Michael
- Gopaul Deshmukh
- Gou Yanchen
- Govers Jolijn
- Graaf Floor de
- Grace Tsui Shing Yan
- Graves Daniel
- Grazulyte Inga
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- Guenther James
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- Guptil Chris
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- Hale Mitch
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- Han Weitang
- Handal Paola
- Hanrong Zhu
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- Hao Junyao
- Hao Mei
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- Haoran Xu
- Haralsvik Anne Kristin
- Hariyani Imtiyaz
- Hartley Zach
- Hassan Sondoss
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- Herrmann Max
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- Ho Tim
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- Hofer Sebastian
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- Holmedal Ingebjorg
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- Huang Fangliang
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- Huang Ray
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- Huang Yansong
- Huang Yaohua
- Huang Yilin
- Huang Yinpin
- Huanying He
- HuaYue Zhang
- Huerta Ana Laura Torres
- Humphreys Christopher
- Hung Chia
- Husnatter Kai
- Huuki Liz
- Huynh Juilanne
- Hwang Michael
- Ibrahim Ahmed
- Ikagawa Yuichiro
- Ikhsan Muhammad
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- Imgold Markus
- Inanc Tugba
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- Isaacs Farren
- Isogai Tatsuki
- Iwuogo Amanda
- Iyer Aditya
- Iyer Tvarita Shivakumar
- Izzati Nurul
- Jacq Annick
- Jain Rishabh
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- Jainarayanan Ashwin Kumar
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- Jasiunas Simas
- Jasti Naveen
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- Jayaraman Srimathi
- Jenakendran Jenaagan
- Jensen Joen Haahr
- Jesse Hayley
- Jha Aryaman
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- Jiacheng Wu
- Jiahe Zhang
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- Kayihan Ceyhun
- Keating Collin
- Kehan Tao
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- Kohabir Kavish
- Kolosova Anastasia
- Kong Cheng Chak
- Kong Linzhen
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- Kostopoulos Ioannis V.
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- Koutelidakis Antonios E.
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- Krabacher Rachel
- Krainer Birgit
- Krauss Elisha
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- Krokos Vasilios
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- Ledalla Karthik
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- leelahakorn Nattawut
- Leeuw Monique de
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- Lekshmi Hema
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- Liu Yuansheng
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- Mao Jiangjiao
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- Preetha R.
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- Ptak Jeffrey
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- Qi Ariel
- Qian Ling
- Qiang Ye
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- Raadam Morten
- Rai Hrishika
- Rajagopal Varshni
- Raju Deeksha
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- Rakhimov Jennifer
- Rambhatla Rahul
- Ramstrom Elin
- Ramzeen Aaron
- Ran Guozheng
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- Romeo Yves
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- Rosa Caio Gomes Tavares
- Rouleau Francois D.
- Ruhnau Johannes
- Ruhnau Johannes
- Run Zhouyan
- Ruscher David
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- Ruzicka Alex
- Ryan Sean
- Ryu Bo Sun
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- Sabandal Angelica
- Sabuwala Burhanuddin
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- Saha Margaret
- Saini Karandip
- Sanchania Vishal
- Sanchez Magaly Aguirre
- Sande Amanda
- Sander Edward
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- Sans Laura
- Santamaria Stefanie
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- Satoh Takehito
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- Sauvageau Dominic
- Saville Luke
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- Sayous Victor
- Schaaf Kaitlin
- Schacht Makayla
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- Schmidtchen Lisbeth
- Schultz Jessica
- Schwarz Dominic
- Scott Allison
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- Zhu Ke
- Zhu Xueqian
- Zi Lihan
- Zilinskaite Nemira
- Zimmermann Andreas
- Zimmermann Jennifer
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- Zukauskaite Kristina
- Zulueta Patricia
- Zvirblyte Justina
- Publication venue
- Publication date
- 01/01/2020
- Field of study
Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data
Calibration of the CMS hadron calorimeters using proton-proton collision data at root s=13 TeV
- Author
- Aarrestad Thea Klaeboe
- Abbaneo Duccio
- Abbiendi Giovanni
- Abbrescia Marcello
- Abdullin Salavat
- Abercrombie Daniel
- Acharya Himal
- Acosta Darin
- Acosta John Gabriel
- Adam Wolfgang
- Adamov George
- Adams Mark Raymond
- Adams Todd
- Adzic Petar
- Afanasiev Serguei
- Agapitos Antonis
- Agarwal Garvita
- Aggleton Robin
- Agram Jean-Laurent
- Aguilar-Benitez Manuel
- Agyel D.
