Quality of milk in Manchego sheep flocks. A review

Esta obra está bajo una licencia de Creative Commons Reconocimiento-CompartirIgual 4.0 Internacional.-- et al.[EN]: Improvement of milk quality is one of the principal objectives in dairy sheep production systems. Regulator Council of PDO Manchego Cheese and National Association of Manchega Breeders take part in some milk quality research projects from Dairy Laboratory of the Regional Selection and Animal Reproduction Center, belonging to the Regional Government of Castilla-La Mancha, has developed. The study aims are to find the relationship between the management characteristics of the farms and the quality of bulk tank sheep milk (in differential features of composition, microbiology and technological components). Knowledge of these relationships informs about the main aspects to consider (preservation of milk, feeding systems of sheep, associations, etc.) in sheep milk production for cheese making.[ES]: La mejora de la calidad de la leche es uno de los objetivos fundamentales en los actuales sistemas de producción del ovino lechero. La Fundación Consejo Regulador de la Denominación de Origen Queso Manchego y la Asociación Nacional de Criadores de Ganado Ovino Selecto raza Manchega participan en los Proyectos de Investigación en materia de calidad de la leche desarrollados por el Laboratorio de Lactología del Centro Regional de Selección y Reproducción Animal de la Junta de Comunidades de Castilla-La Mancha. Estos trabajos tienen como objetivo principal el estudio de las relaciones entre las características productivas de las ganaderías y la calidad integral de la leche de oveja (composición, microbiología diferencial y componentes tecnológicos). Los resultados de estos trabajos incluyen el análisis de factores tales como la conservación de la leche, la alimentación de las ovejas de ordeño, el nivel de asociacionismo, etc, en la producción de leche de oveja destinada a la elaboración de queso.Peer Reviewe

Spatiotemporal changes in aerosol properties by hygroscopic growth and impacts on radiative forcing and heating rates during DISCOVER-AQ 2011

This research has been supported by the H2020 Marie Sklodowska-Curie Actions (grant no. 778349), the Spanish Ministry of Economy and Competitiveness (RTI2018101154.A.I00), and by the Russian Science Foundation (project 2117-00114, entitled Development of lidar retrieval algorithms).This work focuses on the characterization of vertically resolved aerosol hygroscopicity properties and their direct radiative effects through a unique combination of ground-based and airborne remote sensing measurements during the Column and Vertically Resolved Observations Relevant to Air Quality (DISCOVER-AQ) 2011 field campaign in the Baltimore-Washington DC metropolitan area. To that end, we combined aerosol measurements from a multiwavelength Raman lidar located at NASA Goddard Space Flight Center and the airborne NASA Langley High Spectral Resolution Lidar-1 (HSRL-1) lidar system. In situ measurements aboard the P-3B airplane and ground-based Aerosol Robotic Network - Distributed Regional Aerosol Gridded Observational Network (AERONET-DRAGON) served to validate and complement quantifications of aerosol hygroscopicity from lidar measurements and also to extend the study both temporally and spatially. The focus here is on 22 and 29 July 2011, which were very humid days and characterized by a stable atmosphere and increasing relative humidity with height in the planetary boundary layer (PBL). Combined lidar and radiosonde (temperature and water vapor mixing ratio) measurements allowed the retrieval of the Hanel hygroscopic growth factor which agreed with that obtained from airborne in situ measurements and also explained the significant increase of extinction and backscattering with height. Airborne measurements also confirmed aerosol hygroscopicity throughout the entire day in the PBL and identified sulfates and water-soluble organic carbon as the main species of aerosol particles. The combined Raman and HSRL-1 measurements permitted the inversion for aerosol microphysical properties revealing an increase of particle radius with altitude consistent with hygroscopic growth. Aerosol hygroscopicity pattern served as a possible explanation of aerosol optical depth increases during the day, particularly for fine-mode particles. Lidar measurements were used as input to the libRadtran radiative transfer code to obtain vertically resolved aerosol radiative effects and heating rates under dry and humid conditions, and the results reveal that aerosol hygroscopicity is responsible for larger cooling effects in the shortwave range (7-10 W m(-2) depending on aerosol load) near the ground, while heating rates produced a warming of 0.12 K d(-1) near the top of PBL where aerosol hygroscopic growth was highest.H2020 Marie Sklodowska-Curie Actions 778349Spanish Ministry of Economy and Competitiveness RTI2018101154.A.I00Russian Science Foundation (RSF) 2117-0011

Use of resistivity measurements to detect urban caves in Mexico City and to assess the related hazard

