Graded level of nitrogen and mulching effect on growth and yield parameters of tomato in Arghakhanchi, Nepal

A field experiment was carried out to find out the effect of different levels of nitrogen and mulching on growth and yield parameters of tomato (Solanum lycopersicum Mill.) var. VL443 at commercial tomato farm in Sandhikharkha, Arghakhanchi from February – June 2022. The eight treatments were laid out in two factorial RCBD with three replications. The treatment combinations were T1 (Non-mulching with 0 kgha-1), T2 (Non-mulching with 50 kgha-1), T3 (Non- mulching with 100 kgha-1), T4 (Non-mulching with 150 kgha-1), T5 (Mulching with 0 kgha-1), T6 (Mulching with 50 kgha-1), T7 (Mulching with 100 kgha-1), and T8 (Mulching with 150 kgha-1). Growth parameters, yield, and yield attributing traits were recorded. The result indicated that the 150 kgha-1 dose of N application contributes to the higher plant height (178.13 cm), the number of leaves (47.83), fruit length (72.50 mm), fruit diameter (58.83 mm), Individual fruit weight (71.67 g) and yield (2.51 kg/ plant). Similarly, plastic mulch contributes significantly higher plant height (173.6 cm), the number of leaves (47.30), fruit length (68.84 mm), fruit diameter (54.20 mm), Individual fruit weight (72.52 g) and yield (2.53 kg/ plant) as compared to non-mulched condition. Furthermore, fruit yield per hectare in mulching with 150 kg-1 plot was significantly higher in comparison to non-mulching with 0 kgha-1 plot. So, the application of 150 kgha-1 nitrogen along with plastic mulching is recommended to increase the yield of tomatoes under the plastic tunnels in Arghakhanchi

Effect of foliar spray of ethephon doses and pruning intensities on growth, sex expression, and yield of cucumber (var- Bhaktapur local) in Kaski, Nepal

A field experiment was conducted to study the effect of foliar spray of ethephon doses and pruning intensities on the performance of Bhaktapur local cultivar of cucumber in Kaski, Nepal from Feb- May 2021. The study was laid out in Randomized Complete Block design (RCBD) with 2G and 3G cutting, and four different doses of ethephon (@125ppm, @250ppm, @375ppm @500ppm) as six treatments against control and were replicated four times with 28 plots each of 20.8 m2 size having 4 rows with 4 plants per row. The data regarding plant height, leaf number, largest leaf area, days to 1st male and female flowering and fruit harvest, the total number of male and female flowers per plant, M: F flower ratio, fruit length, circumference, weight/fruit, fruit number per plant, fruit set % and yield were recorded and analyzed using MS-Excel and R-studio. The result revealed that growth and yield were significantly influenced by the levels of ethephon and pruning. Highest yield was recorded with ethephon @125ppm (83.54 t ha-1) which is as par with 3G (72.57 t ha-1) which was accompanied by a significantly higher number of fruits per plant {(125ppm=18.33), (3G=15.67)}, average fruit weight {(3G=674gm)}, (125ppm=608gm)}, and fruit set % {(3G=30.66 %)}, (125ppm=24.93 %)}. Significantly higher plant height (149.78 cm), and the highest number of leaves per plant (40) were recorded in control and Ethephon @125ppm at 50 DAT, respectively. The largest leaf area (626.84 cm2) was recorded in 2G which is at par with 3G (613.31 cm2) at 50 DAT. Higher dose of ethephon @500ppm (47.87 days) and 375 ppm (47.17 days) delayed days to 1st male flowering while all ethephon doses (T4=31.83 days, T5=32.5 days, T6=34.81 days, and T7=34.83 days) hastened female flowering than control and pruning. A significantly higher number of male flowers were obtained in the control (89.75), while the number of female flowers and M: F flower ratio increased with increasing ethephon level than in pruning (2G and 3G) and control. Hence, appropriate ethephon (125ppm) dose and pruning (3G) were highly conducive for better growth, sex expression, and yield of cucumber using Bhaktapur Local variety of cucumber in the hilly region of Nepal has a climatic condition similar to Kaski

Response of different wheat varieties to Bipolaris sorokiniana at seedling stage under laboratory condition

