A SOLAR-POWERED WHITE LED-BASED UV-VIS SPECTROPHOTOMETRIC SYSTEM MANAGED BY PC FOR AIR POLLUTION DETECTION IN FARAWAY AND UNFRIENDLY LOCATIONS

This research work regards the design and realization of an absorption spectrophotometer based on a LED light source in place of the usually employed Xenon lamp. The advantage of the use of LED technology resides in several factors such as the reducing of the analyte temperature variations and thus noise generation, which occur if a Xenon light source is used, beside of the high luminous efficiency, reliability, operating duration, lower maintenance and a lower power consumption. This last factor allows to supply the entire designed apparatus using a solar panel thus making the system easly portable for use even in places where the electricity network is absent. An optical filtering system was realized in order to detect the analyte absorption for each wavelength range selected by the optical filters. A PC-interfaced PIC-based control unit used to manage the different functionalities required by the spectophotometer was realized and tested. The control unit acquires and processes, via the developed firmware, the raw data provided by different sensors employed in the system. The sensors are used to monitor analyte temperature and humidity values, to control the analyte pressure and to acquire the luminous intensity value of the light beam before and after passing through the analyte. Finally, the realized electronic control unit actuates different mechanical sections (stepper motor, solenoid valve), sincronyzing and controlling the data exchange between hardware sections, microcontroller and the PC

Macrophage migration inhibitory factor downregulation: a novel mechanism of resistance to anti-angiogenic therapy.

Anti-angiogenic therapies for cancer such as VEGF neutralizing antibody bevacizumab have limited durability. While mechanisms of resistance remain undefined, it is likely that acquired resistance to anti-angiogenic therapy will involve alterations of the tumor microenvironment. We confirmed increased tumor-associated macrophages in bevacizumab-resistant glioblastoma patient specimens and two novel glioblastoma xenograft models of bevacizumab resistance. Microarray analysis suggested downregulated macrophage migration inhibitory factor (MIF) to be the most pertinent mediator of increased macrophages. Bevacizumab-resistant patient glioblastomas and both novel xenograft models of resistance had less MIF than bevacizumab-naive tumors, and harbored more M2/protumoral macrophages that specifically localized to the tumor edge. Xenografts expressing MIF-shRNA grew more rapidly with greater angiogenesis and had macrophages localizing to the tumor edge which were more prevalent and proliferative, and displayed M2 polarization, whereas bevacizumab-resistant xenografts transduced to upregulate MIF exhibited the opposite changes. Bone marrow-derived macrophage were polarized to an M2 phenotype in the presence of condition-media derived from bevacizumab-resistant xenograft-derived cells, while recombinant MIF drove M1 polarization. Media from macrophages exposed to bevacizumab-resistant tumor cell conditioned media increased glioma cell proliferation compared with media from macrophages exposed to bevacizumab-responsive tumor cell media, suggesting that macrophage polarization in bevacizumab-resistant xenografts is the source of their aggressive biology and results from a secreted factor. Two mechanisms of bevacizumab-induced MIF reduction were identified: (1) bevacizumab bound MIF and blocked MIF-induced M1 polarization of macrophages; and (2) VEGF increased glioma MIF production in a VEGFR2-dependent manner, suggesting that bevacizumab-induced VEGF depletion would downregulate MIF. Site-directed biopsies revealed enriched MIF and VEGF at the enhancing edge in bevacizumab-naive patients. This MIF enrichment was lost in bevacizumab-resistant glioblastomas, driving a tumor edge M1-to-M2 transition. Thus, bevacizumab resistance is driven by reduced MIF at the tumor edge causing proliferative expansion of M2 macrophages, which in turn promotes tumor growth

Magnetic Field Induced Spin Polarization of AlAs Two-dimensional Electrons

Two-dimensional (2D) electrons in an in-plane magnetic field become fully spin polarized above a field B_P, which we can determine from the in-plane magnetoresistance. We perform such measurements in modulation-doped AlAs electron systems, and find that the field B_P increases approximately linearly with 2D electron density. These results imply that the product |g*|m*, where g* is the effective g-factor and m* the effective mass, is a constant essentially independent of density. While the deduced |g*|m* is enhanced relative to its band value by a factor of ~ 4, we see no indication of its divergence as 2D density approaches zero. These observations are at odds with results obtained in Si-MOSFETs, but qualitatively confirm spin polarization studies of 2D GaAs carriers.Comment: 4 pages, 5 figure

Anomalous spin-splitting of two-dimensional electrons in an AlAs Quantum Well

We measure the effective Lande g-factor of high-mobility two-dimensional electrons in a modulation-doped AlAs quantum well by tilting the sample in a magnetic field and monitoring the evolution of the magnetoresistance oscillations. The data reveal that |g| = 9.0, which is much enhanced with respect to the reported bulk value of 1.9. Surprisingly, in a large range of magnetic field and Landau level fillings, the value of the enhanced g-factor appears to be constant.Comment: 4 pages, 3 figure

Neuropeptide AF and FF modulation of adipocyte metabolism. Primary insights from functional genomics and effects on beta-adrenergic responsiveness.