International audienceIn the XIX century when Mexico City was much smaller than at present, there was non-regulated mining of building materials in a region of tuffs northwest of the city in an inhabited countryside. With the growth of the city during the XX century, this region was increasingly populated and in the 1970's many two-level bricks houses were built, without regard for underground caves created by the earlier extractions. Some ground sinkings in adjacent areas alarmed the residents who now are worried about this permanent hazard. An association of residents contracted a private company for a geophysical study in order to know the distribution of the caves. Resistivity measurements were taken in the area to detect the caves in order to alert city authorities. Resistivity data along most of the streets were collected with the array pole-dipole that consisted of three grounded electrodes. We performed 2-D dimensional inversions to the data in order to get a 2-D resistivity image of every street. This is similar to a resistivity cross-section of the ground but obtained from the inversion of pole-dipole and Schlumberger resistivity data simultaneously. Using the information of previous drills we modified our programming code in order to perform constrained inversion and to get more accurate resistivity models in agreement with the drills. From the resistivity models obtained for every street it was possible to produce a map which shows the horizontal distribution of the resistive bodies at a depth of 12m. These resistive bodies show coherent alignments that seem to correspond with a distributions of interconnected caves or tunnels used for extracting the sandy-tuffs. From these kind of interpretation method it was intended to get a more accurate horizontal distribution of the excavated areas in order to better know the urbanized area affected and lead the authorities to remedy the area with refill material

Effective interactions of colloids on nematic films

The elastic and capillary interactions between a pair of colloidal particles trapped on top of a nematic film are studied theoretically for large separations $d$. The elastic interaction is repulsive and of quadrupolar type, varying as $d^{-5}$. For macroscopically thick films, the capillary interaction is likewise repulsive and proportional to $d^{-5}$ as a consequence of mechanical isolation of the system comprised of the colloids and the interface. A finite film thickness introduces a nonvanishing force on the system (exerted by the substrate supporting the film) leading to logarithmically varying capillary attractions. However, their strength turns out to be too small to be of importance for the recently observed pattern formation of colloidal droplets on nematic films.Comment: 13 pages, accepted by EPJ

Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections

SummaryObjectivesThe kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue infection were studied.DesignSerum, saliva, and urine samples were collected from 22 patients with clinical and confirmed dengue 3 virus infection during the outbreak in Havana City in 2001. They were tested by capture IgM (MAC-ELISA), IgA (AAC-ELISA), and IgE (EAC-ELISA) and IgG ELISA inhibition method (EIM) to detect specific dengue antibodies.ResultsSimilar kinetics were observed in IgM, IgA, and IgG antibodies in saliva and IgA and IgG in urine samples from secondary cases compared with kinetics in serum samples, although the values were lower. No IgG antibody was detected in saliva and urine samples in primary cases and IgM antibody was not detected in urine samples from either primary or secondary infection. All secondary cases were positive for IgG in saliva and urine samples at day 7. The kinetics of specific IgE antibodies in primary and secondary cases were different.ConclusionsThe kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infection were studied for the first time, showing its behavior and usefulness in dengue virus diagnosis. The specific IgE could play a role as a serological marker in secondary infections

Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega rams

The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37°C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which spermatozoa will be subject to in the ewe uterus. The effects of temperature and temperature-humidity index (THI) from day 60 prior collection to the date of semen collection on DFI were examined. To better understand the causes determining the sensitivity of spermatozoa to heat, this study was conducted in 60 males with alternative genotypes for the SNP G/C-660 of the HSP90AA1 promoter, which encode for the Hsp90α protein. The Hsp90α protein predominates in the brain and testis, and its role in spermatogenesis has been described in several species. Ridge regression analyses showed that days 29 to 35 and 7 to 14 before sperm collection (bsc) were the most critical regarding the effect of heat stress over DFI values. Mixed model analyses revealed that DFI increases over a threshold of 30°C for maximum temperature and 22 for THI at days 29 to 35 and 7 to 14 bsc only in animals carrying the GG-660 genotype. The period 29-35 bsc coincide with the meiosis I process for which the effect of the Hsp90α has been described in mice. The period 7-14 bsc may correspond with later stages of the meiosis II and early stages of epididymal maturation in which the replacement of histones by protamines occurs. Because of GG-660 genotype has been associated to lower levels of HSP90AA1 expression, suboptimal amounts of HSP90AA1 mRNA in GG-660 animals under heat stress conditions make spermatozoa DNA more susceptible to be fragmented. Thus, selecting against the GG-660 genotype could decrease the DNA fragmentation and spermatozoa thermal susceptibility in the heat season, and its putative subsequent fertility gains.This work was supported by RTA2009-00098-00-00 INIA project (Subprograma de Investigación Fundamental orientada a los Recursos y Tecnologías Agrarias).Peer Reviewe

Caracterización tecnofuncional de harinas obtenidas de la cascara y semillas de pepino (Cucumis sativus) y su aplicación en un alimento funcional.

