Impacts of Early Holocene environmental dynamics on open-air occupation patterns in the Western Mediterranean: insights from El Arenal de la Virgen (Alicante, Spain)

Open-air sites represent a fundamental proxy of the Early Holocene adaptive systems in the Iberian Peninsula. However, its research potential for the study of human–environmental interactions has been minimally explored. In this work, we present the results of an integrated research programme focused on open-area excavations at the Mesolithic site of Arenal de la Virgen (Alicante, Spain). Novel multi-scalar geoarchaeological and archaeo-stratigraphic studies, coupled with featured-based palaeobotanical analysis, were used to design an extensive radiocarbon dating programme and produce different Bayesian chronological models. Our results distinguish two different Mesolithic occupation phases, dating to 9.3–9.1 and 8.6–8.3k cal a bp respectively, consisting of combustion features and lithic scatters. The comparison of occupational dynamics with the nearby palaeoecological records of Salines and Villena indicated that both Mesolithic phases occurred under relatively stable environmental conditions. The second Mesolithic phase, however, ended during the onset of the 8.2k cal a bp climatic event, when sedimentation processes shifted from soil formation to accretion of aeolian sands. We demonstrate that the end of the Mesolithic occupations at Arenal de la Virgen coincides with the cessation of radiocarbon-dated activity in other open-air Postglacial sites in the central Mediterranean region of Iberia.This research is primarily part of a project that has received funding from the European Research Council (ERC) under the Horizon 2020 research and innovation programme (Grant agreement No. 683018) to JFLdP. Additional analyses on the pollen data sets have been produced in the context of the research project HAR2017-88503-P supported by the Spanish Ministry of Science and Innovation. JFLdP is additionally supported by the Plan Gen-T programme (Ref.CIDEGENT-18/040) from the Generalitat Valenciana. JRR is currently supported by a Margarita Salas fellowship (ref. MARSALAS21-22) at the University of Alicante, and AP-D is holder of a María Zambrano fellowship at the University of the Basque Country (UPV/EHU) both funded by the European Union-Next Gene and the Ministry of Universities (Government of Spain)

Screening risk assessment tools for assessing the environmental impact in an abandoned pyritic mine in Spain

This is the author’s version of a work that was accepted for publication in Science of the Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Science of the Total Environment 409.4 (2011): 692-703 http://dx.doi.org/10.1016/j.scitotenv.2010.10.056This paper describes a new methodology for assessing site-specific environmental impact of contaminants. The proposed method integrates traditional risk assessment approaches with real and variable environmental characteristics at a local scale. Environmental impact on selected receptors was classified for each environmental compartment into 5 categories derived from the whole (chronic and acute) risk assessment using 8 risk levels. Risk levels were established according to three hazard quotients (HQs) which represented the ratio of exposure to acute and chronic toxicity values. This tool allowed integrating in only one impact category all the elements involved in the standard risk assessment. The methodology was applied to an abandoned metal mine in Spain, where high levels of As, Cd, Zn and Cu were detected. Risk affecting potential receptors such as aquatic and soil organisms and terrestrial vertebrates were assessed. Whole results showed that impact to the ecosystem is likely high and further investigation or remedial actions are necessary. Some proposals to refine the risk assessment for a more realistic diagnostic are included.This work has been financed by Madrid Community through EIADES Project S-505/AMB/0296, and by Spanish MinistryfEducation and Science, project CTM-2007-66401-CO2/TECN

Relational aggression in adolescents with conduct disorder:sex differences and behavioral correlates

As most research on conduct disorder (CD) has been conducted on male participants, it has been suggested that female-specific symptoms may be underestimated based on current DSM-5 criteria. In particular, relational aggression, i.e. the hurtful, often indirect, manipulation of relationships with the intention of damaging the other’s social position, has been proposed as a characteristic of CD that is more common in females. In addition, sex-specific studies on correlates of relational aggressive behavior are lacking. Relational aggression may be strongly related to the correlates of proactive aggression, namely low affective empathy, and high levels of callous-unemotional (CU) traits and relational victimization. Thus, the present study investigated sex differences in relational aggression, and associations between relational aggression and correlates of proactive aggression in 662 adolescents with CD (403 females) and 849 typically-developing controls (568 females) aged 9–18 years (M = 14.74, SD = 2.34) from the European multi-site FemNAT-CD study. Females with CD showed significantly higher levels of relational aggression compared to males with CD, whereas no sex differences were seen in controls. Relational aggression was only partly related to correlates of proactive aggression in CD: Independent of sex, CU traits showed a positive association with relational aggression. In females only, cognitive, but not affective empathy, was negatively associated with relational aggression. Relational victimization was more strongly associated with relational aggression in males compared to females. Despite interesting sex specific correlates of relational aggression, effects are small and the potential clinical implications should be investigated in future studies

Multidrug-Resistant Tuberculosis in Europe, 2010-2011

This study (TBNET #30) was supported by the European Commission Seventh Framework Programme (FP7/2007-2013) under grant agreement FP7-223681 and was part of the EU FP7–funded TBPANNET project (http://www.tbpannet.org). C.L. is supported by the German Center for Infection Research

EphA3 Expressed in the Chicken Tectum Stimulates Nasal Retinal Ganglion Cell Axon Growth and Is Required for Retinotectal Topographic Map Formation

