A numerical investigation of coorbital stability and libration in three dimensions

Motivated by the dynamics of resonance capture, we study numerically the coorbital resonance for inclination180 >=I>=0 in the circular restricted three-body problem. We examine the similarities and differences between planar and three dimensional coorbital resonance capture and seek their origin in the stability of coorbital motion at arbitrary inclination. After we present stability maps of the planar prograde and retrograde coorbital resonances, we characterize the new coorbital modes in three dimensions. We see that retrograde mode I (R1) and mode II (R2) persist as we change the relative inclination, while retrograde mode III (R3) seems to exist only in the planar problem. A new coorbital mode (R4) appears in 3D which is a retrograde analogue to an horseshoe-orbit. The Kozai-Lidov resonance is active for retrograde orbits as well as prograde orbits and plays a key role in coorbital resonance capture. Stable coorbital modes exist at all inclinations, including retrograde and polar obits. This result confirms the robustness the coorbital resonance at large inclination and encourages the search for retrograde coorbital companions of the solar system's planets.Comment: accepted for publication in Celestial Mechanics and Dynamical Astronom

Biological activity and cellular uptake of [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complex

Anticancer activity of the new [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] (Me(2)bpy = 4,4'-dimethyl-2,2'-bipyridine) complex was evaluated in vitro against several human cancer cell lines, namely A2780, A2780CisR, HT29, MCF7, MDAMB231 and PC3. Remarkably, the IC50 values, placed in the nanomolar and sub-micromolar range, largely exceeded the activity of cisplatin. Binding to human serum albumin, either HSA (human serum albumin) or HSA(faf) (fatty acid-free human serum albumin) does not affect the complex activity. Fluorescence studies revealed that the present ruthenium complex strongly quench the intrinsic fluorescence of albumin. Cell death by the [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complex was reduced in the presence of endocytosis modulators and at low temperature, suggesting an energy-dependent mechanism consistent with endocytosis. On the whole, the biological activity evaluated herein suggests that the complex could be a promising anticancer agent. (C) 2013 Elsevier Inc. All rights reserved

Comparative Cr, As and CCA induced Cytostaticity in mice kidney: a contribution to assess CCA toxicity

CCA (Chromium Copper Arsenate) treated wood, widely used in outdoor residential structures and playgrounds, poses considerable dangers of leaching of its components to the environment. In this study, mouse kidney samples were used to evaluate the effects of CCA, chromium trioxide (CrO3) and arsenic pentoxide (As2O5) on cell pathophysiology by flow cytometry. Samples were collected after 14, 24, 48 and 96 h of animal exposure. While Cr had no statistically significant cytostatic effects, As2O5 induced a S-phase delay in animals exposed for 24 h, and over time a G0/G1 phase blockage. The effects of CCA in S-phase were similar, but more severe than those of As2O5. Since environmental and public health hazards due to the long durability of CCA-treated wood products, these data confirm that CCA has profoundly toxic effects on cell cycle, distinct from the compounds themselves. These cytostatic effects support cell cycle dynamics as a valuable endpoint to assess the toxicity of remaining CCA-treated infrastructures, and the expected increased waste stream over the coming decades.publishe

Water-sediment physicochemical dynamics in a large reservoir in the Mediterranean region under multiple stressors

Nowadays, the Mediterranean freshwater systems face the threat of water scarcity, along with multiple other stressors (e.g., organic and inorganic contamination, geomorphological alterations, invasive species), leading to the impairment of their ecosystem services. All these stressors have been speeding up, due to climate variability and land cover/land use changes, turning them into a big challenge for the water management plans. The present study analyses the physicochemical and phytoplankton biomass (chlorophyll-a) dynamics of a large reservoir, in the Mediterranean region (Alqueva reservoir, Southern Portugal), under diverse meteorological conditions and land cover/land use real scenarios (2017 and 2018). The most important stressors were identified and the necessary tools and information for a more effective management plan were provided. Changes in these parameters were further related to the observed variations in the meteorological conditions and in the land cover/land use. The increase in nutrients and ions in the water column, and of potentially toxic metals in the sediment, were more obvious in periods of severe drought. Further, the enhancement of nutrients concentrations, potentially caused by the intensification of agricultural activities, may indicate an increased risk of water eutrophication. The results highlight that a holistic approach is essential for a better water resources management strategy

