Identification and functional characterization of a highly divergent N-acetylglucosaminyltransferase I (TbGnTI) in <em>Trypanosoma brucei</em>

Trypanosoma brucei expresses a diverse repertoire of N-glycans, ranging from oligomannose and paucimannose structures to exceptionally large complex N-glycans. Despite the presence of the latter, no obvious homologues of known β1–4-galactosyltransferase or β1–2- or β1–6-N-acetylglucosaminyltransferase genes have been found in the parasite genome. However, we previously reported a family of putative UDP-sugar-dependent glycosyltransferases with similarity to the mammalian β1–3-glycosyltransferase family. Here we characterize one of these genes, TbGT11, and show that it encodes a Golgi apparatus resident UDP-GlcNAc:α3-d-mannoside β1–2-N-acetylglucosaminyltransferase I activity (TbGnTI). The bloodstream-form TbGT11 null mutant exhibited significantly modified protein N-glycans but normal growth in vitro and infectivity to rodents. In contrast to multicellular organisms, where the GnTI reaction is essential for biosynthesis of both complex and hybrid N-glycans, T. brucei TbGT11 null mutants expressed atypical “pseudohybrid” glycans, indicating that TbGnTII activity is not dependent on prior TbGnTI action. Using a functional in vitro assay, we showed that TbGnTI transfers UDP-GlcNAc to biantennary Man(3)GlcNAc(2), but not to triantennary Man(5)GlcNAc(2), which is the preferred substrate for metazoan GnTIs. Sequence alignment reveals that the T. brucei enzyme is far removed from the metazoan GnTI family and suggests that the parasite has adapted the β3-glycosyltransferase family to catalyze β1–2 linkages

The Globular Cluster Systems in the Coma Ellipticals. III: The Unique Case of IC 4051

Using archival \hst WFPC2 data, we derive the metallicity distribution, luminosity function, and spatial structure of the globular cluster system around IC 4051, a giant E galaxy on the outskirts of the Coma cluster core. The metallicity distribution derived from the (V-I) colors has a mean [Fe/H] = -0.3, a near-complete lack of metal-poor clusters, and only a small metallicity gradient with radius; it may, however, have two roughly equal metallicity subcomponents, centered at [Fe/H] ~ 0.0 and -1.0. The luminosity distribution (GCLF) has the Gaussian-like form observed in all other giant E galaxies, with a peak (turnover) at V = 27.8, consistent with a Coma distance of 100 Mpc. The radial profiles of both the GCS and the halo light show an unusually steep falloff which may indicate that the halo of this galaxy has been tidally truncated. Lastly, the specific frequency of the GCS is remarkably large: we find S_N = 11 +- 2, resembling the central cD-type galaxies even though IC 4051 is not a cD or brightest cluster elliptical. A formation model consistent with most of the observations would be that this galaxy was subjected to removal of a large fraction of its protogalactic gas shortly after its main phase of globular cluster formation, probably by its first passage through the Coma core. Since then, no significant additions due to accretions or mergers have taken place.Comment: 24 pp. plus 13 Figures. Postscript file for the complete paper can also be downloaded from http://www.physun.mcmaster.ca/~harris/WEHarris.html. Astron.J., in pres

NGC 770: A Counter-Rotating Core in a Low-Luminosity Elliptical Galaxy

We present evidence for a counter-rotating core in the low-luminosity (M_B = -18.2) elliptical galaxy NGC 770 based on internal stellar kinematic data. This counter-rotating core is unusual as NGC 770 is not the primary galaxy in the region and it lies in an environment with evidence of on-going tidal interactions. We discovered the counter-rotating core via single-slit Keck/ESI echelle spectroscopy; subsequent integral field spectroscopy was obtained with the Gemini/GMOS IFU. The counter-rotating region has a peak rotation velocity of 21 km/s as compared to the main galaxy's rotation speed of greater than 45 km/s in the opposite direction. The counter-rotating region extends to a radius of 4'' (0.6 kpc), slightly smaller than the half-light radius of the galaxy which is 5.3'' (0.8 kpc) and is confined to a disk whose scale height is less than 0.8'' (0.1 kpc). We compute an age and metallicity of the inner counter-rotating region of 3 +/- 0.5 Gyr and [Fe/H] = 0.2 +/- 0.2 dex, based on Lick absorption-line indices. The lack of other large galaxies in this region limits possible scenarios for the formation of the counter-rotating core. We discuss several scenarios and favor one in which NGC 770 accreted a small gas-rich dwarf galaxy during a very minor merging event. If this scenario is correct, it represents one of the few known examples of merging between two dwarf-sized galaxies.Comment: 26 pages, 9 figures. Accepted to AJ. See this http://www.ociw.edu/~mgeha/geha.ps.gz for version with high resolution figure

The FORS Deep Field Spectroscopic Survey

We present a catalogue and atlas of low-resolution spectra of a well defined sample of 341 objects in the FORS Deep Field. All spectra were obtained with the FORS instruments at the ESO VLT with essentially the same spectroscopic set-up. The observed extragalactic objects cover the redshift range 0.1 to 5.0. 98 objects are starburst galaxies and QSOs at z > 2. Using this data set we investigated the evolution of the characteristic spectral properties of bright starburst galaxies and their mutual relations as a function of the redshift. Significant evolutionary effects were found for redshifts 2 < z < 4. Most conspicuous are the increase of the average C IV absorption strength, of the dust reddening, and of the intrinsic UV luminosity, and the decrease of the average Ly alpha emission strength with decreasing redshift. In part the observed evolutionary effects can be attributed to an increase of the metallicity of the galaxies with cosmic age. Moreover, the increase of the total star-formation rates and the stronger obscuration of the starburst cores by dusty gas clouds suggest the occurrence of more massive starbursts at later cosmic epochs.Comment: 24 pages, 25 figures (35 PS files), 4 tables, accepted for publication in A&A. v2: minor typos corrected and references update

