Characterisation of the oral glucose and sugar tolerance tests and the enteroinsular axis response in healthy adult donkeys

Background Insulin dysregulation (ID) is diagnosed in horses and ponies using oral glucose (OGTT) and oral sugar (OSTT) tolerance tests. The enteroinsular axis plays a major role in postprandial glucose disposal and insulin response in horses, ponies and foals. The insulin and incretin response to oral carbohydrate challenges has not been characterised in donkeys. Objectives (a) To characterise OGTT and OSTT, and (b) to assess the plasma incretin response to OGTT and OSTT in healthy donkeys. Study design In vivo experiments. Methods Six healthy adult female Andalusian donkeys were challenged with OGTT (1 g/kg glucose, 20% solution by nasogastric tube) and OSTT (0.45 mL/kg corn syrup orally by syringe) with a 1-week washout. Blood samples were collected for glucose (spectrophotometry), insulin (radioimmunoassay), glucose-dependent insulinotropic polypeptide (GIP, ELISA) and active glucagon-like peptide-1 (aGLP-1, ELISA) determination over 6 hours. Curves were analysed and proxies calculated. Results Glucose and insulin concentrations peaked at 180 minutes in OGTT, but at 300 and 150 minutes in OSTT, respectively. Plasma GIP concentrations increased in the OGTT and OSTT (peaked at 180 and 360 minutes, respectively), but aGLP-1 increased only in OGTT (240 minutes). Main limitations Single breed, narrow age and sample, diet, season and not having donkeys with evidence of ID to provide clinical validation. Conclusions Donkeys have a functional enteroinsular axis that is activated by enteral carbohydrates. Donkeys have evident endocrine differences with horses, supporting the validation of the OSTT and OGTT to assess insulin sensitivity in this species to avoid extrapolation from horses

Cardiomiopatía hipertrófica: experiencia de 5 años en un centro cardiovascular

ResumenIntroducciónLa cardiomiopatía hipertrófica se define como el engrosamiento de la pared ventricular izquierda que no es explicado por condiciones anormales de la carga y que impone un riesgo de: arritmias, falla cardiaca y muerte súbita.Se pretende identificarLa prevalencia, las características clínicas y del tratamiento de los pacientes con cardiomiopatía hipertrófica atendidos en nuestra institución.Materiales y métodosSe extrajeron y analizaron los registros de pacientes con cardiomiopatías hipertróficas, definiendo las características generales y explorando las diferencias entre subgrupos, todos los contrastes estadísticos asumieron una confianza del 95%.ResultadosSe evaluaron 22 casos de la cardiomiopatía hipertrófica, el sexo femenino aportó el 40,9% del total de los casos, la edad promedio fue 54 años. El 77,27% presentaron insuficiencia mitral, el 63,64% reportaron dolor torácico, el 68,18% se encontraban en tratamiento inhibidor de renina y el 95,45% tenían betabloqueador, el grosor promedio del septum fue del 22,77mm, la prevalencia de fibrilación auricular fue del 22,7% y la de enfermedad coronaria del 18%.ConclusionesLa prevalencia de la cardiomiopatía hipertrófica en el período 2009-2013 fue del 2,35%, relacionado principalmente con la insuficiencia mitral y tenían antecedente de dolor torácico, la prevalencia de la fibrilación auricular en pacientes con cardiomiopatía hipertrófica en el presente estudio fue del 22,7% y la de enfermedad coronaria del 18%.AbstractIntroductionHypertrophic cardiomyopathy is defined as the thickening of the left ventricular wall that is not explained by abnormal loading conditions, imposing a risk for arrhythmias, heart failure and sudden death.We attempt to identify the prevalence, clinical features and treatment of patients with hypertrophic cardiomyopathy assisted within our institution.Material and methodsRecords of patients with hypertrophic cardiomyopathy were pulled and analysed, defining general features and exploring differences among subgroups; all statistical contrasts assumed a confidence of 95%.Results22 cases of hypertrophic cardiomyopathy were assessed, 40.9% of whom were female, average age was 54. 77.27% showed mitral insufficiency, 63.4% reported chest pain, 68.18% were receiving renin inhibitor therapy and 95.45% were taking beta blockers. Average septum thickness was 22.77mm, prevalence of atrial fibrillation was 22.7% and coronary disease 18%.ConclusionPrevalence of hypertrophic cardiomyopathy during the 2009-2013 period was of 2.35%, mostly related to mitral insufficiency and previous history of chest pain; prevalence of atrial fibrillation in patients with hypertrophic cardiomyopathy in our study was of 22.7%, and 28% for coronary disease

