Protein kinase C is a calcium sensor for presynaptic short-term plasticity

In presynaptic boutons, calcium (Ca2+) triggers both neurotransmitter release and short-term synaptic plasticity. Whereas synaptotagmins are known to mediate vesicle fusion through binding of high local Ca2+ to their C2 domains, the proteins that sense smaller global Ca2+ increases to produce short-term plasticity have remained elusive. Here, we identify a Ca2+ sensor for post-tetanic potentiation (PTP), a form of plasticity thought to underlie short-term memory. We find that at the functionally mature calyx of Held synapse the Ca2+-dependent protein kinase C isoforms α and β are necessary for PTP, and the expression of PKCβ in PKCαβ double knockout mice rescues PTP. Disruption of Ca2+ binding to the PKCβ C2 domain specifically prevents PTP without impairing other PKCβ-dependent forms of synaptic enhancement. We conclude that different C2-domain-containing presynaptic proteins are engaged by different Ca2+ signals, and that Ca2+ increases evoked by tetanic stimulation are sensed by PKCβ to produce PTP. DOI: http://dx.doi.org/10.7554/eLife.03011.00

Cooling Flow Star Formation and the Apparent Stellar Ages of Elliptical Galaxies

Observational constraints and theoretical arguments indicate that cooled interstellar gas in bright elliptical galaxies forms into a young stellar population throughout the region within the half-light radius. The young population has a bottom-heavy, but optically luminous IMF extending to 1 - 2 M_sun. When the colors and spectral features of this young population are combined with those of the underlying old stellar population, the apparent ages are significantly reduced, similar to the relatively young apparent ages observed in many ellipticals. Galactic mergers are not required to resupply young stars. The sensitivity of continuous star formation to L_B and L_x/L_B is likely to account for the observed spread in apparent ages among elliptical galaxies. Local star formation is accompanied by enhanced stellar H_beta equivalent widths, stronger optical emission lines, enhanced thermal X-ray emission and lower apparent temperatures in the hot gas. The young stars should cause M/L to vary with galactic radius, perturbing the fundamental plane occupied by the old stars.Comment: 6 pages with 2 figures; accepted by Astrophysical Journal Letter

BVRI Surface Photometry of Isolated Spiral Galaxies

A release of multicolor broad band (BVRI) photometry for a subsample of 44 isolated spirals drawn from the Catalogue of Isolated Galaxies (CIG) is presented. Total magnitudes and colors at various circular apertures, as well as some global structural/morphological parameters are estimated. Morphology is reevaluated through optical and sharp/filtered R band images, (B-I) color index maps, and archive near-IR JHK images from the Two-Micron Survey. The CAS structural parameters (Concentration, Asymmetry, and Clumpiness) were calculated from the images in each one of the bands. The fraction of galaxies with well identified optical/near-IR bars (SB) is 63%, while a 17% more shows evidence of weak or suspected bars (SAB). The sample average value of the maximum bar ellipticity is 0.4. Half of the galaxies in the sample shows rings. We identify two candidates for isolated galaxies with disturbed morphology. The structural CAS parameters change with the observed band, and the tendencies they follow with the morphological type and global color are more evident in the redder bands. In any band, the major difference between our isolated spirals and a sample of interacting spirals is revealed in the A-S plane. A deep and uniformly observed sample of isolated galaxies is intended for various purposes including (i) comparative studies of environmental effects, (ii) confronting model predictions of galaxy evolution and (iii) evaluating the change of galaxy properties with redshift.Comment: 44 pages, 9 figures and 7 tables included. To appear in The Astronomical Journal. For the 43 appendix figures 4.1-4.43 see http://www.astroscu.unam.mx/~avila/Figs4.1_4.43.tar.gz (7.2 Mb tar.gz file

Radial Distribution of the Mass-to-Luminosity Ratio in Spiral Galaxies and Massive Dark Cores