DNA interaction and cytotoxicity studies of new ruthenium(II) cyclopentadienyl derivative complexescontaining heteroaromatic ligands

Four ruthenium(II) complexes with the formula [Ru(eta(5)-C(5)H(5))(PP)L][CF(3)SO(3)], being (PP = two triphenylphosphine molecules), L = 1-benzylimidazole, 1; (PP = two triphenylphosphine molecules), L = 2,2'bipyridine, 2; (PP = two triphenylphosphine molecules), L = 4-Methylpyridine, 3; (PP = 1,2-bis(diphenylphosphine) ethane), L = 4-Methylpyridine, 4, were prepared, in view to evaluate their potentialities as antitumor agents. The compounds were completely characterized by NMR spectroscopy and their crystal and molecular structures were determined by X-ray diffraction. Electrochemical studies were carried out giving for all the compounds quasi-reversible processes. The images obtained by atomic force microscopy (AFM) suggest interaction with pBR322 plasmid DNA. Measurements of the viscosity of solutions of free DNA and DNA incubated with different concentrations of the compounds confirmed this interaction. The cytotoxicity of compounds 1234 was much higher than that of cisplatin against human leukemia cancer cells (HL-60 cells). IC(50) values for all the compounds are in the range of submicromolar amounts. Apoptotic death percentage was also studied resulting similar than that of cisplatin. (C) 2010 Elsevier Inc. All rights reserved

Synthesis and structural characterization of new Piano-stool Ruthenium(II) complexes bearing 1-Butylimidazole heteroaromatic ligand

New cationic ruthenium(II) complexes with the formula [Ru(eta(5)-C5H5)(LL)(1-BuIm)] [Z], with (LL) = 2PPh(3) or DPPE, and Z = CF3SO3-, PF6-, BPh4-, have been synthesized and fully characterized. Spectroscopic and electrochemical studies revealed that the electronic properties of the coordinated 1-butylimidazole were clearly influenced by the nature of the phosphane coligands (LL) and also by the different counter ions. The solid state structures of the six complexes determined by X-ray crystallographic studies, confirmed the expected distorted three-legged piano stool structure. However the geometry of the 1-butylimidazole ligand was found considerably different in all six compounds, being governed by the stereochemistry of the mono and bidentate coligands (PPh3 or DPPE)

Hurdles at work: perceptions of hospital food handlers

<p>Abstract</p> <p>Background</p> <p>Food handlers have a very important role in preventing food contamination during its preparation and distribution. This responsibility is even greater in hospitals, since a large number of patients have low immunity and consequently food contamination by pathogenic bacteria could be particularly harmful. Therefore, a good working environment and periodic training should be provided to food handlers by upper management.</p> <p>Methods</p> <p>This study is qualitative research by means of focus group and thematic content analysis methodologies to examine, in detail, the statements by food handlers working in the milk and specific-diet kitchens in a hospital to understand the problems they face in the workplace.</p> <p>Results</p> <p>We found that food handlers are aware of the role they play in restoring patients' health; they consider it important to offer a good-quality diet. However, according to their perceptions, a number of difficulties prevent them from reaching this aim. These include: upper management not prioritizing human and material resources to the dietetic services when making resource allocation decisions; a perception that upper management considers their work to be of lesser importance; delayed overtime payments; lack of periodic training; managers lacking administrative skills; insufficient dietitian staff assistants, leading to overwork, at the same time as there is an excess of dietitians; unhealthy environmental working conditions – high temperature, high humidity, loud and constant noise level, poor ventilation; lack of food, and kitchen utensils and equipment; and relationship conflicts with chief dieticians and co-workers.</p> <p>Conclusion</p> <p>From these findings, improvement in staff motivation could be achieved by considering non-financial incentives, such as improvement in working conditions and showing appreciation and respect through supervision, training and performance appraisal. Management action, such as investments in intermediary management so that managers have the capacity to provide supportive supervision, as well as better use of performance appraisal and access to training, may help overcome the identified problems.</p