Weak localization and electron-electron interaction in the layered compound CuFeTe2

The study of the electrical properties of the layered compound CuFeTe2 shows that there are three well differentiated conduction regimes depending on the temperature. Below TSDW ~ 300 K the formation of a Spin Density Wave (SDW) state has been reported, in the frame of a metal to non metal transition. Below 100 K (~ TSDW/3) the behavior of the electrical resistance as a function of temperature and magnetic field is attributable to the still present not condensed electrons (quasi particles) in the SDW state. At low temperatures (1.8 - 20K), low current (< 1 mA) and magnetic eld (0<H <6 Tesla), the effects of weak localization and electronic interactions in two dimensions appear. At intermediate temperatures (20 < T < 100 K) a hopping conductivity behavior is observed

Biodegradation of phenolic compounds present in oil-mill wastewater

Las aguas residuales de la obtención del aceite de oliva o alpechines son responsables de la mayor contaminación medioambiental de Andalucía por su elevada carga de materia orgánica. Su depuración por biometanización es difícil debido a la actividad antimicrobiana de los compuestos fenólícos. Este tipo de sustancias puede eliminarse en gran parte tratando previamente el alpechín por vía aerobia con microorganismos específicos, después de lo cual la depuración anaerobia tiene lugar sin inhibiciones, siendo la constante de velocidad cinco veces superior a la de antes del pretratamiento. En este trabajo se hace un estudio cualitativo de los fenoles por cromatografía en capa fina y cuantitativo de los fenoles totales, orto- y meta-difenoles, así como de la Demanda Química de Oxígeno del alpechín antes del tratamiento, después del pretratamiento y después de la biometanización. Se ha comprobado también la ausencia de leucoantocianinas que pudieran dar origen a fenoles polimerizadosOil-mill wastewater is the main responsible of the pollution in Andalusian due to high content of organic substances. Its depuration by methanization is difficult because of the antimicrobial activity of phenolic derivatives. These compounds can be drastically reduced by a previous aerobic treatment of the oilmill wastewater with specific microorganisms. After this pretreatment, anaerobic depuration takes place without inhibition, the velocity of the reaction being 5 times higher than before the pretreatment. In this paper a qualitative study of the phenols present in oil-mill wastewater by thin layer chromatography has been done. Beside that, quantitative determination of total phenols, ortoand meta-diphenols as well as Chemical Oxigen Demand (COD) before the treatment, after the pretreatment and after biomethanization have been carried out. Finally, leucoanthocyanins that could originate polymerized phenols have not been foun

Kinetics of the anaerobic purification of aerobically prebiotreated olive mill wastewater

Se ha efectuado un estudio cinético del proceso de depuración anaerobia de un alpechín previamente tratado vía aerobia con objeto de eliminar la mayor parte de los compuestos fenólicos responsables de su poder inhibidor. Se utilizan dos reactores anaerobios, uno con soporte Pansil y un Testigo sin relleno, para estudiar la influencia del soporte de inmovilización tanto sobre las constantes biocinéticas como sobre la eficiencia del proceso de digestión anaerobia o biometanización de este alpechín pretratado. A diferencia de lo que ocurre con el alpechín natural, se observa sólo una pequeñísima disminución de las constantes aparentes de velocidad del proceso anaerobio al aumentar la densidad de carga. Por otra parte, no se aprecian fenómenos de inhibición en el proceso de biometanización de este agua residual previamente tratada de forma aerobiaA kinetic study has been carried out on the process of anaerobic purification of an olive mill wastewater pre-treated aerobically to eliminate the greater part of the phenolic compounds responsible for inhibition. Two anaerobic digesters were used, one with Pansil support, and the other a Reference without filling. The aim was to study the influence of the immobilization support both on the biokinetic constants and on the efficiency of the anaerobic digestion process or biomethanization of this pre-treated olive mill wastewater. In contrast to what takes place with natural olive mill wastewater, only a very small decrease in the apparent rate constants of the anaerobic process was observed on increasing load density. At the same time, no inhibition was found in the biomethanization of this aerobically pre-treated wastewate

Contribución al conocimiento de la pteridoflora de Andalucía: sobre el género Ophioglossum L. en Almería

Contribution to the knowledge about Andalusian pteridophyte flora: on the genus Ophiglossum L. inAlmeríaPalabras clave. Biodiversidad, Corología, Montañas Béticas, Sureste ibérico.Key words. Biodiversity, Chorology, Baetic Ranges, South-eastern Iberian Peninsula

Effect of 4-nonylphenol on the performance and microbial community of a sequencing batch reactor