BACKGROUND: Retinotopic projection onto the tectum/colliculus constitutes the most studied model of topographic mapping and Eph receptors and their ligands, the ephrins, are the best characterized molecular system involved in this process. Ephrin-As, expressed in an increasing rostro-caudal gradient in the tectum/colliculus, repel temporal retinal ganglion cell (RGC) axons from the caudal tectum and inhibit their branching posterior to their termination zones. However, there are conflicting data regarding the nature of the second force that guides nasal axons to invade and branch only in the caudal tectum/colliculus. The predominant model postulates that this second force is produced by a decreasing rostro-caudal gradient of EphA7 which repels nasal optic fibers and prevents their branching in the rostral tectum/colliculus. However, as optic fibers invade the tectum/colliculus growing throughout this gradient, this model cannot explain how the axons grow throughout this repellent molecule. METHODOLOGY/PRINCIPAL FINDINGS: By using chicken retinal cultures we showed that EphA3 ectodomain stimulates nasal RGC axon growth in a concentration dependent way. Moreover, we showed that nasal axons choose growing on EphA3-expressing cells and that EphA3 diminishes the density of interstitial filopodia in nasal RGC axons. Accordingly, in vivo EphA3 ectodomain misexpression directs nasal optic fibers toward the caudal tectum preventing their branching in the rostral tectum. CONCLUSIONS: We demonstrated in vitro and in vivo that EphA3 ectodomain (which is expressed in a decreasing rostro-caudal gradient in the tectum) is necessary for topographic mapping by stimulating the nasal axon growth toward the caudal tectum and inhibiting their branching in the rostral tectum. Furthermore, the ability of EphA3 of stimulating axon growth allows understanding how optic fibers invade the tectum growing throughout this molecular gradient. Therefore, opposing tectal gradients of repellent ephrin-As and of axon growth stimulating EphA3 complement each other to map optic fibers along the rostro-caudal tectal axis

Phytostabilization of metals in mine soils using Brassica juncea in combination with organic amendments

Background and aims The high metal bioavailability and the poor conditions of mine soils yield a low plant biomass, limiting the application of phytoremediation techniques. A greenhouse experiment was performed to evaluate the effects of organic amendments on metal stabilization and the potential of Brassica juncea L. for phytostabilization in mine soils. Methods Plants were grown in pots filled with soils collected from two mine sites located in Central Spain mixed with 0, 30 and 60 tha?1 of pine bark compost and horse- and sheep-manure compost. Plant biomass and metal concentrations in roots and shoots were measured. Metal bioavailability was assessed using a rhizosphere-based method (rhizo), which consists of a mixture of low-molecular-weight organic acids to simulate root exudates. Results Manure reduced metal concentrations in shoots (10?50 % reduction of Cu and 40?80 % of Zn in comparison with non-amended soils), bioconcentration factor (10?50 % of Cu and 40?80 % of Zn) and metal bioavailability in soil (40?50 % of Cu and 10?30 % of Zn) due to the high pH and the contribution of organic matter. Manure improved soil fertility and was also able to increase plant biomass (5?20 times in shoots and 3?30 times in roots), which resulted in a greater amount of metals removed from soil and accumulated in roots (increase of 2?7 times of Cu and Zn). Plants grown in pine bark treatments and in non-amended soils showed a limited biomass and high metal concentrations in shoots. Conclusions The addition of manure could be effective for the stabilization of metals and for enhancing the phytostabilization ability of B. juncea in mine soils. In this study, this species resulted to be a potential candidate for phytostabilization in combination with manure, differing from previous results, in which B. juncea had been recognized as a phytoextraction plant

Análisis de los destinos turísticos del futuro

Actualmente, el mayor reto del mercado turístico es cubrir la demanda de viajeros que buscan nuevos destinos turísticos, y que a la vez desean experimentar una innovación tecnológica en ellos. El turismo espacial, se ha convertido en uno de los sectores más llamativos pertenecientes a la industria turística, y por ello las empresas relacionadas a este campo buscan satisfacer las futuras necesidades de los clientes a quienes van dirigidas sus actividades, e implementar un nuevo modelo de viaje que trabaje con los avances tecnológicos para así conocer el espacio exterior. En este artículo se analiza el impacto de las formas innovadoras de viaje actuales, y las que próximamente serán establecidas, basándose en los factores de brindar comodidad, seguridad y una experiencia completamente nueva a los clientes sin desaprovechar las nuevas tecnologías, para así lograr el reconocimiento del avance de la industria turística. Los resultados preliminares de esta investigación permiten evidenciar el número de proyectos que buscan ser implementados y lanzados al público, con relación a los destinos turísticos en el espacio exterior, y la posibilidad de lograr salir de este planeta de forma más asequible para los viajeros alrededor del mundo

Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions

Item does not contain fulltextMicroscale thermophoresis (MST) allows for quantitative analysis of protein interactions in free solution and with low sample consumption. The technique is based on thermophoresis, the directed motion of molecules in temperature gradients. Thermophoresis is highly sensitive to all types of binding-induced changes of molecular properties, be it in size, charge, hydration shell or conformation. In an all-optical approach, an infrared laser is used for local heating, and molecule mobility in the temperature gradient is analyzed via fluorescence. In standard MST one binding partner is fluorescently labeled. However, MST can also be performed label-free by exploiting intrinsic protein UV-fluorescence. Despite the high molecular weight ratio, the interaction of small molecules and peptides with proteins is readily accessible by MST. Furthermore, MST assays are highly adaptable to fit to the diverse requirements of different biomolecules, such as membrane proteins to be stabilized in solution. The type of buffer and additives can be chosen freely. Measuring is even possible in complex bioliquids like cell lysate allowing close to in vivo conditions without sample purification. Binding modes that are quantifiable via MST include dimerization, cooperativity and competition. Thus, its flexibility in assay design qualifies MST for analysis of biomolecular interactions in complex experimental settings, which we herein demonstrate by addressing typically challenging types of binding events from various fields of life science