1,383 research outputs found

    Depth-dependent target strengths of gadoids by the boundary-element method

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    Author Posting. © Acoustical Society of America, 2003. This article is posted here by permission of Acoustical Society of America for personal use, not for redistribution. The definitive version was published in Journal of the Acoustical Society of America 114 (2003): 3136-3146, doi:10.1121/1.1619982.The depth dependence of fish target strength has mostly eluded experimental investigation because of the need to distinguish it from depth-dependent behavioral effects, which may change the orientation distribution. The boundary-element method (BEM) offers an avenue of approach. Based on detailed morphometric data on 15 gadoid swimbladders, the BEM has been exercised to determine how the orientation dependence of target strength changes with pressure under the assumption that the fish swimbladder remains constant in shape and volume. The backscattering cross section has been computed at a nominal frequency of 38 kHz as a function of orientation for each of three pressures: 1, 11, and 51 atm. Increased variability in target strength and more abundant and stronger resonances are both observed with increasing depth. The respective backscattering cross sections have been averaged with respect to each of four normal distributions of tilt angle, and the corresponding target strengths have been regressed on the logarithm of fish length. The tilt-angle-averaged backscattering cross sections at the highest pressure have also been averaged with respect to frequency over a 2-kHz band for representative conditions of insonification. For all averaging methods, the mean target strength changes only slightly with depth.This work began with sponsorship by the European Commission through its RTD-program, Contract No. MAS3-CT95-0031 (BASS), and was completed with support by the Office of Naval Research, Contract No. N000140310368

    The Transit Light Curve Project. IX. Evidence for a Smaller Radius of the Exoplanet XO-3b

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    We present photometry of 13 transits of XO-3b, a massive transiting planet on an eccentric orbit. Previous data led to two inconsistent estimates of the planetary radius. Our data strongly favor the smaller radius, with increased precision: R_p = 1.217 +/- 0.073 R_Jup. A conflict remains between the mean stellar density determined from the light curve, and the stellar surface gravity determined from the shapes of spectral lines. We argue the light curve should take precedence, and revise the system parameters accordingly. The planetary radius is about 1 sigma larger than the theoretical radius for a hydrogen-helium planet of the given mass and insolation. To help in planning future observations, we provide refined transit and occultation ephemerides.Comment: To appear in ApJ [22 pages

    Muddled Boundaries of Digital Shrines

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    International audienceBased on an online ethnography study of 274 YouTube videos posted during the Virginia Tech or the Newtown massacres, this article discusses how users resort to participatory media during such mediatized events to create a digital spontaneous shrine. The assemblage of this sanctuary on a website hosting billions of user-generated contents is made possible by means of folksonomy and website architecture, and a two-fold social dynamic based on participatory commitment and the institutionalization of a collective entity. Unlike “physical” spontaneous shrines erected in public spaces, these digital shrines connect the bereaved with provocative or outrageous contributions, notably tributes from school shooting fans using participatory media to commemorate the killer’s memory. This side effect, generated by the technical properties of the platform, compromises the tranquility of the memorial and muddles the boundaries and the contents of such sanctuaries

    The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation

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    We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or resolvases. This method involves two successive rounds of natural transformation using linear DNA: the first introduces a cassette carrying cat (which allows selection by chloramphenicol) and sacB (which allows counter-selection using sucrose) flanked by sequences to either side of the target gene; the second transformation utilises the flanking sequences ligated directly to each other in order to remove the cat-sacB cassette. In order to ensure efficient uptake of the target DNA during transformation, A. pleuropneumoniae uptake sequences are added into the constructs used in both rounds of transformation. This method can be used to generate multiple successive deletions and can also be used to introduce targeted point mutations or insertions of heterologous genes into the A. pleuropneumoniae chromosome for development of live attenuated vaccine strains. So far, we have applied this method to highly transformable isolates of serovars 8 (MIDG2331), which is the most prevalent in the UK, and 15 (HS143). By screening clinical isolates of other serovars, it should be possible to identify other amenable strains