Nuclear stellar discs in low-luminosity elliptical galaxies: NGC 4458 and NGC 4478

We present the detection of nuclear stellar discs in the low-luminosity elliptical galaxies NGC 4458 and NGC 4478, which are known to host a kinematically-decoupled core. Using archival HST imaging, and available absorption line-strength index data based on ground-based spectroscopy, we investigate the photometric parameters and the properties of the stellar populations of these central structures. Their scale length, h, and face-on central surface brightness, mu_0^c, fit on mu_0^c-h relation for galaxy discs. For NGC 4458 these parameters are typical for nuclear discs, while the same quantities for NGC 4478 lie between those of nuclear discs and the discs of discy ellipticals. We present Lick/IDS absorption line-strength measurements of Hbeta, Mgb, along the major and minor axes of the galaxies. We model these data with simple stellar populations that account for the alpha/Fe overabundance. The counter-rotating central disc of NGC 4458 is found to have similar properties to the decoupled cores of bright ellipticals. This galaxy has been found to be uniformly old despite being counter-rotating. In contrast, the cold central disc of NGC 4478 is younger, richer in metals and less overabundant than the main body of the galaxy. This points to a prolonged star formation history, typical of an undisturbed disc-like, gas-rich (possibly pre-enriched) structure.Comment: 11 pages, 8 figures, accepted for pubblication on MNRA

The Bulge-Disk Orthogonal Decoupling in Galaxies: NGC 4698

The R-band isophotal map of the Sa galaxy NGC 4698 shows that the inner region of the bulge structure is elongated perpendicularly to the major axis of the disk, this is also true for the outer parts of the bulge if a parametric photometric decomposition is adopted. At the same time the stellar component is characterized by an inner velocity gradient and a central zero-velocity plateau along the minor and major axis of the disk respectively. This remarkable geometric and kinematic decoupling suggests that a second event occurred in the formation history of this galaxy.Comment: 12 pages, LaTex, with 4 PostScript figures. Accepted for publication in The Astrophysical Journal Letter

The Tully-Fisher relation at intermediate redshift

Using the Very Large Telescope in Multi Object Spectroscopy mode, we have observed a sample of 113 field spiral galaxies in the FORS Deep Field (FDF) with redshifts in the range 0.1<z<1.0. The galaxies were selected upon apparent brightness (R<23) and encompass all late spectrophotometric types from Sa to Sdm/Im. Spatially resolved rotation curves have been extracted for 77 galaxies and fitted with synthetic velocity fields taking into account all observational effects from inclination and slit misalignment to seeing and slit width. We also compared different shapes for the intrinsic rotation curve. To gain robust values of V_max, our analysis is focussed on galaxies with rotation curves which extend well into the region of constant rotation velocity at large radii. If the slope of the local Tully-Fisher relation (TFR) is held fixed, we find evidence for a mass-dependent luminosity evolution which is as large as up to 2 mag for the lowest-mass galaxies, but is small or even negligible for the highest-mass systems in our sample. In effect, the TFR slope is shallower at z~0.5 in comparison to the local sample. We argue for a mass-dependent evolution of the mass-to-light ratio. An additional population of blue, low-mass spirals does not seem a very appealing explanation. The flatter tilt we find for the distant TFR is in contradiction to the predictions of recent semi-analytic simulations.Comment: 18 pages, 14 figures, A&A, in press. Section on sample completeness added. Please note that the entire analysis is based on undisturbed, high quality rotation curves! Potential effects of tidal interactions are also discusse

The Tully-Fisher relation for S0 galaxies

We present a study of the local B and K-band Tully-Fisher Relation (TFR) between absolute magnitude and maximum circular speed in S0 galaxies. To make this study, we have combined kinematic data, including a new high-quality spectral data set from the Fornax Cluster, with homogeneous photometry from the RC3 and 2MASS catalogues, to construct the largest sample of S0 galaxies ever used in a study of the TFR. Independent of environment, S0 galaxies are found to lie systematically below the TFR for nearby spirals in both optical and infrared bands. This offset can be crudely interpreted as arising from the luminosity evolution of spiral galaxies that have faded since ceasing star formation. However, we also find a large scatter in the TFR. We show that most of this scatter is intrinsic, not due to the observational uncertainties. The presence of such a large scatter means that the population of S0 galaxies cannot have formed exclusively by the above simple fading mechanism after all transforming at a single epoch. To better understand the complexity of the transformation mechanism, we have searched for correlations between the offset from the TFR and other properties of the galaxies such as their structural properties, central velocity dispersions and ages (as estimated from line indices). For the Fornax Cluster data, the offset from the TFR relates with the estimated age of the stars in the individual galaxies, in the sense and of the magnitude expected if S0 galaxies had passively faded since being converted from spirals. This correlation implies that a significant part of the scatter in the TFR arises from the different times at which galaxies began their transformation.Comment: 17 pages, 11 figures, 3 tables, accepted for publication in MNRA