Datasets for transcriptomics, q-proteomics and phenotype microarrays of polyphosphate metabolism mutants from Escherichia coli

Indexación: Scopus.Author acknowledges Fondecyt Grants 1120209, 1121170 and Anillo ACT-1107Here, we provide the dataset associated with our research article on the polyphosphate metabolism entitled, “Multi-level evaluation of Escherichia coli polyphosphate related mutants using global transcriptomic, proteomic and phenomic analyses”. By integrating different omics levels (transcriptome, proteome and phenome), we were able to study Escherichia coli polyphosphate mutant strains (Δppk1, Δppx, and Δppk1-ppx). We have compiled here all datasets from DNA microarrys, q-proteomic (Isotope-Coded Protein Labeling, ICPL) and phenomic (Phenotype microarray) raw data we have obtained in all polyP metabolism mutants.http://www.sciencedirect.com/science/article/pii/S2352340917300860?via%3Dihu

El envejecimiento de las pastas de cemento reforzadas con fibras de vidrio

Cement pastes reinforced with glassfibre, popularly called GRC ("Glassfibre Reinforced Cements") are compound materials which have been widely used in the construction field for the last 30 years. Their good mechanical properties, lightness and resistance to corrosion and fire make them suitable in the process of replacing the fibrocements with asbestos base in numerous applications. For that reason, their use has been extended in recent years and they have mainly been used to manufacture panels for facades and interior decoration as well as, to a smaller extent, for plumbing installations, roofs, lost formwork and even for "fingers" at the airports (1, 2, 3, 4, 5). Nevertheless, it has been known for over 25 years that GRC suffer deterioration with the time which affects their mechanical properties and especially their tenacity; they become fragile and crack under small deformations. The research carried out to determine the causes of the phenomenon and look for possible solutions has been intense and it would be impossible to give more details here due to the lack of space. We will try, nevertheless, to summarize some more relevant aspects of the problem and the solutions that have reached the highest point of development so far.Las pastas de cemento reforzadas con fibras de vidrio, denominadas popularmente GRC ("Glassfibre Reinforced Cements"), son materiales compuestos que se utilizan ampliamente en el campo de la Construcción desde hace unos 30 años. Sus buenas propiedades mecánicas, ligereza y resistencia a la corrosión y al fuego les hacen idóneos en el proceso de sustitución de los fibrocementos de base amianto en numerosas aplicaciones. Por tal motivo, su empleo se ha extendido en los últimos años, utilizándose fundamentalmente para fabricar paneles de cierre de fachadas y decoración de interiores y en menor grado para tuberías, cubiertas, encofrados permanentes e incluso para "fingers" o brazos de acceso a los aviones en aeropuertos (1, 2, 3, 4, 5). Sin embargo, es un hecho conocido desde hace más de 25 años que el GRC sufre un deterioro con el tiempo, que afecta a sus propiedades mecánicas y muy especialmente a su tenacidad, volviéndose frágil y agrietándose bajo pequeñas deformaciones. Las investigaciones realizadas desde entonces para determinar las causas del fenómeno y buscar posibles soluciones al mismo han sido numerosísimas y sería imposible detallarlas aquí por razones de espacio. Trataremos no obstante de resumir los aspectos más relevantes del problema y las soluciones más extendidas hasta el momento

El efecto del humo de sílice y el metacaolín en el proceso de envejecimiento de los morteros de cemento reforzados con fibras de vidrio (GRC)

The deterioration of the mechanical properties of glassfibre reinforced concrete (GRC) over time rules out the use of this material in load-bearing structures. While one possible solution to this problem is the addition of pozzolans or metakaolin to the cement mortar, the amounts needed to ensure GRC integrity raise its price to non-competitive levels. Experimental research has been conducted to analyze whether the addition of small amounts of silica fume or metakaolin can prevent or mitigate the ageing issue. Unfortunately, the findings indicate that the addition of small proportions of metakaolin or silica fume to GRC are ineffective in improving its long-term performance.Para el uso del mortero de cemento reforzado con fibras de vidrio (GRC) en estructuras portantes se han de solucionar los problemas de reducción de las propiedades mecánicas que aparecen con el paso del tiempo. Estos problemas pueden ser solucionados mediante la adición de puzolanas o de metacaolín, a la pasta de mortero de cemento. Sin embargo, la cantidad de metacaolín que ha de ser añadida es elevada y el precio del GRC fabricado está fuera del mercado. Se ha realizado una campaña experimental que analiza si la adición de humo de sílice o de metacaolín en proporciones reducidas consigue evitar o paliar el problema del envejecimiento, que supone un freno al uso del GRC en elementos estructurales. Desgraciadamente, los resultados experimentales muestran que proporciones bajas de metacaolín o de humo de sílice no son efectivas para reducir el problema de pérdida de propiedades mecánicas

A systematic approach to evaluate metabolic conversions of amino acids in silac experiments

Comunicaciones a congreso

Efectos del género en la percepción de apoyo de las necesidades psicológicas básicas en educación física

