Programa de estabilidad emocional para el bienestar social en los familiares con duelo por Covid-19

La presente investigación tuvo como principal objetivo aplicar un programa de estabilidad emocional para el bienestar social en los familiares con duelo por COVID-19 de los pacientes del C.S.J. Olaya, Chiclayo. Para ello, se indagaron las causas que originaron el problema, basadas en que la insuficiencia en el proceso de estabilidad emocional limita el bienestar social. Por otro lado, la investigación se desarrolló mediante un enfoque científico mixto, tipo aplicada, nivel explicativo y diseño cuasi experimental; en la cual, la muestra estuvo sujeta a 118 familiares de pacientes fallecidos por COVID-19, y se trianguló (método DITRIAC) con 5 enfermeros, 5 médicos y 5 psicólogas, a quienes se les aplicó los instrumentos de recolección de datos con el propósito de diagnosticar el estado actual del bienestar social del grupo control y experimental; luego, para el post test solo se recopiló información de 10 pacientes, del grupo control y experimental respectivamente, todo ello permitió obtener una perspectiva integral del estudio. Como resultados iniciales se tuvo que la estabilidad emocional de los pacientes del C.S.J. Olaya influye de manera directa y significativa (p=0.00; rho Spearman=0.527) en el bienestar social de los mismos, la cual desencadenó que el nivel de bienestar social de aquellos pacientes fuera categorizado como bajo (80%), asociados a problemas de cohesión (92%), autocontrol (90%), influencia (81%) y valoración (32%). Finalmente, se llegó a concluir que el programa de estabilidad emocional fue efectivo para tratar el bienestar social de los familiares en duelo por COVID-19, encontrando diferencias estadísticamente significativas entre los puntajes del post test del grupo control y experimental; en este sentido, los pacientes que recibieron el tratamiento mejoraron notablemente sus capacidades para la solución de problemas en la sociedad, y los problemas de intervención psicosocial que les aquejaban se redujeron considerablemente.TesisCiencias de la vida y cuidado de la salud human

Catalytic transformation of biomass-derived 5-hydroxymethylfurfural over supported bimetallic iridium-based catalysts

5-Hydroxymethylfurfural (HMF) is a biobased platform chemical that can be valorized into a spectrum of valuable products. In this report, supported Ir, Ir–Co, Ir–Ni, and Ir–Ru catalysts were investigated for this purpose. Only hydrogenation of HMF to 2,5-bis-(hydroxymethyl)furan (BHMF) occurred over all catalysts. The effect of the second metal (Co, Ni, and Ru) on Ir/SiO2 was reflected by the kinetic constants being in the order Ir–Ni/SiO2 > Ir–Co/SiO2 > Ir–Ru/SiO2. The oxophilic nature of the secondary metal improved the catalytic performance of the bimetallic catalysts compared to the monometallic iridium catalyst (Ir/SiO2). Addition of HCOOH and H2SO4 as cocatalysts is a strategy to reach one-pot conversion of HMF to 2,5-di-methylfuran (DMF). Over-hydrogenolysis products such as 2,5-dimethyltetrahydrofuran were formed when only H2SO4 was added, giving higher activity compared to addition of HCOOH. Simultaneous presence of acids gave the highest HMF conversion, promoting esterification to 5-formyloxymethyl furfural and allowing the one-pot transformation of HMF to DMF. Thermodynamic analysis of HMF transformations revealed that both hydrogenation and dehydration steps are feasible.Peer ReviewedPostprint (author's final draft

Post-Marketing Health Technology Monitoring. The Analysis of an Experience from a Clinical Perspective

Introduction: A system for monitoring the use of aphaeresis in the treatment of ulcerative colitis (UC), named system for monitoring aphaeresis in ulcerative colitis (SiMAC), was designed in 2006 in the Basque Country. In the present study, the opinion of the clinicians who participated in SiMAC was evaluated, in order to identify the barriers and gather suggestions that could improve implementation of this kind of system. Methods: A mixed questionnaire was designed, in order to gather clinicians’ assessments of the SiMAC monitoring system. Results: The response rate was 73.9% (17/23). The data from 40.96% (159/388) of patients with UC treated with aphaeresis was recorded. The main reasons for not including the data from all treated patients were a lack of required data or not meeting the study inclusion criteria. Positive aspects of the SiMAC were identified, as the simplicity of data collection and its systematic, multi-center approach. The negative aspects mentioned were the use of a local computer application and the lack of time for health professionals to enter data. Discussion: The use of monitoring systems helps to formalize the introduction of technologies of little-known effectiveness; involve clinicians and medical societies in coming to agreement and obtaining information about the safety, effectiveness or efficiency of new technologies; and provide relevant information to healthcare administrations for making decisions about the introduction of new technologies into healthcare practice. In order for a monitoring system to work, the process must be straightforward. A minimum set of key variables that are easy to collect must be selected, and an effort made to involve a range of stakeholders, especially institutions and scientific societies, to support the work group

