288 research outputs found
The Histone 3'-Terminal Stem-Loop-Binding Protein Enhances Translation through a Functional and Physical Interaction with Eukaryotic Initiation Factor 4G (eIF4G) and eIF3
Metazoan cell cycle-regulated histone mRNAs are unique cellular mRNAs in that they terminate in a highly conserved stem-loop structure instead of a poly(A) tail. Not only is the stem-loop structure necessary for 3'-end formation but it regulates the stability and translational efficiency of histone mRNAs. The histone stem-loop structure is recognized by the stem-loop-binding protein (SLBP), which is required for the regulation of mRNA processing and turnover. In this study, we show that SLBP is required for the translation of mRNAs containing the histone stem-loop structure. Moreover, we show that the translation of mRNAs ending in the histone stem-loop is stimulated in Saccharomyces cerevisiae cells expressing mammalian SLBP. The translational function of SLBP genetically required eukaryotic initiation factor 4E (eIF4E), eIF4G, and eIF3, and expressed SLBP coisolated with S. cerevisiae initiation factor complexes that bound the 5' cap in a manner dependent on eIF4G and eIF3. Furthermore, eIF4G coimmunoprecipitated with endogenous SLBP in mammalian cell extracts and recombinant SLBP and eIF4G coisolated. These data indicate that SLBP stimulates the translation of histone mRNAs through a functional interaction with both the mRNA stem-loop and the 5' cap that is mediated by eIF4G and eIF3
Le Libraire au Lecteur
C'est par hazard et non par dessein que j'ay formĂ© le Recueil des Pieces curieuses que je prĂ©sente au Public. Je nâavois dâabord songĂ© quâĂ donner la premiĂšre traduction de la Conjecture du cardinal de Cusa qui mâavoit Ă©tĂ© remise entre les mains, avec lâAvertissement et les remarques dâun savant thĂ©ologien, qui avoit travaillĂ© Ă cette traduction. Mais lors que ce petit ouvrage fut imprimĂ©, quelques personnes qui en avoient eu connoissance prirent soin de me faire tomber entre les mains la sec..
BiodiversitĂ© et territoire viticole : Ă©tude des interactions entre viticulture et biodiversitĂ© dans le cadre dâune initiative locale Ă lâĂ©chelle de lâAOC Saumur-Champigny
Genome-wide analysis of adolescent psychotic-like experiences shows genetic overlap with psychiatric disorders
This study aimed to test for overlap in genetic influences between psychotic-like experience traits shown by adolescents in the community, and clinically-recognized psychiatric disorders in adulthood, specifically schizophrenia, bipolar disorder, and major depression. The full spectra of psychotic-like experience domains, both in terms of their severity and type (positive, cognitive, and negative), were assessed using self- and parent-ratings in three European community samples aged 15-19 years (Final N incl. siblings = 6,297-10,098). A mega-genome-wide association study (mega-GWAS) for each psychotic-like experience domain was performed. SNP-heritability of each psychotic-like experience domain was estimated using genomic-relatedness-based restricted maximum-likelihood (GREML) and linkage disequilibrium- (LD-) score regression. Genetic overlap between specific psychotic-like experience domains and schizophrenia, bipolar disorder, and major depression was assessed using polygenic risk scoring (PRS) and LD-score regression. GREML returned SNP-heritability estimates of 3-9% for psychotic-like experience trait domains, with higher estimates for less skewed traits (Anhedonia, Cognitive Disorganization) than for more skewed traits (Paranoia and Hallucinations, Parent-rated Negative Symptoms). Mega-GWAS analysis identified one genome-wide significant association for Anhedonia within IDO2 but which did not replicate in an independent sample. PRS analysis revealed that the schizophrenia PRS significantly predicted all adolescent psychotic-like experience trait domains (Paranoia and Hallucinations only in non-zero scorers). The major depression PRS significantly predicted Anhedonia and Parent-rated Negative Symptoms in adolescence. Psychotic-like experiences during adolescence in the community show additive genetic effects and partly share genetic influences with clinically-recognized psychiatric disorders, specifically schizophrenia and major depression
Reports to the UK Yellow Card Scheme 2000-2009 for medications used in paediatric rheumatology
Real estate investment and urban density:exploring the polycentric urban region using a topological lens
Focusing on commercial office real estate as both a manifestation of and a conduit of cross-border capital ïŹows, this paper refers to the concepts of topology and topography in a theoretical and empirical exploration of contemporary ânetwork economyâ spatial implications for the âpolycentric urban regionâ (PUR). A body of research has cast doubt on the normative European representation of the multi-centre PUR as a balanced, sustainable spatial development model. Yet, the model has continued to be propagated in European territorial strategy and has been influential internationally. Academic perspectives and qualitative evidence reviewed in the paper shed a light on mutual dependencies and recursive relations between network economy global structural processes, international office real estate investment practices mediated by city governments, and the spatial configuration of density. Commercial investment and city planning actor practices chime with urban agglomeration, spatial concentration and density. Quantitative evidence of associations between urban density and office real estate investment returns and capital flows is found. It is concluded that network economy topology, politics and the city are in a dialectical relationship with the PUR territorial governance agenda for spatially balanced regional development.</p
Quelles distributions des espĂšces et des traits dans des espaces herbacĂ©s ordinaires le long dâun gradient ville â campagne ?