[EN] 4-nonylphenol (4-NP) is one of the most relevant endocrine-disrupting compounds that can be found in wastewaters. In this work, the effect of dosing 1 mg center dot L 1 of 4-NP to simulated wastewater on the activated sludge process was assessed. For it, two laboratory sequencing batch reactors (SBR) were operated for 94 days, adding 1 mg center dot L 1 of 4-NP to the wastewater entering one of them (SBR-NP), while the other one (SBR-B) worked as a control reactor. Holistic study of 4-NP influence on activated sludge treatment was carried out, which included both the evolution of the biomass characteristics and the effect of this substance on reactor performance. Although the COD removal efficiency in SBR-NP was lower than in the reactor without 4-NP addition (SBR-B), COD removal efficiency of SBR-NP was always higher than 90%. From day 50, nitrification bacteria were inhibited in SBR-NP and cellular viability decreased from 85.7 +/- 11.0% in the first 50 days to 63.0 +/- 10.2% in the last 44 days. Concerning the microbial community analysis, both Nitrosomonas and Nitrospira abundances decreased in SBR-NP (from 0.62% to 0.45%, and from 2.39% to 1.01%, respectively). Proteobacteria abundance was considerably higher in SBR-NP at the end of the experiment (44.28% in SBR-NP and 25.88% in SBR-B), which was due to increase of Aquabacterium genus (13.00% and 0.00% in SBR-NP and SBR-B, respectively), playing an important role in 4-NP degradation. Thus, 4-NP presence, in the concentrations studied, affected heterotrophic and autotrophic bacteria differently, having a negative effect in the second group.Acknowledgements Authors thank the Spanish Ministry of Science, Innovation and Universities for the financial support (Reference of the project: RTI2018-096916-B-I00) .Ferrer-Polonio, E.; Fernández-Navarro, J.; Mendoza Roca, JA.; Bes-Piá, M.; Alonso Molina, JL. (2022). Effect of 4-nonylphenol on the performance and microbial community of a sequencing batch reactor. Journal of Environmental Chemical Engineering. 10(2):1-9. https://doi.org/10.1016/j.jece.2022.1072491910

Aprovechamiento del bagazo de piña para obtener celulosa y bioetanol

Actualmente se están buscando nuevas alternativas energéticas a partir de biomasa, recursos renovables y desechos agroindustriales, para desarrollar nuevas tecnologías y procesos en la obtención de biocombustibles. El objetivo fue obtener celulosa y bio-etanol del bagazo de piña (desecho agrícola). El aprovechamiento de este bagazo evitará el consumo de cultivos destinados a la alimentación, evitando el uso excesivo de tierras y el empleo de residuos orgánicos agroindustriales. Además, el producto obtenido tiene un valor agregado y podría convertirse en un beneficio para los productores de piña. La finalidad fue estudiar un proceso para extraer celulosa del bagazo de piña, y mediante hidrólisis ácida de celulosa y bagazo se obtuvo glucosa. Esta glucosa se neutralizó a pH de 5 y se realizó la fermentación en un medio anaeróbico, utilizando el microorganismo Saccharomyces cerevisiae, variando tiempos de fermentación (36, 40, 48 y 72 h) y manteniendo la temperatura a 30ºC. La celulosa obtenida presentó una conversión del 60% y mediante FTIR se corroboró que la celulosa fue tipo II. Se obtuvo bio-etanol mediante destilación, presentando un rendimiento del 35% con bagazo y del 57% con celulosa con un tiempo de fermentación de 48 y 72 h, respectivamente

Intensive Habitat Loss in South Spain: Arborescent Scrubs with <em>Ziziphus</em> (5220*)

The habitat arborescent matorral with Ziziphus (5220*) was included in the Habitats Directive of the European Commission. These plant formations represent the maximum potential vegetation in a very restrictive arid environment, since it encompasses endemic, tropical, or Maghrebian floristic elements, and from other areas of the ancient Thetis Sea. In fact, the version of this community with Gymnosporia senegalensis (Lam.) Loes. [=Maytenus senegalensis (Lam.) Exell] constitutes extraordinarily singular flora formations in the Iberian southeast. These are unique communities in Europe and ecologically extremely valuable and, however, have been included among the Europe’s most endangered habitats. The vast economic development experienced in South Spain based on the remarkable transformation of traditional farming patterns into a highly profitable agriculture that uses industrial production methods and the groundwater intensively (agriculture intensification and land-use change), in addition to urbanization without sustainable land planning, determines that European G. senegalensis populations are seriously threatened by severe habitat destruction and fragmentation