Bifocal reflector antenna system for radar imaging at 300 GHz

A 300 GHz radar imaging system is presented, including descriptions of the radar sensor and antenna subsystems. The antenna consists of a Bifocal Ellipsoidal Gregorian Reflector whose beam is scanned by a combination of the rotation and vertical tilting of a flat small secondary mirror. A prototype is being mounted and its characterization will be presented

Reversible glacial-periglacial transition in response to climate changes and paraglacial dynamics: a case study from Héðinsdalsjökull (northern Iceland)

The objective of this work is to chronologically establish the origin of the different glacial and rock glacier complex landforms deposited by Héðinsdalsjökull glacier (65°39′ N, 18°55′ W), in the Héðinsdalur valley (Skagafjörður fjord, Tröllaskagi peninsula, central northern Iceland). Multiple methods were applied: geomorphological analysis and mapping, glacier reconstruction and equilibrium-line altitude calculation, Cosmic-Ray Exposure dating (in situ cosmogenic 36Cl), and lichenometric dating. The results reveal that a debris-free glacier receded around 6.6 ± 0.6 ka, during the Holocene Thermal Maximum. The retreat of the glacier exposed its headwall and accelerated paraglacial dynamics. As a result, the glacier terminus evolved into a debris-covered glacier and a rock glacier at a slightly higher elevation. The front of this rock glacier stabilized shortly after it formed, although nuclide inheritance is possible, but its sector close the valley head stabilized between 1.5 and 0.6 ka. The lowest part of the debris-covered glacier (between 600 and 820 m altitude) collapsed at ca. 2.4 ka. Since then, periods of glacial advance and retreat have alternated, particularly during the Little Ice Age. The maximum advance during this phase occurred in the 15th to 17th centuries with subsequent re-advances, namely at the beginning of the 19th and 20th centuries. After a significant retreat during the first decades of the 20th century, the glacier advanced in the 1960s to 1990s, and then retreated again, in accordance with the local climatic evolution. The internal ice of both the debris-covered and the rock glacier have survived until the present day, although enhanced subsidence provides evidence of their gradual degradation. A new rock glacier developed from an ice-cored moraine from around 1940–1950 CE. Thus, the Holocene coupling between paraglacial and climatic shifts has resulted in a complex evolution of Héðinsdalsjökull, which is conflicting with previously proposed models: a glacier, which had first evolved into a debris-covered and rock glacier, could later be transformed into a debris-free glacier, with a higher sensitivity to climatic variability.info:eu-repo/semantics/publishedVersio

Financial cost of the admissions for simultaneous pancreas-kidney transplant in a Brazilian Hospital

PURPOSE:To perform a cost analysis of simultaneous pancreas-kidney transplantation (SPKT) in a Brazilian hospital.METHODS:Between January 2008 and December 2011, 105 consecutive SPKTs at the Hospital of Kidney and Hypertension in São Paulo were evaluated. We evaluated the patient demographics, payment source (public health system or supplementary system), and the impact of each hospital cost component. The evaluated costs were corrected to December 2011 values and converted to US dollars.RESULTS:Of the 105 SPKT patients, 61.9% were men, and 38.1% were women. Eight patients died, and 97 were discharged (92.4%). Eighty-nine procedures were funded by the public health system. The cost for the patients who were discharged was $18.352.27; the cost for the deceased patients was$18.449.96 (p = 0.79). The FOR for SPKT during this period was positive at $5,620.65. The costs were distributed as follows: supplies, 36%; administrative costs, 20%; physician fees, 15%; intensive care unit, 10%; surgical center, 10%; ward, 9%.CONCLUSION:Mortality did not affect costs, and supplies were the largest cost component.Federal University of São Paulo Department of SurgeryUniversidade Federal de São Paulo (UNIFESP) Department of SurgeryHypertension and Kidney HospitalUniversidade Federal de São Paulo (UNIFESP) Department of Internal MedicineUniversidade Federal de São Paulo (UNIFESP) Department of PediatricUNIFESP, Department of SurgeryUNIFESP, Department of SurgeryUNIFESP, Department of Internal MedicineUNIFESP, Department of PediatricSciEL

The CSN3 subunit of the COP9 signalosome interacts with the HD region of Sos1 regulating stability of this GEF protein