International audienc
The Rio de la Plata craton and the adjoining Pan-African/brasiliano terranes: Their origins and incorporation into south-west Gondwana *
The Carnegie Supernova Project: First Near-Infrared Hubble Diagram to z~0.7
The Carnegie Supernova Project (CSP) is designed to measure the luminosity
distance for Type Ia supernovae (SNe Ia) as a function of redshift, and to set
observational constraints on the dark energy contribution to the total energy
content of the Universe. The CSP differs from other projects to date in its
goal of providing an I-band {rest-frame} Hubble diagram. Here we present the
first results from near-infrared (NIR) observations obtained using the Magellan
Baade telescope for SNe Ia with 0.1 < z < 0.7. We combine these results with
those from the low-redshift CSP at z <0.1 (Folatelli et al. 2009). We present
light curves and an I-band Hubble diagram for this first sample of 35 SNe Ia
and we compare these data to 21 new SNe Ia at low redshift. These data support
the conclusion that the expansion of the Universe is accelerating. When
combined with independent results from baryon acoustic oscillations (Eisenstein
et al. 2005), these data yield Omega_m = 0.27 +/- 0.0 (statistical), and
Omega_DE = 0.76 +/- 0.13 (statistical) +/- 0.09 (systematic), for the matter
and dark energy densities, respectively. If we parameterize the data in terms
of an equation of state, w, assume a flat geometry, and combine with baryon
acoustic oscillations, we find that w = -1.05 +/- 0.13 (statistical) +/- 0.09
(systematic). The largest source of systematic uncertainty on w arises from
uncertainties in the photometric calibration, signaling the importance of
securing more accurate photometric calibrations for future supernova cosmology
programs. Finally, we conclude that either the dust affecting the luminosities
of SNe Ia has a different extinction law (R_V = 1.8) than that in the Milky Way
(where R_V = 3.1), or that there is an additional intrinsic color term with
luminosity for SNe Ia independent of the decline rate.Comment: 44 pages, 23 figures, 9 tables; Accepted for publication in the
Astrophysical Journa
Interfacial chemistry effects in the electrochemical performance of silicon electrodes under lithiumâion battery conditions
Understanding the solid electrolyte interphase (SEI) formation and (de)lithiation phenomena at silicon (Si) electrodes is key to improving the performance and lifetime of Siâbased lithiumâion batteries. However, these processes remain somewhat elusive, and, in particular, the role of Si surface termination merits further consideration. Here, scanning electrochemical cell microscopy (SECCM) is used in a glovebox, followed by secondary ion mass spectrometry (SIMS) at identical locations to study the local electrochemical behavior and associated SEI formation, comparing Si (100) with a native oxide layer (SiO x /Si) and etched with hydrofluoric acid (HFâSi). HFâSi shows greater spatial electrochemical heterogeneity and inferior lithiation reversibility than SiO x /Si. This is attributed to a weakly passivating SEI and irreversible lithium trapping at the Si surface. Combinatorial screening of charge/discharge cycling by SECCM with coâlocated SIMS reveals SEI chemistry as a function of depth. While the SEI thickness is relatively independent of the cycle number, the chemistry â particularly in the intermediate layers â depends on the number of cycles, revealing the SEI to be dynamic during cycling. This work serves as a foundation for the use of correlative SECCM/SIMS as a powerful approach to gain fundamental insights on complex battery processes at the nanoâ and microscales
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