1,422 research outputs found
The Information Content of the 1999 Announcement of Funds from Operations (FFO) Changes for Real Estate Investment Trusts
This study examines the market response to the 1999 announcement of a change in accounting for Funds from Operations (FFO) for Real Estate Investment Trusts (REITs). This change provides an increase in transparency in the accounting statements of REITs regarding the calculation of FFO. An analysis of this announcement finds that shareholder wealth increases but the significance of that increase is questionable. Additionally, an analysis of the adverse selection component of the bid-ask spread finds weak evidence to support the conjecture that the amount of information asymmetry in REIT prices declines after the announcement of the FFO accounting change.
Failure Analysis Study and Long-Term Reliability of Optical Assemblies with End-Face Damage
In June 2005, the NESC received a multi-faceted request to determine the long term reliability of fiber optic termini on the ISS that exhibited flaws not manufactured to best workmanship practices. There was a lack of data related to fiber optic workmanship as it affects the long term reliability of optical fiber assemblies in a harsh environment. A fiber optic defect analysis was requested which would find and/or create various types of chips, spalls, scratches, etc., that were identified by the ISS personnel. Once the defects and causes were identified the next step would be to perform long term reliability testing of similar assemblies with similar defects. The goal of the defect analysis would be for the defects to be observed and documented for deterioration of fiber optic performance. Though this report mostly discusses what has been determined as evidence of poor manufacturing processes, it also concludes the majority of the damage could have been avoided with a rigorous process in place
Coherence length of an elongated condensate: a study by matter-wave interferometry
We measure the spatial correlation function of Bose-Einstein condensates in
the cross-over region between phase-coherent and strongly phase-fluctuating
condensates. We observe the continuous path from a gaussian-like shape to an
exponential-like shape characteristic of one-dimensional phase-fluctuations.
The width of the spatial correlation function as a function of the temperature
shows that the condensate coherence length undergoes no sharp transition
between these two regimes.Comment: 8 pages, 6 figure, submitted to EPJ
Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
Three-dimensional models of human intestinal epithelium mimic the differentiated form and function of parental tissues often not exhibited by two-dimensional monolayers and respond to Salmonella in key ways that reflect in vivo infections. To further enhance the physiological relevance of three-dimensional models to more closely approximate in vivo intestinal microenvironments encountered by Salmonella, we developed and validated a novel three-dimensional co-culture infection model of colonic epithelial cells and macrophages using the NASA Rotating Wall Vessel bioreactor. First, U937 cells were activated upon collagen-coated scaffolds. HT-29 epithelial cells were then added and the three-dimensional model was cultured in the bioreactor until optimal differentiation was reached, as assessed by immunohistochemical profiling and bead uptake assays. The new co-culture model exhibited in vivo-like structural and phenotypic characteristics, including three-dimensional architecture, apical-basolateral polarity, well-formed tight/adherens junctions, mucin, multiple epithelial cell types, and functional macrophages. Phagocytic activity of macrophages was confirmed by uptake of inert, bacteria-sized beads. Contribution of macrophages to infection was assessed by colonization studies of Salmonella pathovars with different host adaptations and disease phenotypes (Typhimurium ST19 strain SL1344 and ST313 strain D23580; Typhi Ty2). In addition, Salmonella were cultured aerobically or microaerobically, recapitulating environments encountered prior to and during intestinal infection, respectively. All Salmonella strains exhibited decreased colonization in co-culture (HT-29-U937) relative to epithelial (HT-29) models, indicating antimicrobial function of macrophages. Interestingly, D23580 exhibited enhanced replication/survival in both models following invasion. Pathovar-specific differences in colonization and intracellular co-localization patterns were observed. These findings emphasize the power of incorporating a series of related three-dimensional models within a study to identify microenvironmental factors important for regulating infection
Generation of unpredictable time series by a Neural Network
A perceptron that learns the opposite of its own output is used to generate a
time series. We analyse properties of the weight vector and the generated
sequence, like the cycle length and the probability distribution of generated
sequences. A remarkable suppression of the autocorrelation function is
explained, and connections to the Bernasconi model are discussed. If a
continuous transfer function is used, the system displays chaotic and
intermittent behaviour, with the product of the learning rate and amplification
as a control parameter.Comment: 11 pages, 14 figures; slightly expanded and clarified, mistakes
corrected; accepted for publication in PR
High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Leaf senescence is an essential developmental process that impacts dramatically on crop yields and involves altered
regulation of thousands of genes and many metabolic and signaling pathways, resulting in major changes in the leaf. The
regulation of senescence is complex, and although senescence regulatory genes have been characterized, there is little
information on how these function in the global control of the process. We used microarray analysis to obtain a highresolution
time-course profile of gene expression during development of a single leaf over a 3-week period to senescence.
A complex experimental design approach and a combination of methods were used to extract high-quality replicated data
and to identify differentially expressed genes. The multiple time points enable the use of highly informative clustering to
reveal distinct time points at which signaling and metabolic pathways change. Analysis of motif enrichment, as well
as comparison of transcription factor (TF) families showing altered expression over the time course, identify clear groups
of TFs active at different stages of leaf development and senescence. These data enable connection of metabolic
processes, signaling pathways, and specific TF activity, which will underpin the development of network models to
elucidate the process of senescence
Suppression of Aβ toxicity by puromycin-sensitive aminopeptidase is independent of its proteolytic activity.