- Ahmad Ashfaq
- Ahmad Muhammad
- Ahmad Muhammad
- Ahuja Sudha
- Akbiyik Melike
- Akchurin Nural
- Akgun Bora
- Albajar Carmen
- Albergo Sebastiano
- Albert Andreas
- Albrow Michael
- Alcaraz Maestre Juan
- Aldaya Martin Maria
- Aleksandrov Aleksandar
- Alexander James
- Alhusseini Mohammad
- Alimena Juliette
- Allen Brandon
- Almond John
- Alvarez Gonzalez Barbara
- Alverson George
- Alves Fábio Lúcio
- Alves Gilvan
- Aly Reham
- Alyari Maral
- Amapane Nicola
- Ambrogi Federico
- Amendola Chiara
- Amin Nick
- Amsler Claude
- Anagnostou Georgios
- Anagul S.
- Anderson Dustin
- Andrea Jeremy
- Andreev Vladimir
- Andreev Yuri
- Andrews Michael Benjamin
- Androsov Konstantin
- Antchev Georgy
- Antunovic Zeljko
- Apanasevich Leonard
- Apollinari Giorgio
- Apresyan Artur
- Apyan Aram
- Araujo Mariana
- Arcaro Daniel
- Arcidiacono Roberta
- Arenton Michael Wayne
- Argiro Stefano
- Arneodo Michele
- Asavapibhop Burin
- Asawatangtrakuldee Chayanit
- Asenov Patrick
- Askew Andrew
- Asmuss Paul
- Assran Yasser
- Atakisi Ismail Okan
- Ather Mohsan Waseem
- Attikis Alexandros
- Auffray Etiennette
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- Autermann Christian
- Auzinger Georg
- Avati Valentina
- Avery Paul
- Avila Carlos
- Ayala Edy
- Azarkin Maksim
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- Yusli Mohd Nizam
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- Zaganidis Nicolas
- Zahid Sema
- Zaleski Shawn
- Zalewski Piotr
- Zanetti Anna
- Zanetti Marco
- Zarubin Anatoli
- Zarucki Mateusz
- Zecchinelli Angelo Giacomo
- Zenaiev Oleksandr
- Zenz Seth Conrad
- Zeuner Wolfram Dietrich
- Zevi Della Porta Giovanni
- Zghiche Amina
- Zhang Aobo
- Zhang Fengwangdong
- Zhang Huaqiao
- Zhang Jingyu
- Zhang Sijing
- Zhang Wenyu
- Zhang Yi
- Zhang Zhicai
- Zhao Jingzhou
- Zhokin Alexander
- Zhu De Hua
- Zhu Ren-Yuan
- Zientek Margaret
- Zlebcik Radek
- Zobec Joze
- Zoi Irene
- Zolkapli Zukhaimira
- Zorbakir Ibrahim Soner
- Zorbilmez Caglar
- Zotto Pierluigi
- Zou David
- Zucchetta Alberto
- Zumerle Gianni
- Zuo Xunwu
- Zuolo Davide
- Álvarez Fernández Adrian
- Özçelik Özlem
- Publication venue
- Publication date
- 01/01/2019
- Field of study
Methods are presented for calibrating the hadron calorimeter system of theCMSetector at the LHC. The hadron calorimeters of the CMS experiment are sampling calorimeters of brass and scintillator, and are in the form of one central detector and two endcaps. These calorimeters cover pseudorapidities vertical bar eta vertical bar ee data. The energy scale of the outer calorimeters has been determined with test beam data and is confirmed through data with high transverse momentum jets. In this paper, we present the details of the calibration methods and accuracy.Peer reviewe
31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two
- Author
- Abassi Yama
- Abelin Jennifer G
- Abolhalaj Milad
- Abraham Tara S
- Adam Ammar
- Adams David
- Adams Homer
- Adams Sarah F
- Adamus Tomasz
- Addepalli Murali
- Addepalli Murali
- Adjei Alex A
- Agapova Larissa
- Agarwala Sanjiv
- Ager Casey
- Aggarwal Charu
- Agnello Giulia
- Agudo Judith
- Aguilar Ethan G
- Aguilera Todd
- Ahamadi Malidi
- Ahn Yong-Oon
- Ahrens Eric T
- Aizer Ayal
- Ajina Reham
- Akerley Wallace
- Al-Muftah Mariam
- Albershardt Tina C
- Albershardt Tina C
- Albrekt Ann-Sofie
- Alekar Shilpa
- Alemany Ramon
- Alemany Ramon
- Alexander Brian
- Allbritton Omaira
- Allen Clint T
- Alley Evan W
- Allison James
- Allison James
- Allison James
- Alston Tesha
- Alt Jürgen
- Alters Susan
- Amatulli Michael
- Anand Snjezana
- Anand Snjezana
- Anand Snjezana
- Anderson Clark
- Anderson Mark
- Andersson Bengt
- Andre Pascale
- Andtbacka Robert H I
- Andtbacka Robert H I
- Angiuoli Sam
- Ansari Tameem
- Anthony Scott
- Antonarakis Emmanuel S
- Antonia Scott J
- Anwar Firoz
- Aquino-Michaels Keston
- Archer Jacob F
- Arina Ainhoa
- Armand Philippe
- Armenta Paul
- Armet Caroline M
- Arnoux Thomas
- Ascarateil Stephane
- Askmyr David
- Atkins Michael
- Atkins Michael B
- Atkinson Victoria
- Au Katrina
- Autio Karen A
- Awad Mark
- Bagarazzi Mark
- Bai Dina
- Baia Gilson
- Baird Jason
- Bajaj Anshika
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- Balboni Tracy
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- Bao Yangyi
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- Barberi Theresa
- Barker Christopher A
- Barnea Eytan
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- Bartkowiak Todd
- Bartlett David
- Bartlett David
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- Bauman Julie
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- Coukos George