Sos1 is an universal, widely expressed Ras guanine nucleotide-exchange factor (RasGEF) in eukaryotic cells. Its N-terminal HD motif is known to be involved in allosteric regulation of Sos1 GEF activity through intramolecular interaction with the neighboring PH domain. Here, we searched for other cellular proteins also able to interact productively with the Sos1 HD domain. Using a yeast two-hybrid system, we identified the interaction between the Sos1 HD region and CSN3, the third component of the COP9 signalosome, a conserved, multi-subunit protein complex that functions in the ubiquitin-proteasome pathway to control degradation of many cellular proteins. The interaction of CSN3 with the HD of Sos1 was confirmed in vitro by GST pull-down assays using truncated mutants and reproduced in vivo by co-immunoprecipitation with the endogenous, full-length cellular Sos1 protein. In vitro kinase assays showed that PKD, a COP9 signalosome-associated-kinase, is able to phosphorylate Sos1. The intracellular levels of Sos1 protein were clearly diminished following CSN3 or PKD knockdown. A sizable fraction of the endogenous Sos1 protein was found ubiquitinated in different mammalian cell types. A significant reduction of RasGTP formation upon growth factor stimulation was also observed in CSN3-silenced as compared with control cells. Our data suggest that the interaction of Sos1 with the COP9 signalosome and PKD plays a significant role in maintenance of cellular Sos1 protein stability and homeostasis under physiological conditions and raises the possibility of considering the CSN/PKD complex as a potential target for design of novel therapeutic drugs.We thank R Brent for the pJG45-HeLa library and R. Jorge for help with yeast two-hybrid screening. J.M.R. received grant support from MINECO-FEDER (SAF2016-78852-R), ISCIII-MINECO (FIS-Intrasalud PI13/00703) and Spanish Association against Cancer (AECC). E.S. and A.F.M. were supported by grants from ISCIII-MINECO (FIS PI16/02137), JCyL (SA043U16-UIC 076) and Solorzano Foundation. E.S. and J.M.R. were also supported by ISCIII-RETIC (groups RTICC-RD12/0036/0001 and RTICC-RD12/0036/0021, respectively) and by CIBERONC (groups CB16/12/00352 and CB16/12/00273, respectively). Research co-financed by FEDER funds.S

Relación entre los anticuerpos contra antígenos extraíbles del núcleo y las enfermedades del tejido conectivo identificados por Immunoblots en un hospital y universidad de Lima

Objetivos: determinar la relación de los anticuerpos con los antígenos del núcleo extraíble y las enfermedades del tejido conectivo identificadas por Immunoblot en un hospital de Lima, Perú. Material y métodos: estudio de tipo observacional, ciencias básicas, analíticas y transversales, realizado en el Servicio de Inmunología del Hospital Nacional Arzobispo Loayza entre enero de 2018 y junio de 2018. Analizamos 291 historias clínicas de pacientes con enferme-dad del tejido conectivo y para la detección de anticuerpos contra los antígenos extraíbles del núcleo se empleó el método de Immunoblots

The P34G mutation reduces the transforming activity of K-Ras and N-Ras in NIH 3T3 cells but not of H-Ras

Retraction in The P34G mutation reduces the transforming activity of K-Ras and N-Ras in NIH 3T3 cells but not of H-Ras. [J Biol Chem. 2018]Ras proteins (H-, N-, and K-Ras) operate as molecular switches in signal transduction cascades controlling cell proliferation, differentiation, or apoptosis. The interaction of Ras with its effectors is mediated by the effector-binding loop, but different data about Ras location to plasma membrane subdomains and new roles for some docking/scaffold proteins point to signaling specificities of the different Ras proteins. To investigate the molecular mechanisms for these specificities, we compared an effector loop mutation (P34G) of three Ras isoforms (H-, N-, and K-Ras4B) for their biological and biochemical properties. Although this mutation diminished the capacity of Ras proteins to activate the Raf/ERK and the phosphatidylinositol 3-kinase/AKT pathways, the H-Ras V12G34 mutant retained the ability to cause morphological transformation of NIH 3T3 fibroblasts, whereas both the N-Ras V12G34 and the K-Ras4B V12G34 mutants were defective in this biological activity. On the other hand, although both the N-Ras V12G34 and the K-Ras4B V12G34 mutants failed to promote activation of the Ral-GDS/Ral A/PLD and the Ras/Rac pathways, the H-Ras V12G34 mutant retained the ability to activate these signaling pathways. Interestingly, the P34G mutation reduced specifically the N-Ras and K-Ras4B in vitro binding affinity to Ral-GDS, but not in the case of H-Ras. Thus, independently of Ras location to membrane subdomains, there are marked differences among Ras proteins in the sensitivity to an identical mutation (P34G) affecting the highly conserved effector-binding loop.This work was supported in part by Programa General del Conocimiento (BMC2001-0057), Intramural Instituto de Salud Carlos III (ISCIII) (01/16), and SAF2003-02604 (Ministerio de Ciencia y Tecnología) grants (to J. M. R.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.S