En la actualidad surge la idea de utilizar las frutas en forma de harina como una alternativa de ingrediente funcional. El objetivo del presente trabajo fue caracterizar las propiedades bromatológicas y funcionales harinas de cascara y semillas de pepino obtenidas como subproducto. En general se observan diferencias estadísticamente significativas entre ambas harinas de cascara y semillas obtenidas a partir de pepino, destacando el contenido proteico de 16.24 ± 0.320 y 20.42 ± 0.244% respectivamente y de fibra 17.29±0.990 y 27.91±0.088% respectivamente de ambas harinas por ende podrían utilizarse como ingrediente funcional en la realización de distintos alimentos, se realizaron galletas horneadas a partir de la harina de cascara de pepino debido al contenido de humedad de esta, y se obtuvieron características organolépticas aceptables

LRP10, PGK1 and RPLP0: best reference genes in periprostatic adipose tissue under obesity and prostate cancer conditions

Obesity (OB) is a metabolic disorder characterized by adipose tissue dysfunction that has emerged as a health problem of epidemic proportions in recent decades. OB is associated with multiple comorbidities, including some types of cancers. Specifically, prostate cancer (PCa) has been postulated as one of the tumors that could have a causal relationship with OB. Particularly, a specialized adipose tissue (AT) depot known as periprostatic adipose tissue (PPAT) has gained increasing attention over the last few years as it could be a key player in the pathophysiological interaction between PCa and OB. However, to date, no studies have defined the most appropriate internal reference genes (IRGs) to be used in gene expression studies in this AT depot. In this work, two independent cohorts of PPAT samples (n = 20/n = 48) were used to assess the validity of a battery of 15 literature-selected IRGs using two widely used techniques (reverse transcription quantitative PCR [RT-qPCR] and microfluidic-based qPCR array). For this purpose, ΔCt method, GeNorm (v3.5), BestKeeper (v1.0), NormFinder (v.20.0), and RefFinder software were employed to assess the overall trends of our analyses. LRP10, PGK1, and RPLP0 were identified as the best IRGs to be used for gene expression studies in human PPATs, specifically when considering PCa and OB conditions

On the nature of the extragalactic number counts in the K-band

We investigate the causes of the different shape of the $K$-band number counts when compared to other bands, analyzing in detail the presence of a change in the slope around $K\sim17.5$. We present a near-infrared imaging survey, conducted at the 3.5m telescope of the Calar Alto Spanish-German Astronomical Center (CAHA), covering two separated fields centered on the HFDN and the Groth field, with a total combined area of $\sim0.27$deg$^{2}$ to a depth of $K\sim19$ ($3\sigma$,Vega). We derive luminosity functions from the observed $K$-band in the redshift range [0.25-1.25], that are combined with data from the references in multiple bands and redshifts, to build up the $K$-band number count distribution. We find that the overall shape of the number counts can be grouped into three regimes: the classic Euclidean slope regime ($d\log N/dm\sim0.6$) at bright magnitudes; a transition regime at intermediate magnitudes, dominated by $M^{\ast}$ galaxies at the redshift that maximizes the product $\phi^{\ast}\frac{dV_{c}}{d\Omega}$; and an $\alpha$ dominated regime at faint magnitudes, where the slope asymptotically approaches -0.4($\alpha$+1) controlled by post-$M^{\ast}$ galaxies. The slope of the $K$-band number counts presents an averaged decrement of $\sim50$ in the range $15.5<K<18.5$ ($d\log N/dm\sim0.6-0.30$). The rate of change in the slope is highly sensitive to cosmic variance effects. The decreasing trend is the consequence of a prominent decrease of the characteristic density $\phi^{\ast}_{K,obs}$ ($\sim60$ from $z=0.5$ to $z=1.5$) and an almost flat evolution of $M^{\ast}_{K,obs}$ (1$\sigma$ compatible with $M^{\ast}_{K,obs}=-22.89\pm0.25$ in the same redshift range).Comment: 18 pages, 22 figures, Accepted by Astronomy & Astrophysic

FeCo Nanowire-Strontium Ferrite Powder Composites for Permanent Magnets with High-Energy Products

Due to the issues associated with rare-earth elements, there arises a strong need for magnets with properties between those of ferrites and rare-earth magnets that could substitute the latter in selected applications. Here, we produce a high remanent magnetization composite bonded magnet by mixing FeCo nanowire powders with hexaferrite particles. In the first step, metallic nanowires with diameters between 30 and 100 nm and length of at least 2 {\mu}m are fabricated by electrodeposition. The oriented as-synthesized nanowires show remanence ratios above 0.76 and coercivities above 199 kA/m and resist core oxidation up to 300 {\deg}C due to the existence of a > 8 nm thin oxide passivating shell. In the second step, a composite powder is fabricated by mixing the nanowires with hexaferrite particles. After the optimal nanowire diameter and composite composition are selected, a bonded magnet is produced. The resulting magnet presents a 20% increase in remanence and an enhancement of the energy product of 48% with respect to a pure hexaferrite (strontium ferrite) magnet. These results put nanowire-ferrite composites at the forefront as candidate materials for alternative magnets for substitution of rare earths in applications that operate with moderate magnet performance