Errors in protein synthesis increase the level of saturated fatty acids and affect the overall lipid profiles of yeast

The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation level of 10-4 is mostly deleterious to yeast, zebrafish and mammal cells. Previous yeast studies have shown that mistranslation affects fitness and deregulates genes related to lipid metabolism, but there is no experimental proof that such errors alter yeast lipid profiles. We engineered yeast strains to misincorporate serine at alanine and glycine sites on a global scale and evaluated the putative effects on the lipidome. Lipids from whole cells were extracted and analysed by thin layer chromatography (TLC), liquid chromatography-mass spectrometry(LC-MS) and gas chromatography (GC). Oxidative damage, fatty acid desaturation and membrane fluidity changes were screened to identify putative alterations in lipid profiles in both logarithmic (fermentative) and post-diauxic shift (respiratory) phases. There were alterations in several lipid classes, namely lyso-phosphatidylcholine, phosphatidic acid, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and triglyceride, and in the fatty acid profiles, namely C16:1, C16:0, C18:1 and C18:0. Overall, the relative content of lipid species with saturated FA increased in detriment of those with unsaturated fatty acids. The expression of the OLE1 mRNA was deregulated, but phospholipid fluidity changes were not observed. These data expand current knowledge of mistranslation biology and highlight its putative roles in human diseases.publishe

Detecting Endometrial Cancer by Blood Spectroscopy: A Diagnostic Cross-Sectional Study

Endometrial cancer is the sixth most common cancer in women, with a rising incidence worldwide. Current approaches for the diagnosis and screening of endometrial cancer are invasive, expensive or of moderate diagnostic accuracy, limiting their clinical utility. There is a need for cost-effective and minimally invasive approaches to facilitate the early detection and timely management of endometrial cancer. We analysed blood plasma samples in a cross-sectional diagnostic accuracy study of women with endometrial cancer (n = 342), its precursor lesion atypical hyperplasia (n = 68) and healthy controls (n = 242, total n = 652) using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy and machine learning algorithms. We show that blood-based infrared spectroscopy has the potential to detect endometrial cancer with 87% sensitivity and 78% specificity. Its accuracy is highest for Type I endometrial cancer, the most common subtype, and for atypical hyperplasia, with sensitivities of 91% and 100%, and specificities of 81% and 88%, respectively. Our large-cohort study shows that a simple blood test could enable the early detection of endometrial cancer of all stages in symptomatic women and provide the basis of a screening tool in high-risk groups. Such a test has the potential not only to differentially diagnose endometrial cancer but also to detect its precursor lesion atypical hyperplasia—the early recognition of which may allow fertility sparing management and cancer prevention

Perfil de pacientes atendidos por la especialidad de endodoncia en el centro de especialidades odontológicas (CEO) de una ciudad de mediano porte del interior del estado de Paraíba