We derive radial profiles of the surface-mass-density for 19 spiral galaxies directly from their high-resolution rotation curves. Using the corresponding luminosity profiles, we obtain the radial distribution of the mass-to-luminosity ratios ($M/L$) from the inner bulge ($\sim$ a few 100 pc) to the outer disk ($\geq$ 2-10 kpc) for 11 galaxies (with inclination < $70^{\circ}$ in order to reduce the influence of the interstellar extinction. The $M/L$s in the bulges of two galaxies with sufficient resolution, NGC 4527 and NGC 6946, are found to increase steeply toward the center at radii $\sim$ 100-500 pc at rates of 15$\pm$3 and 7$\pm$2 times per kpc, respectively. Some other galaxies with fairly high resolution also show signs of an increase toward the center. Such an increase may indicate the existence of a new component, a ``massive dark core'', which may be an object linking the bulge and a central black hole. Based on radial variations of the $M/L$, we further discuss the variation of the dark-mass fraction in spiral galaxies.Comment: Latex 19 pages, 30 ps figures. ApJ in pres

Spin accumulation induced resistance in mesoscopic ferromagnet/ superconductor junctions

We present a description of spin-polarized transport in mesoscopic ferromagnet-superconductor (F/S) systems, where the transport is diffusive, and the interfaces are transparent. It is shown that the spin reversal associated with Andreev reflection generates an excess spin density close to the F/S interface, which leads to a spin contact resistance. Expressions for the contact resistance are given for two terminal and four terminal geometries. In the latter the sign depends on the relative magnetization of the ferromagnetic electrodes.Comment: RevTeX 10 pages, 4 figures, submitted to Phys.Rev. Let

Quantum shot-noise at local tunneling contacts on mesoscopic multiprobe conductors

New experiments that measure the low-frequency shot-noise spectrum at local tunneling contacts on mesoscopic structures are proposed. The current fluctuation spectrum at a single tunneling tip is determined by local partial densities of states. The current-correlation spectrum between two tunneling tips is sensitive to non-diagonal density of states elements which are expressed in terms of products of scattering states of the conductor. Thus such an experiment permits to investigate correlations of electronic wave functions. We present specific results for a clean wire with a single barrier and for metallic diffusive conductors.Comment: 4 pages REVTeX, 2 figure

Patients with diffuse idiopathic skeletal hyperostosis have an increased burden of thoracic aortic calcifications

OBJECTIVES: DISH has been associated with increased coronary artery calcifications and incident ischaemic stroke. The formation of bone along the spine may share pathways with calcium deposition in the aorta. We hypothesized that patients with DISH have increased vascular calcifications. Therefore we aimed to investigate the presence and extent of DISH in relation to thoracic aortic calcification (TAC) severity. METHODS: This cross-sectional study included 4703 patients from the Second Manifestation of ARTerial disease cohort, consisting of patients with cardiovascular events or risk factors for cardiovascular disease. Chest radiographs were scored for DISH using the Resnick criteria. Different severities of TAC were scored arbitrarily from no TAC to mild, moderate or severe TAC. Using multivariate logistic regression, the associations between DISH and TAC were analysed with adjustments for age, sex, BMI, diabetes, smoking status, non-high-density lipoprotein cholesterol, cholesterol lowering drug usage, renal function and blood pressure. RESULTS: A total of 442 patients (9.4%) had evidence of DISH and 1789 (38%) patients had TAC. The prevalence of DISH increased from 6.6% in the no TAC group to 10.8% in the mild, 14.3% in the moderate and 17.1% in the severe TAC group. After adjustments, DISH was significantly associated with the presence of TAC [odds ratio (OR) 1.46 [95% CI 1.17, 1.82)]. In multinomial analyses, DISH was associated with moderate TAC [OR 1.43 (95% CI 1.06, 1.93)] and severe TAC [OR 1.67 (95% CI 1.19, 2.36)]. CONCLUSIONS: Subjects with DISH have increased TACs, providing further evidence that patients with DISH have an increased burden of vascular calcifications

Internal medicine specialists' attitudes towards working part-time: a comparison between 1996 and 2004