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- Dorsey Frank C
- Dovedi Simon
- Downs-Canner Stephanie
- Drake Charles
- Drake Charles G
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- Facciponte John
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- Shamalov Katerina
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- Stefano Pierini
- Steggerda Susanne
- Stein Paul L
- Stelow Edward B
- Sternby Malin
- SternJohn Julius
- Stewart Al
- Stock Wendy
- Stone Geoffrey W
- Stosnach Claudia
- Straub Tobias
- Straus Jane
- Streiner Nicole
- Ståhle Magnus
- Su Yu-Lin
- Sudarshan Naremaddepalli
- Sugarbaker David
- Sukari Ammar
- Sultan Hussein
- Sun Shaoli
- Sundararaman Srividya
- Sung Leonard
- Suresh Rahul
- Surguladze David
- Suryawanshi Satyendra
- Suryawanshi Satyendra
- Suzuki Akiko
- Suzuki Ryuji
- Sweis Randy F
- Taback Bret
- Tagliaferri Mary
- Talbert Erin
- Tallarico John
- Tan Joanne B
- Tan Joanne B L
- Tan Wei
- Tan Yann C
- Tanaka Maki
- Tang Chad
- Tannir Nizar M
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- Taylor Allison
- Taylor Matthew
- Tcherepanova Irina
- ter Meulen Jan
- ter Meulen Jan
- Terabe Masaki
- Terabe Masaki
- Termini James
- Tetzlaff Michael
- Tewary Poonam
- Thakurta Anjan
- Thayer Matt
- Theodoraki Marie-Nicole
- Thimme Robert
- Thomas Portia
- Thomas Remy
- Thomas Vincent
- Thompson John A
- Thounaojam Menaka
- Thounaojam Menaka
- Thurston Gavin
- Tighe Rober
- Tilahun Ashenafi
- Tillé Laure
- Timmerman John
- Tipton Kimberly A
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- Tognon Christina E
- Tomai Mark A
- Toms Anne
- Torno Sebold
- Torrey Heather
- Torti Vince
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- Trivedi Sumita
- Truesdell Peter
- Tsang Kwong
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- Tsaparikos Konstantinos
- Tsouko Efrosini
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- Tuck David
- Tumang Joseph
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- Tyner Jeffery W
- Tähtinen Siri
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- Uecker Darrin
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- Ugel Stefano
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- Uhlik Mark
- Ujhelyi Mihaly
- Ullenhag Gustav
- Unfricht Darryn
- Ungerleider Richard
- Urban Julie
- Urban Julie
- Valsesia-Wittmann Sandrine
- Valsesia-Wittmann Sandrine
- Van der Meijs Pien
- van Vugt Marianne
- van Zutphen Mariëlle
- Vanamee Eva
- Vanderslice Peter
- VanderVeen Laurie
- Vanpouille-Box Claire I
- VanVlasselaer Peter
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- Vetto John
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- Vignali Dario
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- Vogel Katharina
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- Welsh James
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- Wilson Nicholas
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- Wittig Burghardt
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- Wolchok Jedd D
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- Xi Biao
- Xia Leiming
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- Xiang Bo
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- Xiao Hong
- Xiao Zhilan
- Xu Chun-wei
- Xu Chunxiao
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- Xu Xin
- Xu Ya-Ming
- Xu Yitong
- Yadav Mahesh
- Yagiz Kader
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- Yan Jian
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- Yang Ning
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- Yao Shiyin
- Ye Yun-bin
- Yin Catherine
- Ylösmäki Erkko
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- Young Gregory
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- Yu Naichen
- Zafar Sadia
- Zalevsky Jonathan
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- Zalevsky Jonathan
- Zappasodi Roberta
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- Zeh Herbert
- Zelenetz Andrew D
- Zeng Weiping
- Zeng Xue
- Zha Yuanyuan
- Zhang Danhui
- Zhang Jing
- Zhang Ping
- Zhang Qifang
- Zhang Shannon S
- Zhang Theresa
- Zhang Winter
- Zhang Yanping
- Zhao Fei
- Zhao Leyna
- Zhao Xingli
- Zheng Lianxing
- Zheng Wenxin
- Zheng Xianxian
- Zheng Yi
- Zhong Hong
- Zhou Li
- Zhou Xiangjun
- Zippelius Alfred
- Zloza Andrew
- Zloza Andrew
- Zloza Andrew
- Zloza Andrew
- Zloza Andrew
- Zou Jun
- Zuba-Surma Ewa
- Zubkova Olga
- Zureikat Amer
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2016
- Field of study
Background
The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd.
