Impact of sheep grazing on juvenile sea bass, Dicentrarchus labrax L., in tidal salt marshes

The diet of young of the year sea bass, Dicentrarchus labrax L., from sheep grazed and ungrazed tidal salt marshes were com-pared qualitatively and quantitatively in Mont Saint-Michel Bay. In areas without grazing pressure, the vegetation gradient changes from a pioneer Puccinellia maritima dominated community at the tidal ¯at boundaries through a Atriplex portulacoides dominated community in the middle of the marsh to a mature Elymus pungens dominated community at the landward edge. The A. portula-coides community is highly productive and provides important quantities of litter which provides a habitat and good supply to substain high densities of the detrivorous amphipod Orchestia gammarellus. In the grazed areas, the vegetation is replaced by P. maritima communities, a low productive grass plant, and food availability and habitat suitability are reduced for O. gammarellus. Juvenile sea bass colonise the salt marsh at ¯ood during 43% of the spring tides which inundate the salt marsh creeks. They forage inside the marsh and feed mainly on O. gammarellus in the ungrazed marshes. In grazed areas, this amphipod is replaced by other species and juvenile sea bass consume less food from the marsh. This illustrates a direct effect of a terrestrial herbivore on a coastal food web, and suggests that management of salt marsh is complex and promotion of one component of their biota could involve reductions in other species

Biodiversity and species interactions: extending Lotka-Volterra community theory

A new analysis of the nearly century-old Lotka-Volterra theory allows us to link species interactions to biodiversity patterns, including: species abundance distributions, estimates of total community size, patterns of community invasibility, and predicted responses to disturbance. Based on a few restrictive assumptions about species interactions, our calculations require only that the community is sufficiently large to allow a mean-field approximation. We develop this analysis to show how an initial assemblage of species with varying interaction strengths is predicted to sort out into the final community based on the species' predicted target densities. The sorting process yields predictions of covarying patterns of species abundance, community size, and species interaction strengths. These predictions can be tested using enrichment experiments, examination of latitudinal and productivity gradients, and features of community assembly

The evolution of ecological specialization across the range of a broadly distributed marine species

Ecological specialization is an important engine of evolutionary change and adaptive radiation, but empirical evidence of local adaptation in marine environments is rare, a pattern that has been attributed to the high dispersal ability of marine taxa and limited geographic barriers to gene flow. The broad-nosed pipefish, Syngnathus typhle, is one of the most broadly distributed syngnathid species and shows pronounced variation in cranial morphology across its range, a factor that may contribute to its success in colonizing new environments. We quantified variation in cranial morphology across the species range using geometric morphometrics, and tested for evidence of trophic specialization by comparing individual-level dietary composition with the community of prey available at each site. Although the diets of juvenile pipefish from each site were qualitatively similar, ontogenetic shifts in dietary composition resulted in adult populations with distinctive diets consistent with their divergent cranial morphology. Morphological differences found in nature are maintained under common garden conditions, indicating that trophic specialization in S. typhle is a heritable trait subject to selection. Our data highlight the potential for ecological specialization in response to spatially variable selection pressures in broadly distributed marine species.Swiss Academy of SciencesSwiss National Science FoundationSwiss National Science Foundation (SNSF)European CommissionUniversity of ZurichBrooklyn CollegeCity University of New Yorkinfo:eu-repo/semantics/acceptedVersio