BACKGROUND: Although medical specialists traditionally hold negative views towards working part-time, the practice of medicine has evolved. Given the trend towards more part-time work and that there is no evidence that it compromises the quality of care, attitudes towards part-time work may have changed as well in recent years. The aim of this paper was to examine the possible changes in attitudes towards part-time work among specialists in internal medicine between 1996 and 2004. Moreover, we wanted to determine whether these attitudes were associated with individual characteristics (age, gender, investments in work) and whether attitudes of specialists within a partnership showed more resemblance than specialists' attitudes from different partnerships. METHODS: Two samples were used in this study: data of a survey conducted in 1996 and in 2004. After selecting internal medicine specialists working in general hospitals in The Netherlands, the sample consisted of 219 specialists in 1996 and 363 specialists in 2004. They were sent a questionnaire, including topics on the attitudes towards part-time work. RESULTS: Internal medicine specialists' attitudes towards working part-time became slightly more positive between 1996 and 2004. Full-time working specialists in 2004 still expressed concerns regarding the investments of part-timers in overhead tasks, the flexibility of task division, efficiency, communication and continuity of care. In 1996 gender was the only predictor of the attitude, in 2004 being a full- or a part-timer, age and the time invested in work were associated with this attitude. Furthermore, specialists' attitudes were not found to cluster much within partnerships. CONCLUSION: In spite of the increasing number of specialists working or preferring to work part-time, part-time practice among internal medicine specialists seems not to be fully accepted. The results indicate that the attitudes are no longer gender based, but are associated with age and work aspects such as the number of hours worked. Though there is little evidence to support them, negative ideas about the consequences of part-time work for the quality of care still exist. Policy should be aimed at removing the organisational difficulties related to part-time work and create a system in which part-time practice is fully integrated and accepted

The POINT-AGAPE survey II: An Unrestricted Search for Microlensing Events towards M31

An automated search is carried out for microlensing events using a catalogue of 44554 variable superpixel lightcurves derived from our three-year monitoring program of M31. Each step of our candidate selection is objective and reproducible by a computer. Our search is unrestricted, in the sense that it has no explicit timescale cut. So, it must overcome the awkward problem of distinguishing long-timescale microlensing events from long-period stellar variables. The basis of the selection algorithm is the fitting of the superpixel lightcurves to two different theoretical models, using variable star and blended microlensing templates. Only if microlensing is preferred is an event retained as a possible candidate. Further cuts are made with regard to (i) sampling, (ii) goodness of fit of the peak to a Paczynski curve, (iii) consistency of the microlensing hypothesis with the absence of a resolved source, (iv) achromaticity, (v) position in the colour-magnitude diagram and (vi) signal-to-noise ratio. Our results are reported in terms of first-level candidates, which are the most trustworthy, and second-level candidates, which are possible microlensing but have lower signal-to-noise and are more questionable. The pipeline leaves just 3 first-level candidates, all of which have very short full-width half-maximum timescale (<5 days) and 3 second-level candidates, which have timescales of 31, 36 and 51 days respectively. We also show 16 third-level lightcurves, as an illustration of the events that just fail the threshold for designation as microlensing candidates. They are almost certainly mainly variable stars. Two of the 3 first-level candidates correspond to known events (PA 00-S3 and PA 00-S4) already reported by the POINT-AGAPE project. The remaining first-level candidate is new.Comment: 22 pages, 18 figures, MNRAS, to appea

Microlensing towards LMC: a study of the LMC halo contribution

We carry on a new analysis of the sample of MACHO microlensing candidates towards the LMC. Our main purpose is to determine the lens population to which the events may belong. We give particular emphasis to the possibility of characterizing the Milky Way dark matter halo population with respect to the LMC one. Indeed, we show that only a fraction of the events have characteristics that match those expected for lenses belonging to the MACHO population of the Milky Way halo. This suggests that this component cannot explain all the candidates. Accordingly, we challenge the view that the dark matter halo fraction of both the Milky Way and the LMC halos are equal, and indeed we show that, for a MACHO mass in the range 0.1-0.3 M$_\odot$, the LMC halo fraction can be significantly larger than the Milky Way one. In this perspective, our main conclusion is that up to about half of the observed events could be attributed to the LMC MACHO dark matter halo.Comment: Accepted for publication in Astronomy & Astrophysic