    Protocols for calibrating multibeam sonar

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    Author Posting. © Acoustical Society of America, 2005. This article is posted here by permission of Acoustical Society of America for personal use, not for redistribution. The definitive version was published in Journal of the Acoustical Society of America 117 (2005): 2013-2027, doi:10.1121/1.1869073.Development of protocols for calibrating multibeam sonar by means of the standard-target method is documented. Particular systems used in the development work included three that provide the water-column signals, namely the SIMRAD SM2000/90- and 200-kHz sonars and RESON SeaBat 8101 sonar, with operating frequency of 240 kHz. Two facilities were instrumented specifically for the work: a sea well at the Woods Hole Oceanographic Institution and a large, indoor freshwater tank at the University of New Hampshire. Methods for measuring the transfer characteristics of each sonar, with transducers attached, are described and illustrated with measurement results. The principal results, however, are the protocols themselves. These are elaborated for positioning the target, choosing the receiver gain function, quantifying the system stability, mapping the directionality in the plane of the receiving array and in the plane normal to the central axis, measuring the directionality of individual beams, and measuring the nearfield response. General preparations for calibrating multibeam sonars and a method for measuring the receiver response electronically are outlined. Advantages of multibeam sonar calibration and outstanding problems, such as that of validation of the performance of multibeam sonars as configured for use, are mentioned.Support by the National Science Foundation through Award No. OCE-0002664, NOAA through Grant No. NA97OG0241, and the Cooperative Institute for Climate and Ocean Research (CICOR) through NOAA Contract No. NA17RJ1223 is acknowledged

    A Preliminary Study of Effects of Feral Pig Density on Native Hawaiian Montane Rainforest Vegetation

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    Reports were scanned in black and white at a resolution of 600 dots per inch and were converted to text using Adobe Paper Capture Plug-in.This study aimed to examine the effects of different levels of pig density on native Hawaiian forest vegetation. Pig sign was measured across four pig management units in the ‘Ōla‘a Forest from 1998 through 2004 and pig density estimated based upon pig activity. Six paired vegetation monitoring plots were established in the units, each pair straddling a pig fence. Percent cover and species richness of understory vegetation, ground cover, alien species, and preferred pig forage plants were measured in 1997 and 2003 and compared with pig density estimates. Rainfall and hunting effort and success by management personnel were also tracked over the study period. Vegetation monitoring found a higher percentage of native plants in pig-free or low-pig areas compared to those with medium or high pig densities, with no significant change in the percent native plant species between the first and second monitoring periods. Differences between plots were strongly affected by location, with a higher percentage of native plants in western plots, where pig damage has historically been lower. Expansion of this survey with more plots would help improve the statistical power to detect differences in vegetation caused by pigs. Because of the limited vegetation sampling in this study, the results must be viewed as descriptive. We compare the vegetation within 30 × 30 m plots across three thresholds of historical pig density and show how pig densities can change in unanticipated directions within management units. While these results cannot be extrapolated to area-wide effects of pig activity, these data do contribute to a growing body of information on the impacts of feral pigs on Hawaiian plant communities.was provided by a grant from the Mellon Foundation (administered by The Nature Conservancy) and the Invasive Species Program of the U.S. Geological Survey. Additional support was provided by the National Park Service Inventory and Monitoring Program and the Pacific Cooperative Studies Unit, University of Hawai‘i at Mānoa. Any use of trade, product, or firm names in this publication is for descriptive purposes only and does not imply endorsement by the U.S. Government

    The Exomars Climate Sounder (EMCS) Investigation

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    The ExoMars Climate Sounder (EMCS) investigation is developed at the Jet Propulsion Laboratory (Principal Investigator J. T. Schofield) in collaboration with an international scientific team from France, the United Kingdom and the USA. EMCS plans to map daily, global, pole-to-pole profiles of temperature, dust, water and CO2 ices, and water vapor from the proposed 2016 ExoMars Trace Gas Orbiter (EMTGO). These profiles are to be assimilated into Mars General Circulation Models (MGCMs) to generate global, interpolated fields of measured and derived parameters such as wind

    Probing a Complex of Cytochromecand Cardiolipin by Magnetic Circular Dichroism Spectroscopy: Implications for the Initial Events in Apoptosis

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    Oxidation of cardiolipin (CL) by its complex with cytochrome c (cyt c) plays a crucial role in triggering apoptosis. Through a combination of magnetic circular dichroism spectroscopy and potentiometric titrations, we show that both the ferric and ferrous forms of the heme group of a CL:cyt c complex exist as multiple conformers at a physiologically relevant pH of 7.4. For the ferric state, these conformers are His/Lys- and His/OH–-ligated. The ferrous state is predominantly high-spin and, most likely, His/–. Interconversion of the ferric and ferrous conformers is described by a single midpoint potential of -80 ± 9 mV vs SHE. These results suggest that CL oxidation in mitochondria could occur by the reaction of molecular oxygen with the ferrous CL:cyt c complex in addition to the well-described reaction of peroxides with the ferric form
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