Global Patterns of Guild Composition and Functional Diversity of Spiders

The objectives of this work are: (1) to define spider guilds for all extant families worldwide; (2) test if guilds defined at family level are good surrogates of species guilds; (3) compare the taxonomic and guild composition of spider assemblages from different parts of the world; (4) compare the taxonomic and functional diversity of spider assemblages and; (5) relate functional diversity with habitat structure. Data on foraging strategy, prey range, vertical stratification and circadian activity was collected for 108 families. Spider guilds were defined by hierarchical clustering. We searched for inconsistencies between family guild placement and the known guild of each species. Richness and abundance per guild before and after correcting guild placement were compared, as were the proportions of each guild and family between all possible pairs of sites. Functional diversity per site was calculated based on hierarchical clustering. Eight guilds were discriminated: (1) sensing, (2) sheet, (3) space, and (4) orb web weavers; (5) specialists; (6) ambush, (7) ground, and (8) other hunters. Sixteen percent of the species richness corresponding to 11% of all captured individuals was incorrectly attributed to a guild by family surrogacy; however, the correlation of uncorrected vs. corrected guilds was invariably high. The correlation of guild richness or abundances was generally higher than the correlation of family richness or abundances. Functional diversity was not always higher in the tropics than in temperate regions. Families may potentially serve as ecological surrogates for species. Different families may present similar roles in the ecosystems, with replacement of some taxa by other within the same guild. Spiders in tropical regions seem to have higher redundancy of functional roles and/or finer resource partitioning than in temperate regions. Although species and family diversity were higher in the tropics, functional diversity seems to be also influenced by altitude and habitat structure

Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel Production

The epidemic of bovine spongiform encephalopathy (BSE) has led to a world-wide drop in the market for beef by-products, such as Meat-and-Bone Meal (MBM), a fat-containing but mainly proteinaceaous product traditionally used as an animal feed supplement. While normal rendering is insufficient, the production of biodiesel from MBM has been suggested to destroy infectivity from transmissible spongiform encephalopathies (TSEs). In addition to producing fuel, this method simultaneously generates a nutritious solid residue. In our study we produced biodiesel from MBM under defined conditions using a modified form of alkaline methanolysis. We evaluated the presence of prion in the three resulting phases of the biodiesel reaction (Biodiesel, Glycerol and Solid Residue) in vitro and in vivo. Analysis of the reaction products from 263K scrapie infected MBM led to no detectable immunoreactivity by Western Blot. Importantly, and in contrast to the biochemical results the solid MBM residue from the reaction retained infectivity when tested in an animal bioassay. Histochemical analysis of hamster brains inoculated with the solid residue showed typical spongiform degeneration and vacuolation. Re-inoculation of these brains into a new cohort of hamsters led to onset of clinical scrapie symptoms within 75 days, suggesting that the specific infectivity of the prion protein was not changed during the biodiesel process. The biodiesel reaction cannot be considered a viable prion decontamination method for MBM, although we observed increased survival time of hamsters and reduced infectivity greater than 6 log orders in the solid MBM residue. Furthermore, results from our study compare for the first time prion detection by Western Blot versus an infectivity bioassay for analysis of biodiesel reaction products. We could show that biochemical analysis alone is insufficient for detection of prion infectivity after a biodiesel process

Security Definitions For Hash Functions: Combining UCE and Indifferentiability

Hash functions are one of the most important cryptographic primitives, but their desired security properties have proven to be remarkably hard to formalize. To prove the security of a protocol using a hash function, nowadays often the random oracle model (ROM) is used due to its simplicity and its strong security guarantees. Moreover, hash function constructions are commonly proven to be secure by showing them to be indifferentiable from a random oracle when using an ideal compression function. However, it is well known that no hash function realizes a random oracle and no real compression function realizes an ideal one. As an alternative to the ROM, Bellare et al. recently proposed the notion of universal computational extractors (UCE). This notion formalizes that a family of functions ``behaves like a random oracle\u27\u27 for ``real-world\u27\u27 protocols while avoiding the general impossibility results. However, in contrast to the indifferentiability framework, UCE is formalized as a multi-stage game without clear composition guarantees. As a first contribution, we introduce context-restricted indifferentiability (CRI), a generalization of indifferentiability that allows us to model that the random oracle does not compose generally but can only be used within a well-specified set of protocols run by the honest parties, thereby making the provided composition guarantees explicit. We then show that UCE and its variants can be phrased as a special case of CRI. Moreover, we show how our notion of CRI leads to generalizations of UCE. As a second contribution, we prove that the hash function constructed by Merkle-Damgard satisfies one of the well-known UCE variants, if we assume that the compression function satisfies one of our generalizations of UCE, basing the overall security on a plausible assumption. This result further validates the Merkle-Damgard construction and shows that UCE-like assumptions can serve both as a valid reference point for modular protocol analyses, as well as for the design of hash functions, linking those two aspects in a framework with explicit composition guarantees