Methods
We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background.
Results
First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001).
Conclusions
In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival
Optimization of surface roughness in milling of EN 24 steel with WC-Coated inserts using response surface methodology: analysis using surface integrity microstructural characterizations
- Author
- Publication venue
- Frontiers Media S.A.
- Publication date
- 01/03/2024
- Field of study
Introduction: Among alloys of medium-carbon and high-strength steel, EN 24 steel is characterised by its nickel-chromium-molybdenum composition. EN 24 steel is highly suitable for application in heavy-duty projects due to its notable resilience to damage, especially when exposed to low temperatures. With the objective of minimising surface irregularities, this research endeavours to enhance the milling process of EN 24 steel by employing coated tungsten carbide (WC) tool inserts.Methods: Feed rate, cutting speed, depth of cut, and cutting fluid are all crucial process factors in the experimental investigation. Four distinct levels are applied to each factor. The research utilises the Design of Experiments (DOE)-based Central Composite Design of Response Surface Methodology. To predict output parameters, mathematical models are developed utilising analysis of variance (ANOVA) for optimisation purposes.Results and discussions: Through the utilisation of multi-objective optimisation, the optimal combination for tungsten carbide inserts was determined, which provided surface irregularities of 0.301 µm. Cutting speed (CS) of 149.507 m/min, feed rate (FR) of 340.27 mm/min, depth of cut (DOC) of 0.599 mm, and cutting fluid (CF) of 12.50 L/min are the optimal parameters. The surface morphologies of the machined workpiece at particular parameter values can be discerned through scanning electron microscope (SEM) analysis, yielding significant insights. The optimal parameters that have been identified provide practical recommendations for improving the milling method of EN 24 steel when tungsten carbide inserts are utilised. Understanding the milling process in its entirety is facilitated by SEM analysis of surface morphologies and microstructures under particular cutting conditions. The morphology and surface irregularities of the machined workpiece are evaluated using profilometry, which provides additional insight into surface integrity. The discourse investigates the potential applications and implications of the results, as well as suggests directions for further study concerning the enhancement of milling processes for similar steel alloys
Spike-time dependent plasticity of tailored ZnO nanorod-based resistive memory for synaptic learning
- Author
- Publication venue
- Elsevier
- Publication date
- 01/12/2023
- Field of study
Metal oxide resistive memory is a potential device that can substantially influence the current roadmap for nonvolatile memory and neuromorphic computing. However, common amorphous oxide-based resistive random-access memory suffers from high forming voltages that complicate circuit design and abrupt SET behavior incompatible with analog weight updates. To overcome such limitations, wurtzite ZnO nanorods were synthesized on a fluorine-doped tin oxide (FTO) substrate and a bipolar resistive memory with the Ag/w-ZnO/FTO stacking sequence was fabricated. The hexagonal NR morphology of w-ZnO with controlled vertical growth and nanochannel formation between the NRs were produced by in situ crystalline growth. This morphology enabled a forming-free switching and an analog switching effect that emulated neuromorphic functionalities such as potentiation–depression and complex spike-time dependent plasticity-based Hebbian learning rules. Importantly, the device exhibited nonabrupt switching behavior suitable for analog weight updates in neuromorphic computing in contrast to conventional resistive memory