Hangprinter for large scale additive manufacturing using fused particle fabrication with recycled plastic and continuous feeding

The life cycle of plastic is a key source of carbon emissions. Yet, global plastics production has quadrupled in 40 years and only 9 % has been recycled. If these trends continue, carbon emissions from plastic wastes would reach 15 % of global carbon budgets by 2050. An approach to reducing plastic waste is to use distributed recycling for additive manufacturing (DRAM) where virgin plastic products are replaced by locally manufactured recycled plastic products that have no transportation-related carbon emissions. Unfortunately, the design of most 3-D printers forces an increase in the machine cost to expand for recycling plastic at scale. Recently, a fused granular fabrication (FGF)/fused particle fabrication (FPF) large-scale printer was demonstrated with a GigabotX extruder based on the open source cable driven Hangprinter concept. To further improve that system, here a lower-cost recyclebot direct waste plastic extruder is demonstrated and the full designs, assembly and operation are detailed. The <$1,700 machine’s accuracy and printing performance are quantified, and the printed parts mechanical strength is within the range of other systems. Along with support from the Hangprinter and DUET3 communities, open hardware developers have a rich ecosystem to modify in order to print directly from waste plastic for DRAM

Association of Accelerometry-Measured Physical Activity and Cardiovascular Events in Mobility-Limited Older Adults: The LIFE (Lifestyle Interventions and Independence for Elders) Study.

BACKGROUND:Data are sparse regarding the value of physical activity (PA) surveillance among older adults-particularly among those with mobility limitations. The objective of this study was to examine longitudinal associations between objectively measured daily PA and the incidence of cardiovascular events among older adults in the LIFE (Lifestyle Interventions and Independence for Elders) study. METHODS AND RESULTS:Cardiovascular events were adjudicated based on medical records review, and cardiovascular risk factors were controlled for in the analysis. Home-based activity data were collected by hip-worn accelerometers at baseline and at 6, 12, and 24 months postrandomization to either a physical activity or health education intervention. LIFE study participants (n=1590; age 78.9±5.2 [SD] years; 67.2% women) at baseline had an 11% lower incidence of experiencing a subsequent cardiovascular event per 500 steps taken per day based on activity data (hazard ratio, 0.89; 95% confidence interval, 0.84-0.96; P=0.001). At baseline, every 30 minutes spent performing activities ≥500 counts per minute (hazard ratio, 0.75; confidence interval, 0.65-0.89 [P=0.001]) were also associated with a lower incidence of cardiovascular events. Throughout follow-up (6, 12, and 24 months), both the number of steps per day (per 500 steps; hazard ratio, 0.90, confidence interval, 0.85-0.96 [P=0.001]) and duration of activity ≥500 counts per minute (per 30 minutes; hazard ratio, 0.76; confidence interval, 0.63-0.90 [P=0.002]) were significantly associated with lower cardiovascular event rates. CONCLUSIONS:Objective measurements of physical activity via accelerometry were associated with cardiovascular events among older adults with limited mobility (summary score >10 on the Short Physical Performance Battery) both using baseline and longitudinal data. CLINICAL TRIAL REGISTRATION:URL: http://www.clinicaltrials.gov. Unique identifier: NCT01072500

Motor-sensory cortex-corticospinal system and developing locomotion and placing in rats

Normal and abnormal development of movement in the rat were studied by investigating the growth and organization of the motor-sensory cortexcorticospinal tract system (MSC-CST) and the functional and morphologic effects of ablating the MSC or quadrants of it at different ages. Major growth of the MSC outflow, the CST, in the brain stem and rostral cord occurred in the second and third weeks postnatally, coinciding approximately with the normal mid-third week transition from infantile to mature locomotion. Ablation of the MSC at birth revealed that while the MSC-CST was not essential for ordinary locomotion on flat terrain, its presence hastened normal development of this kind of movement, and that it was absolutely essential for locomotion on difficult terrain. The MSC quadrants showed quite different, and in some domains mutually exclusive, CST projection patterns to forebrain, diencephalon, brain stem, and spinal destinations (determined by Fink-Heimer-Nauta fiber degeneration studies). Ablation of some quadrants produced distinctive syndromes of disordered movement: the posterolateral quadrant related to active grasping in positioning limbs, while the posteromedial quadrant related to tactile motorsensory positioning of limbs. Thus in addition to the classic somatotopic organization of the MSC, there was another kind of organization into regions concerned with components of integrated movement of a number of parts of the body. Several forms of aberrant circuitry developed after MSC ablations in infants, but their possible roles in functional adaptation remain to be determined.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/49673/1/1001430102_ftp.pd

Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

<p>Abstract</p> <p>Background</p> <p>Infestation of ovine skin with the ectoparasitic mite <it>Psoroptes ovis </it>results in a rapid cutaneous immune response, leading to the crusted skin lesions characteristic of sheep scab. Little is known regarding the mechanisms by which such a profound inflammatory response is instigated and to identify novel vaccine and drug targets a better understanding of the host-parasite relationship is essential. The main objective of this study was to perform a combined network and pathway analysis of the <it>in vivo </it>skin response to infestation with <it>P. ovis </it>to gain a clearer understanding of the mechanisms and signalling pathways involved.</p> <p>Results</p> <p>Infestation with <it>P. </it>ovis resulted in differential expression of 1,552 genes over a 24 hour time course. Clustering by peak gene expression enabled classification of genes into temporally related groupings. Network and pathway analysis of clusters identified key signalling pathways involved in the host response to infestation. The analysis implicated a number of genes with roles in allergy and inflammation, including pro-inflammatory cytokines (<it>IL1A, IL1B, IL6, IL8 </it>and <it>TNF</it>) and factors involved in immune cell activation and recruitment (<it>SELE, SELL, SELP, ICAM1, CSF2, CSF3, CCL2 </it>and <it>CXCL2</it>). The analysis also highlighted the influence of the transcription factors NF-kB and AP-1 in the early pro-inflammatory response, and demonstrated a bias towards a Th2 type immune response.</p> <p>Conclusions</p> <p>This study has provided novel insights into the signalling mechanisms leading to the development of a pro-inflammatory response in sheep scab, whilst providing crucial information regarding the nature of mite factors that may trigger this response. It has enabled the elucidation of the temporal patterns by which the immune system is regulated following exposure to <it>P. ovis</it>, providing novel insights into the mechanisms underlying lesion development. This study has improved our existing knowledge of the host response to <it>P. ovis</it>, including the identification of key parallels between sheep scab and other inflammatory skin disorders and the identification of potential targets for disease control.</p