Reconversion of experimental classes of Analytical Chemistry due to the ASPO

En este trabajo se presentan los ajustes realizados en la cátedra de Química Analítica para reconvertir las clases presenciales en clases remotas en el contexto de la pandemia por el COVID-19. El principal desafío fue desarrollar las clases prácticas de laboratorio en forma remota y aun así, promover la adquisición de destrezas y habilidades en el manejo de los instrumentos de laboratorio. Cada trabajo práctico se acompañó de una explicación teórica en formato digitalizado con imágenes secuenciadas y un hipervínculo con videos demostrativos de las partes más significativas de la experiencia dentro del laboratorio. Cada clase conto con un espacio de consulta sincrónica favoreciendo la retroalimentación. Se realizaron evaluaciones formativas utilizando los recursos disponibles en el aula virtual. Este tiempo de ASPO significó para los docentes un aprendizaje intenso que sirvió para reposicionarse y repensar las prácticas, considerando modalidades de enseñanza mixtas en la postpandemia.This work presents the modications that were made in the Analytical Chemistry course to change the classroom lessons into remote ones in the context of the COVID-19 pandemic. The main challenge was to develop practical laboratory classes remotely and yet promote the acquisition of skills and abilities in the handling of laboratory instruments. Each practical work was accompanied by a theoretical explanation in digital format, which contained a brief introduction and a hyperlink with short demonstrative videos for the most remarkable parts of the laboratory experiments. Formative assessments were made using the multiple-choice resource of the virtual classroom. This time of ASPO meant intense learning for the professors, which allowed them to reposition themselves and rethink teaching practices, considering mixed teaching modalities for the post-pandemic.Fil: Baumann, Alicia Jeannette. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Scipioni, Griselda Patricia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Sadañoski, Marcela Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Neis, Emiliano Roberto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Acuña, Miriam Gladys. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentin

Vivências de familiares diante da finitude da criança no processo de adoção de cuidados paliativos

Objetivo: Desvelar a vivência de familiares após notícia da adoção de cuidados paliativos para a criança. Método: Pesquisa fenomenológica na perspectiva de Heidegger. Participaram onze familiares de crianças na Unidade de Terapia Intensiva Pediátrica de hospital universitário do sul do Brasil com indicação de cuidados paliativos. Os depoimentos foram obtidos em entrevista semi-estruturada, de janeiro a novembro/2017, submetidos à análise teóricofilosófica de Heidegger. Pesquisa aprovada pelo Comitê de Ética da instituição. Resultados: A comunicação de cuidados paliativos desencadeia no familiar a percepção da facticidade existencial da criança, descortinando reações explicitadas nas dimensões temáticas: Enfrentando a finitude da criança diante da proposta de cuidados paliativos e Necessidade de cuidado compassivo e solícito. Considerações finais: A fenomenologia permitiu compreender o familiar em seu propósito existencial. A perspectiva compreensiva pode auxiliar a equipe interdisciplinar na comunicação da decisão de cuidados paliativos, de modo sensível e ético, focalizando o melhor interesse da criança. Palavras-chave: Cuidados paliativos. Unidades de terapia intensiva pediátrica. Pesquisa qualitativa

Validity and Prognostic Value of a Polygenic Risk Score for Parkinson’s Disease

Idiopathic Parkinson’s disease (PD) is a complex multifactorial disorder caused by the interplay of both genetic and non-genetic risk factors. Polygenic risk scores (PRSs) are one way to aggregate the effects of a large number of genetic variants upon the risk for a disease like PD in a single quantity. However, reassessment of the performance of a given PRS in independent data sets is a precondition for establishing the PRS as a valid tool to this end. We studied a previously proposed PRS for PD in a separate genetic data set, comprising 1914 PD cases and 4464 controls, and were able to replicate its ability to differentiate between cases and controls. We also assessed theoretically the prognostic value of the PD-PRS, i.e., its ability to predict the development of PD in later life for healthy individuals. As it turned out, the PD-PRS alone can be expected to perform poorly in this regard. Therefore, we conclude that the PD-PRS could serve as an important research tool, but that meaningful PRS-based prognosis of PD at an individual level is not feasible

A Multi-center Genome-wide Association Study of Cervical Dystonia

BACKGROUND: Several monogenic causes for isolated dystonia have been identified, but they collectively account for only a small proportion of cases. Two genome-wide association studies have reported a few potential dystonia risk loci; but conclusions have been limited by small sample sizes, partial coverage of genetic variants, or poor reproducibility. OBJECTIVE: To identify robust genetic variants and loci in a large multicenter cervical dystonia cohort using a genome-wide approach. METHODS: We performed a genome-wide association study using cervical dystonia samples from the Dystonia Coalition. Logistic and linear regressions, including age, sex, and population structure as covariates, were employed to assess variant- and gene-based genetic associations with disease status and age at onset. We also performed a replication study for an identified genome-wide significant signal. RESULTS: After quality control, 919 cervical dystonia patients compared with 1491 controls of European ancestry were included in the analyses. We identified one genome-wide significant variant (rs2219975, chromosome 3, upstream of COL8A1, P-value 3.04 × 10(-8) ). The association was not replicated in a newly genotyped sample of 473 cervical dystonia cases and 481 controls. Gene-based analysis identified DENND1A to be significantly associated with cervical dystonia (P-value 1.23 × 10(-6) ). One low-frequency variant was associated with lower age-at-onset (16.4 ± 2.9 years, P-value = 3.07 × 10(-8) , minor allele frequency = 0.01), located within the GABBR2 gene on chromosome 9 (rs147331823). CONCLUSION: The genetic underpinnings of cervical dystonia are complex and likely consist of multiple distinct variants of small effect sizes. Larger sample sizes may be needed to provide sufficient statistical power to address the presumably multi-genic etiology of cervical dystonia