Evidence of niche partitioning under ontogenetic influences among three morphologically similar siluriformes in small subtropical streams

Ontogenetic influences in patterns of niche breadth and feeding overlap were investigated in three species of Siluriformes (Heptapterus sp., Rhamdia quelen and Trichomycterus poikilos) aiming at understanding the species coexistence. Samplings were conducted bimonthly by electrofishing technique from June/2012 to June/2013 in ten streams of the northwestern state of Rio Grande do Sul, Brazil. The stomach contents of 1,948 individuals were analyzed by volumetric method, with 59 food items identified. In general Heptapterus sp. consumed a high proportion of Aegla sp., terrestrial plant remains and Megaloptera; R. quelen consumed fish, and Oligochaeta, followed by Aegla sp.; while the diet of T. poikilos was based on Simuliidae, Ephemeroptera and Trichoptera. Specie segregation was observed in the NMDS. Through PERMANOVA analysis feeding differences among species, and between a combination of species plus size classes were observed. IndVal showed which items were indicators of these differences. Niche breadth values were high for all species. The niche breadth values were low only for the larger size of R. quelen and Heptapterus sp. while T. poikilos values were more similar. Overall the species were a low feeding overlap values. The higher frequency of high feeding overlap was observed for interaction between Heptapterus sp. and T. poikilos. The null model confirmed the niche partitioning between the species. The higher frequency of high and intermediate feeding overlap values were reported to smaller size classes. The null model showed resource sharing between the species/size class. Therefore, overall species showed a resource partitioning because of the use of occasional items. However, these species share resources mainly in the early ontogenetic stages until the emphasized change of morphological characteristics leading to trophic niche expansion and the apparent segregation observed

Effects of varying organic matter content on the development of green roof vegetation: a six year experiment

Green roofs can potentially be used to tackle a variety of environmental problems, and can be used as development mitigation for the loss of ground-based habitats. Brown (biodiversity) roofs are a type of green roof designed to imitate brownfield habitat, but the best way of engineering these habitats requires more research. We tested the effects of altering organic matter content on the development of vegetation assemblages of experimental brown (biodiversity) roof mesocosms. Three mulch treatments were tested: (1) Sandy loam, where 10mm of sandy loam mulch (about 3% organic matter by dry weight) was added to 100mm of recycled aggregate; (2) Compost, where the mulch also contained some garden compost (about 6% organic matter by dry weight); and (3) No mulch, where no mulch was added. Mesocosms were seeded with a wildflower mix that included some Sedum acre, and vegetation development was investigated over a six-year period. Species richness, assemblage character, number of plants able to seed, and above-ground plant biomass were measured. Drought disturbance was an important control on plant assemblages in all mulch treatments, but there were significant treatment response interactions. The more productive Compost treatment was associated with larger plant coverage and diversity before the occurrence of a sequence of drought disturbances, but was more strongly negatively affected by the disturbances than the two less productive treatments. We suggest that this was due to the over-production of plant biomass in the more productive treatment, which made the plants more vulnerable to the effects of drought disturbance, leading to a kind of 'boom-bust' assemblage dynamic. The 'ideal' amount of added organic matter for these green roof systems was very low, but other types of green roof that have a larger water holding capacity, and/or more drought resistant plant floras, will likely require more organic matter or fertiliser. Nonetheless, nutrient-supported productivity in green roof systems should be kept low in order to avoid boom-bust plant assemblage dynamics. Research into the best way of engineering green roof habitats should take place over a long enough multi-year time period to include the effects of temporally infrequent disturbances