A organização dos serviços públicos de odontologia no Brasil apresenta-se como um desafio, pois a má distribuição e baixa capacidade de oferta tem gerado demanda reprimida nos serviços de saúde bucal, em sua maioria, para tratamento de endodontia. Trata-se de um estudo quantitativo, descritivo, de caráter exploratório e transversal, objetivo do estudo foi avaliar o fluxo e a organização da assistência em endodontia e perfil dos usuários atendidos pelo centro de especialidades odontológicas numa cidade do interior do estado da Paraíba (PB). O universo foi composto por 18.400 prontuários, que correspondem ao número de usuários cadastrados, que já foram ou aguardam atendimento por alguma das especialidades, a amostra foi constituída por 249 prontuários dos pacientes submetidos a endodontia. Observou-se que a maioria dos indivíduos atendidos eram mulheres entre 26 aos 40 anos, os elementos mais submetidos foram, molares e pré-molares. Embora os resultados tenham caracterizado o acesso da assistência ofertada e perfil dos usuários, houve carência do acesso à informações em virtude dos registros estarem incompletos, além disso, não havia informações disponíveis no sistema de regulação do município, para avaliação do fluxo e demanda reprimida ou viabilizar um comparativo dos atendimentos endodônticos realizados nos últimos cinco anos, para analisar se houve melhora no acesso ao serviço. Concluiu-se que há necessidade de melhoria no preenchimento dos prontuários, na referência e contra referência, adoção de protocolos para planejamento do fluxo direcionado por meio de levantamento epidemiológico que expresse a real demanda, bem como, ampliação da oferta do serviço de endodontia.Organization of public dental services in Brazil is a challenge, as poor distribution and low supply capacity have generated pent-up demand for oral health services, mostly for endodontic treatment. This is a quantitative, descriptive, exploratory and cross-sectional study, The aim of the study was to evaluate the flow and organization of endodontic care and the profile of users served by the Dental Specialties Center in a city in the interior of the state of Paraíba (PB). Universe consisted of 18,400 medical records, which correspond to the number of registered users, who have already been or are waiting for care by one of the specialties, the sample consisted of 249 medical records of patients undergoing endodontics. It was observed that most of the individuals attended were women between 26 and 40 years old, the most affected teeth were molars and premolars. Although results characterized access to the assistance offered and the profile of users, there was a lack of access to information due to records being incomplete, in addition, there was no information available in the municipality's regulatory system, to assess flow and repressed demand or enable a comparison of endodontic care performed in the last five years, to analyze whether there was an improvement in access to the service. It was concluded that there is a necessity for improvement in filling of medical records, in reference and counter-reference, adoption of protocols for planning directed flow through an epidemiological survey that expresses the real demand, as well as expanding offer of endodontics service.La organización de los servicios públicos de odontología en Brasil es un desafío, ya que la mala distribución y la baja capacidad de oferta han generado una demanda acumulada de servicios de salud bucal, principalmente para el tratamiento de endodoncia. Se trata de un estudio cuantitativo, descriptivo, exploratorio y transversal, El objetivo del estudio fue evaluar el flujo y la organización de la asistencia endodóntica y el perfil de los usuarios atendidos por el centro de especialidad odontológica en una ciudad del interior del estado de Paraíba (PB). El universo estuvo constituido por 18,400 historias clínicas, que corresponden al número de usuarios registrados, que ya han estado o están en espera de atención por alguna de las especialidades, la muestra estuvo constituida por 249 historias clínicas de pacientes sometidos a endodoncia. Se observó que la mayoría de los individuos atendidos fueron mujeres entre 26 y 40 años, las piezas dentárias más frecuentes fueron molares y premolares. Si bien, los resultados caracterizaron el acceso a la atención ofrecida y el perfil de los usuarios, hubo falta de acceso a la información debido a que los registros estaban incompletos, además, no había información disponible en el sistema normativo del municipio, para evaluar el flujo y demanda reprimida o posibilitar una comparación de la atención endodóncica realizada en los últimos cinco años, para analizar si hubo una mejora en el acceso al servicio. Se concluyó que existe la necesidad de mejorar el llenado de los datos en las historias clínicas, en referencia y contrarreferencia, adopción de protocolos para la planificación del flujo dirigido a través de una encuesta epidemiológica que exprese la demanda real, así como ampliar la oferta del servicio de salud en endodoncia.info:eu-repo/semantics/publishedVersio