Spot blotch (Bipolaris sorokiniana) is a major disease of wheat in warm, humid wheat-growing regions. The aim of present study was to evaluate the infection symptomology and disease severity to different wheat varieties under laboratory condition. A total of ten wheat genotypes including RR-21 (a susceptible check) and Aditya (a resistant check), were evaluated for seedling stage resistance to spot blotch disease through artificial inoculation under laboratory condition at Gokuleswor, Baitadi during 2018. Based on disease severity and area under disease progress curve (AUDPC), Aditya was found resistant and RR-21 was found susceptible The tested genotypes were categorized on the basis of total AUDPC value, where Banganga, BL4341, BL4407 and local genotype were moderately resistant, BL-4406, NL-1190, NL-297 and NL-971 were found moderately susceptible to spot blot at seedling stage. The susceptible check, RR-21 had the highest disease severity (44%) as well as mean AUDPC value (51.38)

Addressing unintentional exclusion of vulnerable and mobile households in traditional surveys in Kathmandu, Dhaka and Hanoi : a mixed methods feasibility study

The methods used in low- and middle-income countries’ (LMICs) household surveys have not changed in four decades; however, LMIC societies have changed substantially and now face unprecedented rates of urbanization and urbanization of poverty. This mismatch may result in unintentional exclusion of vulnerable and mobile urban populations. We compare three survey method innovations with standard survey methods in Kathmandu, Dhaka, and Hanoi and summarize feasibility of our innovative methods in terms of time, cost, skill requirements, and experiences. We used descriptive statistics and regression techniques to compare respondent characteristics in samples drawn with innovative versus standard survey designs and household definitions, adjusting for sample probability weights and clustering. Feasibility of innovative methods was evaluated using a thematic framework analysis of focus group discussions with survey field staff, and via survey planner budgets. We found that a common household definition excluded single adults (46.9%) and migrant-headed households (6.7%), as well as non-married (8.5%), unemployed (10.5%), disabled (9.3%), and studying adults (14.3%). Further, standard two-stage sampling resulted in fewer single adult and non-family households than an innovative area-microcensus design; however, two-stage sampling resulted in more tent and shack dwellers. Our survey innovations provided good value for money, and field staff experiences were neutral or positive. Staff recommended streamlining field tools and pairing technical and survey content experts during fieldwork. This evidence of exclusion of vulnerable and mobile urban populations in LMIC household surveys is deeply concerning and underscores the need to modernize survey methods and practices

Metabolite of the pesticide DDT and incident type 2 diabetes in urban India

BACKGROUND: Previous epidemiological studies, largely conducted in high-income countries and cross-sectional, have suggested a relatively strong association between exposure to dichlorodiphenyldichloroethylene (DDE), a metabolite of the pesticide dichlorodiphenyltrichloroethane (DDT), and type 2 diabetes. DDT is widely used in India and the prevalence of type 2 diabetes there is increasing, but the association between these factors has not been explored to date. OBJECTIVE: The objective was to estimate the association of the p,p' isomer of DDE with incident type 2 diabetes in India. METHODS: A nested case-control study was conducted in a representative prospective cohort of adults from two cities in India. Participants were enrolled in 2010-11 (n = 12,271) and followed for annual assessment of chronic diseases including type 2 diabetes. Baseline plasma samples from incident cases of diabetes (n = 193) and sex-city-matched controls (n = 323) were selected for analysis of p,p-DDE. Odds ratios (OR) and 95% confidence intervals (CI) were estimated using conditional logistic regression. RESULTS: At baseline, cases had higher p,p-DDE concentrations: geometric mean (95% CI) 330 (273-399) ng/g lipid compared to 223 (189-262) ng/g lipid among controls. Delhi participants had higher p,p-DDE concentrations: 579 (521-643) ng/g lipid compared to 122 (102-145) ng/g lipid in Chennai. In unadjusted models, being in the highest versus lowest quartile of p,p-DDE was associated with a more than doubling of the odds of diabetes: unadjusted OR (95% CI), 2.30 (1.19, 4.43). However, this effect was no longer significant after adjustment for age: adjusted (95% CI), 0.97 (0.46, 2.06). DISCUSSION: Results suggest that levels of p,p'-DDE in Delhi are exceptionally high, but we did not observe a significant association between p,p-DDE and incident type 2 diabetes. As this is the first study to evaluate this association in India, more studies are needed to inform our understanding of the association in this context, including potential routes of exposure