The presence of a neuropeptide AF and FF receptor (NPFF-R2) mRNA in human adipose tissue (Elshourbagy, N. A., Ames, R. S., Fitzgerald, L. R., Foley, J. J., Chambers, J. K., Szekeres, P. G., Evans, N. A., Schmidt, D. B., Buckley, P. T., Dytko, G. M., Murdock, P. R., Tan, K. B., Shabon, U., Nuthulaganti, P., Wang, D. Y., Wilson, S., Bergsma, D. J., and Sarau, H. M. (2000) J. Biol. Chem. 275, 25965-25971) suggested these peptides, principally recognized for their pain modulating effects, may also impact on adipocyte metabolism, an aspect that has not been explored previously. Our aim was thus to obtain more insights into the actions of these peptides on adipocytes, an approach initially undertaken with a functional genomic assay. First we showed that 3T3-L1 adipocytes express both NPFF-R1 and NPFF-R2 transcripts, and that NPAF binds adipocyte membranes with a nanomolar affinity as assessed by surface plasmon resonance technology. Then, and following a 24-h treatment with NPFF or NPAF (1 microm), we have measured using real-time quantitative reverse transcriptase-PCR the mRNA steady state levels of already well characterized genes involved in key pathways of adipose metabolism. Among the 45 genes tested, few were modulated by NPFF ( approximately 10%) and a larger number by NPAF ( approximately 27%). Interestingly, NPAF increased the mRNA levels of beta2- and beta3-adrenergic receptors (AR), and to a lesser extent those of beta1-ARs. These variations in catecholamine receptor mRNAs correlated with a clear induction in the density of beta2- and beta3-AR proteins, and in the potency of beta-AR subtype-selective agonists to stimulate adenylyl cyclase activity. Altogether, these data show that NPFF-R1 and NPFF-R2 are functionally present in adipocytes and suggest that besides their well described pain modulation effects, NPAF and to a lesser extent NPFF, may have a global impact on body energy storage and utilization

Strongly induced collapse in the Class 0 protostar NGC 1333 IRAS 4A

The onset of gravitational collapse in cluster-forming clouds is still poorly known. Our goal is to use the Class 0 protostar IRAS 4A, which is undergoing collapse in the active molecular cloud NGC 1333, to set constraints on this process. In particular we want to measure the mass infall rate and investigate whether the collapse could have been triggered by a strong external perturbation. We analyze existing continuum observations to derive the density structure of the envelope, and use our new molecular line observations done with the IRAM 30m telescope to probe its velocity structure. We perform a detailed comparison of this set of data with a numerical model of collapse triggered by a fast external compression. Both the density and velocity structures of the envelope can be well fitted by this model of collapse induced by a fast external compression for a time elapsed since point mass formation of 1-2 x 10**4 yr. We deduce a large mass infall rate of 0.7-2 x 10**-4 Msun/yr. The momentum required for the perturbation to produce this large mass infall rate is of the same order as the momenta measured for the NGC 1333 numerous outflows. Our analysis shows also that the turbulence is highly non uniform in the envelope, dropping from supersonic to subsonic values toward the center. The inner subsonic turbulence is most likely a relic of the conditions prevailing in the dense core before the onset of collapse. The vigorous collapse undergone by IRAS 4A was triggered by a fast external compression, probably related to the expansion of a nearby cavity, which could have triggered the collapse of the nearby Class 0 protostar IRAS 4B simultaneously. This cavity could have been generated by an outflow but we have not found a good protostellar candidate yet.Comment: Accepted by A&A. 12 pages, 10 figure

Apparent Metallic Behavior at B = 0 of a two-dimensional electron system in AlAs

We report the observation of metallic-like behavior at low temperatures and zero magnetic field in two dimensional (2D) electrons in an AlAs quantum well. At high densities the resistance of the sample decreases with decreasing temperature, but as the density is reduced the behavior changes to insulating, with the resistance increasing as the temperature is decreased. The effect is similar to that observed in 2D electrons in Si-MOSFETs, and in 2D holes in SiGe and GaAs, and points to the generality of this phenomenon