The accumulation of β-amyloid (Aβ) peptide in the brain is one of the pathological hallmarks of Alzheimer's disease and is thought to be of primary aetiological significance. In an unbiased genetic screen, we identified puromycin-sensitive aminopeptidase (PSA) as a potent suppressor of Aβ toxicity in a Drosophila model system. We established that coexpression of Drosophila PSA (dPSA) in the flies' brains improved their lifespan, protected against locomotor deficits, and reduced brain Aβ levels by clearing the Aβ plaque-like deposits. However, confocal microscopy and subcellular fractionation of amyloid-expressing 7PA2 cells demonstrated that PSA localizes to the cytoplasm. Therefore, PSA and Aβ are unlikely to be in the same cellular compartment; moreover, when we artificially placed them in the same compartment in flies, we could not detect a direct epistatic interaction. The consequent hypothesis that PSA's suppression of Aβ toxicity is indirect was supported by the finding that Aβ is not a proteolytic substrate for PSA in vitro. Furthermore, we showed that the enzymatic activity of PSA is not required for rescuing Aβ toxicity in neuronal SH-SY5Y cells. We investigated whether the stimulation of autophagy by PSA was responsible for these protective effects. However PSA's promotion of autophagosome fusion with lysosomes required proteolytic activity and so its effect on autophagy is not identical to its protection against Aβ toxicity
The characteristics of millisecond pulsar emission: I. Spectra, pulse shapes and the beaming fraction
We have monitored a large sample of millisecond pulsars using the 100-m
Effelsberg radio telescope in order to compare their radio emission properties
to the slowly rotating population. With some notable exceptions, our findings
suggest that the two groups of objects share many common properties. A
comparison of the spectral indices between samples of normal and millisecond
pulsars demonstrates that millisecond pulsar spectra are not significantly
different from those of normal pulsars. There is evidence, however, that
millisecond pulsars are slightly less luminous and less efficient radio
emitters compared to normal pulsars. We confirm recent suggestions that a
diversity exists among the luminosities of millisecond pulsars with the
isolated millisecond pulsars being less luminous than the binary millisecond
pulsars. There are indications that old millisecond pulsars exhibit somewhat
flatter spectra than the presumably younger ones. We present evidence that
millisecond pulsar profiles are only marginally more complex than those found
among the normal pulsar population. Moreover, the development of the profiles
with frequency is rather slow, suggesting very compact magnetospheres. The
profile development seems to anti-correlate with the companion mass and the
spin period, again suggesting that the amount of mass transfer in a binary
system might directly influence the emission properties. The angular radius of
radio beams of millisecond pulsars does not follow the scaling predicted from a
canonical pulsar model which is applicable for normal pulsars. Instead they are
systematically smaller. The smaller inferred luminosity and narrower emission
beams will need to be considered in future calculations of the birth-rate of
the Galactic population.Comment: 40 pages, 14 figures, accepted for publication in Ap
High sensitivity measurements of the CMB power spectrum with the extended Very Small Array
We present deep Ka-band ( GHz) observations of the CMB made
with the extended Very Small Array (VSA). This configuration produces a
naturally weighted synthesized FWHM beamwidth of arcmin which covers
an -range of 300 to 1500. On these scales, foreground extragalactic
sources can be a major source of contamination to the CMB anisotropy. This
problem has been alleviated by identifying sources at 15 GHz with the Ryle
Telescope and then monitoring these sources at 33 GHz using a single baseline
interferometer co-located with the VSA. Sources with flux densities \gtsim 20
mJy at 33 GHz are subtracted from the data. In addition, we calculate a
statistical correction for the small residual contribution from weaker sources
that are below the detection limit of the survey.
The CMB power spectrum corrected for Galactic foregrounds and extragalactic
point sources is presented. A total -range of 150-1500 is achieved by
combining the complete extended array data with earlier VSA data in a compact
configuration. Our resolution of allows the first 3
acoustic peaks to be clearly delineated. The is achieved by using mosaiced
observations in 7 regions covering a total area of 82 sq. degrees. There is
good agreement with WMAP data up to where WMAP data run out of
resolution. For higher -values out to , the agreement in
power spectrum amplitudes with other experiments is also very good despite
differences in frequency and observing technique.Comment: 16 pages. Accepted in MNRAS (minor revisions
Radio source calibration for the VSA and other CMB instruments at around 30 GHz
Accurate calibration of data is essential for the current generation of CMB
experiments. Using data from the Very Small Array (VSA), we describe procedures
which will lead to an accuracy of 1 percent or better for experiments such as
the VSA and CBI. Particular attention is paid to the stability of the receiver
systems, the quality of the site and frequent observations of reference
sources. At 30 GHz the careful correction for atmospheric emission and
absorption is shown to be essential for achieving 1 percent precision. The
sources for which a 1 percent relative flux density calibration was achieved
included Cas A, Cyg A, Tau A and NGC7027 and the planets Venus, Jupiter and
Saturn. A flux density, or brightness temperature in the case of the planets,
was derived at 33 GHz relative to Jupiter which was adopted as the fundamental
calibrator. A spectral index at ~30 GHz is given for each. Cas A,Tau A, NGC7027
and Venus were examined for variability. Cas A was found to be decreasing at
percent per year over the period March 2001 to August 2004.
In the same period Tau A was decreasing at percent per year. A
survey of the published data showed that the planetary nebula NGC7027 decreased
at percent per year over the period 1967 to 2003. Venus showed
an insignificant ( percent) variation with Venusian illumination.
The integrated polarization of Tau A at 33 GHz was found to be
percent at pa .}Comment: 13 pages, 15 figures, submitted to MNRA
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