Toward Forecasting Volcanic Eruptions using Seismic Noise

During inter-eruption periods, magma pressurization yields subtle changes of the elastic properties of volcanic edifices. We use the reproducibility properties of the ambient seismic noise recorded on the Piton de la Fournaise volcano to measure relative seismic velocity variations of less than 0.1 % with a temporal resolution of one day. Our results show that five studied volcanic eruptions were preceded by clearly detectable seismic velocity decreases within the zone of magma injection. These precursors reflect the edifice dilatation induced by magma pressurization and can be useful indicators to improve the forecasting of volcanic eruptions.Comment: Supplementary information: http://www-lgit.obs.ujf-grenoble.fr/~fbrengui/brenguier_SI.pdf Supplementary video: http://www-lgit.obs.ujf-grenoble.fr/~fbrengui/brenguierMovieVolcano.av

Harvesting Electricity with Geobacter bremensis Isolated from Compost

Electrochemically active (EA) biofilms were formed on metallic dimensionally stable anode-type electrode (DSA), embedded in garden compost and polarized at +0.50 V/SCE. Analysis of 16S rRNA gene libraries revealed that biofilms were heavily enriched in Deltaproteobacteria in comparison to control biofilms formed on non-polarized electrodes, which were preferentially composed of Gammaproteobacteria and Firmicutes. Among Deltaproteobacteria, sequences affiliated with Pelobacter and Geobacter genera were identified. A bacterial consortium was cultivated, in which 25 isolates were identified as Geobacter bremensis. Pure cultures of 4 different G. bremensis isolates gave higher current densities (1400 mA/m2 on DSA, 2490 mA/m2 on graphite) than the original multi-species biofilms (in average 300 mA/m2 on DSA) and the G. bremensis DSM type strain (100–300 A/m2 on DSA; 2485 mA/m2 on graphite). FISH analysis confirmed that G. bremensis represented a minor fraction in the original EA biofilm, in which species related to Pelobacter genus were predominant. The Pelobacter type strain did not show EA capacity, which can explain the lower performance of the multi-species biofilms. These results stressed the great interest of extracting and culturing pure EA strains from wild EA biofilms to improve the current density provided by microbial anodes

Molecules in bipolar proto-planetary nebulae

Two bipolar proto-planetary nebulae, IRAS16594-4656 and IRAS17150-3224, have been detected in various molecular lines, namely CO, ^{13}CO, HCN and CN, and remain undetected in several other species. CO(J=2-1) and CO(J=3-2) line profiles are compared to new spectra of similar PPN candidates, previously undetected in CO(J=2-1): CPD-53^{o}5736, IRAS17106-3046, IRAS17245-3951 and IRAS17441-2411. CO(J=2-1) maps of IRAS16594-4656 and IRAS17150-3224 show that both PPNe have little separation between blue, centre and red-shifted emission, and also that the CO(J=2-1) emission is of a similar size to the telescope beam. Fractional abundances of all detected molecules (except CO) are calculated using the results of CO line modelling and a simple photodissociation model. For those species not detected, upper limits are derived. Comparisons between these fractional abundances and those of other PPNe show that IRAS16594-4656 and IRAS17150-3224 are quite under-abundant when compared to molecule-rich sources like CRL618, CRL2688 and OH231.8+4.2. As a reason for this deficit, the difference in circumstellar envelope/torus density between the molecule-rich sources and the molecule-poor sources is proposed, and supported by a chemical model which follows the transition of a circumstellar envelope through the AGB phase and into the PPN phase of evolution. The model includes the effects of UV radiation, cosmic rays and also X-rays. Finally, the post-AGB ages of these two objects (200-400 yr) are estimated using CN/HCN and HCN/CO ratios and both ages are found to be in agreement with previous figures cited in the literature, IRAS17150-3224 being the younger of the two PPNe.Comment: Accepted by Astronomy & Astrophysics. 21 pages, 11 figure

Fishing for biodiversity: Novel methanopterin-linked C1 transfer genes deduced from the Sargasso Sea metagenome

The recently generated database of microbial genes from an oligotrophic environment populated by a calculated 1,800 of major phylotypes (the Sargasso Sea metagenome) presents a great source for expanding local databases of genes indicative of a specific function. In this paper we analyze the Sargasso Sea metagenome in terms of the presence of methanopterin-linked C1 transfer genes that are signature for methylotrophy. We conclude that more than 10 phylotypes possessing genes of interest are present in this environment, and a few of these are relatively abundant species. The sequences representative of the major phylotypes do not appear to belong to any known microbial group capable of methanopterin-linked C1 transfer. Instead, they separate from all known sequences on phylogenetic trees, pointing towards their affiliation with a novel microbial phylum. These data imply a broader distribution of methanopterin-linked functions in the microbial world than previously known