Surveys for Urban Equity

This dataset contains results and documentation from three cross-sectional urban household surveys done in Kathmandu (Nepal), Dhaka (Bangladesh) and Hanoi (Vietnam) in 2017 and 2018. The surveys primarily aimed to test the feasibility of using new urban household survey methods that try to better cover/capture informal/slum settlements using sampling frame data generated from random forest models that incorporate census data (which is often outdated and inaccurate) with multiple remotely-sensed covariates, such as urbanisation and infrastructure data. Additionally, the surveys also aimed to gather data on a range of topics including many that are not commonly collected in household surveys, particularly of urban areas: A) basic socio-demographic details of household members, B) household characteristics, assets, income and expenses, C) household migration and social capital, D) household member injury and injury related death, and, for one individual per household, E) migration, social capital and depression/mental health. See the "Readme - dataset file descriptions.docx” file for a description of all files and datasets available, plus additional relevant references

Phenylalanine-Rich Peptides Potently Bind ESAT6, a Virulence Determinant of Mycobacterium tuberculosis, and Concurrently Affect the Pathogen's Growth

BACKGROUND:The secretory proteins of Mycobacterium tuberculosis (M. tuberculosis) have been known to be involved in the virulence, pathogenesis as well as proliferation of the pathogen. Among this set, many proteins have been hypothesized to play a critical role at the genesis of the onset of infection, the primary site of which is invariably the human lung. METHODOLOGY/PRINCIPAL FINDINGS:During our efforts to isolate potential binding partners of key secretory proteins of M. tuberculosis from a human lung protein library, we isolated peptides that strongly bound the virulence determinant protein Esat6. All peptides were less than fifty amino acids in length and the binding was confirmed by in vivo as well as in vitro studies. Curiously, we found all three binders to be unusually rich in phenylalanine, with one of the three peptides a short fragment of the human cytochrome c oxidase-3 (Cox-3). The most accessible of the three binders, named Hcl1, was shown also to bind to the Mycobacterium smegmatis (M. smegmatis) Esat6 homologue. Expression of hcl1 in M. tuberculosis H37Rv led to considerable reduction in growth. Microarray analysis showed that Hcl1 affects a host of key cellular pathways in M. tuberculosis. In a macrophage infection model, the sets expressing hcl1 were shown to clear off M. tuberculosis in much greater numbers than those infected macrophages wherein the M. tuberculosis was not expressing the peptide. Transmission electron microscopy studies of hcl1 expressing M. tuberculosis showed prominent expulsion of cellular material into the matrix, hinting at cell wall damage. CONCLUSIONS/SIGNIFICANCE:While the debilitating effects of Hcl1 on M. tuberculosis are unrelated and not because of the peptide's binding to Esat6-as the latter is not an essential protein of M. tuberculosis-nonetheless, further studies with this peptide, as well as a closer inspection of the microarray data may shed important light on the suitability of such small phenylalanine-rich peptides as potential drug-like molecules against this pathogen

Is evidence-informed urban health planning a myth or reality? Lessons from a qualitative assessment in three Asian cities

City governments are well-positioned to effectively address urban health challenges in the context of rapid urbanization in Asia. They require good quality and timely evidence to inform their planning decisions. In this article, we report our analyses of degree of data-informed urban health planning from three Asian cities: Dhaka, Hanoi and Pokhara. Our theoretical framework stems from conceptualizations of evidence-informed policymaking, health planning and policy analysis, and includes: (1) key actors, (2) approaches to developing and implementing urban health plans, (3) characteristics of the data itself. We collected qualitative data between August 2017 and October 2018 using: in-depth interviews with key actors, document review and observations of planning events. Framework approach guided the data analysis. Health is one of competing priorities with multiple plans being produced within each city, using combinations of top-down, bottom-up and fragmented planning approaches. Mostly data from government information systems are used, which were perceived as good quality though often omits the urban poor and migrants. Key common influences on data use include constrained resources and limitations of current planning approaches, alongside data duplication and limited co-ordination within Dhaka’s pluralistic system, limited opportunities for data use in Hanoi and inadequate and incomplete data in Pokhara. City governments have the potential to act as a hub for multi-sectoral planning. Our results highlight the tensions this brings, with health receiving less attention than other sector priorities. A key emerging issue is that data on the most marginalized urban poor and migrants are largely unavailable. Feasible improvements to evidence-informed urban health planning include increasing availability and quality of data particularly on the urban poor, aligning different planning processes, introducing clearer mechanisms for data use, working within the current systemic opportunities and enhancing participation of local communities in urban health planning