El rol que ejerce el maestro de Educación Física en el desarrollo óptimo de los procesos motivacionales y en la promoción de la práctica de actividad física de niños y adolescentes es una temática de interés creciente en los últimos años. En este sentido, la adopción de estilos más activos y saludables en el tiempo de ocio constituye uno de los principales objetivos que rigen el curriculum ordinario. Así, siguiendo la Teoría de la Autodeterminación (Ryan & Deci, 2002) el objetivo del presente estudio fue analizar la percepción del apoyo de las necesidades psicológicas básicas (autonomía, competencia y relaciones sociales) de los discentes en las clases de Educación Física, en función del género. En el estudio participaron 158 alumnos de 10 a 12 años (M edad = 10.81, DT = 0.62), 76 chicos y 82 chicas y sus 6 maestros (3 varones y 3 mujeres) de Educación Física (M edad = 39.75; DT = 0.48). Para ello, se midió este antecedente social a través del Cuestionario de Apoyo a las Necesidades Psicológicas Básicas (Sánchez-Oliva, Leo, Sánchez-Miguel, Amado & García-Calvo, 2013). Los resultados señalan que los chicos presentan valores significativamente superiores que las chicas en la percepción de apoyo a la competencia y apoyo a la autonomía. Por tanto, se proponen como implicaciones prácticas que los docentes traten de apoyar los mediadores psicológicos mediante diferentes estrategias de intervención, haciendo hincapié en el género femenino. Para ello, la formación en teorías y estrategias motivacionales parece una de las líneas educativas prioritarias en los planes de formación docente

Neutron cross-sections for advanced nuclear systems : The n-TOF project at CERN

© Owned by the authors, published by EDP Sciences, 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedThe study of neutron-induced reactions is of high relevance in a wide variety of fields, ranging from stellar nucleosynthesis and fundamental nuclear physics to applications of nuclear technology. In nuclear energy, high accuracy neutron data are needed for the development of Generation IV fast reactors and accelerator driven systems, these last aimed specifically at nuclear waste incineration, as well as for research on innovative fuel cycles. In this context, a high luminosity Neutron Time Of Flight facility, n-TOF, is operating at CERN since more than a decade, with the aim of providing new, high accuracy and high resolution neutron cross-sections. Thanks to the features of the neutron beam, a rich experimental program relevant to nuclear technology has been carried out so far. The program will be further expanded in the near future, thanks in particular to a new high-flux experimental area, now under construction.Peer reviewedFinal Published versio

Proteomics profiling of vitreous humor reveals complement and coagulation components, adhesion factors, and neurodegeneration markers as discriminatory biomarkers of vitreoretinal eye diseases

Funding Information: This project was supported by the University of Beira Interior— Health Sciences Research Centre (CICS-UBI) supported by FEDER funds through the POCI—COMPETE 2020—Operational Programme Competitiveness and Internationalisation in Axis I—Strengthening research, technological development, and innovation Project (POCI-01- 0145-FEDER-007491). CNB-CSIC proteomics lab is a member of Proteored, PRB2-ISCIII and is supported by grant PT13/0001, of the PE I +D+i 2013–2016, funded by ISCIII and FEDER. Publisher Copyright: Copyright © 2023 Santos, Ciordia, Mesquita, Cruz, Sousa, Passarinha, Tomaz and Paradela.Introduction: Diabetic retinopathy (DR) and age-related macular degeneration (AMD) are leading causes of visual impairment and blindness in people aged 50 years or older in middle-income and industrialized countries. Anti-VEGF therapies have improved the management of neovascular AMD (nAMD) and proliferative DR (PDR), no treatment options exist for the highly prevalent dry form of AMD.  Methods: To unravel the biological processes underlying these pathologies and to find new potential biomarkers, a label-free quantitative (LFQ) method was applied to analyze the vitreous proteome in PDR (n=4), AMD (n=4) compared to idiopathic epiretinal membranes (ERM) (n=4).  Results and discussion: Post-hoc tests revealed 96 proteins capable of differentiating among the different groups, whereas 118 proteins were found differentially regulated in PDR compared to ERM and 95 proteins in PDR compared to dry AMD. Pathway analysis indicates that mediators of complement, coagulation cascades and acute phase responses are enriched in PDR vitreous, whilst proteins highly correlated to the extracellular matrix (ECM) organization, platelet degranulation, lysosomal degradation, cell adhesion, and central nervous system development were found underexpressed. According to these results, 35 proteins were selected and monitored by MRM (multiple reaction monitoring) in a larger cohort of patients with ERM (n=21), DR/PDR (n=20), AMD (n=11), and retinal detachment (n=13). Of these, 26 proteins could differentiate between these vitreoretinal diseases. Based on Partial least squares discriminant and multivariate exploratory receiver operating characteristic (ROC) analyses, a panel of 15 discriminatory biomarkers was defined, which includes complement and coagulation components (complement C2 and prothrombin), acute-phase mediators (alpha-1-antichymotrypsin), adhesion molecules (e.g., myocilin, galectin-3-binding protein), ECM components (opticin), and neurodegeneration biomarkers (beta-amyloid, amyloid-like protein 2).publishersversionpublishe