Analysis of fae and fhcD Genes in Mono Lake, California

Genes for two enzymes of the tetrahydromethanopterin-linked C(1) transfer pathway (fae and fhcD) were detected in hypersaline, hyperalkaline Mono Lake (California), via PCR amplification and analysis. Low diversity for fae and fhcD was noted, in contrast to the diversity previously detected in a freshwater lake, Lake Washington (Washington)

Highly Divergent Genes for Methanopterin-Linked C(1) Transfer Reactions in Lake Washington, Assessed via Metagenomic Analysis and mRNA Detection

The origins and the evolutionary history of tetrahydromethanopterin-linked C(1) transfer reactions that are part of two environmentally important biotransformations, methylotrophy and methanogenesis, are still not well understood. In previous studies, we have expanded the known phylogenetic diversity of these reactions by identifying genes highly diverging from the ones associated with cultivated Proteobacteria, Planctomycetes, or Archaea (M. G. Kalyuzhnaya, M. E. Lidstrom, and L. Chistoserdova, Microb. Ecol. 48:463-472, 2004; M. G. Kalyuzhnaya, O. Nercessian, M. E. Lidstrom, and L. Chistoserdova, Environ. Microbiol. 7:1269-1274, 2005). Here we used a metagenomic approach to demonstrate that these divergent genes are present with high abundance in the microbial community inhabiting Lake Washington sediment. We also gained preliminary insights into the genomic composition of the organisms possessing these genes by sequencing genomic fragments from three uncultured microbes possessing the genes of interest. Phylogenetic analyses suggested that, although distantly related to each other, these organisms deeply diverge from known Bacteria and Archaea, with more relation to the former, suggesting their affiliation with a new bacterial phylum. We also demonstrate, via specific mRNA detection, that these divergent genes are expressed in the environment, pointing toward their potential role in local carbon cycling

Bacterial Populations Active in Metabolism of C(1) Compounds in the Sediment of Lake Washington, a Freshwater Lake

Active members of the bacterial community in the sediment of Lake Washington, with special emphasis on C(1) utilizers, were identified by employing two complementary culture-independent approaches: reverse transcription of environmental mRNA and 16S rRNA combined with PCR (RT-PCR) and stable-isotope probing (SIP) of DNA with the (13)C-labeled C(1) substrates methanol, methylamine, formaldehyde, and formate. Analysis of RT-PCR-amplified fragments of 16S rRNA-encoding genes revealed that gammaproteobacterial methanotrophs belonging to Methylobacter and Methylomonas dominate the active methylotroph population, while only one other known methylotrophic lineage, Methylophilaceae, was detected via this approach. Analysis of RT-PCR-amplified functional genes, pmoA and fae, allowed detection of alphaproteobacterial (Methylosinus) and gammaproteobacterial (Methylobacter, Methylomonas, and Methylomicrobium) methanotrophs, methylotrophs of the genus Methylobacterium, and yet-unidentified proteobacteria. SIP experiments allowed detection of a broad variety of groups actively metabolizing C(1) compounds. Comparisons between 16S rRNA gene pools amplified from [(13)C]DNA and from [(12)C]DNA revealed that the proportion of Methylophilus-related sequences increased in the presence of [(13)C]methanol, [(13)C]methylamine, and [(13)C]formaldehyde; Novosphingobium-related sequences were enriched in the presence of [(13)C]methanol; Gemmatimonadaceae-related sequences were enriched in the presence of [(13)C]formaldehyde and [(13)C]formate; and Xanthomonadaceae-related sequences were enriched in the presence of [(13)C]formate. Analysis of fae genes amplified from [(13)C]DNAs isolated from different microcosms revealed specific shifts in populations in response to a specific C(1) compound: Methylosinus sequences dominated the [(13)C]methanol microcosm pool, and beta- and gammaproteobacterial sequences dominated the [(13)C]methylamine microcosm pool. The [(13)C]formaldehyde microcosm was dominated by betaproteobacterial sequences and by sequences of a nonaffiliated group, while the [(13)C]formate microcosm was dominated by alpha- and betaproteobacterial sequences. Overall, these data point toward the presence of a diverse population of active methylotrophs in Lake Washington sediments and toward the existence of yet-uncultivated organisms

Localisation and electrochemical activity of <i>G. bremensis</i> isolates.

<p>Chronoamperometry (A) and FISH (B) performed with <i>G. bremensis</i> isolate ONC105, isolate ONC102, the type strain DSM 12179 and control experiment (not inoculated). A: Current density in 4 independent reactors with DSA electrodes polarized at 0.50 V vs Ag/AgCl as electron acceptor and ethanol (10 mM) as electron donor. B: FISH analysis of DSA electrodes by using 16 S rRNA probes directed specificaly against <i>Geobacter</i> species.</p